期刊
FOOD CHEMISTRY
卷 218, 期 -, 页码 1-8出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.08.106
关键词
Casein peptide; Molecular weight (MW); Transepithelial transport; Brush-border peptidase (BBP); Hydrolysis; Bioavailability
资金
- National Natural Science Foundation of China (NSFC) [31271846]
- Program for New Century Excellent Talents in the University of Ministry of Education of China [2010JS078]
- Chinese Universities Scientific Fund [2016SP003]
The transepithelial transport routes of casein-derived peptides with different molecular weights (MWs) were investigated using a Caco-2 cell monolayer. The peptidase hydrolysis during transport was also studied. The results indicate that the paracellular route was the main pathway for F1 (1600-1300 Da) and F2 (1000-500 Da), and the bioavailabilities were 10.66% and 9.54%, respectively. Peptidase hydrolysis results reveal that brush-border peptidases (BBPs) as well as some other peptidases were responsible for peptide degradation in the paracellular route. The maximum hydrolysis rate of the former was 6.91 and 5.59 mu M Gly/min for the latter. However, PepT1 was involved in the transport of F3 (<500 Da) and its bioavailability was 16.23%. BBPs were the main peptidases involved in the PepT1 transport and the maximum hydrolysis rate was 11.4 mu M Gly/min. Furthermore, we found that the amino acid sequence of di- and tripeptides might affect their bioavailabilities significantly. (C) 2016 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据