期刊
GENETICS AND MOLECULAR BIOLOGY
卷 40, 期 4, 页码 824-833出版社
SOC BRASIL GENETICA
DOI: 10.1590/1678-4685-GMB-2017-0053
关键词
Medicago sativa L.; RNA-seq; freezing tolerance; C-repeat binding factors (CBF)
资金
- National Natural Science Foundation of China [31302019, 31470571]
- China Postdoctoral Science Foundation [2016T90307, 2015M571430]
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT)
- MOST [2013AA102607-5]
- Heilongjiang Province Postdoctoral Science Foundation [LBH-Z14126]
Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 degrees C) and freezing (-8 degrees C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying their important roles in response to cold and freezing stresses. Furthermore, nine CBF transcripts differentially expressed were homologous to CBF genes of Mt-FTQTL6 site, conferring freezing tolerance in M. truncatula, which indicated that a genetic mechanism controlling freezing tolerance was conservative between M. truncatula and M. sativa. In summary, this transcriptome dataset highlighted the gene regulation response to cold and/or freezing stresses in alfalfa, which provides a valuable resource for future identification and functional analysis of candidate genes in determining freezing tolerance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据