Cisgenesis and genome editing: combining concepts and efforts for a smarter use of genetic resources in crop breeding

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Plant genome editing by novel tools: TALEN and other sequence specific nucleases

(2015) Thorben Sprink et al.   CURRENT OPINION IN BIOTECHNOLOGY

Editing plant genomes with CRISPR/Cas9

(2015) Khaoula Belhaj et al.   CURRENT OPINION IN BIOTECHNOLOGY

Progress, challenges and the future of crop genomes

(2015) Todd P Michael et al.   CURRENT OPINION IN PLANT BIOLOGY

Targeted gene mutation in tetraploid potato through transient TALEN expression in protoplasts

(2015) Alessandro Nicolia et al.   JOURNAL OF BIOTECHNOLOGY

Gene targeting and editing in crop plants: a new era of precision opportunities

(2015) Amy R. Rinaldo et al.   MOLECULAR BREEDING

Improving resistance of different apple cultivars using the Rvi6 scab resistance gene in a cisgenic approach based on the Flp/FRT recombinase system

(2015) Juliane Würdig et al.   MOLECULAR BREEDING

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennialPopulusreveals 4-coumarate:CoA ligase specificity and redundancy

(2015) Xiaohong Zhou et al.   NEW PHYTOLOGIST

Improving cold storage and processing traits in potato through targeted gene knockout

(2015) Benjamin M. Clasen et al.   PLANT BIOTECHNOLOGY JOURNAL

Creation of fragrant rice by targeted knockout of theOsBADH2gene using TALEN technology

(2015) Qiwei Shan et al.   PLANT BIOTECHNOLOGY JOURNAL

The Regulatory Status of Genome-edited Crops

(2015) Jeffrey D. Wolt et al.   PLANT BIOTECHNOLOGY JOURNAL

Heritable site-specific mutagenesis using TALENs in maize

(2015) Si Nian Char et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

(2015) Shaohui Wang et al.   PLANT CELL REPORTS

Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice

(2015) Junhui Zhou et al.   PLANT JOURNAL

Cas9-Guide RNA Directed Genome Editing in Soybean

(2015) Zhongsen Li et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

CRISPR/Cas9-mediated genome editing and gene replacement in plants: Transitioning from lab to field

(2015) Scott M. Schaeffer et al.   PLANT SCIENCE

Genetic basis and detection of unintended effects in genetically modified crop plants

(2015) Gregory S. Ladics et al.   TRANSGENIC RESEARCH

Enabling plant synthetic biology through genome engineering

(2015) Nicholas J. Baltes et al.   TRENDS IN BIOTECHNOLOGY

Are we ready for back-to-nature crop breeding?

(2015) Michael G. Palmgren et al.   TRENDS IN PLANT SCIENCE

Towards social acceptance of plant breeding by genome editing

(2015) Motoko Araki et al.   TRENDS IN PLANT SCIENCE

Feasibility of new breeding techniques for organic farming

(2015) Martin Marchman Andersen et al.   TRENDS IN PLANT SCIENCE

Targeted mutagenesis in soybean using the CRISPR-Cas9 system

(2015) Xianjun Sun et al.   Scientific Reports

Targeted Modification of Gene Function Exploiting Homology-Directed Repair of TALEN-Mediated Double-Strand Breaks in Barley

(2015) Nagaveni Budhagatapalli et al.   G3-Genes Genomes Genetics

Regulatory uncertainty over genome editing

(2015) Huw D. Jones   Nature Plants

Revisiting GMOs: Are There Differences in European Consumers’ Acceptance and Valuation for Cisgenically vs Transgenically Bred Rice?

(2015) Anne-Cécile Delwaide et al.   PLoS One

Gene Editing by Co-Transformation of TALEN and Chimeric RNA/DNA Oligonucleotides on the Rice OsEPSPS Gene and the Inheritance of Mutations

(2015) Mugui Wang et al.   PLoS One

Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation

(2015) Di Fan et al.   Scientific Reports

Cisgenesis and Intragenesis: New tools For Improving Crops

(2014) C Espinoza et al.   BIOLOGICAL RESEARCH

Development of late blight resistant potatoes by cisgene stacking

(2014) Kwang-Ryong Jo et al.   BMC BIOTECHNOLOGY

The CRISPR–Cas system for plant genome editing: advances and opportunities

(2014) Vinay Kumar et al.   JOURNAL OF EXPERIMENTAL BOTANY

Reply to The slippery slope of cisgenesis

(2014) Henk Schouten   NATURE BIOTECHNOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Genomic analyses provide insights into the history of tomato breeding

(2014) Tao Lin et al.   NATURE GENETICS

Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives

(2014) Magdy M. Mahfouz et al.   PLANT BIOTECHNOLOGY JOURNAL

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

(2014) Hui Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

(2014) William Haun et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato

(2014) S. Sawai et al.   PLANT CELL

CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum

(2014) Junping Gao et al.   PLANT MOLECULAR BIOLOGY

CRISPR–Cas system: a powerful tool for genome engineering

(2014) Liang Liu et al.   PLANT MOLECULAR BIOLOGY

Targeted Mutagenesis of the Tomato PROCERA Gene Using Transcription Activator-Like Effector Nucleases

(2014) V. S. Lor et al.   PLANT PHYSIOLOGY

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Precision genetic modifications: a new era in molecular biology and crop improvement

(2014) Franziska Fichtner et al.   PLANTA

Targeted mutagenesis using zinc-finger nucleases in perennial fruit trees

(2014) Reut Peer et al.   PLANTA

True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells

(2014) Maia Gurushidze et al.   PLoS One

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Harvesting the Promising Fruits of Genomics: Applying Genome Sequencing Technologies to Crop Breeding

(2014) Rajeev K. Varshney et al.   PLOS BIOLOGY

Precision Genome Engineering and Agriculture: Opportunities and Regulatory Challenges

(2014) Daniel F. Voytas et al.   PLOS BIOLOGY

Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding

(2014) Jiangfeng He et al.   Frontiers in Plant Science

Plant Genome Engineering with Sequence-Specific Nucleases

(2013) Daniel F. Voytas   Annual Review of Plant Biology

Whole genome resequencing in tomato reveals variation associated with introgression and breeding events

(2013) Mathilde Causse et al.   BMC GENOMICS

Plant genome sequencing — applications for crop improvement

(2013) Marie E Bolger et al.   CURRENT OPINION IN BIOTECHNOLOGY

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

(2013) Zhen Liang et al.   Journal of Genetics and Genomics

How safe does transgenic food need to be?

(2013) Laura DeFrancesco   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Comparative regulatory approaches for groups of new plant breeding techniques

(2013) Maria Lusser et al.   New Biotechnology

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Molecular characterization of cisgenic lines of apple ‘Gala’ carrying theRvi6scab resistance gene

(2013) Thalia Vanblaere et al.   PLANT BIOTECHNOLOGY JOURNAL

Intragenesis and cisgenesis as alternatives to transgenic crop development

(2013) Inger Baeksted Holme et al.   PLANT BIOTECHNOLOGY JOURNAL

Predictive modelling of complex agronomic and biological systems

(2013) JOOST J. B. KEURENTJES et al.   PLANT CELL AND ENVIRONMENT

Targeted genome modification technologies and their applications in crop improvements

(2013) Kunling Chen et al.   PLANT CELL REPORTS

Barley whole exome capture: a tool for genomic research in the genusHordeumand beyond

(2013) Martin Mascher et al.   PLANT JOURNAL

Whole-exome targeted sequencing of the uncharacterized pine genome

(2013) Leandro G. Neves et al.   PLANT JOURNAL

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

Precise plant breeding using new genome editing techniques: opportunities, safety and regulation in the EU

(2013) Frank Hartung et al.   PLANT JOURNAL

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants

(2013) Toni Wendt et al.   PLANT MOLECULAR BIOLOGY

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

RNA-Guided Genome Editing for Target Gene Mutations in Wheat

(2013) Santosh Kumar Upadhyay et al.   G3-Genes Genomes Genetics

Transgenic or not? No simple answer!

(2012) Nancy Podevin et al.   EMBO REPORTS

Agbiotech 2.0

(2012) Daniel Gruskin   NATURE BIOTECHNOLOGY

Deployment of new biotechnologies in plant breeding

(2012) Maria Lusser et al.   NATURE BIOTECHNOLOGY

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

Tuber-specific silencing of asparagine synthetase-1 reduces the acrylamide-forming potential of potatoes grown in the field without affecting tuber shape and yield

(2012) Rekha Chawla et al.   PLANT BIOTECHNOLOGY JOURNAL

Advances in plant genome sequencing

(2012) John P. Hamilton et al.   PLANT JOURNAL

Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering

(2012) Y. Zhang et al.   PLANT PHYSIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Renegotiating GM crop regulation

(2011) Jennifer Kuzma et al.   EMBO REPORTS

The development of a cisgenic apple plant

(2011) Thalia Vanblaere et al.   JOURNAL OF BIOTECHNOLOGY

Cisgenic barley with improved phytase activity

(2011) Inger B. Holme et al.   PLANT BIOTECHNOLOGY JOURNAL

Functional analysis and expression profiling of HcrVf1 and HcrVf2 for development of scab resistant cisgenic and intragenic apples

(2011) Sameer G. Joshi et al.   PLANT MOLECULAR BIOLOGY

Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases

(2011) S. J. Curtin et al.   PLANT PHYSIOLOGY

Redefining plant systems biology: from cell to ecosystem

(2011) Joost J.B. Keurentjes et al.   TRENDS IN PLANT SCIENCE

Targeted analysis of nucleotide and copy number variation by exon capture in allotetraploid wheat genome

(2011) Cyrille Saintenac et al.   GENOME BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus

(2010) Katherine M. Han et al.   PLANT BIOTECHNOLOGY JOURNAL

Chromosome engineering: power tools for plant genetics

(2010) Simon W.L. Chan   TRENDS IN BIOTECHNOLOGY

Cisgenesis: an important sub-invention for traditional plant breeding companies

(2009) E. Jacobsen et al.   EUPHYTICA

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A transgenic durum wheat line that is free of marker genes and expresses 1Dy10

(2008) Agata Gadaleta et al.   JOURNAL OF CEREAL SCIENCE

Low-acrylamide French fries and potato chips

(2008) Caius M. Rommens et al.   PLANT BIOTECHNOLOGY JOURNAL