Article
Engineering, Environmental
Florent Rossi, Louis Carles, Florence Donnadieu, Isabelle Batisson, Joan Artigas
Summary: The study investigates the individual degrading behavior of bacterial strains isolated from glyphosate-degrading stream biofilms, revealing different and complementary glyphosate degradation pathways for bacteria co-existing in stream biofilms. Different bacterial strains showed variations in the degradation speed, pathway utilization, and accumulation of degradation products for glyphosate and its metabolite AMPA.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Food Science & Technology
Giulia Rampazzo, Teresa Gazzotti, Elisa Zironi, Giampiero Pagliuca
Summary: Hive products can be affected by herbicides like glyphosate and glufosinate, which may harm bees and humans. This review collects and compares data from various studies to provide an overview of the presence of these herbicides and their metabolites in hive products, as well as their potential impacts on health and recent regulatory developments. The data shows that these substances can be found in hive products, but usually at lower concentrations than the maximum residue limits. However, the use of different analytical methods and incomplete search for regulated compounds make comparisons challenging. Further studies and harmonization of analytical methodologies and data management procedures are necessary.
Article
Chemistry, Multidisciplinary
Diego F. Hernadndez-Barreto, Heriberto Hernandez-Cocoletzi, Juan Carlos Moreno-Pirajan
Summary: This study verified the adsorption effect of biogenic hydroxyapatite on glyphosate through experiments and theoretical calculations, indicating its potential application prospects in aqueous solutions.
Article
Multidisciplinary Sciences
Yoshihiro Furukawa, Yoshinari Iwasa, Yoshito Chikaraishi
Summary: Research shows that solvent-soluble organic matter in meteorites from the early solar system may have originated from amino acid synthesis associated with formose-type reactions, not just limited to cold regions. These results indicate that different types of chemical reactions in the early solar system may have led to the formation of C-13-enriched organic matter.
Article
Microbiology
Veronica Spinelli, Andrea Ceci, Chiara Dal Bosco, Alessandra Gentili, Anna Maria Persiani
Summary: This study focused on examining the tolerance and degradation ability of various fungi to glyphosate, with Purpureocillium lilacinum found to have a considerable 80% degradation capability without negative effects on growth. It was also noted that pure glyphosate is more toxic than Roundup for P. lilacinum, and the decreased pH in the culture medium only partially alleviates the toxicity of pure glyphosate.
Review
Endocrinology & Metabolism
Maria Florencia Rossetti, Guillermina Canesini, Virginia Lorenz, Maria Mercedes Milesi, Jorgelina Varayoud, Jorge Guillermo Ramos
Summary: Glyphosate and its metabolites can induce epigenetic changes in humans and rodents, affecting global DNA methylation, gene-specific methylation, histone modification, and differential expression of non-coding RNAs. These changes may have heritable implications and could lead to disease long after exposure has ceased.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Plant Sciences
Alexi C. Besser, Emma A. Elliott Smith, Seth D. Newsome
Summary: Analyzing isotopes of amino acids can offer more accurate estimates of energy flow and consumer trophic levels in food webs. However, the specific analysis of amino acids in terrestrial and freshwater producers still requires further study and exploration.
JOURNAL OF ECOLOGY
(2022)
Article
Chemistry, Analytical
Zahra Ramezani, Mehdi Safdarian, Ata A. Ghadiri
Summary: In this study, a novel sarcosine metal-coded hydrogel magnetic molecularly imprinted polymer was successfully fabricated and applied for the extraction and quantification of sarcosine in urine samples. The method employed on-column derivatization capillary electrophoresis to separate and detect multiple amino acids, achieving satisfactory detection results.
Article
Chemistry, Applied
Ana Paula Ferreira de Souza, Nadia Regina Rodrigues, Felix Guillermo Reyes Reyes
Summary: The study found residues of glyphosate and aminomethylphosphonic acid (AMPA) in Brazilian honey, with some samples showing glyphosate levels exceeding the EU maximum residue limit. This indicates a potential impact on bee populations in regions with high losses of bee colonies and frequent use of glyphosate in agriculture.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2021)
Review
Biochemistry & Molecular Biology
Ahmad Abuaisheh, Orwa Aboud
Summary: Gliomas are common primary brain tumors that arise from neuroglial cells or progenitor cells. They can be classified as low-grade or high-grade based on WHO classification. Despite extensive research, high-grade gliomas still have a poor prognosis. Biogenic amines, known for their toxic and carcinogenic properties, have not been fully studied in relation to gliomas. This review aims to investigate the roles of biogenic amines in glioma development, diagnostics, and potential treatments.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Review
Biochemistry & Molecular Biology
Suzeeta Bhandari, Kirpal S. Bisht, David J. Merkler
Summary: Fatty acid amides are lipids composed of a fatty acid and a biogenic amine. This review focuses on a lesser-known sub-class of fatty acid amides called N-acyl aromatic amino acids (NA-ArAAs). The review highlights the identified NA-ArAAs from biological sources and presents information on their biosynthesis, degradation, enzymatic modification, and transport, as well as their cellular functions. The aim of this review is to stimulate future research on this underappreciated sub-class of the fatty acid amide family.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Food Science & Technology
D. Sylvain Dabade, Liesbeth Jacxsens, Lisa Miclotte, Emmanuel Abatih, Frank Devlieghere, Bruno De Meulenaer
Summary: This study quantitatively determined multiple biogenic amines in various food products sold in the Belgian market, and found significant positive correlations between BA concentrations and corresponding precursor FAAs, with stronger correlations in meat-based products. The study provides a basis for assessing the potential public health impact of BAs, which is currently limited to histamine in scombroid fish species and their derived products.
Article
Chemistry, Multidisciplinary
Wojciech Wojcik, Krzysztof Damaziak, Monika Lukasiewicz-Mierzejewska, Olga Swider, Jan Niemiec, Michal Wojcicki, Marek Roszko, Dariusz Gozdowski, Julia Riedel, Agata Marzec
Summary: This study aimed to determine the formation of biogenic amines (BAs) and their precursor amino acids in chicken breast and leg muscles stored under chilling conditions. The results showed a significant increase in BAs in both muscle types during storage, and distinct levels of BAs were detected in the muscles.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Damian Van Raad, Thomas Huber, Gottfried Otting
Summary: Cell-free protein synthesis using eCells enables the production of amino acids from low-cost C-13-labelled precursors. We demonstrate that the metabolic pathway converting pyruvate, glucose, and erythrose into aromatic amino acids remains intact in eCells. Selective C-13 labelling of tyrosine and phenylalanine residues is achieved by adjusting the composition of the reaction buffers.
JOURNAL OF BIOMOLECULAR NMR
(2023)
Article
Food Science & Technology
Gean Charles Monteiro, Igor Otavio Minatel, Adilson Pimentel Junior, Hector Alonzo Gomez-Gomez, Joao Pedro Correa de Camargo, Marla Silvia Diamante, Leticia Silva Pereira Basilio, Marco Antonio Tecchio, Giuseppina Pace Pereira Lima
Summary: This study evaluated the phenolic compounds, antioxidant capacity, biogenic amines, and amino acids in flours obtained from grape pomace, finding valuable potential for new product development in flours from different cultivars or blends, with increased levels of phenolic compounds and antioxidant capacity in blends containing 'Niagara Rosada'. The flour production provides a better destination for industrial waste, as these flours can be used as additives or supplements in various industries to improve antioxidant and bioactive content in their products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Chemical
Hang He, Shizong Wang, Jianlong Wang
Summary: This study investigated the degradation of 3-methylindole using ionizing radiation technology, showing high removal efficiency under specific conditions. Different factors were found to influence the degradation of 3-methylindole, and the toxicity of its intermediate products was lower.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Environmental
Shizong Wang, Jianlong Wang
Summary: Cobalt sulfides as persulfate activator have been studied for their catalytic activity in persulfate-based advanced oxidation process. This study successfully prepared Co9S8 anchored in N, S, O heteroatoms co-doped carbon composite, which showed high catalytic activity in activating peroxymonosulfate for sulfamethoxazole degradation. The study also investigated the effect of various factors on the degradation process and identified the active sites and intermediate products involved.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Shizong Wang, Jun Hu, Jianlong Wang
Summary: In this study, cobalt sulfides (Co9S8) coated on the nitrogen and sulfur co-doped graphene (Co9S8@S-N-RG) were prepared and used for the degradation of antibiotic sulfamethoxazole (SMX). The Co9S8@S-N-RG showed superior catalytic activity, degrading SMX with a mineralization efficiency of 38.7% within 20 minutes. The activation of peroxymonosulfate (PMS) by Co9S8@S-N-RG was attributed to the production of sulfate radicals, with the participation of Co2+ and sulfur species. Carbon defects, CO, pyridinic N, and pyrrolic N also played a role in PMS activation. This study provides insights into the PMS activation mechanism and offers a stable PMS activator.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Ziquan Wang, Fang Wang, Leilei Xiang, Yongrong Bian, Zhiliang Zhao, Zhengyuan Gao, Jingxing Cheng, Andreas Schaeffer, Xin Jiang, Dionysios D. Dionysiou
Summary: This study investigates the application of Fe(VI) for the oxidation of immobilized pyrene on minerals. Different minerals have different pH optima for pyrene degradation, and hydroxyl radical plays a role in the degradation process.
Article
Environmental Sciences
Siqi Wu, Zhongli Chen, Min Zhou, Ying Shao, Chao Jin, Jinjing Tang, Fang Fang, Jinsong Guo, Felix Stibany, Andreas Schaeffer
Summary: The application of plastic mulch films in agricultural production brings convenience but also results in plastic waste that can degrade into microplastics (MPs). The fate of plastic waste and the potential risks associated with the release of plasticizers under freeze-thaw conditions in agricultural ecosystems are not well understood. This study in China reveals that freeze-thaw alternating accelerates the formation of MPs and the release of plasticizers, which can pose a potential risk to exposed organisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Engineering, Environmental
Felix Schmidt, Luca Ledermann, Andreas Schaeffer, Henry J. Snaith, Markus Lenz
Summary: The availability of leached lead from perovskite solar cells is naturally limited in soils and its adverse effects on soil biota are possibly negligible, suggesting that established risk assessment considerations for lead remain valid.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Leilei Xiang, Jean Damascene Harindintwali, Fang Wang, Yongrong Bian, Zhiliang Zhao, Ziquan Wang, Yu Wang, Zhi Mei, Xin Jiang, Andreas Schaeffer, Baoshan Xing
Summary: Pyrolysis of agricultural waste into biochar is an effective solid waste management strategy, and the properties of biochars derived from different agricultural feedstocks have varying effects on soil remediation of PBDE-contaminated soil. Manure-derived biochar (MBC) is suitable for improving soil quality and promoting microbial growth, while straw-derived biochar (SBC) reduces the bioavailability and ecological risk of PBDE and has a strong adsorption capacity for BDE-47. MBC promotes the debromination of BDE-47 by PBDE-degrading microorganisms, while SBC inhibits it due to its adsorption capacity. Both biochars favor Nitrospirae bacteria and promote nitrogen cycling.
Article
Engineering, Environmental
Sohaib Aslam, Yuying Jing, Karolina M. Nowak
Summary: Glyphosate can be biodegraded via the AMPA and the sarcosine/glycine pathways, producing three intermediate products - AMPA, sarcosine or glycine. The nature of non-extractable residues (NERs; harmless biogenic [NERsbiogenic] versus hazardous xenobiotic [NERsxenobiotic]) of the three intermediate compounds in glyphosate biodegradation in soils has not been investigated yet, which is crucial for assessing environmental risks.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Felix Schmidt, Meret Amrein, Sebastian Hedwig, Manuel Kober-Czerny, Adriana Paracchino, Ville Holappa, Riikka Suhonen, Andreas Schaeffer, Edwin C. Constable, Henry J. Snaith, Markus Lenz
Summary: Perovskite solar cells, which rely on the use of lead, face potential environmental concerns. A recycling process using hot water was demonstrated to effectively extract lead from synthetic precursor mixes, plastic-based, and glass-based perovskites with high efficiency. The extracted lead can be precipitated in high purity, allowing for its recovery. This straightforward method mitigates the risk of lead leaching at the end-of-life of perovskite solar cells.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Soil Science
Tiantian Zheng, Anja Miltner, Chao Liang, Karolina M. Nowak, Matthias Kastner
Summary: Microbial biomass residues play an important role in biogeochemical cycling, but the mechanism by which they are sequestered in soil organic matter remains elusive. This study revealed that bacterial biomass is predominantly utilized by fungi and stabilized as fungal necromass, contributing to soil organic carbon sequestration. The study also identified three phases in the metabolism of microbial biomass residues, with the final phase focused on C preservation rather than energy production.
SOIL BIOLOGY & BIOCHEMISTRY
(2023)
Article
Soil Science
Martin Siedt, Eva-Maria Teggers, Volker Linnemann, Andreas Schaeffer, Joost T. van Dongen
Summary: The study aims to investigate the relationship between the substrate quality of different organic materials derived from plant residues and various soil properties and processes. The results show that the carbon availability, C/N ratio, and metal contents of wheat straw, green compost, and biochar varied significantly. Straw amendment increased microbial activity and led to nitrate depletion in well-aerated sandy loam soil. Intense irrigation of straw-amended soil resulted in strong hypoxia and increased leaching of metals. However, the application of mineral fertilizers mitigated the reduction of redox potential and the leaching of metals. The study highlights the substantial effects of organic soil amendments on nutrient mobility and metal toxicity through microbial respiration and hypoxia.
Article
Engineering, Environmental
Lena Benner, Luisa Coder, Alena Reiter, Martina Ross-Nickoll, Andreas Schaeffer
Summary: This study investigated the pesticide load of foraging bumblebees in agricultural and urban areas. Residues of 15 pesticides were found in 184 out of 220 field samples. The study highlighted the significant differences in pesticide load between land-uses in spring.
JOURNAL OF HAZARDOUS MATERIALS ADVANCES
(2023)
Article
Engineering, Environmental
Andreas Schaeffer, Ksenia J. Groh, Gabriel Sigmund, David Azoulay, Thomas Backhaus, Michael G. Bertram, Bethanie Carney Almroth, Ian T. Cousins, Alex T. Ford, Joan O. Grimalt, Yago Guida, Maria C. Hansson, Yunsun Jeong, Rainer Lohmann, David Michaels, Leonie Mueller, Jane Muncke, Gunilla Oberg, Marcos A. Orellana, Edmond Sanganyado, Ralf Bernhard Schaefer, Ishmail Sheriff, Ryan C. Sullivan, Noriyuki Suzuki, Laura N. Vandenberg, Marta Venier, Penny Vlahos, Martin Wagner, Fang Wang, Mengjiao Wang, Anna Soehl, Marlene Agerstrand, Miriam L. Diamond, Martin Scheringer
Summary: Pollution caused by chemicals and waste, along with climate change and biodiversity loss, poses a triple planetary crisis. In response, countries have established an intergovernmental science-policy panel to address chemicals, waste, and pollution prevention. Protecting this panel from conflicts of interest is crucial for its success.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Dieter Hennecke, Mike Kruse, Joana Braeutigam, Boris Meisterjahn, Judith Klein, Daniela Classen, Stefan Trapp, Matthias Kaestner, Andreas Libonati Brock, Andreas Schaeffer
Summary: This article discusses non-extractable residues (NER) produced by chemicals during degradation testing and their inclusion in environmental persistence assessment. Different types of NER can be experimentally distinguished, and residues of the parent substance in type I NER can be identified.
ENVIRONMENTAL SCIENCE-ADVANCES
(2023)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.