Article
Immunology
Seyedeh Alia Moosavian, Maryam Hashemi, Leila Etemad, Sara Daneshmand, Zahra Salmasi
Summary: Exosomes play a crucial role in early diagnosis and various functions related to angiogenesis, immune modulation, migration, metastasis, chemotherapy resistance, and tumor progression in melanoma. Natural exosomes have the potential to be utilized as nanocarriers and cancer vaccines for more efficient cancer treatment in the era of novel bioengineering and immunotherapy approaches.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Viviane Ponath, Nathalie Hoffmann, Leonie Bergmann, Christina Mader, Bilal Alashkar Alhamwe, Christian Preusser, Elke Pogge von Strandmann
Summary: NKp30 is a powerful cytotoxicity receptor on NK cells involved in tumor cell killing and immune response regulation. Its ligands, BAG6 and B7-H6, are upregulated in virus-infected and tumor cells, interfering with NK cell activity. The study demonstrates how soluble forms of these ligands inhibit NK cell functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Magdalena Surman, Urszula Jankowska, Magdalena Wilczak, Malgorzata Przybylo
Summary: This study used shotgun nanoLC-MS/MS to obtain protein profiles of melanoma cells and exosomes. The results provide a deeper understanding of the role of exosomes in melanoma progression and suggest potential biomarkers for circulating melanoma.
Review
Oncology
Andres Rincon-Riveros, Liliana Lopez, E. Victoria Villegas, Josefa Antonia Rodriguez
Summary: Extracellular vesicles, such as exosomes, have the potential to serve as biomarkers for cancer development, impacting various aspects of tumor progression and immune regulation.
Article
Cell Biology
Boya Peng, Trinh Mai Nguyen, Migara Kavishka Jayasinghe, Chang Gao, Thach Tuan Pham, Luyen Tien Vu, Eric Yew Meng Yeo, Gracemary Yap, Lingzhi Wang, Boon Cher Goh, Wai Leong Tam, Dahai Luo, Minh T. N. Le
Summary: In this study, the researchers demonstrated the use of extracellular vesicles (EVs) from red blood cells (RBCs) as a delivery system for RIG-I agonists in cancer cells. They found that RBCEV-delivered RIG-I agonists can activate the RIG-I pathway and induce cell death in breast cancer cells. Furthermore, targeted delivery of RIG-I agonists using RBCEVs shows potential for immunotherapy against cancer and cancer metastasis.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Article
Instruments & Instrumentation
Lorena Gonzalez-Melero, Rosa Maria Hernandez, Edorta Santos-Vizcaino, Manoli Igartua
Summary: The interest in extracellular vesicles (EVs) for cancer immunotherapy is growing. EVs are lipid bilayer vesicles released by cells, and they carry molecular signatures of their parent cells. Melanoma-derived EVs contain antigens specific to this aggressive cancer, but they also have immunomodulatory and pro-metastatic activity. Most existing reviews focus on the immune evasion characteristics of tumor-derived EVs and do not address how to overcome the associated issues. In this review, the isolation methods of EVs from melanoma patients are described, along with interesting markers to monitor their effects as antigen carriers. The methods developed so far to enhance the immunogenicity of melanoma-derived EVs, such as EV modification or adjuvant co-administration, are also discussed. In conclusion, once the optimization of EV obtaining and the understanding of the mechanisms behind their multiple effects are further advanced, EVs can be an interesting source of antigens for the development of immunotherapy.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Chenggong Tu, Zhimin Du, Hui Zhang, Yueyuan Feng, Yujun Qi, Yongjiang Zheng, Jinbao Liu, Jinheng Wang
Summary: Extracellular vesicles derived from bone marrow stromal cells can promote the cycling of multiple myeloma cells and reduce their sensitivity to bortezomib. Inhibiting endocytosis can decrease vesicle uptake by cells and enhance the anti-myeloma effects of bortezomib.
Review
Oncology
Evelyn Lattmann, Mitchell P. Levesque
Summary: Melanoma, the deadliest form of skin cancer, can be diagnosed and treated using extracellular vesicles as biomarkers, which also reveal important signaling pathways for cancer progression.
Review
Biochemistry & Molecular Biology
Maria Jose Moya-Guzman, Javiera de Solminihac, Cristina Padilla, Carolina Rojas, Camila Pinto, Tomas Himmel, Karina Pino-Lagos
Summary: This article reviews the release of small EVs by different types of leukocytes and their implications on cellular homeostasis. The functions of immune cell-derived sEVs and their potential applications in pathological conditions are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hanzhuang Liu, Shaorui Liu, Yu Xiao, Wenting Song, Huize Li, Lok Wai Cola Ho, Zhen Shen, Chung Hang Jonathan Choi
Summary: This study introduces a pH-reversible boron dipyrromethene (BODIPY) fluorescent probe for confocal imaging of extracellular vesicles (EVs) secreted from living cells. The probe allows visualization of acidic EVs in weakly basic culture medium and intracellular acidic precursor EVs in weakly basic cytoplasm, minimizing false positive signals. Additionally, the joint application of this probe with plasmid transfection reveals the secretion of some EVs from cellular pseudopodia via microtubule trackways, providing mechanistic insights into EV transport.
Review
Cell Biology
Filippo Del Vecchio, Verena Martinez-Rodriguez, Monique Schukking, Alexander Cocks, Elisabetta Broseghini, Muller Fabbri
Summary: This article discusses the interaction between extracellular vesicles and cancer cells, as well as the immune cells, especially the potential therapeutic effects of EVs released by NK cells and CTLs. It also identifies areas for further research in the field of EV-mediated immunotherapy for cancer.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Chemistry, Multidisciplinary
Silvia Fischer, Elisabeth Deindl
Summary: Extracellular vesicles (EVs) are important for cell-to-cell communication by delivering various RNA, proteins, lipids, and nucleotides. Different types of EVs, including exosomes, cell surface-derived EVs, and apoptotic bodies, contain different RNA species and can be isolated, quantified, and characterized using specific methods.
APPLIED SCIENCES-BASEL
(2021)
Review
Pharmacology & Pharmacy
Jafar Rezaie, Vahid Nejati, Monireh Mahmoodi, Mahdi Ahmadi
Summary: Extracellular vesicles (EVs), particularly MSCs-EVs, have shown great potential as a drug delivery system in nanomedicine. They can selectively deliver therapeutic agents to target cells and improve various diseases. However, there are still challenges that need to be addressed for the clinical translation of modified MSCs-EVs.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Fatih Inci
Summary: Isolating nanoparticles from complex fluids is crucial in various fields, and a microfluidic device with integrated filters has been developed to achieve multiple uses for separating exosomes from cell culture media.
Article
Hematology
Jorge Garcia Borrega, Jan-Michel Heger, Philipp Koehler, Udo Holtick, Michael Hallek, Christof Scheid, Boris Boell, Alexander Shimabukuro-Vornhagen, Matthias Kochanek, Dennis A. Eichenauer
Summary: Improvements in prognosis have been observed for allogeneic stem cell transplant recipients admitted to the ICU over the last few decades. However, patients requiring mechanical ventilation, renal replacement therapy, or vasopressors during their ICU stay showed decreased survival rates. Characteristics at baseline did not impact outcomes, and none of the patients who underwent CPR were alive at one year post-ICU discharge.
ANNALS OF HEMATOLOGY
(2022)
Article
Hematology
Petra Langerbeins, Can Zhang, Sandra Robrecht, Paula Cramer, Moritz Fuerstenau, Othman Al-Sawaf, Julia von Tresckow, Anna-Maria Fink, Karl-Anton Kreuzer, Ursula Vehling-Kaiser, Eugen Tausch, Lothar Mueller, Michael Josef Eckart, Rudolf Schlag, Werner Freier, Tobias Gaska, Christina Balser, Marcel Reiser, Martina Stauch, Clemens-Martin Wendtner, Kirsten Fischer, Stephan Stilgenbauer, Barbara Eichhorst, Michael Hallek
Summary: Observation is the standard of care for early-stage asymptomatic chronic lymphocytic leukemia (CLL) patients, as chemotherapy-based interventions have not been effective in prolonging survival. However, early intervention with ibrutinib may lead to superior disease control and is well tolerated. The CLL12 trial showed that ibrutinib significantly improved event-free survival in asymptomatic, treatment-naive early-stage CLL patients at increased risk of progression, without increasing overall toxicity.
Editorial Material
Medicine, General & Internal
M. Augustin, M. Hallek, S. Nitschmann
Editorial Material
Medicine, General & Internal
M. Augustin, M. Hallek, S. Nitschmann
Article
Oncology
Theophilos Tzaridis, Johannes Weller, Daniel Bachurski, Farhad Shakeri, Christina Schaub, Peter Hau, Andreas Buness, Uwe Schlegel, Joachim-Peter Steinbach, Clemens Seidel, Roland Goldbrunner, Niklas Schaefer, Robert J. Wechsler-Reya, Michael Hallek, Bjoern Scheffler, Martin Glas, Lothar Haeberle, Ulrich Herrlinger, Christoph Coch, Katrin S. Reiners, Gunther Hartmann
Summary: Evaluation of serum-derived extracellular vesicles (EVs) from glioblastoma patients reveals that the upregulation of CD29, CD44, CD81, C1QA, and histone H3 in serum EVs correlates with tumor progression, suggesting their potential as biomarkers.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Volker Arndt, Daniela Doege, Stefan Froehling, Peter Albers, Hana Alguel, Ralf Bargou, Carsten Bokemeyer, Martin Bornhaeuser, Christian H. Brandts, Peter Brossart, Sara Yvonne Brucker, Tim H. Bruemmendorf, Hartmut Doehner, Norbert Gattermann, Michael Hallek, Volker Heinemann, Ulrich Keilholz, Thomas Kindler, Cornelia von Levetzow, Florian Lordick, Ulf Peter Neumann, Christoph Peters, Dirk Schadendorf, Stephan Stilgenbauer, Thomas Zander, Daniel Zips, Delia Braun, Thomas Seufferlein, Gerd Nettekoven, Michael Baumann
Summary: The capacities of German Comprehensive Cancer Centers (CCCs) in various aspects of oncology care were significantly affected during the first 2 years of the COVID-19 pandemic. Follow-up, psycho-oncologic care, and tumor surgery were particularly impacted, along with other areas of multidisciplinary oncological care. This study highlights the importance of developing strategies to prevent similar limitations in the future.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Sven Borchmann, Carolin Selenz, Mia Lohmann, Hanna Ludwig, Asmae Gassa, Johannes Braegelmann, Philipp Lohneis, Lydia Meder, Julia Mattlener, Sara Breid, Marieke Nill, Jana Fassunke, Amy J. Wisdom, Anik Compes, Birgit Gathof, Hakan Alakus, David Kirsch, Khosro Hekmat, Reinhard Buettner, H. Christian Reinhardt, Michael Hallek, Roland T. Ullrich
Summary: The research shows that the novel combination immunotherapy named TRI-IT can effectively enhance the efficacy of immunotherapy in poorly immunogenic tumors by coactivating various antitumor immune responses without inducing off-target toxicity.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Anke Schilhabel, Peter Jonas Walter, Paula Cramer, Julia von Tresckow, Saskia Kohlscheen, Monika Szczepanowski, Anna Laqua, Kirsten Fischer, Barbara Eichhorst, Sebastian Boettcher, Christof Schneider, Eugen Tausch, Monika Brueggemann, Michael Kneba, Michael Hallek, Matthias Ritgen
Summary: This study investigated the impact of CD20 expression on the response to anti-CD20 therapy in chronic lymphocytic leukemia patients. A bead-based normalization approach was used to analyze CD20 expression levels, and it was found that high pre-therapeutic CD20 expression correlated with a profound response to Ofatumumab.
Article
Hematology
Jorge Garcia Borrega, Boris Boell, Matthias Kochanek, Jan-Hendrik Naendrup, Florian Simon, Noelle Sieg, Michael Hallek, Peter Borchmann, Udo Holtick, Alexander Shimabukuro-Vornhagen, Dennis A. Eichenauer, Jan-Michel Heger
Summary: A retrospective analysis was conducted on patients admitted to the intensive care unit (ICU) during hospitalization for high-dose chemotherapy and autologous stem cell transplantation (ASCT). The study found that the overall outcome was favorable for these patients.
ANNALS OF HEMATOLOGY
(2023)
Article
Oncology
Nadine Kutsch, Emily Eva Holmes, Sandra Robrecht, Gudrun Schueler, Ursula Vehling-Kaiser, Thomas Decker, Sigrun Mueller-Hagen, Karin Heinisch, Sebastian Boettcher, Matthias Ritgen, Karl-Anton Kreuzer, Stephan Stilgenbauer, Anna Maria Fink, Kirsten Fischer, Barbara Eichhorst, Michael Hallek, Clemens-Martin Wendtner
LEUKEMIA & LYMPHOMA
(2023)
Article
Cell Biology
Alexandra Brahmer, Carsten Geiss, Andriani Lygeraki, Elmo Neuberger, Theophilos Tzaridis, Tinh Thi Nguyen, Felix Luessi, Anne Regnier-Vigouroux, Gunther Hartmann, Perikles Simon, Kristina Endres, Stefan Bittner, Katrin S. Reiners, Eva-Maria Kraemer-Albers
Summary: Extracellular vesicles (EVs) released by cells carry specific biomolecules that can be used as liquid-biopsy markers for diagnosing brain diseases. This study evaluated a flow cytometry platform called EV Neuro assay for identifying and characterizing CNS-derived EVs and disease-specific markers in complex samples. The study compared different sample materials and EV isolation methods and found distinct EV profiles in glioblastoma cell lines and human astrocytes. The intensity of EV markers increased with higher EV input. Analysis of blood samples from patients with brain diseases and healthy controls showed varying EV marker intensities between individuals. Data normalization improved the identification of disease-specific markers. The EV Neuro assay proved useful for profiling EVs in complex samples. However, larger sample cohorts and higher effect sizes are needed for more reliable results in biomarker studies. Nonetheless, this study confirmed the potential of the EV Neuro assay for discovering disease-associated EV markers and monitoring circulating EV profiles in CNS diseases.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Oncology
Ruth Fluemann, Julia Hansen, Benedikt W. Pelzer, Pascal Nieper, Tim Lohmann, Ilmars Kisis, Tobias Riet, Viktoria Kohlhas, Phuong-Hien Nguyen, Martin Peifer, Nima Abedpour, Graziella Bosco, Roman K. Thomas, Moritz Kochanek, Jacqueline Knufer, Lorenz Jonigkeit, Filippo Beleggia, Alessandra Holzem, Reinhard Buttner, Philipp Lohneis, Jorn Meinel, Monika Ortmann, Thorsten Persigehl, Michael Hallek, Dinis Pedro Calado, Markus Chmielewski, Sebastian Klein, Joachim R. Goethert, Bjoern Chapuy, Branko Zevnik, F. Thomas Wunderlich, Bastian von Tresckow, Ron D. Jachimowicz, Ari M. Melnick, Hans Christian Reinhardt, Gero Knittel
Summary: Genomic profiling identified 5 subtypes of DLBCL, including the MCD/C5 cluster with MYD88, BCL2, PRDM1, and/or SPIB aberrations. Mouse models with B cell-specific Prdm1 or Spib aberrations were generated, showing molecular features of prememory and light-zone B cells. Combined BTK/BCL2 inhibition exhibited therapeutic activity in both mouse models and relapsed/refractory DLBCL patients.
BLOOD CANCER DISCOVERY
(2023)
Article
Medicine, General & Internal
L. Tometten, J. J. Malin, E. Pracht, P. J. Broeckelmann, C. Horn, R. Sprute, C. A. Langhorst, M. Hallek, G. Faetkenheuer, J. Rybniker
Summary: Immunodeficient patients with chronic and recurrent SARS-CoV-2 infections pose a significant health threat and present a major challenge in terms of treatment during the ongoing pandemic. Limited therapeutic options and emerging virus variants with immune escape mechanisms further complicate the medical care of these patients.
Meeting Abstract
Hematology
Jan-Michel Heger, Julia Mattlener, Philipp Godel, Hyatt Balke-Want, Noelle Sieg, Nadine Kutsch, Kerstin Becker, Jonathan Weiss, H. Christian Reinhardt, Michael Hallek, Peter Borchmann, Bastian von Tresckow, Sven Borchmann
Article
Hematology
Uta Gehlsen, Michael E. Stern, Jeremy Franklin, Volkan Tahmaz, Michael Hallek, Udo Holtick, Christof Scheid, Philipp Steven
Summary: Desiccating stress is identified as an independent risk factor for ocular graft-versus-host disease (oGVHD), and adjusting air humidity during transplantation has the potential to serve as a preventive measure.
TRANSPLANTATION AND CELLULAR THERAPY
(2022)