期刊
PEERJ
卷 4, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.2316
关键词
Nasonia; Symbiosis; Microbiota; Microbiome; Axenic; Development; Speciation
资金
- NSF Awards [DEB 1046149, IOS 1456778]
- Division Of Environmental Biology
- Direct For Biological Sciences [1046149] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1456778] Funding Source: National Science Foundation
Development of a Nasonia in vitro germ-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis. However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of Nasonia. Here, we optimize the germ-free rearing procedure by using filter sterilization in lieu of antibiotics and by removing residual bleach and fetal bovine serum. Comparison of these methods reveals no influence on larval survival or growth, and a 52% improvement in adult production. Additionally, adult males produced in the new germ-free system are similar in size to conventionally reared males. Experimental implications of these changes are discussed.
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