Article
Chemistry, Analytical
Dong Xu, Xijie Xu, Zhenqiang Fan, Meifen Zou, Xiaofeng Qin, Yuedi Ding, Ying Peng, Kai Zhang
Summary: A simple, sensitive, and specific fluorescence-based strategy for detecting transcription factors (TFs) was developed by combining molecular beacon (MB) with exonuclease III (Exo III). This method is sensitive, expandable, and suitable for the direct detection of TFs in cancer cell nuclear extracts.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Huali Jin, Ruike Liu, Tian Bai, Min Wei, Baoshan He, Zhiguang Suo
Summary: A low-noise ratiometric fluorescence biosensor based on Pb2+-dependent DNAzyme and exonuclease III (Exo III)-assisted cascade signal amplification was developed for the detection of lead ions. The biosensor exhibited a lower limit of detection and a wider detection range compared to other methods, and showed satisfactory results in the detection of lead ions in tap water, tea, and rice flour samples. This ratiometric fluorescence biosensor has great potential in various target monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Di Zhang, Xin Yu, Lina Wu, Huali Jin, Min Wei
Summary: A sensitive and selective electrochemical Pb2+ sensor was developed based on DNAzyme and exonuclease III, allowing for a wide detection range and high sensitivity in detecting Pb2+ concentrations through target recycling amplification.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Bingzhi Li, Zicheng Shao, Yue Chen
Summary: A CRISPR-based biosensor was developed for measuring TFs levels, detecting NF-kappa B p50 subunit, and potential applications in screening TFs inhibitors. The method showed high sensitivity, low cost, and good portability, enriching the arsenal for TFs-related applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Fang Deng, Jiafeng Pan, Zhi Liu, Junhua Chen
Summary: In this study, we successfully constructed several molecular logic gates using different antibiotics as inputs. The logic system utilizes aptamer-antibiotic recognition and DNAzyme catalysis reaction to detect fluorescence signals. The constructed logic gate system exhibits high selectivity and provides a new analytical method for intelligent detection of antibiotics.
Article
Biochemical Research Methods
Jiafeng Pan, Fang Deng, Lingwen Zeng, Zhi Liu, Junhua Chen
Summary: Based on aptamer recognition and target-mediated competitive hybridization of hairpin probes, a fluorescence sensor for kanamycin (KAN) detection has been developed. The sensor exhibits the advantages of simple operation, high sensitivity, and good robustness, and has been applied to the detection of KAN in milk and water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Immunology
Nadia Jeremiah, Hermine Ferran, Konstantina Antoniadou, Kevin De Azevedo, Jovan Nikolic, Mathieu Maurin, Philippe Benaroch, Nicolas Manel
Summary: A transcriptional rheostat orchestrated by RELA controls the expression of type I/III interferon in T cells, conferring them with innate-like abilities. Despite intact cGAS-STING signaling, CD4(+) T cells exhibit weaker IFN-I/III responses compared to dendritic cells or macrophages. The lysine residues in RELA regulate the IFN-I/III response in CD4(+) T cells at baseline and in response to STING stimulation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Food Science & Technology
Xiaoyi Ma, Cang Wang, Mingcan Qin, Xiaopeng Tian, Shihui Fan, Hangtian Zu, Mingsheng Lyu, Shujun Wang
Summary: A DNAzyme targeting Aeromonas hydrophila was developed through in vitro selection, and a fluorescent biosensor was designed for detecting the bacteria efficiently. The biosensor showed stable performance for more than 6 months and was validated for detecting the pathogen in food samples, demonstrating its potential for use in preventing and detecting foodborne pathogens in the food industry.
Article
Chemistry, Physical
Baiqiang Zhai, Kunlun Huang, Hongtao Wang, Dongmin Su, Yuancong Xu
Summary: A dual-DNAzyme colorimetric biosensor was developed using Cu-DNAzyme and G4-DNAzyme for the detection of Cu2+ through exonuclease III-mediated cyclical assembly. By adjusting metal-dependent DNA-cleaving DNAzymes, this biosensor can detect any metal ion target, which is significant for rapid determination of food safety.
Article
Engineering, Environmental
Sara Ghezelsefloo, Jaber Keyvan Rad, Mahtab Hajiali, Ali Reza Mahdavian
Summary: This study focused on the preparation of fluorescent poly(methyl methacrylate-co-glycidyl methacrylate) nanoparticles bearing Rhodamine B ethylenediamine acrylate for sensing Fe2+ and Fe3+ ions. The results showed that these nanoparticles exhibited immediate color change and enhanced fluorescence emission upon the addition of Fe2+ and Fe3+, with low detection limits and a wide linear detection range. These nanoparticles could potentially be used as colorimetric and fluorescent sensors with high sensitivity and instant responsivity for Fe2+ and Fe3+ ions in future applications.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Evolutionary Biology
Michelle M. Leger, Nuria Ros-Rocher, Sebastian R. Najle, Inaki Ruiz-Trillo
Summary: The Rel/NF-kappa B transcription factor family plays diverse roles in immunity, development, and differentiation in animals. New research suggests that these proteins originated earlier than previously thought, in the last common ancestor of animals and fungi. The proteins in the sister group to fungi also contain elements necessary for subcellular regulation of Rel/NF-kappa B, similar to those found in animals.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Chemistry, Analytical
Dongyou Zhang, Yali Wang, Xiaoyu Jin, Qi Xiao, Shan Huang
Summary: A label-free and ultrasensitive electrochemical biosensor for oral cancer overexpressed 1 (ORAOV1) gene was constructed using exonuclease III-assisted target recycling and dual enzyme-assisted signal amplification strategies. The biosensor can accurately detect ORAOV1 gene in human saliva samples.
Article
Biochemistry & Molecular Biology
Sisi Huang, Kaiyue Wang, Dongyan Huang, Xin Su, Rongfu Yang, Congcong Shao, Juan Jiang, Jianhui Wu
Summary: This study found that lower doses of BPAF can promote dorsal prostatic hyperplasia in rats by interfering with multiple biomarkers in the NF-kappa B signaling pathway. These findings further confirm the toxic effect of BPAF on prostate health and provide important clues to the underlying mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Asiya Kamber Zaidi, Puya Dehgani-Mobaraki
Summary: This article discusses the mechanism of action of Ivermectin against SARS-CoV-2 and its potential in preventing complications, summarizing the available literature on the drug over the years.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Chemistry, Analytical
Xu Chu, Tao Chen, Yan Cao
Summary: This paper reports a simple, rapid and sensitive fluorescence sensor using Carbon Dot (CD) as a probe for the parallel determination of Fe3+, Tb3+ and Eu3+ ions. The CD can selectively quench the fluorescence of Fe3+ through non-radiative electron-transfer and enhance the fluorescence of Tb3+ and Eu3+ through the antenna effect. The fluorescence quenching mechanism and energy transfer mechanism were also studied in details.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Jingjing Ming, Ting-Fu Jiang, Yuan-Hong Wang, Zhi-Hua Lv
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Chemistry, Analytical
Jingjing Ming, Wenbin Fan, Ting-Fu Jiang, Yuan-Hong Wang, Zhi-Hua Lv
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Biophysics
Shufeng Liu, Ying Wang, Jingjing Ming, Ying Lin, Chuanbin Cheng, Feng Li
BIOSENSORS & BIOELECTRONICS
(2013)
Article
Biophysics
Shufeng Liu, Jingjing Ming, Ying Lin, Chunfeng Wang, Chuanbin Cheng, Tao Liu, Li Wang
BIOSENSORS & BIOELECTRONICS
(2014)
Article
Chemistry, Multidisciplinary
Shufeng Liu, Chengxin Zhang, Jingjing Ming, Chunfeng Wang, Tao Liu, Feng Li
CHEMICAL COMMUNICATIONS
(2013)
Article
Chemistry, Analytical
Shufeng Liu, Jingjing Ming, Ying Lin, Chunfeng Wang, Tao Liu, Chuanbin Cheng, Feng Li
SENSORS AND ACTUATORS B-CHEMICAL
(2014)
Article
Chemistry, Multidisciplinary
Li-Ya Jiang, Jing-Jing Ming, Lian-Yue Wang, Yuan-Yuan Jiang, Lan-Hui Ren, Zi-Cheng Wang, Wen-Chen Cheng
Article
Chemistry, Analytical
Shue Deng, Baolong Zhou, Wenjing Li, Hui Li, Fenglian Zhang, Jingjing Ming
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)