Article
Plant Sciences
Yehao Zhang, Lan Miao, Qing Peng, Xiaodi Fan, Wenting Song, Bin Yang, Peng Zhang, Guangyu Liu, Jianxun Liu
Summary: The study demonstrates that Parthenolide (PTL) can regulate neuroinflammation and protect against ischemic brain injury by modulating microglial polarization via the RhoA/ROCK pathway.
Article
Clinical Neurology
Zhijuan Cao, Sean S. Harvey, Terrance Chiang, Aulden G. Foltz, Alex G. Lee, Michelle Y. Cheng, Gary K. Steinberg
Summary: This study investigated the spatiotemporal changes in the secondary degenerative thalamus post-stroke, revealing early microglial activation and later neurodegeneration. Transcriptome analysis at PD28 showed a higher number of differentially expressed genes in the thalamus, with neuroinflammation being the top activated pathway and microglia the most enriched cell type. Additionally, a unique subtype of microglia (CD11c(+)) with features of neurodegenerative disease-associated microglia was identified in the degenerative thalamus after stroke.
Article
Plant Sciences
Yan Mi, Kun Jiao, Ji-kai Xu, Kun Wei, Jing-yu Liu, Qing-qi Meng, Ting-ting Guo, Xue-ni Zhang, Di Zhou, De-gang Qing, Yu Sun, Ning Li, Yue Hou
Summary: The study explored the effect of kellerin on cerebral ischemia and its possible mechanisms using MCAO model and LPS-activated microglia model. Kellerin improved neurological outcome, reduced brain infarct size, and alleviated neuron damage by inhibiting microglial activation under pathologic conditions of focal cerebral ischemia. In in vitro studies, kellerin protected neuronal cells from damage by suppressing pro-inflammatory cytokines, NF-kappa B signaling pathway, ROS generation, and NADPH oxidase activity.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Neurosciences
Hao Wang, Jingjing Li, Han Zhang, Mengyao Wang, Lifang Xiao, Yitong Wang, Qiong Cheng
Summary: Stroke is the second leading cause of death and disability globally. Microglia, innate immune cells in the brain, play a critical role in the neuroinflammatory response following ischemic injury. The regulation of microglia phenotype is essential in controlling the neuroinflammatory reaction and secondary injury in ischemic stroke.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Critical Care Medicine
Likai Shi, Baowei Li, Guoqing Chen, Yingsi Huang, Zhenpu Tian, Lifeng Zhang, Li Tian, Qiang Fu
Summary: MEF2D plays an inhibitory role in the activation of microglia under oxygen-glucose deprivation and reoxygenation conditions, reducing neuronal injury by regulating the expression of NF-kappa b, TLR, and cytokines. The overexpression of MEF2D improves microglial activation, neuroinflammatory response, mitochondrial dysfunction, brain injury, and cognitive function in cerebral ischemia, while MEF2D siRNA treatment exacerbates these indicators.
Article
Biochemistry & Molecular Biology
Wenchao Cheng, Yuhan Wang, Lan Zhang, Chang Cheng, Xiuying Chen, Wen Huang
Summary: This study demonstrates that dipyridamole facilitates the conversion of DAM to the anti-inflammatory phenotype through the ENT2/Adora2a pathway and inhibits the activation of the ERK1/2 and NF-kappa B signaling pathways, thereby alleviating neuroinflammation in WMLs.
NEUROCHEMICAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Lalita Subedi, Bhakta Prasad Gaire
Summary: The search for novel therapeutic agents for cerebral ischemia/stroke has led to a focus on neuroprotective phytochemicals with anti-inflammatory properties as potential drug candidates. Studies have shown that these compounds reduce brain inflammation by decreasing proinflammatory microglia and macrophages while promoting anti-inflammatory responses, leading to improved outcomes in animal stroke models.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Clinical Neurology
Lexiao Li, Nelufar Yasmen, Ruida Hou, Seyoung Yang, Jae Yeol Lee, Jiukuan Hao, Ying Yu, Jianxiong Jiang
Summary: The study demonstrates that novel compounds inhibiting mPGES-1 can alleviate neuroinflammation and brain injury after cerebral ischemia, suggesting mPGES-1 as a feasible therapeutic target for ischemic stroke.
Article
Medicine, Research & Experimental
Lin Li, Weifeng Jiang, Baojian Yu, Huiqi Liang, Shihui Mao, Xiaowei Hu, Yan Feng, Jiadong Xu, Lisheng Chu
Summary: Quercetin promotes microglia/macrophage M2 polarization in the treatment of ischemic stroke by modulating the PI3K/Akt/NF-κB signaling pathway, thereby improving neurological deficits and reducing infarct volume.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Xue Zhao, Junde Zhu, Shan Chen, Ruojing Liu, Tingting Long
Summary: The study aimed to investigate the effect of neural stem cell-derived exosomes (NSC-Exos) on neural function after rat cerebral ischemia-reperfusion injury by regulating microglia-mediated inflammatory response. The results showed that NSC-Exos can promote the polarization of microglia, reduce microglia-mediated neuroinflammation, and may be a strategy for the treatment of microglia-mediated neuroinflammation after ischemic brain injury.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Geriatrics & Gerontology
Ning Kang, Yuanyuan Shi, Jiaxi Song, Fei Gao, Mingyue Fan, Wei Jin, Yaran Gao, Peiyuan Lv
Summary: Inflammatory responses induced by chronic cerebral hypoperfusion play a critical role in the progression of vascular dementia. STING signaling is a key mediator of inflammation and immunological responses in the CNS, while resveratrol has potent anti-inflammatory effects. This study demonstrates that the STING/TBK1/IRF3 pathway may be critical for proinflammatory responses in cerebral tissue with persistent hypoperfusion, and resveratrol exerts its anti-inflammatory effects by suppressing STING/TBK1/IRF3 signaling.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Immunology
Yao Zhao, Jiawei Zhang, Yaling Zheng, Yaxuan Zhang, Xiao Jie Zhang, Hongmei Wang, Yu Du, Jian Guan, Xiuzhe Wang, Jianliang Fu
Summary: NAD(+) administration showed protective effects on cognitive impairment and neuroinflammation in chronic cerebral hypoperfusion models in vivo and in vitro, by activating the Sirt1/PGC-1 alpha pathway for mitochondrial protection and ROS inhibition.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Immunology
Chronic cerebral Hypoperfusion, Vascular Dementia, Yaling Zheng, Jiawei Zhang, Yao Zhao, Yaxuan Zhang, Xiaojie Zhang, Jian Guan, Yu Liu, Jianliang Fu
Summary: Findings suggest that curcumin shows promising therapeutic effects on cognitive impairments induced by diabetes and chronic cerebral hypoperfusion, potentially through suppressing neuroinflammation and reducing cell apoptosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kuang Zheng, Yuliang Zhang, Chengwei Zhang, Wangyang Ye, Chenxing Ye, Xianxi Tan, Ye Xiong
Summary: PRMT8 regulates the activation and polarization of microglia, inhibits neuronal apoptosis, and ameliorates cerebral ischemia/reperfusion injury.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Neurosciences
Yusufu Mahemuti, Kaheerman Kadeer, Riqing Su, Abudureheman Abula, Yirizhati Aili, Aierpati Maimaiti, Subinuer Abulaiti, Maimaitiyasheng Maimaitituerxun, Tong Miao, Shihao Jiang, Aximujiang Axier, Maimaitili Aisha, Yongxin Wang, Xiaojiang Cheng
Summary: TSPO expression is associated with CIRI and may affect neuroinflammatory response by regulating autophagy dysfunction, indicating its potential importance in the treatment of ischemic stroke.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Anup Bhusal, Md Habibur Rahman, Kyoungho Suk
Summary: The hypothalamus is a critical brain region for energy homeostasis regulation, with recent studies highlighting the role of glial cells in addition to neurons. However, inflammatory activation of these cells under metabolic stress conditions contributes to various metabolic diseases, worsening the disease state.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Editorial Material
Cell Biology
Ruqayya Afridi, Makoto Tsuda, Hoon Ryu, Kyoungho Suk
Article
Neurosciences
In-Sun Choi, Jae-Hong Kim, Ji-Young Jeong, Maan-Gee Lee, Kyoungho Suk, Il-Sung Jang
Summary: This study reveals a novel mechanism involving astrocyte-neuron interactions in sleep regulation, where ATP derived from astrocytes is converted into adenosine by TNAP. Adenosine inhibits non-sleep-promoting neurons and excites sleep-promoting neurons, thereby affecting sleep-wake cycle. This finding has important implications for understanding the physiological basis of sleep regulation.
Article
Neurosciences
Jae-Hong Kim, Osung Kwon, Anup Bhusal, Jiyoun Lee, Eun Mi Hwang, Hoon Ryu, Jae-Yong Park, Kyoungho Suk
Summary: This study reports the generation and characterization of conditional transgenic mice for lipocalin-2 (LCN2) expression. By delivering Cre recombinase under the control of a GFAP promoter, targeted overexpression of LCN2 in astrocytes of the hippocampus was achieved. These mice showed neuroinflammation, enhanced glial activation, increased proinflammatory cytokines, and impaired cognitive functions, providing a new in vivo model for neuroinflammation.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Clinical Neurology
Jae-Hong Kim, Ri Jin Kang, Seung Jae Hyeon, Hoon Ryu, Hyejin Joo, Youngmin Bu, Jong-Heon Kim, Kyoungho Suk
Summary: Reactive glial cells play a role in brain injury, but their contribution to secondary inflammatory pathology and neurological deficits is poorly understood. This study investigates the role of Lipocalin-2 (LCN2) in neuroinflammation following brain injury. LCN2 expression is high in the mouse brain after controlled cortical impact (CCI) and photothrombotic stroke (PTS) injury. It is also significantly higher in patients with chronic traumatic encephalopathy (CTE). Astrocytes are the major source of LCN2 in the injured brain. Deficiency of LCN2 or injection of an LCN2 neutralizing antibody reduces brain lesions, behavioral deficits, and neuroinflammation. In cultured glial cells, LCN2 enhances proinflammatory cytokine gene expression and inhibits neurotropic factors expression. Overall, LCN2 mediates secondary damage response to traumatic and ischemic brain injury by promoting neuroinflammation and suppressing the expression of neurotropic factors.
Article
Biochemistry & Molecular Biology
Md. Sekendar Ali, Eon-Bee Lee, Suk-Kyung Lim, Kyoungho Suk, Seung-Chun Park
Summary: We isolated and characterized Limosilactobacillus reuteri PSC102 and evaluated its probiotic, antioxidant, and antibacterial properties. We selected eight isolates from pig feces and identified L. reuteri PSC102 using 16S rRNA gene sequencing. We tested its probiotic, antioxidant, and antibacterial properties using various assays.
Article
Medicine, General & Internal
Narae Park, Chanchal Sharma, Un Ju Jung, Sehwan Kim, Youngpyo Nam, Kyung-Suk Kim, Kyoungho Suk, Ho-Won Lee, Sang Ryong Kim
Summary: This study investigated the therapeutic effects of transplanting hMSCs into wild-type mice with Ara-C-induced CA. The hMSC-treated mice showed improved motor and balance coordination, preserved cerebellar neuronal loss, and increased neurotrophic factors levels. hMSC implantation also suppressed proinflammatory responses. These results suggest that hMSCs have therapeutic potential for treating Ara-C-induced CA by protecting neurons and inhibiting inflammation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell Biology
Ruqayya Afridi, Kyoungho Suk
Summary: Chronic stress is a significant risk factor for psychiatric diseases, particularly depression, as it causes cellular and structural changes that affect neurocircuitry and contribute to the development of depression. Recent studies have shown that microglial cells play a role in stress-induced depression through inflammatory activation. The exact pathways involved in stress-induced microglial activation are still unclear, but identifying these triggers can aid in the development of targeted therapies for depression.
Article
Biology
Jong-Heon Kim, Jin Han, Ruqayya Afridi, Jae-Hong Kim, Md Habibur Rahman, Dong Ho Park, Won Suk Lee, Gyun Jee Song, Kyoungho Suk
Summary: We conducted a multiplexed kinome-wide siRNA screen to identify kinases that regulate various inflammatory phenotypes of mouse glial cells. Our experiments showed the importance of T-cell receptor signaling components in microglial activation and a metabolic shift in astrocyte migration. This time- and cost-effective screen provides new insight into the regulation of glial cells and neuroinflammation, and potential drug targets for inflammatory diseases and cancer.
LIFE SCIENCE ALLIANCE
(2023)
Review
Biochemical Research Methods
Jong-Heon Kim, Ruqayya Afridi, Won-Ha Lee, Kyoungho Suk
Summary: This review provides an updated overview of proteomic analysis of glia in the context of Alzheimer's disease (AD) and discusses challenges and cutting-edge proteomic technologies in the field. Unbiased comprehensive analysis of glial proteomes aids our understanding of the molecular and cellular mechanisms of AD pathogenesis, highlighting the crucial role of glial cells and providing novel insights into the mechanisms of AD pathology. A deeper understanding of the AD-related glial proteome could offer a repertoire of potential biomarkers and therapeutics.
EXPERT REVIEW OF PROTEOMICS
(2023)
Editorial Material
Cell Biology
Ruqayya Afridi, Anup Bhusal, Makoto Tsuda, Hoon Ryu, Kyoungho Suk
Review
Microbiology
Md. Sekendar Ali, Eon-Bee Lee, Walter H. H. Hsu, Kyoungho Suk, Syed Al Jawad Sayem, H. M. Arif Ullah, Seung-Jin Lee, Seung-Chun Park
Summary: Probiotics are used as an alternative to antibiotics in feed/food supplements. They have been shown to provide various health benefits such as preventing diarrhea, irritable bowel syndrome, and immunomodulation. Besides live probiotics, there is growing interest in using inactivated probiotics in pigs, with studies showing that both forms can boost immunity, improve growth performance, and decrease the incidence of diarrhea.
Review
Biochemistry & Molecular Biology
Hyeung-Seob Shin, Jae-Joon Shin, Jeongkwang Park, Imene Arab, Kyoungho Suk, Won-Ha Lee
Summary: Long noncoding RNAs (lncRNAs) are >200 bases long and lack protein-coding functions. They are involved in signal transduction and gene expression regulation through interaction with proteins or RNAs, and exhibit various functions. This review summarizes the current knowledge on lncRNAs expressed in macrophages, focusing on their molecular targets and the regulated biological processes during the development of inflammatory diseases. The potential application of this information in lncRNA-targeting therapy is also discussed.
Review
Biochemical Research Methods
Ruqayya Afridi, Won-Ha Lee, Jong-Heon Kim, Kyoungho Suk
Summary: Astrocyte secretomics is an important field for understanding the functions of the central nervous system. Proteomics databases play a crucial role in studying the astrocyte secretome. However, dedicated databases specifically focused on astrocyte secretome are currently limited. Developing such databases is essential for further advancements in this field.
EXPERT REVIEW OF PROTEOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Sekendar Ali, Biruk Tesfaye Birhanu, Eon-Bee Lee, Yixian Quah, Naila Boby, Kyoungho Suk, Sam-Pin Lee, Seung-Jin Lee, Seung-Chun Park
Summary: This study investigated the immunomodulatory effects of fermented soybean extract (FSE) in cells and mice. FSE significantly reduced the expression of proinflammatory cytokines in cells and enhanced splenocyte proliferation, neutrophil migration, phagocytic activities, and the expression of specific cells and cytokines in mice. The identified compounds in FSE were found to potentially bind to the human high-affinity Fc gamma RI-IgG complex. FSE has the potential to be used as an ingredient in health functional food with enhanced immunomodulating ability.
FOOD BIOTECHNOLOGY
(2022)