Review
Oncology
Calum Gabbutt, Nicholas A. Wright, Ann-Marie Baker, Darryl Shibata, Trevor A. Graham
Summary: The dynamical process of cell division that maintains homeostasis in the human body cannot be observed directly. Instead, it is measured through somatic genetic or epigenetic mutations in tissues. Mathematical analysis of somatic clone sizes provides quantitative information on cell birth, death, and replacement rates. This review explores the use of different somatic mutation types for lineage tracing in human tissues, introduces mathematical concepts for inferring dynamic information from clone size data, and discusses the implications of this approach for understanding homeostasis and cancer development.
JOURNAL OF PATHOLOGY
(2022)
Review
Chemistry, Analytical
Xiaohua Jia, Guodong Shen, Jia Jia, Yan Zhang, Dan Zhang, Wanjun Li, Jianjun Zhang, Xinglu Huang, Jie Tian
Summary: This article introduces the importance of cancer stem cells and the method of lineage tracing, as well as the application of molecular imaging in the dynamic detection of cancer stem cells. The article analyzes the current situation and looks forward to the future development of cancer stem cell detection.
Review
Cell Biology
Anna Konturek-Ciesla, David Bryder
Summary: Appropriate production of mature blood cells is essential for organismal health. This article describes tools and methods for tracing HSC lineage in mice and integrating them with single-cell molecular analysis. By combining new insights from experiments with past knowledge, we gain a better understanding of HSC-derived hematopoiesis in humans.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Michael Spencer Chapman, Anna Maria Ranzoni, Brynelle Myers, Nicholas Williams, Tim H. H. Coorens, Emily Mitchell, Timothy Butler, Kevin J. Dawson, Yvette Hooks, Luiza Moore, Jyoti Nangalia, Philip S. Robinson, Kenichi Yoshida, Elizabeth Hook, Peter J. Campbell, Ana Cvejic
Summary: By reconstructing a phylogenetic tree of blood development in healthy human fetuses, it was found that individual haematopoietic progenitors acquire multiple somatic mutations by 18 weeks after conception. The data also support a hypoblast origin of the extra-embryonic mesoderm and primitive blood in humans.
Article
Immunology
Jue Feng, Joseph N. Pucella, Geunhyo Jang, Marcela Alcantara-Hernandez, Samik Upadhaya, Nicholas M. Adams, Alireza Khodadadi-Jamayran, Colleen M. Lau, Marlon Stoeckius, Stephanie Hao, Peter Smibert, Aristotelis Tsirigos, Juliana Idoyaga, Boris Reizis
Summary: This study investigates the developmental origins of dendritic cells (DCs) and reveals the shared origin of pDCs and cDCs, suggesting a revised scheme of DC development.
Review
Biochemistry & Molecular Biology
Maria Figueres-Onate, Rebeca Sanchez-Gonzalez, Laura Lopez-Mascaraque
Summary: Understanding the strategies used to study cell lineages in the brain and their role in deciphering functional clonal relationships among neural cells; future perspectives will focus on studying cell heterogeneity and the origins of different cell lineages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Engineering, Biomedical
Hyuna Kim, Anna Wirasaputra, Farnaz Mohammadi, Aritra Nath Kundu, Jennifer A. E. Esteves, Laura M. M. Heiser, Aaron S. S. Meyer, Shelly R. R. Peyton
Summary: Breast cancer is a major cause of cancer-related deaths worldwide, primarily due to metastasis and dormancy. Dormancy, a prolonged period between tumor resection and relapse, poses significant challenges in current therapies. To overcome this, a live cell lineage approach is used to track and distinguish dormant cells from proliferative and dying cells in various in vitro microenvironments. The study reveals that the proportion of long-term quiescent cells is regulated by both intrinsic and extrinsic factors, with the highest percentage found in 3D collagen gels. This approach is expected to benefit the study of dormant tumor cells for biologists and bioengineers in the dormancy community.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Physiology
Wenzheng Zhang, Chao Gao, Akaki Tsilosani, Rohan Samarakoon, Robert Plews, Paul J. Higgins
Summary: This article summarizes the recent advances in utilizing the Cre-LoxP system for tracking stem/progenitor cells in kidney development and maturity, as well as the development and characterization of various Cre drivers.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Cell & Tissue Engineering
Chase A. Pagani, Amanda K. Huber, Charles Hwang, Simone Marini, Karthik Padmanabhan, Nicholas Livingston, Johanna Nunez, Yuxiao Sun, Nicole Edwards, Yu-Hao Cheng, Noelle Visser, Pauline Yu, Nicole Patel, Joseph A. Greenstein, Husain Rasheed, Reagan Nelson, Karen Kessel, Kaetlin Vasquez, Amy L. Strong, Geoffrey E. Hespe, Jane Y. Song, Deneen M. Wellik, Benjamin Levi
Summary: The research discovered a specific lineage of cells as ectopic bone progenitors in the zeugopod, providing a new perspective on fate specification and multipotency acquired after injury.
Article
Gastroenterology & Hepatology
Lei Zhou, Ken H. O. Yu, Tin Lok Wong, Zhao Zhang, Chun Ho Chan, Jane H. C. Loong, Noelia Che, Hua Jian Yu, Kel Vin Tan, Man Tong, Elly S. Ngan, Joshua W. K. Ho, Stephanie Ma
Summary: This study utilized mouse models to investigate the heterogeneity and dynamics of Prom1+ cells in HCC, revealing their CSC-like properties and clonal expansion within tumors. Labelled Prom1+ cells showed increased tumorigenicity in 3D culture and allotransplantation, while depletion of these cells impeded tumor growth. Single-cell RNA sequencing analysis highlighted the heterogeneity of Prom1+ HCC cells and their potential role in disease progression.
Review
Cell Biology
Yiteng Dang, Steffen Rulands
Summary: Lineage tracing experiments are crucial for studying stem and progenitor cell fate behavior. However, when cell proliferation is high or clone merging and fragmentation occur frequently, interpretation of clonal fate data becomes ambiguous. This article discusses statistical strategies for detecting and resolving clonal fragmentation and merging, as well as algorithms and statistical methods for restoring the clonal provenance of labeled cells and obtaining indirect information on cell fate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dian Yang, Matthew G. Jones, Santiago Naranjo, William M. Rideout III, Kyung Hoi (Joseph) Min, Raymond Ho, Wei Wu, Joseph M. Replogle, Jennifer L. Page, Jeffrey J. Quinn, Felix Horns, Xiaojie Qiu, Michael Z. Chen, William A. Freed-Pastor, Christopher S. McGinnis, David M. Patterson, Zev J. Gartner, Eric D. Chow, Trever G. Bivona, Michelle M. Chan, Nir Yosef, Tyler Jacks, Jonathan S. Weissman
Summary: Tumor evolution is driven by progressive genetic and epigenetic alterations, enabling unrestricted growth and expansion. This study provides insights into the hierarchical nature of tumor evolution through tracking phylogenetic relationships between cancer cells, allowing for in-depth studies of tumor progression.
Editorial Material
Oncology
Eunmi Lee, Yibin Kang
Summary: The combination of single-cell lineage tracing and RNA sequencing has opened up unprecedented opportunities for prospectively exploring metastatic dynamics. In a pancreatic cancer model, Simeonov et al. developed the macsGESTALT lineage recording system, revealing that hybrid EMT states and S100 expression are associated with increased metastatic abilities.
Review
Cell Biology
Sadie VanHorn, Samantha A. Morris
Summary: Lineage tracing and fate mapping are two disciplines for tracking cells and their progeny, with lineage tracing focusing on identifying progeny and fate maps showing embryo development. Recent genomic technologies have enabled high-resolution reconstruction of lineage relationships. Fate maps, however, offer spatial information often lost in lineage reconstruction.
DEVELOPMENTAL CELL
(2021)
Review
Biochemistry & Molecular Biology
Mingze Yao, Tinglin Ren, Yuanqing Pan, Xiaoqing Xue, Rong Li, Lei Zhang, Yuhang Li, Ke Huang
Summary: This review presents the progress of the latest CbLT (CRISPR-based Lineage Tracing) and discusses the current limitations and potential technical pitfalls in their application and other emerging concepts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)