Integrating high-throughput screening and sequencing for monoclonal antibody discovery and engineering

Quantifiable predictive features define epitope-specific T cell receptor repertoires

(2017) Pradyot Dash et al.   NATURE

Identifying specificity groups in the T cell receptor repertoire

(2017) Jacob Glanville et al.   NATURE

Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies

(2016) Bryan Briney et al.   CELL

Neutralizing human antibodies prevent Zika virus replication and fetal disease in mice

(2016) Gopal Sapparapu et al.   NATURE

Ultra-high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes

(2016) Jonathan R McDaniel et al.   Nature Protocols

Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates

(2016) Kendrick B. Turner et al.   PLoS One

Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires

(2016) Brandon J. DeKosky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

(2016) Mark Pogson et al.   Nature Communications

Accurate and predictive antibody repertoire profiling by molecular amplification fingerprinting

(2016) T. A. Khan et al.   Science Advances

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Deep sequencing in library selection projects: what insight does it bring?

(2015) J Glanville et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Direct high-throughput amplification and sequencing of immunoglobulin genes from single human B cells

(2015) Rajagopal Murugan et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Isolation and characterization of antigen-specific alpaca (Lama pacos) VHH antibodies by biopanning followed by high-throughput sequencing

(2015) Nobuo Miyazaki et al.   JOURNAL OF BIOCHEMISTRY

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

(2015) Dongmei Hu et al.   PLoS One

High-throughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum

(2015) Anders Christiansen et al.   Scientific Reports

Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining

(2015) Bo Wang et al.   Scientific Reports

High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination

(2014) Yann-Chong Tan et al.   CLINICAL IMMUNOLOGY

The promise and challenge of high-throughput sequencing of the antibody repertoire

(2014) George Georgiou et al.   NATURE BIOTECHNOLOGY

Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery

(2014) E-Chiang Lee et al.   NATURE BIOTECHNOLOGY

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Towards error-free profiling of immune repertoires

(2014) Mikhail Shugay et al.   NATURE METHODS

A robust pipeline for rapid production of versatile nanobody repertoires

(2014) Peter C Fridy et al.   NATURE METHODS

IMGT®, the international ImMunoGeneTics information system® 25 years on

(2014) Marie-Paule Lefranc et al.   NUCLEIC ACIDS RESEARCH

Identification and characterization of the constituent human serum antibodies elicited by vaccination

(2014) Jason J. Lavinder et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice

(2014) A. J. Murphy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-resolution antibody dynamics of vaccine-induced immune responses

(2014) Uri Laserson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

From deep sequencing to actual clones

(2014) S. D'Angelo et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Barcode-Enabled Sequencing of Plasmablast Antibody Repertoires in Rheumatoid Arthritis

(2014) Yann-Chong Tan et al.   Arthritis & Rheumatology

Development of humanized rabbit monoclonal antibodies against vascular endothelial growth factor receptor 2 with potential antitumor effects

(2013) Yanlan Yu et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Single-cell based high-throughput sequencing of full-length immunoglobulin heavy and light chain genes

(2013) Christian E. Busse et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Specific binder for Lightning-Link® biotinylated proteins from an antibody phage library

(2013) Fortunato Ferrara et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Comprehensive Interrogation of a Minimalist Synthetic CDR-H3 Library and Its Ability to Generate Antibodies with Therapeutic Potential

(2013) Ciara M. Mahon et al.   JOURNAL OF MOLECULAR BIOLOGY

Deep sequencing of phage display libraries to support antibody discovery

(2013) Ulla Ravn et al.   METHODS

High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire

(2013) Brandon J DeKosky et al.   NATURE BIOTECHNOLOGY

Molecular deconvolution of the monoclonal antibodies that comprise the polyclonal serum response

(2013) Y. Wine et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetic measurement of memory B-cell recall using antibody repertoire sequencing

(2013) C. Vollmers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains

(2013) J. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo identification of VRC01 class HIV-1-neutralizing antibodies by next-generation sequencing of B-cell transcripts

(2013) J. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lineage Structure of the Human Antibody Repertoire in Response to Influenza Vaccination

(2013) N. Jiang et al.   Science Translational Medicine

Using synthetic templates to design an unbiased multiplex PCR assay

(2013) Christopher S. Carlson et al.   Nature Communications

Wrestling with the repertoire: The promise and perils of next generation sequencing for antigen receptors

(2012) Paul D. Baum et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

A proteomics approach for the identification and cloning of monoclonal antibodies from serum

(2012) Wan Cheung Cheung et al.   NATURE BIOTECHNOLOGY

Transferring the Characteristics of Naturally Occurring and Biased Antibody Repertoires to Human Antibody Libraries by Trapping CDRH3 Sequences

(2012) Sophie Venet et al.   PLoS One

Construction of a rationally designed antibody platform for sequencing-assisted selection

(2012) H. B. Larman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody isolation from immunized animals: comparison of phage display and antibody discovery via V gene repertoire mining

(2012) I. Saggy et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Phage display screening without repetitious selection rounds

(2011) Peter A.C. ’t Hoen et al.   ANALYTICAL BIOCHEMISTRY

Synthetic Antibodies Designed on Natural Sequence Landscapes

(2011) Wenwu Zhai et al.   JOURNAL OF MOLECULAR BIOLOGY

Broad neutralization coverage of HIV by multiple highly potent antibodies

(2011) Laura M. Walker et al.   NATURE

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Phenotype-information-phenotype cycle for deconvolution of combinatorial antibody libraries selected against complex systems

(2011) H. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells

(2010) Sai T Reddy et al.   NATURE BIOTECHNOLOGY

By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection

(2010) U. Ravn et al.   NUCLEIC ACIDS RESEARCH

Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire

(2009) J. Glanville et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Throughput Sequencing of the Zebrafish Antibody Repertoire

(2009) J. A. Weinstein et al.   SCIENCE

Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing

(2009) S. D. Boyd et al.   Science Translational Medicine

Submitting antibodies to binding arbitration

(2008) Stephen W Michnick et al.   Nature Chemical Biology