期刊
BIOSENSORS & BIOELECTRONICS
卷 49, 期 -, 页码 472-477出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.05.037
关键词
Hybridization chain reaction; DNA hybridization; Electrochemical biosensor; Target catalyzed hairpin assembly; Two-step signal amplification
类别
资金
- National Natural Science Foundation of China [21175076, 21005043]
- Scientific Special Expenditure for Non-profit Public Industry of State Oceanic Administration [201105020]
- Basic Research Program of Qingdao [11-2-4-3-(4)-jch]
- Science Foundation of China Postdoctor [2012M511537]
An isothermal, enzyme-free and ultrasensitive protocol for electrochemical detection of DNA is proposed based on the ingenious combination of target catalyzed hairpin assembly and hybridization chain reaction (HCR) strategies for two-step signal amplification. The DNA hairpin assembly on the electrode is triggered by target DNA, accompanied by the release of target DNA for the successive hybridization and assembly process. The newly emerging DNA fragment on the electrode after hairpin assembly is further used to propagate the HCR between methylene blue-labeled signal probe and auxiliary probe, inducing a remarkably amplified electrochemical signal. The current dual signal amplification strategy is relatively simple and inexpensive owing to avoid the use of any kind of enzyme or sophisticated equipment. It can achieve a sensitivity of 0.1 fM with a wide linear dynamic range from 1 x 10(-15) to 1 x 10(-10) M and discriminate mismatched DNA from perfect matched target DNA with a high selectivity. The high sensitivity and selectivity make this method a great potential for early diagnosis in gene-related diseases. (C) 2013 Elsevier B.V. All rights reserved.
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