期刊
SCIENTIA HORTICULTURAE
卷 201, 期 -, 页码 10-17出版社
ELSEVIER
DOI: 10.1016/j.scienta.2016.01.022
关键词
Axillary buds; Multiplication; GA(3); Rooting; Polyamines; Glomus mosseae
类别
资金
- National Science and Technology Support Program of China [2012BAD01B07]
Tree peony (Paeonia sect. Moutan) is a famous ornamental crop worldwide that is difficult to propagate. The present study provides efficient protocols for the micropropagation of three economically important tree peony cultivars (Paeonia suffruticosa 'Jin Pao Hong', P. suffruticosa 'Wu Long Peng Sheng', and P. x lemoinei 'High Noon'). For optimal shoot multiplication, different concentrations of GA(3) were added into woody plant medium (WPM) (double strength Ca2+) containing 1.0 mgl(-1) BAP; 0.5 mgl(-1) GA3 yielded the highest multiplication rate with more than 4 shoots per explant for the three cultivars. For better rooting, different polyamines (putrescine, spermine, or spermidine) were added individually to half strength MS medium containing 1.0 mgl(-1) IBA. 5.0, 1.0, and 1.0 mgl(-1) putrescine produced the optimal rooting for lin Pao Hong' (100%), 'Wu Long Peng Sheng' (100%), and 'High Noon' (80%), respectively; each rooted shoot had 4-6 roots measuring 4-6 cm in length. Poor survival rates during acclimatization were obtained for lin Pao Hong' (52.6%), 'Wu Long Peng Sheng' (40.1%), and 'High Noon' (64.0%) when the plantlets were grown in uninoculated vermiculite/peat/perlite sterilized substrate; in contrast, 'High Noon' plantlets inoculated with Glomus mosseae showed a high survival rate (83.4%) and vigorous growth. The protocols reported in this study can be used for rapid and large-scale propagation of the three tree peony cultivars. Furthermore, this study raises interesting possibilities for stimulating the in vitro rooting of tree peony cultivars through polyamine application and for improving the ex vitro establishment of tree peony plantlets through arbuscular mycorrhizal fungi inoculation. (C) 2016 Elsevier B.V. All rights reserved.
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