Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET

(2015) John W. Hardin et al.   NUCLEIC ACIDS RESEARCH

The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing

(2015) Rogerio Alves de Almeida et al.   RNA Biology

Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5′ splice site

(2015) Carsten Boesler et al.   RNA

Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull

(2015) Margaret L. Rodgers et al.   RNA

Spliceosome assembly in the absence of stable U4/U6 RNA pairing

(2015) Jordan E. Burke et al.   RNA

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome

(2014) A. M. Wlodaver et al.   RNA

Visualizing cellular machines with colocalization single molecule microscopy

(2013) Joshua D. Larson et al.   CHEMICAL SOCIETY REVIEWS

Sad1 Counteracts Brr2-Mediated Dissociation of U4/U6.U5 in Tri-snRNP Homeostasis

(2013) Y.-H. Huang et al.   MOLECULAR AND CELLULAR BIOLOGY

HnRNP L and HnRNP A1 Induce Extended U1 snRNA Interactions with an Exon to Repress Spliceosome Assembly

(2013) Ni-Ting Chiou et al.   MOLECULAR CELL

Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing

(2013) Ramya Krishnan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Splicing proofreading at 5′ splice sites by ATPase Prp28p

(2013) Fei Yang et al.   NUCLEIC ACIDS RESEARCH

Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site

(2013) D. J. Crawford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complex

(2013) K. S. Karunatilaka et al.   RNA

Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy

(2013) Inna Shcherbakova et al.   Cell Reports

Kinetic Schemes for Post-Synchronized Single Molecule Dynamics

(2012) Chunlai Chen et al.   BIOPHYSICAL JOURNAL

Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre

(2012) Nicolas Rasche et al.   EMBO JOURNAL

The Interaction of Prp2 with a Defined Region of the Intron Is Required for the First Splicing Reaction

(2012) H.-L. Liu et al.   MOLECULAR AND CELLULAR BIOLOGY

The spliceosome: a flexible, reversible macromolecular machine

(2012) Aaron A. Hoskins et al.   TRENDS IN BIOCHEMICAL SCIENCES

Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging

(2011) Xiaoli Sun et al.   CHEMBIOCHEM

Ordered and Dynamic Assembly of Single Spliceosomes

(2011) A. A. Hoskins et al.   SCIENCE

The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing

(2010) Rebecca Hogg et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses

(2010) Dmitry Cherny et al.   EMBO JOURNAL

The DEAH Box ATPases Prp16 and Prp43 Cooperate to Proofread 5′ Splice Site Cleavage during Pre-mRNA Splicing

(2010) Prakash Koodathingal et al.   MOLECULAR CELL

Conformational dynamics of single pre-mRNA molecules during in vitro splicing

(2010) John Abelson et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing

(2010) R. M. Lardelli et al.   RNA

The Spliceosome: Design Principles of a Dynamic RNP Machine

(2009) Markus C. Wahl et al.   CELL

The Evolutionarily Conserved Core Design of the Catalytic Activation Step of the Yeast Spliceosome

(2009) Patrizia Fabrizio et al.   MOLECULAR CELL

Single-molecule analysis of protein-free U2–U6 snRNAs

(2009) Zhuojun Guo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Do-it-yourself guide: how to use the modern single-molecule toolkit

(2008) Nils G Walter et al.   NATURE METHODS

A role for ubiquitin in the spliceosome assembly pathway

(2008) Priya Bellare et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Both Catalytic Steps of Nuclear Pre-mRNA Splicing Are Reversible

(2008) C.-K. Tseng et al.   SCIENCE