期刊
TRANSGENIC RESEARCH
卷 18, 期 4, 页码 655-660出版社
SPRINGER
DOI: 10.1007/s11248-009-9252-5
关键词
Taxa-4(5),11(12)-diene; Taxadiene synthase; Physcomitrella patens; Taxol; Paclitaxel; Cancer
类别
资金
- Southern Illinois University SEED
- Southern Illinois University REACH Award
Taxadiene synthase gene from Taxus brevifolia was constitutively expressed in the moss Physcomitrella patens using a ubiquitin promoter to produce taxa-4(5),11(12)-diene, the precursor of the anticancer drug paclitaxel. In stable moss transformants, taxa-4(5),11(12)-diene was produced up to 0.05% fresh weight of tissue, without significantly affecting the amounts of the endogenous diterpenoids (ent-kaurene and 16-hydroxykaurane). Unlike higher plants that had been genetically modified to produce taxa-4(5),11(12)-diene, transgenic P. patens did not exhibit growth inhibition due to alteration of diterpenoid metabolic pools. Thus we propose that P. patens is a promising alternative host for the biotechnological production of paclitaxel and its precursors.
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