期刊
FOOD CHEMISTRY
卷 214, 期 -, 页码 259-268出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.06.100
关键词
Phenolic compounds; Lipase, alpha-Glucosidase and alpha-amylase inhibition
资金
- Mississippi Agricultural and Forestry Experiment Station
- USDA-ARS SCA at the Mississippi State University [58-402-2729, MIS 501170]
Antioxidant-rich plant foods can inhibit starch and lipid digestions that are relevant to diabetes management. Two high-antioxidant black legumes, black soybean (Glycine max) and black turtle bean (Phaseolus vulgaris), belonging to two different genera were used to investigate their capacity against digestive enzymes. Phenolic substances were compared in crude, semi-purified extracts (semi-purified by XAD-7 column), and fractions (fractionationed by Sephadex LH-20 column) from these two legumes. In addition, their antioxidant capacities and abilities to inhibit digestive enzymes were characterized. Results showed that Fraction V from black soybean was the most effective (IC50: 0.25 mg/mL) against alpha-amylase; Fraction V from black turtle bean was the most potent (IC50: 0.25 mu g/mL) against alpha-glucosidase; Fraction IV from black turtle bean was the most powerful (IC50; 76 mu g/mL) against lipase. Of the pure phenolic compounds tested, myricetin showed the highest inhibition of alpha-amylase, alpha-glucosidase and lipase (IC50: 0.38 mg/mL, 0.87 mu g/mL and 15 mu g/mL, respectively). (C) 2016 Elsevier Ltd. All rights reserved.
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