Article
Fisheries
Zefeng Gu, Jingmiao Yang, Min Yang, Yuewen Deng, Yu Jiao
Summary: This study evaluated the effect of DAC on DNA methylation and the immune system of pearl oysters. The results showed that DAC decreased global DNA methylation level and activated immune-related genes, leading to an improvement in humoral immunity. These findings contribute to a better understanding of the mechanism underlying DNA methylation in immune regulation.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yu Shi, Xiaolan Pan, Meng Xu, Huiru Liu, Hanzhi Xu, Maoxian He
Summary: The study revealed the wide distribution and crucial role of Smad1/5 in developmental stages of P. f. martensii; Smad1/5, along with BMP2-Smad signals, plays a role in defending against bacterial and virus infections; Smad1/5 can regulate immune gene expression via the BMP2-Smads pathway.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Environmental Sciences
Bingcong Ye, Zhifeng Gu, Xingzhi Zhang, Yi Yang, Aimin Wang, Chunsheng Liu
Summary: This study compared the growth, feeding, and metabolism differences between two pearl oyster species. The results showed that P. f. martensii performed better in feeding and growth, while P. maxima had higher metabolic activity.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Chenghao Shen, Meizhen Zhang, Haiying Liang, Junjun He, Bin Zhang, Bidan Liang
Summary: In this study, PmKSPI, a four-domain Kunitz-type serine protease inhibitor, was cloned and functionally characterized in Pinctada fucata martensii. The results suggest that PmKSPI is tightly associated with the immunological defense of P. f. martensii and may have a bactericidal effect on Gram-negative bacteria.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Hua Zhang, Gaoyou Yao, Maoxian He
Summary: In this study, a novel long noncoding RNA (lncRNA7467) was identified from the pearl oyster. The lncRNA showed the highest expression in mantle tissue and was involved in the biomineralization process.
AQUACULTURE REPORTS
(2022)
Article
Environmental Sciences
Zhe Zheng, Yongshan Liao, Jianming Ye, Chuangye Yang, Linda Adzigbli, Qingheng Wang, Xiaodong Du, Yuewen Deng
Summary: The richness and diversity of pearl oyster intestinal microbiota are lower than those of surrounding water, but functionally similar. The bacterial communities in pearl oyster intestines show adaptability at the phylum and genus levels.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Environmental Sciences
Meizhen Zhang, Chenghao Shen, Haiying Liang, Yuyuan Wu, Bidan Liang
Summary: In this study, the novel PmTRAF2 and PmTRAF4 genes were cloned from P. f. martensii and their expression in different tissues was analyzed. The results showed that both genes were significantly expressed in hepatopancreas and gill. Under stimulation and surgery, the expression of TRAF genes in hepatopancreas changed significantly. The yeast two-hybrid assay revealed that PmTRAF2 interacts with PmTRAF6, suggesting its potential role in immune signaling pathways.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhe Zheng, Ruijuan Hao, Chuangye Yang, Yu Jiao, Qingheng Wang, Ronglian Huang, Yongshan Liao, Jianbo Jian, Yao Ming, Lixin Yin, Weiming He, Ziman Wang, Chuyi Li, Qi He, Kun Chen, Yuewen Deng, Xiaodong Du
Summary: Biomineralization-controlled growth of exo-/endoskeleton contributes to body growth and size diversity. This study used pearl oysters to investigate the genetic regulation of biomineralization-controlled growth and ectopic biomineralization. The insulin-like growth factor (IGF) endocrine signal and ancient single-copy transcription factors formed the regulatory network. Cross-phylum regulation of key long noncoding RNA (lncRNA) in bivalves and mammals suggests conserved genetic and epigenetic regulation. Thyroid hormone signal and apoptosis regulation affected ectopic biomineralization in pearl oysters.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Fisheries
Chenghao Shen, Haiying Liang, Zhijie Guo, Meizhen Zhang
Summary: This study focuses on the design and evaluation of structurally altered antimicrobial peptides (AMPs) derived from histones in the pearl oyster Pinctada fucata martensii. The researchers identified new genes PmH2A and PmH2B using molecular cloning approaches. They synthesized a series of synthetic analogs derived from these genes and found that AMPs derived from PmH2A and PmH4 exhibited antibacterial activity. PmH2A-derived AMPs had an α-helical structure, while PmH4-derived AMPs had an extended strand/random coil structure.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yanfei Cao, Zhixin Li, Xueru Liang, Jiayi Chen, Xinwei Xiong, Yu Jiao, Zefeng Gu, Xiaodong Du
Summary: This study identified the functions of Spd and Spm in pearl oysters, showing their important roles in immune regulation and providing references for further research on polyamine functions in mollusks.
Article
Fisheries
Meiqi Dai, Yuting Zhang, Yu Jiao, Yuewen Deng, Xiaodong Du, Chuangye Yang
Summary: This study analyzed the function and regulatory role of a novel microRNA miR-63 in the immune response of pearl oysters. The results showed that the expression of this microRNA increased after pathogen stimulation and overexpression of it resulted in decreased activity of immune and antioxidant enzymes in the serum.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Haiying Liang, Meizhen Zhang, Chenghao Shen, Junjun He, Jinzhao Lu, Zhijie Guo
Summary: This study identified a TLS-like gene, PmTLS, in Pinctada fucata martensii and investigated its role in the innate immune response. The results showed that PmTLS is highly expressed in hemocytes and can inhibit the growth of gram-negative bacteria.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Chuangye Yang, Ruijuan Hao, Chengzhang He, Yuewen Deng, Qingheng Wang
Summary: This study characterized the FAD gene in P. f. martensii and found that Pm Delta 5FAD plays an important role in regulating inflammation. Functional analysis showed that Pm Delta 5FAD has Delta 5-desaturation activity towards PUFA substrates and its expression level significantly increases on day 1 after grafting operation.
AQUACULTURE REPORTS
(2022)
Article
Environmental Sciences
Jingru Yang, Jinling Yang, Mingqiang Chen, Zhengyi Fu, Jing Sun, Gang Yu, Aimin Wang, Zhenhua Ma, Zhifeng Gu
Summary: This study investigated the physiological and biochemical changes in black and red shell Pinctada fucata under acute high and low salt stress. The results showed that red P. fucata responded more quickly to changes in salinity and exhibited reduced damage compared to black P. fucata. Additionally, P. fucata was found to be more adaptable to acute low salt environments. These findings have important implications for the selective breeding of P. fucata.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Limnology
Yu Shi, Xiaolan Pan, Meng Xu, Huiru Liu, Hanzhi Xu, Maoxian He
Summary: The study showed that Smad6 in mollusks, specifically in the pearl oyster Pinctada fucata martensii, plays a role in immunity. It has a positive effect in the immune response to challenges, but inhibits the wound healing process. Smad6 and Smad3 may have opposing functions.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Fisheries
Qingyue Chen, Wanmei Yu, Ruijuan Hao, Jingmiao Yang, Chuangye Yang, Yuewen Deng, Yongshan Liao
Summary: This study investigated the MYH gene in Pinctada fucata martensii and found its important role in muscle growth and contraction. The research also discovered the expression pattern of PmMYH in different tissues and developmental stages, as well as identified growth-related SNP markers.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Zhuoxin Lai, Wei Zhao, Yingying Lu, Lingjun Wu, Chuangye Yang, Qingheng Wang
Summary: This study characterized the cDNA of the AP-1 gene in Pinctada fucata martensii and found that it is highly expressed in multiple tissues and during specific developmental stages. The gene also shows significantly high expression under cold tolerance stress. SNP and haplotype analysis revealed that genetic variations in PmAP-1 are associated with cold tolerance in P. f. martensii.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Editorial Material
Physiology
Bruno C. Araujo, Qingheng Wang, Chuangye Yang, Erchao Li
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Fisheries
Chuangye Yang, Ruijuan Hao, Chengzhang He, Yuewen Deng, Qingheng Wang
Summary: This study characterized the FAD gene in P. f. martensii and found that Pm Delta 5FAD plays an important role in regulating inflammation. Functional analysis showed that Pm Delta 5FAD has Delta 5-desaturation activity towards PUFA substrates and its expression level significantly increases on day 1 after grafting operation.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Jiayi Chen, Jing Huang, Jiaqi Peng, Chuangye Yang, Yongshan Liao, Junhui Li, Yuewen Deng, Xiaodong Du
Summary: This study investigated the effects of hypoxia on pearl oysters by measuring the activity of enzymes involved in digestion, energy metabolism, oxidative stress regulation, and immune function. Short-term hypoxia promoted adaptation to the environment, while long-term hypoxia decreased the survival rate of pearl oysters by affecting various physiological processes.
AQUACULTURE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zhe Zheng, Zhijie Xu, Caixia Cai, Yongshan Liao, Chuangye Yang, Xiaodong Du, Ronglian Huang, Yuewen Deng
Summary: This study found that miRNAs from plants can be transported to mollusks such as pearl oysters through the food chain, and they participate in various biological processes by targeting multiple genes. These findings provide new insights into the transmission of novel nutrients through the food chain.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2022)
Article
Environmental Sciences
Jiayi Chen, Jinyu Qiu, Chuangye Yang, Yongshan Liao, Maoxiao He, Robert Mkuye, Junhui Li, Yuewen Deng, Xiaodong Du
Summary: Analyses of the transcriptome and metabolome in pearl oysters exposed to short-term hypoxia revealed alterations in key genes and metabolites. A total of 209 differentially expressed genes (DEGs) were detected between the control and hypoxia groups. Enrichment analysis showed that these DEGs were enriched in GO terms related to oxidation-reduction process, ECM organization, chaperone cofactor-dependent protein refolding, and ECM-receptor interaction KEGG pathway. Moreover, 28 differential metabolites (SDMs) implicated in metabolic pathways such as phenylalanine metabolism, D-amino acid metabolism, and aminoacyl-tRNA biosynthesis were identified. The findings suggest that pearl oysters experience oxidative stress and apoptosis under short-term hypoxia, and also undergo adaptive changes in antioxidant activity, immune and biomineralization processes, protein homeostasis, and cytoskeleton organization. This study helps uncover the mechanisms by which pearl oysters respond to short-term hypoxia adaptively.
MARINE POLLUTION BULLETIN
(2023)
Article
Biochemistry & Molecular Biology
Zhe Zheng, Ruijuan Hao, Chuangye Yang, Yu Jiao, Qingheng Wang, Ronglian Huang, Yongshan Liao, Jianbo Jian, Yao Ming, Lixin Yin, Weiming He, Ziman Wang, Chuyi Li, Qi He, Kun Chen, Yuewen Deng, Xiaodong Du
Summary: Biomineralization-controlled growth of exo-/endoskeleton contributes to body growth and size diversity. This study used pearl oysters to investigate the genetic regulation of biomineralization-controlled growth and ectopic biomineralization. The insulin-like growth factor (IGF) endocrine signal and ancient single-copy transcription factors formed the regulatory network. Cross-phylum regulation of key long noncoding RNA (lncRNA) in bivalves and mammals suggests conserved genetic and epigenetic regulation. Thyroid hormone signal and apoptosis regulation affected ectopic biomineralization in pearl oysters.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Environmental Sciences
Guixiang He, Xinwei Xiong, Yalan Peng, Chuangye Yang, Yang Xu, Xiaolong Liu, Jian Liang, Fortunatus Masanja, Ke Yang, Xin Xu, Zhe Zheng, Yuewen Deng, Jonathan Y. S. Leung, Liqiang Zhao
Summary: Marine heatwaves are expected to become more severe and frequent in the future, posing a threat to marine organisms and their communities. While the adaptability of marine organisms to heatwaves has been observed, little attention has been given to their ability to cope with repeated exposure to heatwaves, which is a common occurrence in nature.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Fisheries
Zhe Xie, Jianhang Shi, Yuntian Shi, Zhihan Tu, Menghong Hu, Chuangye Yang, Yuewen Deng, Sam Dupont, Zhixiong Xu, Youji Wang
Summary: In this study, the effects of diel-cycling hypoxia and salinity change on Hong Kong oyster physiology were examined. The results showed that the oysters' metabolism gradually shifted from aerobic respiration to glycolysis under these conditions, and their gill functions were also affected. Long-term exposure to these conditions poses a risk to the health and growth of Hong Kong oysters, as well as the aquaculture and coastal ecosystem health.
Article
Environmental Sciences
Ting Wang, Chuangye Yang, Cheng Wang, Yongshan Liao, Robert Mkuye, Yuewen Deng
Summary: This paper investigates the impact of bacterial accumulation on plastic surfaces in aquaculture facilities. It found that bacterial communities in these facilities have higher richness and diversity compared to the aquatic environment. Different types of aquaculture facilities also have different bacterial communities. The plastics provide a suitable living environment for bacteria and can influence their function. Additionally, the study detected small amounts of pathogenic bacteria in these facilities, suggesting that plastics can act as vectors for potentially harmful bacteria that may affect aquaculture development.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zhe Zheng, Zhuoxin Lai, Bin Wu, Xinlin Song, Wei Zhao, Ruzhuo Zhong, Jiawei Zhang, Yongshan Liao, Chuangye Yang, Yuewen Deng, Junpu Mei, Zhen Yue, Jianbo Jian, Qingheng Wang
Summary: In this study, a high-quality chromosome-level assembly of Sipunculus nudus was obtained using HiFi reads and high-resolution chromosome conformation capture (Hi-C) data. The assembled genome was 1,427 Mb, with a contig N50 length of 29.46 Mb and scaffold N50 length of 80.87 Mb. Approximately 98% of the genome sequence was anchored to 17 chromosomes. The presence of 97.7% of the expectedly conserved genes in the genome assembly was confirmed by BUSCO assessment. This genome will serve as an important reference for studying the genetic diversity and evolution of Lophotrochozoa.
Article
Fisheries
Meiqi Dai, Yuting Zhang, Yu Jiao, Yuewen Deng, Xiaodong Du, Chuangye Yang
Summary: This study analyzed the function and regulatory role of a novel microRNA miR-63 in the immune response of pearl oysters. The results showed that the expression of this microRNA increased after pathogen stimulation and overexpression of it resulted in decreased activity of immune and antioxidant enzymes in the serum.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Salifu Ibrahim, Chuangye Yang, Chenyang Yue, Xinyu Song, Yuewen Deng, Qi Li, Wengang Lu
Summary: The Hong Kong oyster, a commercially valuable bivalve in South China, is threatened by increased salinity in estuaries during the dry season. A whole-transcriptome analysis of oyster gills revealed differentially expressed mRNAs, long non-coding RNAs, circular RNAs, and microRNAs in response to salinity stress. These RNAs were found to be involved in amino acids metabolism, microtubule movement, and immune defense. The study provides comprehensive data for understanding the salinity response mechanism in C. hongkongensis.
MARINE BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Chuangye Yang, Hailing Wu, Jiayi Chen, Yongshan Liao, Robert Mkuye, Yuewen Deng, Xiaodong Du
Summary: The frequency of hypoxia exposure for organisms in aquatic environments is increasing due to coastal eutrophication and global warming. This study conducted an integrated analysis of transcriptomics and metabolomics to investigate the effects of long-term hypoxic stress on metabolic changes in pearl oysters. The analysis revealed significant changes in gene expression and metabolite levels, indicating oxidative stress, apoptosis, immune inhibition, and neuronal excitability reduction as responses to long-term hypoxia.
MARINE ENVIRONMENTAL RESEARCH
(2023)
