Article
Reproductive Biology
Yufei Huang, Xiaoya Chu, Yafei Zhang, Sheng Yang, Yonghong Shi, Jingxian Wu, Qiusheng Chen
Summary: This study investigated the morphological changes in the testis of adult Shaoxing ducks infected with DTMUV. The results showed that DTMUV infection caused significant atrophy in the testis, cytoplasmic vacuolation and exfoliation in the seminiferous epithelium, decrease in the diameter of seminiferous tubule, and interstitial inflammation. Immunohistochemistry confirmed that spermatogenic cells, especially spermatocytes, were the target cells of DTMUV infection in the testis. Single and clustered virus particles were observed in the spermatogenic cells of infected ducks.
Article
Microbiology
Sheng Yang, Yonghong Shi, Jingxian Wu, Qiusheng Chen
Summary: This study systematically investigates the ultrastructural pathology of the central nervous system (CNS) of ducklings and adult ducks infected with Duck Tembusu virus (DTMUV) using transmission electron microscopy technology. The results show that DTMUV causes extensive brain lesions in ducklings and slight damage in adult ducks. Neurons are the target cells of DTMUV, and virions are mainly observed in their endoplasmic reticulum and Golgi apparatus. Additionally, DTMUV infection leads to swelling in astrocytic foot processes in ducklings and myelin lesions in both ducklings and adult ducks.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Yuhong Pan, Xuedong Wu, Wenjun Cai, Anchun Cheng, Mingshu Wang, Shun Chen, Juan Huang, Qiao Yang, Ying Wu, Di Sun, Sai Mao, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Qun Gao, Xumin Ou, Bin Tian, Zhongqiong Yin, Renyong Jia
Summary: This study utilized high-throughput RNA sequencing to investigate the changes in host gene expression during duck embryo fibroblasts (DEF) infected with Duck Tembusu virus (DTMUV). The results identified a large number of differentially expressed genes related to various biological functions including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. These findings provide valuable insights into the host-DTMUV interaction and have implications for the development of antiviral drugs and vaccines for poultry.
VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Bin Tian, Dongjie Cai, Mingshu Wang, Tianqiong He, Liyao Deng, Liping Wu, Renyong Jia, Dekang Zhu, Mafeng Liu, Shun Chen, Qiao Yang, Ying Wu, Xinxin Zhao, Shaqiu Zhang, Mujeeb Ur Rehman, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Xinjian Wen, Di Sun, Yanling Yu, Ling Zhang, Yunya Liu, Leichang Pan, Xiaoyue Chen, Anchun Cheng
Summary: The study demonstrated that SC75741 exhibits strong antiviral activity against Duck Tembusu virus and Duck plague virus by blocking viral entry and genomic replication, making it a potential antiviral agent for DTMUV and DPV.
Article
Agriculture, Dairy & Animal Science
Juan Huang, Lin Lei, Min Cui, Anchun Cheng, Mingshu Wang, Mafeng Liu, Dekang Zhu, Shun Chen, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Zhongqiong Yin, Renyong Jia
Summary: miR-146b-5p regulates replication of DTMUV by targeting RPS14.
Article
Veterinary Sciences
Yunzhen Huang, Ziqiang Hu, Jiawen Dong, Linlin Li, Junqin Zhang, Ruihuan Kuang, Shimin Gao, Minhua Sun, Ming Liao
Summary: In this study, a chicken-origin TMUV strain was isolated from laying hens with egg drop syndrome in south China. The results showed that this strain exhibited high infectivity and pathogenicity in both chicks and ducklings, suggesting its potential for widespread infection of chickens and ducks.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Tianxu Li, Yanyan Ren, Tingting Zhang, Xinyu Zhai, Xiuyuan Wang, Jinchao Wang, Bin Xing, Runchun Miao, Ning Li, Liangmeng Wei
Summary: This study investigates the regulatory role of duLGP2 in the duck RIG-I-mediated antiviral innate immune signaling system. The results demonstrate that duLGP2 can suppress duRIG-I activation and enhance the production of inflammatory cytokines. These findings contribute to a better understanding of the regulatory networks of the antiviral innate immune system in ducks.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Xuemei Xian, Sheng Yang, Yonghong Shi, Qiusheng Chen
Summary: In this study, it was found that the Duck Tembusu virus (DTMUV) enters cells through the pathway of vesicular endocytosis, and undergoes maturation and replication in the rough endoplasmic reticulum (RER) cisternae. The virus particles are then released from the infected cells after reaching the cell membrane via the Golgi body's endocrine channel. The entry and replication of DTMUV in mammalian and avian cells differ, and can be blocked by a clathrin inhibitor, chlorpromazine (CPZ).
Article
Agriculture, Dairy & Animal Science
Sheng Yang, Jingxian Wu, Yufei Huang, Yafei Zhang, Yonghong Shi, Ping Yang, Qiusheng Chen
Summary: This study constructed a DTMUV-infected duckling model to investigate apoptosis in the duckling brains. It was found that DTMUV infection could induce apoptosis in the brains of ducklings, and the occurrence of apoptosis was accompanied by the virus infection process with certain regularity.
Article
Immunology
Peng Zhou, Lei Ma, Zaixiao Rao, Yaqian Li, Huijun Zheng, Qigai He, Rui Luo
Summary: The Duck Tembusu virus (DTMUV) is a pathogenic flavivirus that impacts egg production and neurological disorders in waterfowl. Proteomics analysis revealed duIFI35 expression promotes DTMUV replication and inhibits IFN-beta production, disrupting the host antiviral response. Specifically, duIFI35 impairs duRIG-I recognition of double-stranded RNA, evading the immune response and providing insights for antiviral drug design.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Wikanda Tunterak, Duangduean Prakairungnamthip, Patchareeporn Ninvilai, Sonthaya Tiawsirisup, Kanisak Oraveerakul, Jiroj Sasipreeyajan, Alongkorn Amonsin, Aunyaratana Thontiravong
Summary: DTMUV, a mosquito-borne flavivirus, has emerged as a causative agent of viral disease in ducks in Thailand, with infections occurring year-round and varying patterns between farm and free-grazing duck systems, peaking in the winter season. Routine surveillance is essential for early detection and control measures.
Article
Infectious Diseases
Nichapat Yurayart, Patchareeporn Ninvilai, Theeraphap Chareonviriyaphap, Theerayuth Kaewamatawong, Aunyaratana Thontiravong, Sonthaya Tiawsirisup
Summary: The study found that Thai DTMUV caused disease in BALB/c mice through intracerebral inoculation, resulting in systemic and neurological symptoms, with pathological changes and virus distribution observed in all tested organs. The viral load was significantly higher in the brain than in other organs, and the virus caused acute death in the mice. Virus shedding was not found in saliva and feces, indicating the potential for Thai DTMUV to transmit disease in mammalian hosts.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Microbiology
Miao Zeng, Wei Zhang, Bowen Jiang, Tong Lu, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Ling Zhang, Anchun Cheng, Andres Merits, Shun Chen
Summary: By studying the role of Flavivirus nonstructural protein 5 (NS5) in TMUV replication and proliferation, it was found that the MTase-RdRp interface and the GTR linker play important roles in the affinity of NS5 protein. Further mutation analysis also provided insights into the replication ability and properties of TMUV.
VETERINARY MICROBIOLOGY
(2022)
Article
Infectious Diseases
Aunyaratana Thontiravong, Teerawut Nedumpun, Patchareeporn Ninvilai, Wikanda Tunterak, Navapon Techakriengkrai, Wijit Banlunara, Sanipa Suradhat
Summary: DTMUV infection significantly impacts the immune components and functions of ducks, leading to the loss of non-T and B lymphocyte/myeloid cell subpopulations and reduced phagocytic activity. However, T and B cell numbers increase during the early phase of infection. There is a negative correlation between CD4(+) and CD8(+) T, B and non-T and B lymphocyte levels and viral loads. Furthermore, DTMUV infection induces an early and robust neutralizing antibody response.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Hantai Tan, Senzhao Zhang, Wangyang Tan, Tao Hu, Yu He, Zhen Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumi Ou, Qun Gao, Di Sun, Anchun Cheng, Shun Chen
Summary: Since 2010, the duck Tembusu virus (DTMUV) has caused a severe outbreak of egg drop syndrome in laying ducks in China. In this study, 6 mAbs that could specifically recognize DTMUV NS1 were identified. Four shortest linear epitopes on NS1 were also identified, along with cross-reaction of some mAbs with other flaviviruses.
Article
Virology
Mohamed S. H. Hassan, Ahmed Ali, Motamed Elsayed Mahmoud, Danah Altakrouni, Shahnas M. Najimudeen, Mohamed Faizal Abdul-Careem
Summary: This study compared the efficacy of different vaccination programs against Canadian DMV/1639 IBV and found that the autogenous inactivated DMV/1639 vaccine was more effective in reducing viral loads and boosting virus neutralization titers. Both vaccination programs provided significant protection against pathological effects.
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.
Article
Virology
Xiao Han, Hankun Xu, Yifan Weng, Rong Chen, Jidong Xu, Tong Cao, Renjie Sun, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: Classical swine fever virus (CSFV) can inhibit the host innate immunity by decreasing the level of high mobility group box 1 (HMGB1), and CSFV Npro can evade host antiviral immune response through upregulating HMGB1 acetylation.
Article
Virology
Sunjian Lyu, Fulei Xiong, Tianpeng Qi, Weifeng Shen, Qi Guo, Mingming Han, Li Liu, Weishao Bu, Julin Yuan, Bao Lou
Summary: This study identified and characterized a novel temperate A. hydrophila phage, P05B, which has strong lytic ability and stability across different temperatures and pH values.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Virology
Rui Wang, Qiang Sun, Jinbo Xiao, Congcong Wang, Xiaoliang Li, Jichen Li, Yang Song, Huanhuan Lu, Ying Liu, Shuangli Zhu, Zhijun Liu, Yong Zhang
Summary: Coxsackievirus A6 (CVA6) has emerged as an important agent causing hand, foot, and mouth disease (HFMD). Mutations in residue 64 of the virus have been found to increase resistance to ribavirin and decrease sensitivity to guanidine hydrochloride. This mutation also leads to increased replication fidelity and reduced pathogenicity. These findings provide a foundation for the development of a safe and effective live attenuated CVA6 vaccine.
Article
Virology
Yao Xiong, Keran Ma, Xiao Zou, Yantao Liang, Kaiyang Zheng, Tiancong Wang, Hong Zhang, Yue Dong, Ziyue Wang, Yundan Liu, Hongbing Shao, Andrew Mcminn, Min Wang
Summary: This study isolated a novel virus, vB_VviC_ZQ26, infecting Vibrio cyclitrophicus from coastal waters near Qingdao, China. It was found that vB_VviC_ZQ26 belongs to a new vibriophage-specific family, Coheviridae, and is mainly found in temperate and tropical epipelagic zones.
Article
Virology
Xing Li, Zhiping Ye, Ewan P. Plant
Summary: By analyzing clinical and cultured samples using the same bioinformatic pipeline, it was found that 5' copyback DVGs are prevalent in human clinical samples but not in cultured samples. Furthermore, there are differences in DVG production and composition between in vivo and in vitro infections.
Article
Virology
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Suvrotoa Mitra, Animesh Gope, Mamta Chawla-Sarkar
Summary: Rotavirus is a major cause of viral gastroenteritis in infants and continues to be a severe issue in underdeveloped nations. Recent research has shown that a long non-coding RNA called SLC7A11-AS1 is upregulated during rotavirus infection and plays a role in facilitating the virus's propagation by regulating intracellular glutathione and lipid peroxidation.
Article
Virology
Eiichi Hondo, Tetsufumi Katta, Ayato Sato, Naoya Kadofusa, Tomoki Ishibashi, Hiroshi Shimoda, Hirokazu Katoh, Atsuo Iida
Summary: Bat-borne emerging viruses, such as the Ebola virus and Nipah virus, pose a significant threat to public health. In this study, we identified micafungin as a potential antiviral drug against Pteropine orthoreovirus (PRV) and found that it inhibits viral release in human cells through activating the endoplasmic reticulum stress response pathway. This research provides valuable insights into the development of effective treatments for future virus pandemics.
Article
Virology
M. Joubert, N. van den Berg, J. Theron, V. Swart
Summary: This study investigated the host gene expression in asymptomatic avocado nursery trees infected with Avocado sunblotch viroid (ASBVd) using RNA sequencing. The results showed that 631 genes were differentially expressed during infection, with 63% upregulated. ASBVd infection significantly affected plant defence responses, phytohormone networks, gene expression pathways, secondary metabolism, cellular transport, as well as protein modification and degradation. This study provides insights into the molecular interactions underlying avsunviroid-host interactions.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.
Article
Virology
Manel Essaidi-Laziosi, Francisco J. Perez-Rodriguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A. Mueller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
Summary: This study found that the Omicron variant has different replication characteristics compared to other variants of concern, being able to efficiently produce infectious virus in nasal cells but not lung cells. In addition, the Delta and Omicron variants showed increased infection efficiency in A549 cells. These findings suggest that the variants of concern have better adaptation to humans but do not have an extended host range.
Article
Virology
Martin Faye, Modeste Name Faye, Babacar Ndiaye, Moussa Moise Diagne, Safietou Sankhe, Ndeye Marieme Top, Amadou Diallo, Cheikh Loucoubar, Ndongo Dia, Amadou Alpha Sall, Ousmane Faye
Summary: Genomic surveillance in Senegal since March 2020 has detected the emergence of Omicron BA.4 and BA.5 sublineages in June 2022. Next-generation sequencing and phylogeny analysis of isolates from a cluster of cases in Northern Senegal in July 2022 revealed that the BA.4 cases originated from a XAS recombinant, marking the first reported sequence of this variant from Senegal. Continuous genomic surveillance of positive SARS-CoV-2 samples is crucial.