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Biochemistry & Molecular Biology
Yu-Chao Mei, Jiangpeng Feng, Fei He, Yu-Min Li, Yafei Liu, Feng Li, Yu Chen, Hai-Ning Du
Summary: The study demonstrates the crucial role of Set2-mediated H3K36 methylation in repressing antisense transcription, which may be involved in DNA replication and damage repair.
Article
Multidisciplinary Sciences
Haipeng Guan, Pei Wang, Pei Zhang, Chun Ruan, Yutian Ou, Bo Peng, Xiangdong Zheng, Jianlin Lei, Bing Li, Chuangye Yan, Haitao Li
Summary: The Rpd3S complex recognizes and deacetylates histones H3 and H4 at transcribed regions, guided by H3K36me3 modifications. Cryo-electron microscopy structures of Rpd3S in free and H3K36me3 nucleosome-bound states reveal a unique architecture. Multivalent recognition of H3K36me3 marks, nucleosomal DNA, and linker DNAs positions the catalytic center for deacetylation. Combinatorial readout of unmethylated H3K4 and H3K36me3 directs specific histone H3 deacetylation.
Article
Biochemistry & Molecular Biology
Bo Bae Lee, Hyeonju Woo, Min Kyung Lee, SeoJung Youn, Sumin Lee, Jae-Seok Roe, Soo Young Lee, TaeSoo Kim
Summary: Chromatin-based regulation of internal cryptic promoters is mediated by core promoter strength as well as transcription elongation factors.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell Biology
Yu -Min Li, Yu -Chao Mei, Ao-Hui Liu, Ru-Xin Wang, Runfa Chen, Hai-Ning Du
Summary: Loss of H3K36me3 during aging is caused by the degradation of Set2 through the actions of Ubc3 and Bre1. Lack of Bre1 or removal of ubiquitination stabilizes Set2, sustains H3K36me3 levels, and extends lifespan.
Article
Microbiology
Yonathan Lukito, Kate Lee, Nazanin Noorifar, Kimberly A. Green, David J. Winter, Arvina Ram, Tracy K. Hale, Tetsuya Chujo, Murray P. Cox, Linda J. Johnson, Barry Scott
Summary: Recent studies have identified key genes that control the symbiotic interaction between Epichloe festucae and Lolium perenne, as well as specific E. festucae genes that control host infection. Deletion of these genes leads to severe defects in colony growth and hyphal development, resulting in complete loss of ability to infect the host plant. Transcriptome analysis identified fungal candidate genes differentially regulated in mutants compared to wild type, highlighting the importance of these genes in host infection.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rui Shao, Jinlong Suo, Zhong Zhang, Mingxiang Kong, Yiyang Ma, Yang Wen, Mengxue Liu, Lenan Zhuang, Kai Ge, Qing Bi, Changqing Zhang, Weiguo Zou
Summary: This study found that H3K36 methylation is decreased in senescent chondrocytes and osteoarthritic cartilage. Nsd1 plays an important role in articular cartilage homeostasis and OA, with OSR2 possibly being a key factor regulated by Nsd1. Targeting H3K36 methylation and OSR2 could be a novel strategy for OA treatment.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Cell Biology
Dong-Hyuk Heo, Krzysztof Kus, Pawel Grzechnik, Sue Mei Tan-Wong, Adrien Birot, Tea Kecman, Soren Nielsen, Nikolay Zenkin, Lidia Vasiljeva
Summary: Phosphorylation of the RNA polymerase II C-terminal domain Y1S2P3T4S5P6S7 consensus sequence is crucial for coordinating key events in transcription, and its dysregulation can lead to defects in transcription and RNA processing. The histone deacetylase activity of the fission yeast Hos2/Set3 complex has been found to play a significant role in suppressing cryptic initiation of antisense transcription, particularly when RNA polymerase II phosphorylation is dysregulated. Loss of Hos2 or Set3, combined with the absence of Ssu72 phosphatase, results in a synergistic increase in antisense transcription globally and higher sensitivity to genotoxic agents.
Article
Cell Biology
Tatsuya Ohhata, Kazuki Yamazawa, Asuka Miura-Kamio, Saori Takahashi, Satoshi Sakai, Yuka Tamura, Chiharu Uchida, Kyoko Kitagawa, Hiroyuki Niida, Ichiro Hiratani, Hisato Kobayashi, Hiroshi Kimura, Anton Wutz, Masatoshi Kitagawa
Summary: This study highlights the hierarchy of multiple chromatin events upon stepwise gene silencing establishment.
Article
Multidisciplinary Sciences
Yanjun Zhang, Yuan Fang, Yin Tang, Shixun Han, Junqi Jia, Xinyi Wan, Jiaqi Chen, Ying Yuan, Bin Zhao, Dong Fang
Summary: SETD2 methylates histone H3K36me3 in gene bodies in mammalian cells. In this study, the authors demonstrate that H3K36me3 is also enriched at promoter regions and is catalyzed by SMYD5, recruited by RNA polymerase II. They further show that SMYD5 is elevated in liver cancer, with a correlation to changes in gene expression. These findings provide insights into the localization and function of H3K36me3.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Devadathan Valiyamangalath Sethumadhavan, Gayathri Govindaraju, C. A. Jabeena, Arumugam Rajavelu
Summary: Antigenic variation is an essential mechanism for the malaria parasite to establish chronic infection in humans. The study has identified a novel mechanism of H3K36me3 mark propagation on var genes by allosteric regulation of PfSET2 protein by H3K36me2 mark.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Multidisciplinary Sciences
Kartik N. Rajagopalan, Xiao Chen, Daniel N. Weinberg, Haifen Chen, Jacek Majewski, C. David Allis, Chao Lu
Summary: Hotspot histone H3 mutations, particularly H3K36M, play a significant role in oncogenesis in various cancers. Depletion of H3K36me2, but not H3K36me3, largely recapitulates the effects of H3K36M on redistribution of H3K27me3 and gene expression, indicating a functional divergence between these two methylation states in H3K36M-driven oncogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jeong Hyun Ahn, Eric S. Davis, Timothy A. Daugird, Shuai Zhao, Ivana Yoseli Quiroga, Hidetaka Uryu, Jie Li, Aaron J. Storey, Yi-Hsuan Tsai, Daniel P. Keeley, Samuel G. Mackintosh, Ricky D. Edmondson, Stephanie D. Byrum, Ling Cai, Alan J. Tackett, Deyou Zheng, Wesley R. Legant, Douglas H. Phanstiel, Gang Greg Wang
Summary: The development of cancer is closely associated with genetic abnormalities targeting proteins with intrinsically disordered regions (IDRs), which play a crucial role in leukaemic transformation. The presence of IDRs within chimera transcription factors promotes liquid-liquid phase separation (LLPS), enhancing their genomic targeting and inducing aberrant three-dimensional chromatin structure during tumourous transformation.
Article
Biochemistry & Molecular Biology
Yuan Fang, Yin Tang, Yanjun Zhang, Yixin Pan, Junqi Jia, Zhongxing Sun, Weiwu Zeng, Jiaqi Chen, Ying Yuan, Dong Fang
Summary: Epigenetics, especially histone marks, plays a crucial role in regulating gene expression beyond DNA sequences. NSD1 depletion leads to both up- and down-regulation of gene expression, with an increase in H3K27ac correlating with the decrease of H3K36me2. NSD1 acts as a 'safeguard' by depositing H3K36me2 and recruiting HDAC1 at active enhancers to prevent further activation of active enhancer-associated genes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Oncology
Juyeong Hong, Ji Hoon Lee, Zhao Zhang, Yanming Wu, Mei Yang, Yiji Liao, Richard de la Rosa, Jessica Scheirer, Douglas Pechacek, Nu Zhang, Zhenming Xu, Tyler Curiel, Xi Tan, Tim H. -M. Huang, Kexin Xu
Summary: Inhibition of cancer-intrinsic EZH2 promotes antitumor immunity in breast cancer, requiring specific activation of type I IFN signaling. High levels of EZH2 and low levels of STAT2 in breast cancer patients are associated with the worst antitumor immune responses.
Review
Biochemistry & Molecular Biology
Jungmin Choi, Zae Young Ryoo, Dong-Hyung Cho, Hyun-Shik Lee, Hong-Yeoul Ryu
Summary: The interaction between DNA-winding proteins plays a crucial role in ensuring accurate gene expression and preventing aberrant gene expression linked to diseases like cancer and neurodegenerative disorders. Crosstalk between different histone proteins regulates their functions by chemically modifying each other's structure. Recent studies have revealed the importance of trans-tail regulation in maintaining transcriptional fidelity and its role in coordinating various histone modifications during cotranscriptional processes.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Bony De Kumar, Mark E. Parrish, Brian D. Slaughter, Jay R. Unruh, Madelaine Gogol, Christopher Seidel, Ariel Paulson, Hua Li, Karin Gaudenz, Allison Peak, William McDowell, Brian Fleharty, Youngwook Ahn, Chengqi Lin, Edwin Smith, Ali Shilatifard, Robb Krumlauf
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Biology
Jason A. Morrison, Rebecca McLennan, Lauren A. Wolfe, Madeleine M. Gogol, Samuel Meier, Mary C. McKinney, Jessica M. Teddy, Laura Holmes, Craig L. Semerad, Andrew C. Box, Hua Li, Kathryn E. Hall, Anoja G. Perera, Paul M. Kulesa
Article
Biology
Seunghee Oh, Tamaki Suganuma, Madelaine M. Gogol, Jerry L. Workman
Article
Cell Biology
Chengqi Lin, Alexander S. Garrett, Bony De Kumar, Edwin R. Smith, Madelaine Gogol, Christopher Seidel, Robb Krumlauf, Ali Shilatifard
GENES & DEVELOPMENT
(2011)
Article
Cell Biology
Arnob Dutta, Madelaine Gogo, Jeong-Hoon Kim, Michaela Smolle, Swaminathan Venkatesh, Joshua Gilmore, Laurence Florens, Michael P. Washburn, Jerry L. Workman
GENES & DEVELOPMENT
(2014)
Article
Cell Biology
Jennifer M. Gardner, Christine J. Smoyer, Elizabeth S. Stensrud, Richard Alexander, Madelaine Gogol, Winfried Wiegraebe, Sue L. Jaspersen
JOURNAL OF CELL BIOLOGY
(2011)
Article
Multidisciplinary Sciences
Swaminathan Venkatesh, Michaela Smolle, Hua Li, Madelaine M. Gogol, Malika Saint, Shambhu Kumar, Krishnamurthy Natarajan, Jerry L. Workman
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Genetics & Heredity
Qiye He, Anais F. Bardet, Brianne Patton, Jennifer Purvis, Jeff Johnston, Ariel Paulson, Madelaine Gogol, Alexander Stark, Julia Zeitlinger
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Biochemistry & Molecular Biology
Michaela Smolle, Swaminathan Venkatesh, Madelaine M. Gogol, Hua Li, Ying Zhang, Laurence Florens, Michael P. Washburn, Jerry L. Workman
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2012)
Article
Genetics & Heredity
Danny E. Miller, Satomi Takeo, Kavyasree Nandanan, Ariel Paulson, Madelaine M. Gogol, Aaron C. Noll, Anoja G. Perera, Kendra N. Walton, William D. Gilliland, Hua Li, Karen K. Staehling, Justin P. Blumenstiel, R. Scott Hawley
G3-GENES GENOMES GENETICS
(2012)
Article
Genetics & Heredity
Jennifer M. Friederichs, Jennifer M. Gardner, Christine J. Smoyer, Christine R. Whetstine, Madelaine Gogol, Brian D. Slaughter, Sue L. Jaspersen
G3-GENES GENOMES GENETICS
(2012)
Article
Genetics & Heredity
Ben J. Vincent, Gavin R. Rice, Gabriella M. Wong, William J. Glassford, Kayla Downs, Jessica L. Shastay, Kenechukwu Charles-Obi, Malini Natarajan, Madelaine Gogol, Julia Zeitlinger, Mark Rebeiz
G3-GENES GENOMES GENETICS
(2019)
Article
Multidisciplinary Sciences
Shihao Zhang, Xilan Yu, Yuan Zhang, Xiangyan Xue, Qi Yu, Zitong Zha, Madelaine Gogol, Jerry L. Workman, Shanshan Li
Summary: Telomeres, organized into heterochromatin, require maintenance of silent heterochromatin for chromosome stability. The study shows that Pyruvate kinase Pyk1 phosphorylates histone H3T11 and regulates gene expression through the SESAME complex. SESAME phosphorylates H3T11 at telomeres to maintain SIR complex occupancy and prevent autophagy-mediated Sir2 degradation, ultimately enhancing telomere silencing and protecting against compromised telomere silencing during aging.
NATURE COMMUNICATIONS
(2021)
Meeting Abstract
Genetics & Heredity
Adrian McNairn, Scarlett Gard, William Light, Tania Bose, Bo Xiong, Chun Ruan, Karin Zueckert-Gaudenz, Madelaine Gogol, Brian Fleharty, Chris Seidel, Jason Brickner, Jennifer L. Gerton
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2010)