Synthetic biology for production of natural and new-to-nature terpenoids in photosynthetic organisms

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

(2016) Johan Andersen-Ranberg et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Production of oleanane-type sapogenin in transgenic rice via expression of β-amyrin synthase gene from Panax japonicus C. A. Mey

(2015) Zhiwei Huang et al.   BMC BIOTECHNOLOGY

The increasing value of plant-made proteins

(2015) Markus Sack et al.   CURRENT OPINION IN BIOTECHNOLOGY

Reconstructing the chemical diversity of labdane-type diterpene biosynthesis in yeast

(2015) Codruta Ignea et al.   METABOLIC ENGINEERING

Production of bioactive ginsenosides Rh2 and Rg3 by metabolically engineered yeasts

(2015) Pingping Wang et al.   METABOLIC ENGINEERING

Unraveling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa lanceolata

(2015) Tessa Moses et al.   Molecular Plant

It Is Easy to Get Huge Candidate Gene Lists for Plant Metabolism Now, but How to Get Beyond?

(2015) Alain Goossens   Molecular Plant

Standards for plant synthetic biology: a common syntax for exchange of DNA parts

(2015) Nicola J. Patron et al.   NEW PHYTOLOGIST

Production of carotenoids by microalgae: achievements and challenges

(2015) João C. Varela et al.   PHOTOSYNTHESIS RESEARCH

P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins

(2015) Hikaru Seki et al.   PLANT AND CELL PHYSIOLOGY

Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor-product balance

(2015) Chao Bai et al.   PLANT BIOTECHNOLOGY JOURNAL

Photosynthetic terpene hydrocarbon production for fuels and chemicals

(2015) Xin Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis

(2015) Xi-Wu Pan et al.   Plant Biotechnology Reports

OSC2 and CYP716A14v2 Catalyze the Biosynthesis of Triterpenoids for the Cuticle of Aerial Organs ofArtemisia annua

(2015) Tessa Moses et al.   PLANT CELL

Exploring diterpene metabolism in non-model species: transcriptome-enabled discovery and functional characterization of labda-7,13E-dienyl diphosphate synthase fromGrindelia robusta

(2015) Philipp Zerbe et al.   PLANT JOURNAL

Identification and genome organization of saponin pathway genes from a wild crucifer, and their use for transient production of saponins inNicotiana benthamiana

(2015) Bekzod Khakimov et al.   PLANT JOURNAL

Production of dammarane-type sapogenins in rice by expressing the dammarenediol-II synthase gene from Panax ginseng C.A. Mey

(2015) Zhiwei Huang et al.   PLANT SCIENCE

Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

(2015) Philipp Zerbe et al.   TRENDS IN BIOTECHNOLOGY

Enabling plant synthetic biology through genome engineering

(2015) Nicholas J. Baltes et al.   TRENDS IN BIOTECHNOLOGY

Metabolic engineering of cyanobacteria for the synthesis of commodity products

(2015) S. Andreas Angermayr et al.   TRENDS IN BIOTECHNOLOGY

Stable heterologous expression of biologically active terpenoids in green plant cells

(2015) N. Kusaira B. K. Ikram et al.   Frontiers in Plant Science

Towards Elucidating Carnosic Acid Biosynthesis in Lamiaceae: Functional Characterization of the Three First Steps of the Pathway in Salvia fruticosa and Rosmarinus officinalis

(2015) Dragana Božić et al.   PLoS One

Novel Product Chemistry from Mechanistic Analysis ofent-Copalyl Diphosphate Synthases from Plant Hormone Biosynthesis

(2014) Kevin Potter et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering Complex Metabolic Pathways in Plants

(2014) Gemma Farré et al.   Annual Review of Plant Biology

Triterpene Biosynthesis in Plants

(2014) Ramesha Thimmappa et al.   Annual Review of Plant Biology

Techno-Economic Analysis of Horseradish Peroxidase Production Using a Transient Expression System in Nicotiana benthamiana

(2014) David Richard Walwyn et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Metabolic engineering for the microbial production of carotenoids and related products with a focus on the rare C50 carotenoids

(2014) Sabine A. E. Heider et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genetically engineering cyanobacteria to convert CO2, water, and light into the long-chain hydrocarbon farnesene

(2014) Charles Halfmann et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A rational approach to improving the biotechnological production of taxanes in plant cell cultures of Taxus spp.

(2014) Rosa M. Cusido et al.   BIOTECHNOLOGY ADVANCES

Xylose fermentation as a challenge for commercialization of lignocellulosic fuels and chemicals

(2014) Violeta Sànchez Nogué et al.   BIOTECHNOLOGY LETTERS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Production of bioactive ginsenoside compound K in metabolically engineered yeast

(2014) Xing Yan et al.   CELL RESEARCH

Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives

(2014) Tessa Moses et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Transient expressions of synthetic biology in plants

(2014) Frank Sainsbury et al.   CURRENT OPINION IN PLANT BIOLOGY

Plant carotenoids: genomics meets multi-gene engineering

(2014) Giovanni Giuliano   CURRENT OPINION IN PLANT BIOLOGY

Engineering of cyanobacteria for the photosynthetic production of limonene from CO2

(2014) Hiroshi Kiyota et al.   JOURNAL OF BIOTECHNOLOGY

Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway

(2014) Anneli Ritala et al.   JOURNAL OF BIOTECHNOLOGY

Toward a photosynthetic microbial platform for terpenoid engineering

(2014) Fiona K. Davies et al.   PHOTOSYNTHESIS RESEARCH

A novel carotenoid, 4-keto-α-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus

(2014) Jürgen Breitenbach et al.   PHYTOCHEMISTRY

Characterization of two genes for the biosynthesis of abietane-type diterpenes in rosemary (Rosmarinus officinalis) glandular trichomes

(2014) Kathleen Brückner et al.   PHYTOCHEMISTRY

Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato

(2014) S. Sawai et al.   PLANT CELL

Metabolic engineering of volatile isoprenoids in plants and microbes

(2014) CLAUDIA E. VICKERS et al.   PLANT CELL AND ENVIRONMENT

Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene

(2014) Md. Mohidul Hasan et al.   PLANT CELL REPORTS

Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway

(2014) Kaouthar Eljounaidi et al.   PLANT SCIENCE

Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli

(2014) Miho Takemura et al.   PLANTA

Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants

(2014) Cinzia Formighieri et al.   PLANTA

Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16  hydroxylase from Bupleurum falcatum

(2014) T. Moses et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Key Applications of Plant Metabolic Engineering

(2014) Warren Lau et al.   PLOS BIOLOGY

Metabolic engineering of the moss Physcomitrella patens to produce the sesquiterpenoids patchoulol and α/β-santalene

(2014) Xin Zhan et al.   Frontiers in Plant Science

Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis

(2013) Eva Vranová et al.   Annual Review of Plant Biology

Unique Biosynthesis of Sesquarterpenes (C35Terpenes)

(2013) Tsutomu SATO   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism

(2013) Soo Rin Kim et al.   BIOTECHNOLOGY ADVANCES

Metabolic engineering of Escherichia coli for the biosynthesis of alpha-pinene

(2013) Jianming Yang et al.   Biotechnology for Biofuels

Advances in microalgae engineering and synthetic biology applications for biofuel production

(2013) Javier A Gimpel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering metabolic pathways in plants by multigene transformation

(2013) Uxue Zorrilla-López et al.   INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY

The Biosynthetic Origin of Irregular Monoterpenes inLavandula

(2013) Zerihun A. Demissie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Constraints to commercialization of algal fuels

(2013) Yusuf Chisti   JOURNAL OF BIOTECHNOLOGY

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

(2013) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Characterization of two geraniol synthases from Valeriana officinalis and Lippia dulcis: Similar activity but difference in subcellular localization

(2013) Lemeng Dong et al.   METABOLIC ENGINEERING

Metabolic engineering of tomato for high-yield production of astaxanthin

(2013) Jun-Chao Huang et al.   METABOLIC ENGINEERING

Geraniol hydroxylase and hydroxygeraniol oxidase activities of the CYP76 family of cytochrome P450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway

(2013) René Höfer et al.   METABOLIC ENGINEERING

Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs

(2013) Qiwei Shan et al.   Molecular Plant

Sesterterpenoids

(2013) Lishu Wang et al.   NATURAL PRODUCT REPORTS

The protein quality control system manages plant defence compound synthesis

(2013) Jacob Pollier et al.   NATURE

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Malaria drug made in yeast causes market ferment

(2013) Mark Peplow   NATURE

Synergistic and tunable human gene activation by combinations of synthetic transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Bioengineering of plant (tri)terpenoids: from metabolic engineering of plants to synthetic biologyin vivoandin vitro

(2013) Tessa Moses et al.   NEW PHYTOLOGIST

Plant P450s as versatile drivers for evolution of species-specific chemical diversity

(2013) B. Hamberger et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Cytochrome P450-enzymes involved in the biosynthesis of mono- and sesquiterpenes

(2013) Corinna Weitzel et al.   PHYTOCHEMISTRY REVIEWS

The Involvement of  -Amyrin 28-Oxidase (CYP716A52v2) in Oleanane-Type Ginsenoside Biosynthesis in Panax ginseng

(2013) J.-Y. Han et al.   PLANT AND CELL PHYSIOLOGY

Chrysanthemum expressing a linalool synthase gene ‘smells good’, but ‘tastes bad’ to western flower thrips

(2013) Ting Yang et al.   PLANT BIOTECHNOLOGY JOURNAL

The Plant Science Decadal Vision

(2013) C. Martin   PLANT CELL

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

Anin vitrosystem for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation

(2013) Chao Bai et al.   PLANT JOURNAL

High-level diterpene production by transient expression in Nicotiana benthamiana

(2013) Kathleen Brückner et al.   Plant Methods

TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants

(2013) Toni Wendt et al.   PLANT MOLECULAR BIOLOGY

GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology

(2013) A. Sarrion-Perdigones et al.   PLANT PHYSIOLOGY

Post-translational events and modifications regulating plant enzymes involved in isoprenoid precursor biosynthesis

(2013) Andréa Hemmerlin   PLANT SCIENCE

Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

(2012) Mi-Jeong Cha et al.   BMB Reports

Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries

(2012) Kuk-Ki Hong et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems

(2012) Marc J.C. Fischer et al.   JOURNAL OF BIOTECHNOLOGY

A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010

(2012) Stephen S Lim et al.   LANCET

Structure and Dynamics of the Isoprenoid Pathway Network

(2012) Eva Vranová et al.   Molecular Plant

Exploiting diversity and synthetic biology for the production of algal biofuels

(2012) D. Ryan Georgianna et al.   NATURE

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

Functional characterization of wheat ent-kaurene(-like) synthases indicates continuing evolution of labdane-related diterpenoid metabolism in the cereals

(2012) Ke Zhou et al.   PHYTOCHEMISTRY

Cytochrome P450 CYP716A53v2 Catalyzes the Formation of Protopanaxatriol from Protopanaxadiol During Ginsenoside Biosynthesis in Panax Ginseng

(2012) Jung-Yeon Han et al.   PLANT AND CELL PHYSIOLOGY

Metabolic engineering of plant monoterpenes, sesquiterpenes and diterpenes-current status and future opportunities

(2012) B. Markus Lange et al.   PLANT BIOTECHNOLOGY JOURNAL

The tomatocis-prenyltransferase gene family

(2012) Tariq A. Akhtar et al.   PLANT JOURNAL

UDP-Glycosyltransferases from the UGT73C Subfamily in Barbarea vulgaris Catalyze Sapogenin 3-O-Glucosylation in Saponin-Mediated Insect Resistance

(2012) J. M. Augustin et al.   PLANT PHYSIOLOGY

Molecular characterization of the pentacyclic triterpenoid biosynthetic pathway in Catharanthus roseus

(2012) Lili Huang et al.   PLANTA

Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression

(2012) Mi-Jin Kim et al.   PLoS One

Application of synthetic biology in cyanobacteria and algae

(2012) Bo Wang et al.   Frontiers in Microbiology

Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals

(2011) Dana Morrone et al.   BIOCHEMICAL JOURNAL

Artemisinin: Discovery from the Chinese Herbal Garden

(2011) Louis H. Miller et al.   CELL

Biosynthesis of Cholesterol and Other Sterols

(2011) W. David Nes   CHEMICAL REVIEWS

Metabolic engineering of microbial pathways for advanced biofuels production

(2011) Fuzhong Zhang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microalgae as Sources of Carotenoids

(2011) Ana Catarina Guedes et al.   Marine Drugs

Metabolic engineering of geranic acid in maize to achieve fungal resistance is compromised by novel glycosylation patterns

(2011) Ting Yang et al.   METABOLIC ENGINEERING

Combinatorial biosynthesis in plants: A (p)review on its potential and future exploitation

(2011) Jacob Pollier et al.   NATURAL PRODUCT REPORTS

Generation of the potent anti-malarial drug artemisinin in tobacco

(2011) Moran Farhi et al.   NATURE BIOTECHNOLOGY

Molecular activities, biosynthesis and evolution of triterpenoid saponins

(2011) Jörg M. Augustin et al.   PHYTOCHEMISTRY

The Cyt P450 Enzyme CYP716A47 Catalyzes the Formation of Protopanaxadiol from Dammarenediol-II During Ginsenoside Biosynthesis in Panax ginseng

(2011) Jung-Yeon Han et al.   PLANT AND CELL PHYSIOLOGY

CYP716A Subfamily Members are Multifunctional Oxidases in Triterpenoid Biosynthesis

(2011) Ery O. Fukushima et al.   PLANT AND CELL PHYSIOLOGY

Medicago truncatulaCYP716A12 Is a Multifunctional Oxidase Involved in the Biosynthesis of Hemolytic Saponins

(2011) Maria Carelli et al.   PLANT CELL

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom

(2011) Feng Chen et al.   PLANT JOURNAL

Reconstitution of the Costunolide Biosynthetic Pathway in Yeast and Nicotiana benthamiana

(2011) Qing Liu et al.   PLoS One

A raison d’être for two distinct pathways in the early steps of plant isoprenoid biosynthesis?

(2011) Andréa Hemmerlin et al.   PROGRESS IN LIPID RESEARCH

Triterpenoid Biosynthesis and Engineering in Plants

(2011) Satoru Sawai et al.   Frontiers in Plant Science

Supply of precursors for carotenoid biosynthesis in plants

(2010) Manuel Rodríguez-Concepción   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

The production of the sesquiterpene β-caryophyllene in a transgenic strain of the cyanobacterium Synechocystis

(2010) Robert E. Reinsvold et al.   JOURNAL OF PLANT PHYSIOLOGY

Two rings in them all: The labdane-related diterpenoids

(2010) Reuben J. Peters   NATURAL PRODUCT REPORTS

Integrating carbon–halogen bond formation into medicinal plant metabolism

(2010) Weerawat Runguphan et al.   NATURE

Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm

(2010) Sun-Hwa Ha et al.   PLANT BIOTECHNOLOGY JOURNAL

The production of artemisinin precursors in tobacco

(2010) Yansheng Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Genomic and Coexpression Analyses Predict Multiple Genes Involved in Triterpene Saponin Biosynthesis in Medicago truncatula

(2010) M. A. Naoumkina et al.   PLANT CELL

Nicotiana benthamiana as a Production Platform for Artemisinin Precursors

(2010) Teun W. J. M. van Herpen et al.   PLoS One

Mechanism of action and inhibition of dehydrosqualene synthase

(2010) F.-Y. Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Golden Rice is an effective source of vitamin A

(2009) Guangwen Tang et al.   AMERICAN JOURNAL OF CLINICAL NUTRITION

Diversity, regulation, and genetic manipulation of plant mono- and sesquiterpenoid biosynthesis

(2009) Fengnian Yu et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Genome-wide mapping of alternative splicing in Arabidopsis thaliana

(2009) S. A. Filichkin et al.   GENOME RESEARCH

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

(2009) Hiroko Tsuruta et al.   PLoS One

Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways

(2009) S. Naqvi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens

(2009) Aldwin Anterola et al.   TRANSGENIC RESEARCH

When more is better: multigene engineering in plants

(2009) Shaista Naqvi et al.   TRENDS IN PLANT SCIENCE

Plastid Evolution

(2008) Sven B. Gould et al.   Annual Review of Plant Biology

Aquatic phototrophs: efficient alternatives to land-based crops for biofuels

(2008) G Charles Dismukes et al.   CURRENT OPINION IN BIOTECHNOLOGY

The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin inArtemisia annua

(2008) Yansheng Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production

(2008) Benedikt Engels et al.   METABOLIC ENGINEERING

Terpenoids: Opportunities for Biosynthesis of Natural Product Drugs Using Engineered Microorganisms

(2008) Parayil Kumaran Ajikumar et al.   MOLECULAR PHARMACEUTICS

Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering

(2008) Tomohisa Hasunuma et al.   PLANT JOURNAL

Terpenoid biomaterials

(2008) Jörg Bohlmann et al.   PLANT JOURNAL

Phytochemical diversity: The sounds of silent metabolism

(2008) Efraim Lewinsohn et al.   PLANT SCIENCE

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize

(2008) C. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme β-carotene ketolase CrtW and endogenous carotenoid biosynthetic enzymes in higher plants

(2008) Kazutoshi Shindo et al.   TETRAHEDRON LETTERS

Metabolic engineering of carotenoid biosynthesis in plants

(2008) Giovanni Giuliano et al.   TRENDS IN BIOTECHNOLOGY

Biofuel alternatives to ethanol: pumping the microbial well

(2008) J.L. Fortman et al.   TRENDS IN BIOTECHNOLOGY

Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine

(2007) Óscar Herrero et al.   METABOLIC ENGINEERING