期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 4, 期 3, 页码 547-556出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb01964h
关键词
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资金
- National Natural Science Foundation of China [31271019, 51573071]
- Natural Science Foundation of Guangdong Province [2014A030313361]
- Pearl River S&T Nova Program of Guangzhou [201506010069]
For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N, N'-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine) s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.
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