Article
Cell & Tissue Engineering
Xing Zhang, Yihong Jiang, Qun Huang, Zhaoyu Wu, Hongji Pu, Zhijue Xu, Bo Li, Xinwu Lu, Xinrui Yang, Jinbao Qin, Zhiyou Peng
Summary: Exosomes secreted by ADSCs overexpressing GLO-1 promoted endothelial cell protection and angiogenesis in diabetic mice with limb ischemia, showing promising potential for clinical treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Peripheral Vascular Disease
Yuuki Shimizu, Kazuhisa Kondo, Ryo Hayashida, Ken-ichiro Sasaki, Masanori Ohtsuka, Yoshihiro Fukumoto, Shinichiro Takashima, Oto Inoue, Soichiro Usui, Masayuki Takamura, Masashi Sakuma, Teruo Inoue, Tokuichiro Nagata, Yoshihiro J. Akashi, Yoshihiro Yamada, Tamon Kato, Koichiro Kuwahara, Kaoru Tateno, Yoshio Kobayashi, Rei Shibata, Toyoaki Murohara
Summary: This multicenter clinical trial demonstrated that autologous adipose-derived regenerative cell (ADRC) transplantation is safe and effective for therapeutic angiogenesis in patients with critical limb ischemia (CLI). The overall survival rate was 100% at the post-operative period and 6 months follow-up. 94.1% of limbs were free from major amputation for 6 months. The quality of life score, ulcer size, and walking distance also improved in a significant number of patients.
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Peripheral Vascular Disease
Seyedeh-Leili Asadi-Yousefabad, Pegah Nammian, Seyed Mohammad Bagher Tabei, Sajad Daneshi, Jafar Fallahi, Vahid Razban, Mohammad Hasan Sheikhha
Summary: This study aimed to enhance the therapeutic potential of AT-MSCs by overexpressing miR-126 and provide an effective treatment for angiogenesis in CLI. The results demonstrated that the miR-126 treated group showed better outcomes in function scores, VEGF-A levels, and histopathology compared to the control group. Furthermore, miR-126 downregulated the expression of PIK3R2 and SPRED1 and prolonged the survival of MSCs in the muscles.
MICROVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Tao Bai, Mingxing Li, Yuanfeng Liu, Zhentao Qiao, Xusheng Zhang, Yafeng Wang, Zhiwei Wang
Summary: The study found that AURKA plays an important role in diabetes-related ischemic limb ischemia, improving vascular regeneration and functional recovery. In addition, AURKA can also inhibit oxidative stress and lipid peroxidation reactions, providing protection against diabetic limb ischemia.
MOLECULAR MEDICINE
(2023)
Article
Cell & Tissue Engineering
Christy Wing Tung Wong, Apurva Sawhney, Yalan Wu, Yi Wah Mak, Xiao Yu Tian, Hon Fai Chan, Anna Blocki
Summary: This study demonstrated that functional BDACs can be sourced under xeno- and/or serum-free conditions and showed superior therapeutic effects compared to traditional MSCs in a murine model of CLI. The secretome of BDACs contained a larger number of proteins with pro-angiogenic and anti-inflammatory properties, potentially contributing to their enhanced therapeutic potential.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Engineering, Biomedical
Gyu Tae Park, Jae Kyung Lim, Eun-Bae Choi, Mi-Ju Lim, Bo-Young Yun, Dae Kyoung Kim, Jung Won Yoon, Yoon Gi Hong, Jae Hoon Chang, Seong Hwan Bae, Jung Yong Ahn, Jae Ho Kim
Summary: In this study, microvascular fragments, stromal vascular fractions, and mesenchymal stem cells were isolated from human lipoaspirate and their therapeutic efficacy and in vivo vasculogenic activity were investigated. The results showed that microvascular fragments had improved engraftment efficiency and vasculogenic activity compared to mesenchymal stem cells and stromal vascular fractions, making them highly useful for treating ischemic diseases and in tissue engineering.
BIOMATERIALS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Michiko Horiguchi, Yuko Okada, Yuya Turudome, Kentaro Ushijima
Summary: Type 1 diabetes mellitus leads to degeneration of organelles in adipose-derived stem cells, with the main characteristic being prominent enlarged spherical vesicles. The exosomes of T1DM ADSCs are found to be enlarged, reduced in number, and increased in the percentage positive for tetraspanin CD9, providing insight into the characteristics of stem cells in patients with type 1 diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Michiko Horiguchi, Yuya Tsurudome, Kentaro Ushijima
Summary: This study reveals that AMFR and DCTN2 genes reduce the transplantation efficiency of T1DM ADSCs, and decreasing their expression can improve transplant outcomes.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Marta Rojas-Torres, Ma Carmen Duran-Ruiz
Summary: Critical limb ischemia is the most severe form of peripheral arterial disease, commonly caused by atherosclerosis leading to progressive blockage of arterial vessels. For patients not suitable for surgical intervention, angiogenic cell therapy has emerged as a promising alternative, showing potential for enhancing revascularization by promoting vessel formation and blood flow recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Grazia Marsico, Chunseng Jin, Sunny A. Abbah, Eva M. Brauchle, Dilip Thomas, Ana Lucia Rebelo, Doriana Orbanic, Sandrine Chantepie, Paolo Contessotto, Dulce Papy-Garcia, Carlos Rodriguez-Cabello, Michelle Kilcoyne, K. Schenke-Layland, N. G. Karlsson, Karl J. A. McCullagh, Abhay Pandit
Summary: This study demonstrated glycosylation changes associated with ischemia in a murine CLI model, and evaluated the efficacy of an ELR hydrogel for CLI treatment. The ELR hydrogel modulated angiogenic signaling pathways, promoted capillary formation and ECM remodeling, while also inducing arterioles formation, reducing fibrosis, and polarizing anti-inflammatory macrophages. The study suggests a potential role for mannosylation and sialylation in tissue repair, highlighting glycosylation alterations as new therapeutic targets for CLI.
Review
Biochemistry & Molecular Biology
Gemma Arderiu, Anna Civit-Urgell, Lina Badimon
Summary: The incidence and prevalence of critical limb ischemia have increased, but there are no successful treatments available. Adipose-derived stem cells (ASCs) are a promising source of stem cells for cell-based therapy due to their multilineage capacity and low immunogenicity. The proangiogenic benefits of ASCs are attributed to their secretion of proangiogenic molecules, microvesicles/exosomes, and their differentiation capability toward endothelial cells (ECs). However, the therapeutic efficacy of ASCs in peripheral artery diseases patients is still relatively low.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mingming Wang, Hongyan Zhan, Jianhua Wang, Hua Song, Jianhua Sun, Gang Zhao
Summary: This study demonstrates that CS can enhance the proliferation of ADSCs and improve their migration ability through inhibiting apoptosis and oxidative stress. CS also promotes the expression of CXCR4 in ADSCs and enhances their migration and angiogenic capacity. Importantly, CS improves the efficacy of ADSCs in wound healing both in vivo and in vitro, providing potential materials for the treatment of wound healing.
Article
Chemistry, Multidisciplinary
Zhiyou Peng, Jinbao Qin, Bo Li, Kaichuang Ye, Yuxin Zhang, Xinrui Yang, Fukang Yuan, Lijia Huang, Junqing Hu, Xinwu Lu
Article
Chemistry, Multidisciplinary
Jinbao Qin, Zhiyou Peng, Bo Li, Kaichuang Ye, Yuxin Zhang, Fukang Yuan, Xinrui Yang, Lijia Huang, Junqing Hu, Xinwu Lu
Article
Cell & Tissue Engineering
Jinbao Qin, Fukang Yuan, Zhiyou Peng, Kaichuang Ye, Xinrui Yang, Lijia Huang, Mier Jiang, Xinwu Lu
STEM CELL RESEARCH & THERAPY
(2015)
Article
Surgery
K. Ye, R. Wang, J. Qin, X. Yang, M. Yin, X. Liu, M. Jiang, X. Lu
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2016)
Article
Cardiac & Cardiovascular Systems
Jinbao Qin, Zhen Zhao, Ruihua Wang, Kaichuang Ye, Weimin Li, Xiaobing Liu, Guang Liu, Chaoyi Cui, Huihua Shi, Zhiyou Peng, Fukang Yuan, Xinrui Yang, Min Lu, Xintian Huang, Mier Jiang, Xin Wang, Minyi Yin, Xinwu Lu
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2017)
Article
Surgery
Kaichuang Ye, Huihua Shi, Minyi Yin, Jinbao Qin, Xinrui Yang, Xiaobing Liu, Mier Jiang, Xinwu Lu
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2018)
Article
Nanoscience & Nanotechnology
Jinbao Qin, Chen Peng, Binghui Zhao, Kaichuang Ye, Fukang Yuan, Zhiyou Peng, Xinrui Yang, Lijia Huang, Mier Jiang, Qinghua Zhao, Guangyu Tang, Xinwu Lu
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2014)
Article
Materials Science, Multidisciplinary
Xiaoyu Wu, Kun Liu, Qun Huang, Qin Zhang, Xinrui Yang, Xiaobing Liu, Ruihua Wang
Summary: CuS nanoparticles have shown potential in alleviating arterial inflammation in post-injury remodeling of the arterial wall. The study demonstrated efficient depletion of macrophages by CuS NPs, leading to alleviation of arterial stenosis.
FRONTIERS IN MATERIALS
(2021)
Article
Surgery
Xinrui Yang, Xiaoyu Wu, Zhiyou Peng, Minyi Yin, Xinwu Lu, Kaichuang Ye
Summary: This study evaluated the clinical outcomes of treating patients with nonthrombotic iliac vein lesions (NIVLs) and venous ulcers by including iliac vein stenting in the treatment plan. Results showed that incorporating stent placements as part of the treatment for NIVLs led to higher ulcer healing rates and improved venous clinical severity scores.
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2021)
Article
Cell & Tissue Engineering
Xing Zhang, Yihong Jiang, Qun Huang, Zhaoyu Wu, Hongji Pu, Zhijue Xu, Bo Li, Xinwu Lu, Xinrui Yang, Jinbao Qin, Zhiyou Peng
Summary: Exosomes secreted by ADSCs overexpressing GLO-1 promoted endothelial cell protection and angiogenesis in diabetic mice with limb ischemia, showing promising potential for clinical treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Surgery
Xinrui Yang, Xing Zhang, Minyi Yin, Ruihua Wang, Xinwu Lu, Kaichuang Ye
Summary: The study suggests that elastic compression stockings (ECS) can prevent post-thrombotic syndrome (PTS) in patients with IFDVT and femoral-popliteal venous thrombosis who did not undergo thrombus removal procedures. The ECS group had a lower incidence of PTS at 24 months, higher quality of life scores, and better symptom relief compared to the control group.
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2022)
Article
Surgery
Kaichuang Ye, Peng Qiu, Jinbao Qin, Zhiyou Peng, Weimin Li, Minyi Yin, Xinwu Lu
Summary: This study evaluated the outcomes of internal iliac artery reconstruction using in situ laser-assisted fenestration during endovascular aortic repair for abdominal aortic aneurysm. The results showed that this technique was safe and effective, with a high technical success rate and no major adverse events within 30 days. Long-term follow-up also demonstrated good patency of the reconstructed arteries.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Chemistry, Multidisciplinary
Xing Zhang, Junchao Liu, Xinrui Yang, Guanjie He, Bo Li, Jinbao Qin, Paul R. Shearing, Dan J. L. Brett, Junqing Hu, Xinwu Lu
Article
Medicine, Research & Experimental
Xin Wang, Jinbao Qin, Xing Zhang, Zhiyou Peng, Kaichuang Ye, Xiaoyu Wu, Xinrui Yang, Huihua Shi, Zhen Zhao, Xin Guo, Xiaobing Liu, Minyi Yin, Xinwu Lu