Article
Cell Biology
Huan Niu, Meng Zhao, Jing Huang, Jing Wang, Yang Si, Shan Cheng, Wei Ding
Summary: CBs may serve as indicators of drug sensitivity or resistance in tumor cells, while UHMK1 maintains the structure and morphology of CBs by regulating splicing events.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Cell Biology
Madelyn K. Logan, Katheryn E. Lett, Michael D. Hebert
Summary: The study demonstrates an increase in CB number and upregulation of CB marker protein coilin in WI-38 cells under hypoxia, suggesting a link to dysregulated miRNA expression.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biology
Graham J. M. Hickey, Candice L. Wike, Xichen Nie, Yixuan Guo, Mengyao Tan, Patrick J. Murphy, Bradley R. Cairns
Summary: This study reveals the order and mechanism of establishing developmental gene poising in zebrafish embryos, highlighting the critical role of H2Aub1.
Article
Biology
Madelyn K. Logan, Katheryn E. Lett, Douglas M. McLaurin, Michael D. Hebert
Summary: The NF-kappa B pathway is an important regulator of inflammatory signaling, and aberrant activation is associated with various disorders, including preeclampsia. This study identifies coilin as a novel regulator of NF-kappa B activation and sFlt-1 upregulation.
Article
Biochemistry & Molecular Biology
Hidefumi Suzuki, Kazuki Furugori, Ryota Abe, Shintaro Ogawa, Sayaka Ito, Tomohiko Akiyama, Keiko Horiuchi, Hidehisa Takahashi
Summary: Mediator is a coregulatory complex that plays essential roles in multiple processes of transcription regulation. One of the subunits of Mediator, MED26, is involved in recruiting the super elongation complex (SEC) and the little elongation complex (LEC) to different types of genes. It has been found that MED26-containing Mediator is involved in the regulation of 3' Pol II pausing and 3'-end processing of replication-dependent histone genes by recruiting Cajal bodies (CBs) to histone locus bodies (HLBs). This suggests that Mediator may be involved in the organization of nuclear bodies to coordinate various processes of gene transcription.
Article
Microbiology
Laura White, Bilgi Erbay, G. Eric Blair
Summary: Cajal bodies can be reorganized into microfoci during adenovirus infection. The protein p80-coilin forms a complex with L4-22K protein, facilitating the nuclear export of Ad mRNA.
Editorial Material
Biology
De-Li Shi
Summary: During oogenesis, maternal gene products play essential roles in early developmental processes. However, loss of their zygotic functions often leads to lethality or sterility, preventing the generation of maternal mutants. A recently developed oocyte-specific knockout strategy in zebrafish allows for the elimination of maternal gene products in one fish generation, providing a more accessible method for studying maternal-effect genes.
Article
Biology
Jeff Jiajing Zhou, Jin Sun Cho, Han Han, Ira L. Blitz, Wenqi Wang, Ken W. Y. Cho
Summary: Histone acetylation is a crucial epigenetic modification that regulates gene expression. Histone deacetylase 1 (Hdac1) binds to the zygotic genome during early embryogenesis and plays a dual role in both repressing and maintaining gene expression through histone acetylation-deacetylation cycles.
Article
Multidisciplinary Sciences
Claire Armstrong, Sabrina L. Spencer
Summary: The study revealed a hybrid model in which RD histone biosynthesis is initially initiated in G1, followed by an increase in histone production in S phase, indicating different mechanisms of histone synthesis in different stages of the cell cycle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Xin Liu, Jingjing Zhang, Jilong Zhou, Guowei Bu, Wei Zhu, Hainan He, Qiaoran Sun, Zhisheng Yu, Wenjing Xiong, Liyan Wang, Danya Wu, Chengli Dou, Longtao Yu, Kai Zhou, Shangke Wang, Zhengang Fan, Tingting Wang, Ruifeng Hu, Taotao Hu, Xia Zhang, Yi-Liang Miao
Summary: This study reveals the genome-wide distribution of histone variant H2A.Z in mouse oocytes and early embryos, and demonstrates its role in regulating gene expression and histone modifications during preimplantation development. The study also shows that H2A.Z exhibits different accumulation patterns at promoters, indicating its involvement in transcriptional activation, gene suppression, and gene silencing. Depletion of H2A.Z leads to abnormal gene expression and developmental arrest.
Article
Genetics & Heredity
Prachi N. Ghule, Joseph R. Boyd, Fleur Kabala, Andrew J. Fritz, Nicole A. Bouffard, Cong Gao, Kathleen Bright, Jill Macfarlane, David J. Seward, Gianluca Pegoraro, Tom Misteli, Jane B. Lian, Seth Frietze, Janet L. Stein, Andre J. van Wijnen, Gary S. Stein
Summary: Human Histone Locus Bodies (HLBs) are nuclear subdomains consisting of clustered histone genes that undergo coordinated regulation. Changes in the spatial organization of chromatin at HLBs during the G1 phase of the cell cycle were studied using MCF10 breast cancer progression model cell lines. The study revealed the localization of histone gene regulatory proteins HINFP and NPAT at specific anchoring points in chromatin loops, suggesting their role in histone biosynthesis for packaging newly replicated DNA. Additionally, a novel enhancer region located distal to histone gene sub-clusters on chromosome 6 was identified, which makes consistent genomic contacts with HLB chromatin and is bound by NPAT. The findings support a model in which the HINFP/NPAT complex controls the dynamic remodeling of higher-order genomic organization at HLBs to support histone mRNA transcription during the G1 phase.
Article
Reproductive Biology
Mingtian Deng, Baobao Chen, Yingnan Yang, Yongjie Wan, Zifei Liu, Jun Fu, Feng Wang
Summary: This study utilized RNA sequencing to analyze the transcriptional activity of SCNT embryos during ZGA/EGA in goats, mice, humans, and cattle. The research identified differentially expressed genes that failed to be reprogrammed during SCNT embryo development, as well as key players in histone methylation and N6-methyladenosine modifiers that were differentially expressed in SCNT embryos of all species. Furthermore, the study identified critical genes potentially essential for SCNT reprogramming using weighted gene correlation network analysis, providing valuable insights for future studies.
BIOLOGY OF REPRODUCTION
(2021)
Review
Plant Sciences
Zhen Lei, Ling Wang, Eun Yu Kim, Jungnam Cho
Summary: Gene expression can be modulated by epigenetic mechanisms, including liquid-liquid phase separation (LLPS) in plants, which plays a key role in the chromatin and small RNA pathways. LLPS provides a mechanistic explanation for the self-assembly of subcellular structures, contributing to the efficiency and specificity of certain cellular reactions.
Review
Materials Science, Multidisciplinary
Reed E. S. Harrison, Kegui Weng, Yingxiao Wang, Qin Peng
Summary: Liquid-liquid phase separation is increasingly recognized as a phenomenon that affects cell behavior, particularly in the phase separation of transcription factors and coactivators. While phase separation of intracellular components has been observed for many years, only recently have researchers been able to understand the functional significance of these events. By studying the phase separation of chromatin in a histone-dependent manner, researchers can regulate the genome and control cell behavior.
CURRENT OPINION IN SOLID STATE & MATERIALS SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Rong Sang, Cheng Wu, Shanshan Xie, Xiao Xu, Yuhan Lou, Wanzhong Ge, Yongmei Xi, Xiaohang Yang
Summary: The study demonstrates that the Mxc gene plays a critical role in the development of the nervous system in Drosophila. Silencing of Mxc leads to a massive loss of neurons and locomotion dysfunction in adult flies. Additionally, Mxc knockdown results in premature termination of neural stem cell fate and inhibition of ganglion mother cell differentiation.
CELL AND BIOSCIENCE
(2022)
Article
Multidisciplinary Sciences
Marissa J. Schafer, Thomas A. White, Koji Iijima, Andrew J. Haak, Giovanni Ligresti, Elizabeth J. Atkinson, Ann L. Oberg, Jodie Birch, Hanna Salmonowicz, Yi Zhu, Daniel L. Mazula, Robert W. Brooks, Heike Fuhrmann-Stroissnigg, Tamar Pirtskhalava, Y. S. Prakash, Tamara Tchkonia, Paul D. Robbins, Marie Christine Aubry, Joao F. Passos, James L. Kirkland, Daniel J. Tschumperlin, Hirohito Kita, Nathan K. LeBrasseur
NATURE COMMUNICATIONS
(2017)
Review
Cell Biology
Mikolaj Ogrodnik, Hanna Salmonowicz, Vadim N. Gladyshev
Article
Cell Biology
Jonathan Rodenfels, Karla M. Neugebauer, Jonathon Howard
DEVELOPMENTAL CELL
(2019)
Article
Biochemistry & Molecular Biology
Rhys Anderson, Anthony Lagnado, Damien Maggiorani, Anna Walaszczyk, Emily Dookun, James Chapman, Jodie Birch, Hanna Salmonowicz, Mikolaj Ogrodnik, Diana Jurk, Carole Proctor, Clara Correia-Melo, Stella Victorelli, Edward Fielder, Rolando Berlinguer-Palmini, Andrew Owens, Laura C. Greaves, Kathy L. Kolsky, Angelo Parini, Victorine Douin-Echinard, Nathan K. Lebrasseur, Helen M. Arthur, Simon Tual-Chalot, Marissa J. Schafer, Carolyn M. Roos, Jordan D. Miller, Neil Robertson, Jelena Mann, Peter D. Adams, Tamara Tchkonia, James L. Kirkland, Jeanne Mialet-Perez, Gavin D. Richardson, Joao F. Passos
Review
Biochemistry & Molecular Biology
Leena Habiballa, Hanna Salmonowicz, Joao F. Passos
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Review
Biochemistry & Molecular Biology
Mikolaj Ogrodnik, Hanna Salmonowicz, Diana Jurk, Joao F. Passos
TRENDS IN BIOCHEMICAL SCIENCES
(2019)
Article
Cell Biology
Jonathan Rodenfels, Pablo Sartori, Stefan Golfier, Kartikeya Nagendra, Karla M. Neugebauer, Jonathon Howard
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Cell Biology
Mikolaj Ogrodnik, Shane A. Evans, Edward Fielder, Stella Victorelli, Patrick Kruger, Hanna Salmonowicz, Bettina M. Weigand, Ayush D. Patel, Tamar Pirtskhalava, Christine L. Inman, Kurt O. Johnson, Stephanie L. Dickinson, Azucena Rocha, Marissa J. Schafer, Yi Zhu, David B. Allison, Thomas von Zglinicki, Nathan K. LeBrasseur, Tamar Tchkonia, Nicola Neretti, Joao F. Passos, James L. Kirkland, Diana Jurk
Summary: Clearing senescent cells, either through drug treatment or senolytic cocktail, has been shown to improve cognitive function, providing proof-of-concept for senolytic interventions as a potential therapeutic avenue for alleviating age-associated cognitive impairment.
Review
Developmental Biology
Wilhelm Palm, Jonathan Rodenfels
Article
Biochemistry & Molecular Biology
Anthony Lagnado, Jack Leslie, Marie-Helene Ruchaud-Sparagano, Stella Victorelli, Petra Hirsova, Mikolaj Ogrodnik, Amy L. Collins, Maria Grazia Vizioli, Leena Habiballa, Gabriele Saretzki, Shane A. Evans, Hanna Salmonowicz, Adam Hruby, Daniel Geh, Kevin D. Pavelko, David Dolan, Helen L. Reeves, Sushma Grellscheid, Colin H. Wilson, Sanjay Pandanaboyana, Madison Doolittle, Thomas von Zglinicki, Fiona Oakley, Suchira Gallage, Caroline L. Wilson, Jodie Birch, Bernadette Carroll, James Chapman, Mathias Heikenwalder, Nicola Neretti, Sundeep Khosla, Claudio Akio Masuda, Tamar Tchkonia, James L. Kirkland, Diana Jurk, Derek A. Mann, Joao F. Passos
Summary: The research shows that neutrophils cause oxidative damage to telomeres in non-immune cells, promoting cellular senescence. In aged liver, senescent cells recruit neutrophils, potentially leading to the spread of senescence.
Review
Biochemistry & Molecular Biology
Karl N. Miller, Stella G. Victorelli, Hanna Salmonowicz, Nirmalya Dasgupta, Tianhui Liu, Joao F. Passos, Peter D. Adams
Summary: This article summarizes the key role of endogenous cytoplasmic DNA (cytoDNA) species in inflammation, emphasizing the similarities and differences between different cytoDNAs, as well as their underlying molecular mechanisms of formation and function.
Article
Biochemistry & Molecular Biology
Karolina Labedzka-Dmoch, Michal Razew, Marta Gapinska, Jakub Piatkowski, Adam Kolondra, Hanna Salmonowicz, Joanna M. Wenda, Marcin Nowotny, Pawel Golik
Summary: Pet127 is a mitochondrial protein with conserved presence and function in different eukaryotes. It is classified as a member of the PD-(D/E)XK superfamily and exhibits exoribonuclease activity. Deletion of the PET127 gene in pathogenic yeast leads to abnormal increase in transcript levels, indicating the important role of Pet127 protein in RNA degradation.
Article
Cell Biology
Matthew D. W. Piper, George A. Soultoukis, Eric Blanc, Andrea Mesaros, Samantha L. Herbert, Paula Juricic, Xiaoli He, Ilian Atanassov, Hanna Salmonowicz, Mingyao Yang, Stephen J. Simpson, Carlos Ribeiro, Linda Partridge