期刊
PEERJ
卷 4, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.2055
关键词
Bacteriophage; Genome; Assembly; Sequencing; Illumina
资金
- Chancellors International Scholarships from the University of Warwick
- Medical Research Council [MR/L015080/1] Funding Source: researchfish
- MRC [MR/L015080/1] Funding Source: UKRI
Bacteriophages are the most abundant biological entities on the planet, playing crucial roles in the shaping of bacterial populations. Phages have smaller genomes than their bacterial hosts, yet there are currently fewer fully sequenced phage than bacterial genomes. We assessed the suitability of Illumina technology for high-throughput sequencing and subsequent assembly of phage genomes. In silico datasets reveal that 30x coverage is sufficient to correctly assemble the complete genome of similar to 98.5% of known phages, with experimental data confirming that the majority of phage genomes can be assembled at 30x coverage. Furthermore, in silico data demonstrate it is possible to co-sequence multiple phages from different hosts, without introducing assembly errors.
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