Review
Biochemistry & Molecular Biology
Renu A. Kowluru
Summary: Diabetic retinopathy, a leading cause of vision loss in working-age adults, involves oxidative stress and the activation of Nox2 and Rac1, with inhibitors of these enzymes potentially serving as therapeutic interventions.
Article
Neurosciences
Matthew T. Keeney, Eric K. Hoffman, Kyle Farmer, Christopher R. Bodle, Marco Fazzari, Alevtina Zharikov, Sandra L. Castro, Xiaoping Hu, Amanda Mortimer, Julia K. Kofler, Eugenia Cifuentes-Pagano, Patrick J. Pagano, Edward A. Burton, Teresa G. Hastings, J. Timothy Greenamyre, Roberto Di Maio
Summary: Mitochondrial dysfunction and oxidative stress play important roles in the pathogenesis of Parkinson's disease, and mitochondrially-generated superoxide can activate NOX2. The study found that both neuronal and microglial NOX2 are highly activated in substantia nigra in chronic conditions, but only neuronal NOX2 is activated in acute and sub-acute models, suggesting its primary role in the early stages of the disease.
NEUROBIOLOGY OF DISEASE
(2022)
Review
Immunology
Hiroyuki Yoshitomi
Summary: Tph cells, characterized by CXCL13 production and efficient help to B cells, play important roles in the pathogenesis of RA and other diseases such as autoimmune diseases, infectious diseases, and cancers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hassan Dakik, Maya El Dor, Joan Leclerc, Farah Kouzi, Ali Nehme, Margaux Deynoux, Christelle Debeissat, Georges Khamis, Elfi Ducrocq, Aida Ibrik, Marie-Jose Stasia, Houssam Raad, Hamid Reza Rezvani, Fabrice Gouilleux, Kazem Zibara, Olivier Herault, Frederic Mazurier
Summary: The study found that NOX2 is widely expressed in AML blasts, especially in certain types of cells, but less expressed in stem cells and relapsed AML. Endogenous NOX activity could not be detected without stimulation. Although CYBB knockdown affected NOX2 activity, it did not impact the proliferation and differentiation of THP-1 and HL-60 cells.
Article
Immunology
Chih-Fen Hu, San-Pin Wu, Gu-Jiun Lin, Chi-Chang Shieh, Chih-Sin Hsu, Jing-Wun Chen, Shih-Heng Chen, Jau-Shyong Hong, Shyi-Jou Chen
Summary: The study showed that Nox2 deficiency attenuates neural damage induced by EAE, reduces disease severity, pathogenic immune cells infiltration, demyelination, and oxidative stress in the CNS. It also revealed that microglial Nox2 plays a regulatory role in multiple pathways important for MS/EAE pathogenesis, suggesting that Nox2 inhibition may represent an important therapeutic target for MS.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rodrigo Prieto-Bermejo, Marta Romo-Gonzalez, Alejandro Perez-Fernandez, Maria Carmen Garcia-Macias, Carmen Sanchez-Bernal, Ignacio Garcia-Tunon, Jesus Sanchez-Yague, Manuel Sanchez-Martin, Angel Hernandez-Hernandez
Summary: The study demonstrates that Cyba(-/-) mice with CGD spontaneously develop granulomas, serving as a valuable model for studying the disease. The altered homeostasis of hematopoiesis in these mice, marked by significant weight loss, splenomegaly, myeloid hyperplasia, and anemia, suggests a possible contribution to the poor outcomes in CGD patients besides their defective innate immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Shanshan Xu, Yubo Ma, Yuting Chen, Faming Pan
Summary: FOXO3a, a critical member of the Forkhead box O family, plays a significant role in cell proliferation, apoptosis, immune cell activity and cytokine expression. It is associated with the progression and chemoresistance of various human cancers, and also contributes to the pathogenesis of autoimmune diseases.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yosef Fichman, Sara Zandalinas, Scott Peck, Sheng Luan, Ron Mittler
Summary: The leucine-rich-repeat receptor-like kinase HPCA1 plays a key role in mediating and coordinating systemic cell-to-cell reactive oxygen species and calcium signals required for plant acclimation to stress.
Article
Multidisciplinary Sciences
Yao Li, Damir Kracun, Christopher M. Dustin, Mohamed El Massry, Shuai Yuan, Christian J. Goossen, Evan R. DeVallance, Sanghamitra Sahoo, Cynthia St Hilaire, Aditi U. Gurkar, Toren Finkel, Adam C. Straub, Eugenia Cifuentes-Pagano, Patrick J. Pagano
Summary: In an investigation into signaling triggered by aging and hyperglycemia, researchers found that NADPH Oxidase (NOX) plays a crucial role in driving cell damage, inflammation, and cellular senescence. Inhibiting NOX1 can reverse age-related impairments in blood flow and angiogenesis, as well as disrupt proinflammatory signaling associated with senescence. Targeting the NOX1-SASP signaling axis is predicted to be an effective strategy for mitigating vascular and organ system senescence.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Zahra Maria, Emma Turner, Agnieshka Agasing, Gaurav Kumar, Robert C. Axtell
Summary: The study showed that pertussis toxin (PTX) significantly reduced Th17-EAE by inhibiting chemokine-receptor-dependent trafficking of Th17 cells and promoting the accumulation of B cells in the central nervous system. The data suggest that PTX inhibits the migration of pathogenic Th17 cells while promoting the accumulation of pathogenic B cells during Th17-EAE, providing useful information for adoptive-transfer Th17-EAE and studying the pathogenic mechanisms of B cells in multiple sclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hannah Mason, Ganesha Rai, Arina Kozyr, Nathaniel De Jonge, Emily Gliniewicz, Lars J. Berg, Gal Wald, Cayce Dorrier, Mark J. Henderson, Alexey Zakharov, Tristan Dyson, John Audley, Anthony M. Pettinato, Elias Carvalho Padilha, Pranav Shah, Xin Xu, Thomas L. Leto, Anton Simeonov, Kol A. Zarember, Dorian B. McGavern, John I. Gallin
Summary: NADPH oxidases (NOX's) and the reactive oxygen species (ROS) they produce are involved in various physiological processes, but excessive ROS production is associated with several diseases. In this study, a small molecule inhibitor, NCATS-SM7270, was developed and shown to specifically inhibit NOX2 activity in human and mouse granulocytes. The role of different NOX isoforms in mild traumatic brain injury (mTBI) was investigated, and it was found that NOX2 deficiency provided protection against mTBI pathology, while NOX4 deficiency exacerbated the injury. Treatment of mice with NCATS-SM7270 after mTBI reduced cortical cell death in a dose-dependent manner, and also partially reversed cortical damage in NOX4-deficient mice. These findings highlight the potential of NCATS-SM7270 as a specific inhibitor of NOX2 in protecting against NOX2-dependent cell death in mTBI.
Review
Biochemistry & Molecular Biology
Qinhong Wang, Rahima Zennadi
Summary: Sickle cell disease is a genetic disorder characterized by oxidative stress in red blood cells, leading to various systemic manifestations.
Review
Immunology
Lixiang Wang, Xin Wei
Summary: Glaucoma, a leading neurodegenerative disease, has traditionally been treated by controlling intraocular pressure. Recent studies suggest autoimmune aspects may play a role in glaucoma, similar to other central nervous system diseases. Immune modulation therapy could be a potential solution for glaucoma, inspired by findings in CNS neurodegenerative diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Per-Ole Carstens, Luisa M. Muellar, Arne Wrede, Sabrina Zechel, Martin M. Wachowski, Almuth Brandis, Sabine Krause, Stephan Zierz, Jens Schmidt
Summary: This study identified higher expression of BAFF, APRIL, and CXCL-13 in IBM and PM patients compared to controls, suggesting an involvement of B cell-related mechanisms in the pathophysiology of IBM and PM. Additionally, muscle fibers may play a role in the recruitment of B cells and sustaining inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Tobias Boettler, Benedikt Csernalabics, Henrike Salie, Hendrik Luxenburger, Lara Wischer, Elahe Salimi Alizei, Katharina Zoldan, Laurenz Krimmel, Peter Bronsert, Marius Schwabenland, Marco Prinz, Carolin Mogler, Christoph Neumann-Haefelin, Robert Thimme, Maike Hofmann, Bertram Bengsch
Summary: This case report highlights the occurrence of autoimmune hepatitis episodes following COVID-19 vaccination in a patient with distinctive T cell-dominant immune-mediated hepatitis. Liver tissue analysis revealed an immune infiltrate with activated cytotoxic CD8 T cells predominantly distributed, indicating a unique pathomechanism associated with vaccination-induced antigens.
JOURNAL OF HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mark D. Stevenson, Chandrika Canugovi, Aleksandr E. Vendrov, Takayuki Hayami, Dawn E. Bowles, Karl-Heinz Krause, Nageswara R. Madamanchi, Marschall S. Runge
ANTIOXIDANTS & REDOX SIGNALING
(2019)
Article
Medicine, Research & Experimental
Francis Rousset, Patrick Salmon, Simon Bredl, Ophelie Cherpin, Marta Coelho, Renier Myburgh, Marco Alessandrini, Michael Perny, Marta Roccio, Roberto F. Speck, Pascal Senn, Karl Heinz Krause
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Editorial Material
Multidisciplinary Sciences
Sten Ilmjarv, Fiona Augsburger, Jerven Tjalling Bolleman, Robin Liechti, Alan James Bridge, Jenny Sandstrom, Vincent Jaquet, Ioannis Xenarios, Karl-Heinz Krause
Article
Clinical Neurology
Francis Rousset, Vivianne Beatrix Christina Kokje, Marta Da Costa Coelho, Thibault Mugnier, Emilie Belissa, Doris Gabriel, Robert Gurny, Karl Heinz Krause, Pascal Senn
OTOLOGY & NEUROTOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Fiona Augsburger, Aleksandra Filippova, Delphine Rasti, Tamara Seredenina, Magdalena Lam, Ghassan Maghzal, Zahia Mahiout, Pidder Jansen-Duerr, Ulla G. Knaus, James Doroshow, Roland Stocker, Karl-Heinz Krause, Vincent Jaquet
Article
Cell & Tissue Engineering
Karlien Kallmeyer, Dominik Andre-Levigne, Mathurin Baquie, Karl-Heinz Krause, Michael S. Pepper, Brigitte Pittet-Cuenod, Ali Modarressi
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Francis Rousset, German Nacher-Soler, Marta Coelho, Sten Ilmjarv, Vivianne Beatrix Christina Kokje, Antoine Marteyn, Yves Cambet, Michael Perny, Marta Roccio, Vincent Jaquet, Pascal Senn, Karl Heinz Krause
Article
Cell & Tissue Engineering
Erika Cosset, Tieng Vannary, Frederique Sloan-Bena, Stefania Gimelli, Eric Gerstel, Karl-Heinz Krause, Antoine Marteyn
STEM CELL RESEARCH
(2020)
Article
Cell Biology
Manon Locatelli, Flavien Delhaes, Ophelie Cherpin, Margaret E. Black, Stephanie Carnesecchi, Olivier Preynat-Seauve, Youssef Hibaoui, Karl-Heinz Krause
Summary: Cell therapies based on pluripotent stem cells have provided new therapeutic strategies for neurodegenerative diseases. This study suggests that the cell cycle-dependent promoter SR39h may be the most efficient and selective safety switch for neural transplants. Nucleoside analogs such as PCV and GCV can effectively induce cell death with low toxicity.
Article
Biochemistry & Molecular Biology
Nikolay Zhukovsky, Marianna Silvano, Thierry Filloux, Sergio Gonzalez, Karl-Heinz Krause
Summary: This study demonstrates that human alpha-1 antitrypsin (hAAT) may slow down the progression of Charcot-Marie-Tooth disease type 1 (CMT1A) by reducing inflammation and regulating ADAM-17. In a mouse model, hAAT significantly improved muscle activity and histopathological features, and decreased levels of the inflammatory marker IL-6.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Astrid Malezieux-Picard, Fabien Abdul, Francois R. Herrmann, Aurelie Caillon, Pascale Ribaux, Yves Cambet, Sabine Yerly, Stephanie Baggio, Nathalie Vernaz, Dina Zekry, Karl-Heinz Krause, Olivier Preynat-Seauve, Virginie Prendki
Summary: This study investigated fusion inhibitory antibodies (fiAbs) levels in COVID-19 patients and analyzed their association with frailty. The results showed that elderly patients had lower fiAbs levels, which were associated with frailty. These findings provide insights into the disease severity of elderly COVID-19 patients.
Article
Virology
Fabien Abdul, Pascale Ribaux, Aurelie Caillon, Astrid Malezieux-Picard, Virginie Prendki, Nathalie Vernaz, Nikolay Zhukovsky, Flavien Delhaes, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: This study reports a new method for measuring fusion-inhibitory antibodies in serum, with a high degree of standardization and the ability for automation.
Review
Cell Biology
Sanae El Harane, Bochra Zidi, Nadia El Harane, Karl-Heinz Krause, Thomas Matthes, Olivier Preynat-Seauve
Summary: Spheroids and organoids are emerging as important tools in medical and life science research, replacing traditional 2D cell cultures. These 3D cultures offer a more realistic representation of in vivo conditions and hold great potential for academic research, drug screening, and personalized medicine. Various methods have been developed for generating 3D structures, depending on the tissue origin, presence of disease, and intended application. This review provides an overview of methods for generating cancer spheroids and organoids, highlighting their advantages, limitations, and potential therapeutic applications.
Article
Biochemistry & Molecular Biology
Nicolo Costantino Brembilla, Ali Modarressi, Dominik Andre-Levigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stephane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). The results show that the gelatin sponge strongly influences the transcriptome of ASC and acted as a concentrator and reservoir of the regenerative ASC secretome. The gelatin sponge also increased angiogenesis in vivo and promoted wound healing.
Article
Medicine, General & Internal
M. Alessandrini, K-H Krause, R. F. Speck, M. S. Pepper
SAMJ SOUTH AFRICAN MEDICAL JOURNAL
(2019)