Article
Biophysics
Shih-Mo Yang, Qiang Lin, Hongbo Zhang, Ruixue Yin, Wenjun Zhang, Minchao Zhang, Yubao Cui
Summary: The study introduced a method utilizing electrokinetic effects to improve the efficiency of cytokine detection, resulting in an increased fill percentage of encoded beads and successful calculation of the concentration of four target cytokines to the pg/ml level. The application of DEP is expected to enhance the sensitivity of digital ELISA for rapid detection of multiple targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Cesar A. Patino, Nibir Pathak, Prithvijit Mukherjee, So Hyun Park, Gang Bao, Horacio D. Espinosa
Summary: This study presents a high-throughput localized electroporation device assisted by deep learning image analysis for efficient biomolecular delivery optimization. The platform showcases versatility in delivering biomolecules into various cell types, enabling multicargo delivery with precise control.
Article
Multidisciplinary Sciences
Yannik Severin, Benjamin D. Hale, Julien Mena, David Goslings, Beat M. Frey, Berend Snijder
Summary: Phenotypic plasticity in the immune system is influenced by donor age, gender, and blood pressure, resulting in distinct polarization and activation-associated phenotypes across immune cell classes. The morphology of T cells is associated with their transcriptional state, with inflammation-associated polarized T cell morphology and age-associated loss of mitochondria observed. This study reveals that immune cell phenotypes reflect both molecular and personal health information, providing new perspectives for deep immune phenotyping in individuals' health and disease.
Article
Chemistry, Multidisciplinary
Xinxin Hang, Shiqi He, Zaizai Dong, Yun Li, Zheng Huang, Yanruo Zhang, Hong Sun, Long Lin, Hu Li, Yang Wang, Bing Liu, Nan Wu, Tianling Ren, Yubo Fan, Jizhong Lou, Ruiguo Yang, Lan Jiang, Lingqian Chang
Summary: This study introduces a DNA tensioner platform with high throughput and pN-level resolution to detect the mechanical forces of individual cells. Using this platform, enhanced mechanical forces in drug-resistant cells and metastatic tumor cells, as well as the mechanical heterogeneity associated with genes VEGFA and MINK1, were identified. The platform has the potential to establish links between cellular mechanical heterogeneity and genetic heterogeneity.
Article
Optics
Kevin Byard
Summary: A method is described to create contiguous coded aperture patterns with approximately 50% throughput by mosaicing linear uniformly redundant arrays and modified uniformly redundant arrays onto a square lattice, where every opaque element in the aperture is joined to at least one other opaque element. The parameters required to create these high-throughput contiguous apertures are provided, and they have superior imaging quality compared to other known self-supporting apertures.
Article
Biochemical Research Methods
Roberto Rodriguez-Moncayo, Diana F. Cedillo-Alcantar, Pablo E. Guevara-Pantoja, Oriana G. Chavez-Pineda, Jose A. Hernandez-Ortiz, Josue U. Amador-Hernandez, Gustavo Rojas-Velasco, Fausto Sanchez-Munoz, Daniel Manzur-Sandoval, Luis D. Patino-Lopez, Daniel A. May-Arrioja, Rosalinda Posadas-Sanchez, Gilberto Vargas-Alarcon, Jose L. Garcia-Cordero
Summary: The applications of serology tests for SARS-CoV-2 include diagnosing COVID-19, understanding humoral responses, estimating prevalence, and modeling the pandemic. A new high-throughput microfluidic device can assess antibody reactivities to four SARS-CoV-2 antigens from 50 serum samples simultaneously, with high sensitivity and specificity.
Article
Chemistry, Physical
Hao Zhang, Huijie Zhao, Qi Guo, Dong Xu, Wenjie Teng
Summary: Spectral imaging detection using acousto-optical tunable filters (AOTFs) faces a significant challenge of low throughput. To overcome this, a novel polarization multiplexing design is proposed, resulting in a more than two-fold increase in system throughput. The effectiveness of the design in improving system throughput and enhancing the imaging signal-to-noise ratio (SNR) by approximately 8 dB is validated through analysis and experimental results. Additionally, an optimization strategy for arbitrary AOTF devices is proposed, which can achieve similar spectral effects.
Article
Biochemical Research Methods
Chang Hyun Cho, Minkyung Cho, Je-Kyun Park
Summary: The study introduced a multichannel microfluidic immunohistochemistry technology for high-throughput analysis of tissue microarrays, showing great potential for molecular diagnostics.
Article
Environmental Sciences
Wiwik Bauten, Maximilian Noeth, Tetiana Kurkina, Francisca Contreras, Yu Ji, Cloe Desmet, Miguel-Angel Serra, Douglas Gilliland, Ulrich Schwaneberg
Summary: Researchers developed a multiplexed analytical and flow cytometry-based high-throughput method to quantify microplastic particles in aqueous suspensions, providing a sensitive and high-throughput detection capability.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Haoqiang Feng, Shitao Shen, Mingliang Jin, Qilin Zhang, Mengjun Liu, Zihao Wu, Jiamei Chen, Zichuan Yi, Guofu Zhou, Lingling Shui
Summary: This work proposes a method for rapid formation of uniform droplet array called electro-coalescence droplet array (ECDA). The electro-coalescence of droplets is achieved by polarization induced electrostatic and dielectrophoretic forces, and the dielectrowetting effect. Photolithographically fabricated microwells ensure identical volume and physical confinement to achieve uniform droplet array, and microwell isolation protects the paired water droplets from fusion. The optimized ECDA is successfully applied for various microwell geometries and functional materials, and shows potential for high-throughput screening and analysis.
Article
Chemistry, Analytical
Vasyl Yatsyna, Ali H. Abikhodr, Ahmed Ben Faleh, Stephan Warnke, Thomas R. Rizzo
Summary: Coupling vibrational ion spectroscopy with high-resolution ion mobility separation allows for detailed analysis of biomolecules in the gas phase. A novel multiplexed approach using Hadamard transform multiplexing is introduced, which improves the signal-to-noise ratio of infrared spectra compared to conventional methods. This study demonstrates the utility of this approach for cryogenic ion spectroscopy of peptides and glycans in mixtures.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Marie-Ming Aynaud, J. Javier Hernandez, Seda Barutcu, Ulrich Braunschweig, Kin Chan, Joel D. Pearson, Daniel Trcka, Suzanna L. Prosser, Jaeyoun Kim, Miriam Barrios-Rodiles, Mark Jen, Siyuan Song, Jess Shen, Christine Bruce, Bryn Hazlett, Susan Poutanen, Liliana Attisano, Rod Bremner, Benjamin J. Blencowe, Tony Mazzulli, Hong Han, Laurence Pelletier, Jeffrey L. Wrana
Summary: The study introduces a platform called C19-SPAR-Seq, which can analyze tens of thousands of patient samples for SARS-CoV-2 in a single run, with high specificity and sensitivity demonstrated through experimental trials in clinical diagnostics.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Neda Rafat, Lee Brewer, Nabojeet Das, Dhruti J. Trivedi, Balazs K. Kaszala, Aniruddh Sarkar
Summary: Multiplexed biomarker detection is crucial for disease diagnosis and outcome prediction, but the current gold standard method, ELISA, is expensive, limited, and only detects one biomarker at a time. We introduce a low-cost, smartphone-based biosensing platform for high-throughput, multiplexed, and quantitative biomarker detection from small clinical samples. This platform utilizes enzymatic metallization to convert biomarker binding to silver metal spots, which can be easily detected and quantified using a custom smartphone application. We demonstrate its capability for detecting multiple viral antigen-specific antibodies from COVID-19 patients and vaccine-elicited antibody responses from vaccinated individuals.
Review
Biotechnology & Applied Microbiology
Chun-Yi Chang, Chien-Chi Lin
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a common and aggressive form of pancreatic cancer with limited survival rate improvement, mainly due to its resistance to chemotherapy. Hydrogel-based tumor models can help in understanding how matrix properties affect cancer cell growth, migration, and drug resistance.
BIOENGINEERING-BASEL
(2021)
Article
Biology
Sumana Sharma, Toby Whitehead, Mateusz Kotowski, Emily Zhi Qing Ng, Joseph Clarke, Judith Leitner, Yi-Ling Chen, Ana Mafalda Santos, Peter Steinberger, Simon J. Davis
Summary: This study used CRISPR-based genome screening and Jurkat cell-based transcriptional reporter assay to investigate the signaling pathways of T-cell activation and inhibition. The results confirmed the utility of arrayed and pooled screens in studying different mediators. SHP2 was identified as the major PD-1-specific, SH2 family mediator of inhibitory signaling.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biotechnology & Applied Microbiology
Jase Gehring, Jong Hwee Park, Sisi Chen, Matthew Thomson, Lior Pachter
NATURE BIOTECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Jesse Q. Zhang, Kai-Chun Chang, Leqian Liu, Zev J. Gartner, Adam R. Abate
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Biochemical Research Methods
Kenneth H. Hu, John P. Eichorst, Chris S. McGinnis, David M. Patterson, Eric D. Chow, Kelly Kersten, Stephen C. Jameson, Zev J. Gartner, Arjun A. Rao, Matthew F. Krummel
Review
Cell Biology
Vasudha Srivastava, Tyler R. Huycke, Kiet T. Phong, Zev J. Gartner
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Biology
Christopher S. McGinnis, David A. Siegel, Guorui Xie, George Hartoularos, Mars Stone, Chun J. Ye, Zev J. Gartner, Nadia R. Roan, Sulggi A. Lee
Summary: This study demonstrates that mixing PBMCs from unrelated donors under standard scRNA-seq sample preparation conditions does not induce an allogeneic response, and that certain PBMC preparation methods and sample multiplexing technologies can introduce technical artifacts into scRNA-seq data. These findings provide important benchmarks for future cross-sectional immunological scRNA-seq experiments.
Editorial Material
Chemistry, Physical
Zev J. Gartner, Jennifer L. Hu
Summary: This bioprinting approach utilizes organoid-forming stem cells as living ink within hydrogels, guiding tissue-scale self-organization to generate more realistic gastrointestinal and vascular tissue constructs.
Review
Biochemistry & Molecular Biology
Pierre-Francois Lenne, Edwin Munro, Idse Heemskerk, Aryeh Warmflash, Laura Bocanegra-Moreno, Kasumi Kishi, Anna Kicheva, Yuchen Long, Antoine Fruleux, Arezki Boudaoud, Timothy E. Saunders, Paolo Caldarelli, Arthur Michaut, Jerome Gros, Yonit Maroudas-Sacks, Kinneret Keren, Edouard Hannezo, Zev J. Gartner, Benjamin Stormo, Amy Gladfelter, Alan Rodrigues, Amy Shyer, Nicolas Minc, Jean-Leon Maitre, Stefano Di Talia, Bassma Khamaisi, David Sprinzak, Sham Tlili
Summary: The interactions between mechanics and biochemistry in cell and tissue organization have recently attracted renewed interest from various scientific disciplines, with advances in optical physics, microscopy, and computational image analysis enhancing our ability to observe spatiotemporal patterns. In addition, rapid progress in genetic, biophysical, and optogenetic manipulation tools, as well as theory and computing, have allowed for the construction of predictive models describing the emergence of cell and tissue dynamics from the coupling of biochemistry and mechanics.
Article
Developmental Biology
Lain Martyn, Zev J. Gartner
Summary: Embryonic tissue boundaries are crucial for cementing newly patterned structures during development and serving as organizing centers for subsequent rounds of morphogenesis. Understanding the complex functions of these boundaries is essential for creating better self-organizing tissues in basic science, drug development, and regenerative medicine.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Rogelio A. Hernandez-Lopez, Wei Yu, Katelyn A. Cabral, Olivia A. Creasey, Maria Del Pilar Lopez Pazmino, Yurie Tonai, Arsenia De Guzman, Anna Makela, Kalle Saksela, Zev J. Gartner, Wendell A. Lim
Summary: The study engineered a two-step positive-feedback circuit that enables human cytotoxic T cells to discriminate targets based on a sigmoidal antigen-density threshold. This circuit involves a low-affinity synthetic Notch receptor controlling the expression of a high-affinity CAR, leading to sharp discrimination between cancer cells and normal cells based on antigen density.
Article
Multidisciplinary Sciences
Jesse Q. Zhang, Christian A. Siltanen, Ata Dolatmoradi, Chen Sun, Kai-Chun Chang, Russell H. Cole, Zev J. Gartner, Adam R. Abate
Summary: An automated approach using a commercial array spotter was described for generating droplet libraries quickly and efficiently, making combinatorial applications more feasible in droplet microfluidics. The method can produce high diversity droplet libraries without the need for microfluidic expertise.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Katelyn A. Cabral, David M. Patterson, Olivia J. Scheideler, Russell Cole, Adam R. Abate, David Schaffer, Lydia L. Sohn, Zev J. Gartner
Summary: The DPAC micropatterning technique uses DNA hybridization to assemble cells on substrates, adhering to complementary DNA sequences, allowing for precise patterning of different cell types.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Abigail A. Kindberg, Vasudha Srivastava, Jonathon M. Muncie, Valerie M. Weaver, Zev J. Gartner, Jeffrey O. Bush
Summary: The study reveals that EPH/EPHRIN signaling decreases the stability of heterotypic cell:cell contacts through increased cortical actomyosin contractility, driving cellular self-organization. The cell segregation driven by EPH/EPHRIN depends on actomyosin contractility but occurs independently of directed cell migration and without changes in cell adhesion.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Berna Sozen, Victoria Jorgensen, Bailey A. T. Weatherbee, Sisi Chen, Meng Zhu, Magdalena Zernicka-Goetz
Summary: In this study, expanded pluripotent stem cells were used to model early human embryo development, generating self-organizing cystic structures displaying early embryonic characteristics. While there are similarities in cell lineages, morphology, and signaling pathways, significant divergences from natural blastocysts persist. This method provides insights into the design of stem cell models for embryogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Katelyn A. Cabral, Vasudha Srivastava, Austin J. Graham, Maxwell C. Coyle, Connor Stashko, Valerie Weaver, Zev J. Gartner
Summary: The construction of three-dimensional microvascular networks remains challenging. In this study, we used bioprinting to pattern endothelial cells and investigated the microenvironmental cues necessary for their self-organization into microvessels. We found that the inclusion of fibrillar matrices and the absence of certain proteins in the culture medium promoted the formation of stable and perfusable microvascular networks in as little as 3 days.
TISSUE ENGINEERING PART A
(2023)
Article
Multidisciplinary Sciences
Sisi Chen, Paul Rivaud, Jong H. Park, Tiffany Tsou, Emeric Charles, John R. Haliburton, Flavia Pichiorri, Matt Thomson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
