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Biochemistry & Molecular Biology
Mauricio Montenegro, Lucas Bayones, Jose Moya-Diaz, Cecilia Borassi, Andres Martin Toscani, Luciana I. Gallo, Fernando D. Marengo
Summary: The study shows that replenishment of immediately releasable pool (IRP) in chromaffin cells consists of a slow and a rapid component, linked respectively to fast endocytosis and cytosolic Ca2+ concentration. Furthermore, the stability of cortical F-actin affects the performance of both rapid replenishment and fast endocytosis.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Christiana Kontaxi, Nawon Kim, Michael A. Cousin
Summary: The protein Amph1 plays an important role in coordinating endocytosis in non-neuronal cells and synaptic vesicles at central nerve terminals. It interacts with lipids and proteins, with all interactions except for the PRD domain required for synaptic vesicle endocytosis. This study reveals that the interaction between the PRD domain of Amph1 and endophilin A1 is essential for efficient synaptic vesicle endocytosis.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Claudia Matthaeus, Kem A. Sochacki, Andrea M. Dickey, Dmytro Puchkov, Volker Haucke, Martin Lehmann, Justin W. Taraska
Summary: This study investigates the key proteins that regulate the curvature of caveolae using correlative super-resolution light and electron microscopy. The authors discover that caveolins, cavins, and EHD2 form a cohesive structural unit, regulated by intermittent associations with Pacsin2 and EHBP1, to control the curvature of caveolae.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Qiongyi Li, Yang Liu, Shujuan Xu, Kexue Zhao, Ying Ling, Rongxiu Liu, Amjad Ali, Jialin Bai
Summary: This study uncovered that caveolin-1, dynamin, and actin-dependent endocytosis pathways are necessary for EMCV infection in vitro. Overexpression of caveolin-1 was positively correlated with EMCV infection, and caveolin-1 was found to be required at the early stage of EMCV infection through confocal microscopy analysis and internalization assay.
Article
Plant Sciences
Deng Chen, Hong Hu, Wenhui He, Shimei Zhang, Mengxi Tang, Shikun Xiang, Caiyun Liu, Xuan Cai, Ahmed Hendy, Muhammad Kamran, Hao Liu, Lu Zheng, Junbing Huang, Xiao-Lin Chen, Junjie Xing
Summary: The PAL1 gene plays a crucial role in endocytosis in the plant-pathogenic fungus Magnaporthe oryzae, regulating appressorium formation and maturation. Through interactions with endocytosis proteins and modulation of cAMP and Pmk1 signaling pathways, Pal1 influences various cellular processes critical for successful infection.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Article
Multidisciplinary Sciences
Charlotte Calvet, Thibault Peineau, Najate Benamer, Maxence Cornille, Andrea Lelli, Baptiste Plion, Ghizlene Lahlou, Julia Fanchette, Sylvie Nouaille, Jacques Boutet de Monvel, Amrit Estivalet, Philippe Jean, Vincent Michel, Martin Sachse, Nicolas Michalski, Paul Avan, Christine Petit, Didier Dulon, Saaid Safieddine
Summary: This study found that SNAP-25 is essential for normal hearing function by ensuring exocytosis and maintenance of ribbon synapses in IHCs.
Article
Biology
Ke Xu, Xianwei Su, Kailun Fang, Yue Lv, Tao Huang, Mengjing Li, Ziqi Wang, Yingying Yin, Tahir Muhammad, Shangming Liu, Xiangfeng Chen, Jing Jiang, Jinsong Li, Wai-Yee Chan, Jinlong Ma, Gang Lu, Zi-Jiang Chen, Hongbin Liu
Summary: This study generated Ssh2 knockout and HA-tagged Ssh2 knock-in mice to investigate the functions of Slingshot phosphatase 2 (SSH2) in spermatogenesis. The results showed that SSH2 acts as a regulator of actin remodeling and is essential for acrosome biogenesis and male fertility. In Ssh2 knockout mice, spermatogenesis was arrested at the early spermatid stage with defective transport/fusion of proacrosomal vesicles and disorganized F-actin structures accompanied by excessive phosphorylation of COFILIN in the testes.
Article
Cell Biology
Bo Shi, Ying-Hui Jin, Ling-Gang Wu
Summary: This study investigates the role of dynamin 1 in endocytosis and vesicle size regulation. The results show that knockout of dynamin 1 slows down endocytosis and increases the diameter of synaptic vesicles, suggesting an important function of dynamin 1 in controlling vesicle size during pit formation.
Article
Cell Biology
Ross T. A. Pedersen, Aaron Snoberger, Serapion Pyrpassopoulos, Daniel Safer, David G. Drubin, E. Michael Ostap
Summary: The study demonstrates that the yeast myosin-1 Myo5 is more likely to generate power than to serve as a force-sensitive anchor in cells. This finding has implications for Myo5's role in clathrin-mediated endocytosis.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marine Laporte, Kwang Chi, Laura Caudal, Na Zhao, Yvonne Schwarz, Marta Rolland, Jose Martinez-Hernandez, Magalie Martineau, Christine Chatellard, Eric Denarier, Vincent Mercier, Florent Lemaitre, Beatrice Blot, Eve Moutaux, Maxime Cazorla, David J. Perrais, Fabien Lante, Dieter Bruns, Sandrine Fraboulet, Fiona Hemming, Frank Kirchhoff, Remy Sadoul
Summary: This study shows that Alix is involved in regulating bulk endocytosis at synapses during high frequency stimulation, and impairment of this process due to the lack of Alix results in abnormal synaptic recovery and plasticity.
Article
Endocrinology & Metabolism
Katrina Viloria, Daniela Nasteska, Julia Ast, Annie Hasib, Federica Cuozzo, Silke Heising, Linford J. B. Briant, Martin Hewison, David J. Hodson
Summary: GC-globulin (GC), also known as vitamin D-binding protein, plays a multifunctional role in transporting vitamin 25(OH)D, fatty acids, and scavenging actin. It is highly localized to glucagon-secreting alpha-cells in the pancreatic islets. Loss of GC exacerbates alpha-cell failure during metabolic stress, leading to impaired glucagon secretion and changes in alpha-cell morphology. Restoring GC levels rescues the impairments in glucagon secretion and restores the F-actin cytoskeleton of alpha-cells. Decreased GC levels in alpha-cells of type 2 diabetes donors are associated with changes in alpha-cell mass, morphology, and glucagon expression.
Article
Cell Biology
Luka Sheppard, David G. Green, Gerald Lerchbaumer, Katheryn E. Rothenberg, Rodrigo Fernandez-Gonzalez, Ulrich Tepass
Summary: The M region of alpha-Catenin plays a regulatory role in cell adhesion by recruiting interaction partners to support intercellular connections. Different levels of tension at cell contacts lead to differential recruitment of Vinculin, Ajuba, and Canoe, influencing cell adhesion.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Callista B. Harper, Eva-Maria Blumrich, Michael A. Cousin
Summary: The study highlights the crucial role of the cytoplasmic C terminus of synaptophysin (Syp) in facilitating the accurate retrieval of synaptobrevin-II (SybII) during synaptic vesicle endocytosis. Neurons lacking Syp show defects in SybII retrieval, emphasizing the importance of a conformational change within the Syp C terminus for proper binding and retrieval. Importantly, the study suggests that Syp's presynaptic role is specifically related to the control of SybII retrieval, without affecting the kinetics of synaptic vesicle endocytosis at physiological temperatures.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Xingyu Zhang, Li Zou, Lanxia Meng, Min Xiong, Lina Pan, Guiqin Chen, Yongfa Zheng, Jing Xiong, Zhihao Wang, Duc M. Duong, Zhaohui Zhang, Xuebing Cao, Tao Wang, Li Tang, Keqiang Ye, Zhentao Zhang
Summary: Amphiphysin I plays a crucial role in Alzheimer's disease, mediating tau hyperphosphorylation and synaptic dysfunction. Cleavage of Amphiphysin I by AEP leads to detrimental effects in the molecular mechanisms involved.
Article
Biochemistry & Molecular Biology
Iwona I. Smaczynska-de Rooij, Christopher J. Marklew, Ellen G. Allwood, Sarah E. Palmer, Wesley I. Booth, Ritu Mishra, Martin W. Goldberg, Kathryn R. Ayscough
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Immunology
Christina J. Gross, Ritu Mishra, Katharina S. Schneider, Guillaume Medard, Jennifer Wettmarshausen, Daniela C. Dittlein, Hexin Shi, Oliver Gorka, Paul-Albert Koenig, Stephan Fromm, Giovanni Magnani, Tamara Cikovic, Lara Hartjes, Joachim Smollich, Avril A. B. Robertson, Matthew A. Cooper, Marc Schmidt-Supprian, Michael Schuster, Kate Schroder, Petr Broz, Claudia Traidl-Hoffmann, Bruce Beutler, Bernhard Kuster, Juergen Ruland, Sabine Schneider, Fabiana Perocchi, Olaf Gross
Review
Cell Biology
Martin W. Goldberg
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Ines J. de Castro, James Budzak, Maria L. Di Giacinto, Lorena Ligammari, Ezgi Gokhan, Christos Spanos, Daniela Moralli, Christine Richardson, Jose I. de las Heras, Silvia Salatino, Eric C. Schirmer, Katharine S. Ullman, Wendy A. Bickmore, Catherine Green, Juri Rappsilber, Sarah Lamble, Martin W. Goldberg, Veronica Vinciotti, Paola Vagnarelli
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Katharina S. Schneider, Christina J. Gross, Roland F. Dreier, Benedikt S. Saller, Ritu Mishra, Oliver Gorka, Rosalie Heilig, Etienne Meunier, Mathias S. Dick, Tamara Cikovic, Jan Sodenkamp, Guillaume Medard, Ronald Naumann, Juergen Ruland, Bernhard Kuster, Petr Broz, Olaf Gross
Article
Geriatrics & Gerontology
Mehmet U. Bikkul, Craig S. Clements, Lauren S. Godwin, Martin W. Goldberg, Ian R. Kill, Joanna M. Bridger
Article
Developmental Biology
Florian Geisler, Richard A. Coch, Christine Richardson, Martin Goldberg, Bernd Denecke, Olaf Bossinger, Rudolf E. Leube
Article
Ophthalmology
Antal Tapodi, Daniel M. Clemens, Alice Uwineza, Miguel Jarrin, Martin W. Goldberg, Emmanuelle Thinon, William P. Heal, Edward W. Tate, Karinne Nemeth-Cahalan, Irene Vorontsova, James E. Hall, Roy A. Quinlan
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Cell Biology
Natalia Saiz-Ros, Rafal Czapiewski, Ilaria Epifano, Andrew Stevenson, Selene K. Swanson, Charles R. Dixon, Dario B. Zamora, Marion McElwee, Swetha Vijayakrishnan, Christine A. Richardson, Li Dong, David A. Kelly, Lior Pytowski, Martin W. Goldberg, Laurence Florens, Sheila V. Graham, Eric C. Schirmer
Article
Cell Biology
Marianne Grafe, Petros Batsios, Irene Meyer, Daria Lisin, Otto Baumann, Martin W. Goldberg, Ralph Graef
Article
Biochemistry & Molecular Biology
Christina James, Marret Mueller, Martin W. Goldberg, Christof Lenz, Henning Urlaub, Ralph H. Kehlenbach
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Georgia E. Hulmes, John D. Hutchinson, Noa Dahan, James M. Nuttall, Ellen G. Allwood, Kathryn R. Ayscough, Ewald H. Hettema
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Eve Hunter-Featherstone, Natalie Young, Kathryn Chamberlain, Pablo Cubillas, Ben Hulette, Xingtao Wei, Jay P. Tiesman, Charles C. Bascom, Adam M. Benham, Martin W. Goldberg, Gabriele Saretzki, Iakowos Karakesisoglou
Summary: The study demonstrates that extracellular mechanics have significant effects on keratinocyte cell biology and should be utilized in skin research to ensure successful production of physiologically relevant skin models.
Article
Multidisciplinary Sciences
Charles R. Dixon, Poonam Malik, Jose de Las Heras, Natalia Saiz-Ros, Flavia de Lima Alves, Mark Tingey, Eleanor Gaunt, A. Christine Richardson, David A. Kelly, Martin W. Goldberg, Greg J. Towers, Weidong Yang, Juri Rappsilber, Paul Digard, Eric C. Schirmer
Summary: STING is an adaptor protein involved in cytoplasmic DNA sensing through cGAMP/cGAS, triggering innate immune responses. Upon transfection of dsDNA or dsRNA mimics, STING can redistribute from the inner nuclear membrane to the outer nuclear membrane, interacting with DNA- and RNA-binding proteins.