期刊
CHEMICAL SCIENCE
卷 7, 期 2, 页码 1245-1256出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5sc03519h
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资金
- Office of the Vice President for Research & Economic Development
- Jack N. Averitt College of Graduate Studies
- St. Bonaventure University
- Keenan-Martine grant
- Institute for Basic Science [IBS-R019-D1]
- Ministry of Education
- National Research Foundation of Korea
- National Institutes of Health [CA 68682]
- NATIONAL CANCER INSTITUTE [R01CA068682] Funding Source: NIH RePORTER
Ferrocene containing N-heterocyclic carbene (NHC) ligated gold(I) complexes of the type [Au(NHC)(2)](+) were prepared and found to be capable of regulating the formation of reactive oxygen species (ROS) via multiple mechanisms. Single crystal X-ray analysis of bis(1-(ferrocenylmethyl)-3-mesitylimidazol-2-ylidene)-gold(I) chloride (5) and bis(1,3-di(ferrocenylmethyl)imidazol-2-ylidene)-gold(I) chloride (6) revealed a quasi-linear geometry around the gold(I) centers (i.e., the C-Au-C bond angle were measured to be similar to 177 degrees and all the Au-C-carbene bonds distances were in the range of 2.00 (7)-2.03 (1) angstrom). A series of cell studies indicated that cell proliferation inhibition and ROS generation were directly proportional to the amount of ferrocene contained within the [Au(NHC)(2)](+) complexes (IC50 of 6 < 5 < bis(1-benzyl-3-mesitylimidazol-2-ylidene)-gold(I) chloride (4)). Complexes 4-6 were also confirmed to inhibit thioredoxin reductase as inferred from lipoate reduction assays and increased chelatable intracellular zinc concentrations. RNA microarray gene expression assays revealed that 6 induces endoplasmic reticulum stress response pathways as a result of ROS increase.
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