Article
Biochemistry & Molecular Biology
Erik Schilling, Lukas Pfeiffer, Sunna Hauschildt, Ulrike Koehl, Claudia Claus
Summary: Macrophages play an important role in the pathogenesis of rubella virus (RuV) and provide insights into its interaction with the innate immune system. A study found that downregulation of CD14 expression in infected macrophages contributes to virus-host adaptation by reducing the IFN response.
Article
Environmental Sciences
Ruijia Zhang, Baowei Chen, Li Lin, Hui Zhang, Tiangang Luan
Summary: The study found that perfluorooctanoic acid (PFOA) primarily affects glucose metabolism by altering the tricarboxylic acid (TCA) cycle rather than glycolysis, leading to impaired mitochondrial respiratory function. Metabolic flux analysis (MFA) is a promising tool for investigating the toxicity mechanisms of contaminants associated with glucose metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Ophthalmology
Evan D. Bogdan, Whitney L. Stuard, Rossella Titone, Danielle M. Robertson
Summary: The study demonstrates that IGFBP-3 plays a critical role in the corneal epithelium's response to hyperosmolar stress, and supports a new function for IGFBP-3 in regulating cellular metabolism.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biology
Cristina Faccani, Gianluca Rotta, Francesca Clemente, Maya Fedeli, Danilo Abbati, Francesco Manfredi, Alessia Potenza, Achille Anselmo, Federica Pedica, Guido Fiorentini, Chiara Villa, Maria P. Protti, Claudio Doglioni, Luca Aldrighetti, Chiara Bonini, Giulia Casorati, Paolo Dellabona, Claudia de Lalla
Summary: This study describes a workflow for high-dimensional flow cytometry analysis of T cells infiltrating liver metastatic tumor lesions. By optimizing the flow cytometer settings, establishing a multi-color antibody panel, and accurately tuning the dissociation of samples, the phenotypic complexity of T cells infiltrating the tumor lesions was effectively captured. This study provides a robust tool for analyzing complex T cell populations and can be adapted to characterize other relevant pathological tissues.
LIFE SCIENCE ALLIANCE
(2022)
Article
Immunology
Frances S. Grudzinska, Alice Jasper, Elizabeth Sapey, David R. Thickett, Claudio Mauro, Aaron Scott, Jonathan Barlow
Summary: Neutrophil responses are crucial in inflammatory and infective events, but their dysregulation is linked to poor patient outcomes. Immunometabolism has provided insights into cellular functions in health and disease. This study presents optimized protocols for using XF analysis to probe glycolysis and oxidative burst in neutrophils and highlights the challenges of examining mitochondrial function using this technology. XF technology is a powerful platform with a user-friendly interface, but caution is advised when assessing neutrophil mitochondrial respiration.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Peng Zhang, Yuwen Su, Siying Li, Hui Chen, Ruifang Wu, Haijing Wu
Summary: This paper reviews the different subtypes of T lymphocytes and their functions in psoriasis, as well as provides an overview of targeted therapies for treating psoriasis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kang Nian Yap, Hoi Shan Wong, Chidambaram Ramanathan, Cristina Aurora Rodriguez-Wagner, Michael D. Roberts, David A. Freeman, Rochelle Buffenstein, Yufeng Zhang
Summary: Naked mole-rats and Damaraland mole-rats, with their small body size, exhibit extraordinary longevity, high tolerance to hypoxia and oxidative stress, and high reproductive output. Their unique bioenergetic properties, including low basal metabolic rates, a high dependence on glycolysis for ATP production, and low proton conductance across the mitochondrial inner membrane, may be an evolutionary adaptation to variable oxygen environments and a molecular mechanism underlying their extremely long lifespans.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Endocrinology & Metabolism
Abhinav Achreja, Tao Yu, Anjali Mittal, Srinadh Choppara, Olamide Animasahun, Minal Nenwani, Fulei Wuchu, Noah Meurs, Aradhana Mohan, Jin Heon Jeon, Itisam Sarangi, Anusha Jayaraman, Sarah Owen, Reva Kulkarni, Michele Cusato, Frank Weinberg, Hye Kyong Kweon, Chitra Subramanian, Max S. Wicha, Sofia D. Merajver, Sunitha Nagrath, Kathleen R. Cho, Analisa DiFeo, Xiongbin Lu, Deepak Nagrath
Summary: In this study, the researchers develop a framework called CLIM to identify collateral lethal genes using metabolic fluxes, and they use it to discover MTHFD2 as a collateral lethal gene in UQCR11-deleted ovarian tumors. They demonstrate that MTHFD2 plays a non-canonical oxidative role by providing mitochondrial NAD(+) and show the regulation of systemic metabolic activity by the paralogue metabolic pathway. The researchers also confirm the UQCR11-MTHFD2 collateral lethality in vivo, with complete remission of UQCR11-deleted ovarian tumors upon MTHFD2 inhibition.
Article
Immunology
Joseph W. George, Jane E. Mattingly, Nashanthea J. Roland, Cassandra M. Small, Benjamin G. Lamberty, Howard S. Fox, Kelly L. Stauch
Summary: The study found that DTG and FTC had different effects on mitochondrial function in HeLa and BV2 cells, suggesting that cell type-specific metabolic changes may contribute to the toxic side effects of these ARV drugs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Neurosciences
Marisa A. Jeffries, Lauren E. McLane, Luipa Khandker, Marie L. Mather, Angelina V. Evangelou, Divyangi Kantak, Jennifer N. Bourne, Wendy B. Macklin, Teresa L. Wood
Summary: This study investigated the role of mTOR signaling in oligodendrocyte lineage during remyelination. The results showed that mTOR is required for metabolic function in cuprizone-demyelinated adult brain, influencing myelin production efficiency and myelin thickness during the repair process. Loss of mTOR in OPCs did not affect remyelination efficiency after other types of lesions, suggesting a specific function in cuprizone-induced demyelination.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Plant Sciences
Kenneth Kin Leung Kwan, Huang Yun, Tina Ting Xia Dong, Karl Wah Keung Tsim
Summary: The study found that ginsenoside Rg(1) has the strongest antioxidant properties, significantly improving the performance of PC12 cells under AB oxidative stress, including increasing ATP production, enhancing spare capacity and maximal respiration, while regulating mitochondrial dynamics.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Chemistry, Medicinal
Chengchen Duan, Anna Evison, Lucy Taylor, Simone Onur, Karl Morten, Helen Townley
Summary: Citral and metformin have anti-tumor effects on RMS cells, but they work antagonistically when used together. Metformin may suppress the ROS induced by citral, leading to a reduction in its activity.
PHYTOTHERAPY RESEARCH
(2021)
Editorial Material
Cell Biology
Andres Caicedo, Keshav K. Singh
Summary: This article provides an update on the needs and highlights of the artificial mitochondrial transfer/transplant (AMT/T) field, focusing on the clinical application of AMT and the challenges that need to be addressed. Scientists have developed innovative therapeutic applications based on AMT/T, inspired by the natural transfer of mitochondria between cells. However, key questions regarding the best isolation protocol and tissue or cell source for isolation remain to be answered in order to advance the field.
Article
Biology
Jiang Zhou He, Sonia Dorion, Laura Michell Carmona-Rojas, Jean Rivoal
Summary: Nutritional phosphate deficiency has limited impact on metabolic fluxes in potato cell cultures, with sugar uptake and anaplerotic flux displaying distinct patterns over culture time.
Review
Oncology
Andres Rincon-Riveros, Liliana Lopez, E. Victoria Villegas, Josefa Antonia Rodriguez
Summary: Extracellular vesicles, such as exosomes, have the potential to serve as biomarkers for cancer development, impacting various aspects of tumor progression and immune regulation.
Article
Biochemistry & Molecular Biology
Janet R. Manning, Dharendra Thapa, Manling Zhang, Michael W. Stoner, Javier Traba, Catherine Corey, Sruti Shiva, Michael N. Sack, Iain Scott
BIOCHEMICAL JOURNAL
(2019)
Article
Cardiac & Cardiovascular Systems
Janet R. Manning, Dharendra Thapa, Manling Zhang, Michael W. Stoner, Javier Traba, Charles F. McTiernan, Catherine Corey, Sruti Shiva, Michael N. Sack, Iain Scott
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2019)
Article
Multidisciplinary Sciences
Olga M. Anton, Mary E. Peterson, Michael J. Hollander, David W. Dorward, Gunjan Arora, Javier Traba, Sumati Rajagopalan, Erik L. Snapp, K. Christopher Garcia, Thomas A. Waldmann, Eric O. Long
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Munir Akkaya, Abhisheka Bansal, Patrick W. Sheehan, Mirna Pena, Alvaro Molina-Cruz, Lindsey M. Orchard, Clare K. Cimperman, Chen-Feng Qi, Philipp Ross, Takele Yazew, Daniel Sturdevant, Sarah L. Anzick, Girija Thiruvengadam, Thomas Dan Otto, Oliver Billker, Manuel Llinas, Louis H. Miller, Susan K. Pierce
Article
Multidisciplinary Sciences
Javier Traba, Thomas A. Waldmann, Olga M. Anton
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Cell Biology
Billur Akkaya, Ethan M. Shevach
CELLULAR IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Sarah S. Geiger, Javier Traba, Nathan Richoz, Taylor K. Farley, Stephen R. Brooks, Franziska Petermann, Lingdi Wang, Frank J. Gonzalez, Michael N. Sack, Richard M. Siegel
Summary: The time of day influences immune responses and lethality in response to LPS, with the highest survival rate at the beginning of the light cycle. Feeding, rather than light, is shown to control time-of-day dependent LPS sensitivity through liver clock and hepatic FXR signaling.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Javier Traba, Michael N. Sack, Thomas A. Waldmann, Olga M. Anton
Summary: This article discusses the main effects of the tumor microenvironment (TME) on the metabolism and function of immune cells, and reviews emerging strategies to boost immune cell metabolism for promoting anti-tumor effects, either as monotherapies or in combination with conventional chemotherapy for optimizing cancer treatment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Daisuke Araki, Jian Fei Fu, Heather Huntsman, Stefan Cordes, Fayaz Seifuddin, Luigi J. Alvarado, Patali S. Cheruku, Ayla Cash, Javier Traba, Yuesheng Li, Mehdi Pirooznia, Richard H. Smith, Andre Larochelle
Summary: Activation of NOTCH signaling using engineered DELTA-like ligand (DXI) under low oxygen tension culture conditions in human adult HSPCs can limit ER stress, increase the frequency of LTR-HSCs significantly, and help maintain undifferentiated phenotypes in cultured cells.
Letter
Pediatrics
Sigmund J. J. Kharasch, Pietro Miozzo, Teresa Victoria, Helene Dumas, Sivan Avramovich, Onyinyechi Eke, Virginia Kharasch
PEDIATRIC PULMONOLOGY
(2023)
Article
Cell Biology
Kim Han, Komudi Singh, Allison M. Meadows, Rahul Sharma, Shahin Hassanzadeh, Jing Wu, Haley Goss-Holmes, Rebecca D. Huffstutler, Heather L. Teague, Nehal N. Mehta, Julian L. Griffin, Rong Tian, Javier Traba, Michael N. Sack
Summary: NAD+ boosting can modulate adaptive immunity by reducing IFNg and IL-17 secretion, enhancing Th17 polarization, and reducing inflammation in psoriasis through activation of Nrf2 and increased antioxidant defenses.
CELL REPORTS MEDICINE
(2023)
Article
Physiology
Aurea Oliva, Carolina Merono, Javier Traba
Summary: Mitochondria have a crucial role in the activation of the immune system, regulating cell metabolism and immune pathways. Dysregulation of mitochondria can lead to human diseases.
CURRENT OPINION IN PHYSIOLOGY
(2022)
Review
Immunology
Munir Akkaya, Kihyuck Kwak, Susan K. Pierce
NATURE REVIEWS IMMUNOLOGY
(2020)
Meeting Abstract
Immunology
Billur Akkaya, Munir Akkaya, Jafar A. Al Souz, Amanda H. Holstein, Mitra Maz, Juraj Kabat, Olena Kamenyeva, Ethan M. Shevach
JOURNAL OF IMMUNOLOGY
(2019)
Meeting Abstract
Immunology
Billur Akkaya, Yoshihiro Oya, Munir Akkaya, Jafar A. Al Souz, Amanda H. Holstein, Juraj Kabat, Olena Kamenyeva, Dave Dorward, Deborah Glass, Ethan M. Shevach
JOURNAL OF IMMUNOLOGY
(2019)