Article
Biochemistry & Molecular Biology
Ruben Quintana-Cabrera, Israel Manjarres-Raza, Carlos Vicente-Gutierrez, Mauro Corrado, Juan P. Bolanos, Luca Scorrano
Summary: The study shows that the mitochondrial fusion and cristae shape protein Opa1 requires mitochondrial ATP synthase oligomers to reduce ROS accumulation. In some cells fueled with galactose to force ATP production by mitochondria, cristae are enlarged, ATP synthase oligomers destabilized, and ROS accumulate.
Article
Chemistry, Multidisciplinary
Dongzhi Xue, Yue Cao, Yinghui Wang, Zhijia Lv, Longhai Jin, Songtao Zhang, Wanying Li, Junduo Wu, Jianhua Liu, Shuyan Song, Xiaogang Liu, Hongjie Zhang
Summary: A novel theranostic nanoagent GLIF based on Gd2(WO4)3:Nd3+ nanoparticles loaded with ploy-L-arginine (PLA), indocyanine green (ICG), and lactoferrin (LF) has been developed for efficient low-dose drug therapy and imaging diagnosis of glioblastoma. GLIF showed significant therapeutic effect and diagnostic performance in vitro and in vivo.
Review
Agronomy
Rupal Singh Tomar, Sunita Kataria, Anjana Jajoo
Summary: This article explores the recent research on signaling and mechanisms for salt tolerance in plants, focusing on the role of the salt overly sensitive system (SOS) and related antiporters proteins and mitogen-activated protein kinase cascades under salt stress. It also highlights the differential expression of various antioxidative enzymes in C3, C4 and CAM plants.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anne Abot, Steven Fried, Patrice D. Cani, Claude Knauf
Summary: The role of reactive oxygen/nitrogen species as friend or foe messengers in the body is well studied, with their effects depending on concentration in tissues. The intestine, affected by oxidation and inflammation, plays a crucial role in diabetic symptoms. Controlling levels of intestinal reactive oxygen/nitrogen species is a complex balance with potential therapeutic implications.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Neurosciences
Olivia J. Kalimon, Hemendra J. Vekaria, Greg A. Gerhardt, Patrick G. Sullivan
Summary: Monoamine oxidase (MAO) is an enzyme that metabolizes amine substrates in the brain, and its inhibitors are used to treat depression and Parkinson's disease. This study investigated the effects of MAO-A and MAO-B inhibitors on brain mitochondrial bioenergetics. The results showed that MAO-A inhibition increased mitochondrial oxygen consumption rate, and higher doses of the inhibitor inhibited bioenergetics and increased reactive oxygen species production. These findings suggest that MAO-A inhibition has a direct effect on brain mitochondrial function, which may be beneficial for patients taking these medications.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Microbiology
Gautier M. Courbon, John L. Rubinstein
Summary: ATP synthases utilize the proton motive force generated by the electron transport chain to synthesize ATP through a remarkable rotary catalytic mechanism. Advances in cryoEM have allowed determination of high-resolution structures and revealed the diversity of ATP synthase structures across species. These studies have important implications for therapeutic targeting of ATP synthases in human diseases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Chui Fann Wong, Wuan-Geok Saw, Sandip Basak, Mio Sano, Hiroshi Ueno, Hwee Wen Kerk, Dennis Litty, Priya Ragunathan, Thomas Dick, Volker Mueller, Hiroyuki Noji, Gerhard Grueber
Summary: The F1FO-ATP synthase is essential for the survival of tuberculosis and nontuberculous mycobacteria and has been confirmed as a potential drug target. Cryo-EM structures of Mycobacterium smegmatis F-1-ATPase and F1FO-ATP synthase with different nucleotide occupancy were determined, revealing critical elements for ATP hydrolysis and synthesis. Mutational studies identified the extended C-terminal domain of subunit alpha as the main element for the self-inhibition mechanism of ATP hydrolysis. Rotation studies showed a rapid transition from the inhibition state to the active state. The mycobacterial-specific elements alpha, gamma, and delta were found to be important for ATP formation, suggesting they could be targeted for species-specific inhibitors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biology
Eiko Nakayama, Toshihiro Kushibiki, Yoshine Mayumi, Ryuichi Azuma, Miya Ishihara, Tomoharu Kiyosawa
Summary: This study investigated the effects of blue laser irradiation on ATP levels in the skin and the generation of reactive oxygen species. The findings highlight the importance of considering the effects on the skin when using blue light for photobiomodulation treatment.
Review
Engineering, Biomedical
Xiaohua Zheng, Yilan Jin, Xiao Liu, Tianqing Liu, Weiqi Wang, Haijun Yu
Summary: Reactive species-based cancer therapies have gained significant attention for their simplicity, controllability, and efficacy. Utilizing nanomaterials for photo-controlled generation of highly reactive radical species is a promising approach for cancer treatment, showing potential synergistic effects with other therapeutic modalities.
BIOACTIVE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Sheikh Mansoor, Owais Ali Wani, Jafar K. Lone, Sweeta Manhas, Navneet Kour, Pravej Alam, Ajaz Ahmad, Parvaiz Ahmad
Summary: Reactive oxygen species (ROS) are highly reactive and dangerous by-products that can cause oxidative cell death, but they also play a beneficial role in regulating biological processes. ROS are generated in various cell compartments of plants and require the action of antioxidants to scavenge them. This review provides a comprehensive overview of the fate of ROS in plants, their role in stress response, and their interaction with other signaling molecules/pathways.
Article
Biochemistry & Molecular Biology
Ana Sanchez-Rodriguez, Ester Sansegundo, Maximiliano Tourmente, Eduardo R. S. Roldan
Summary: Viscosity affects sperm ATP levels and kinematics during capacitation in vitro, with different responses observed in different mouse species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wuan-Geok Saw, Khoa Cong Minh Le, Joon Shin, Jes Hui Min Kwek, Chui Fann Wong, Priya Ragunathan, Tuck Choy Fong, Volker Mueller, Gerhard Grueber
Summary: This study investigates the self-inhibition mechanism of ATP hydrolysis in A. baumannii F-1-ATPase (AbF(1)-ATPase) by generating and purifying a recombinant AbF(1)-ATPase and analyzing its structure. The results reveal that the C-terminal domain of subunit epsilon (Ab epsilon) plays a major role in regulating ATP hydrolysis in AbF(1)-ATPase. The findings have important implications for understanding the regulation of ATP hydrolysis.
Article
Cell Biology
Ester Sansegundo, Maximiliano Tourmente, Eduardo R. S. Roldan
Summary: Mammalian sperm show wide variation in morphology and physiology, especially in terms of ATP levels during capacitation and hyperactivation. The decrease in ATP levels is related to the development of hyperactivated motility and is steeper in species with higher levels of sperm competition. These findings suggest that ATP consumption is higher during capacitation and is associated with higher velocity and head displacement.
Article
Infectious Diseases
Amaravadhi Harikishore, Chui-Fann Wong, Priya Ragunathan, Dennis Litty, Volker Mueller, Gerhard Grueber
Summary: Recent studies have shown that mycobacteria regulate their ATP levels by slowing down respiratory rate and blocking ATP hydrolysis of the F-ATP synthase, with the alpha 533-545 region identified as a major regulator. The alpha 533-545 motif has been found to inhibit rotary coupling with ATP synthesis by docking deep into subunit gamma of the enzyme.
Article
Chemistry, Multidisciplinary
Jia Liu, Lin Shi, Yang Wang, Mingyi Li, Cheng Zhou, Lifang Zhang, Chundong Yao, Ye Yuan, Daan Fu, Yan Deng, Miaodeng Liu, Guobin Wang, Lin Wang, Zheng Wang
Summary: A nanosized ruthenium-based metal-organic framework (Ru-MOF) with high porosity and versatile reactive oxygen and nitrogen species (RONS) scavenging activities has been developed for treating inflammatory diseases.
Article
Biochemistry & Molecular Biology
Alice Biosa, Irene Arduini, Maria Eugenia Soriano, Valentina Giorgio, Paolo Bernardi, Marco Bisaglia, Luigi Bubacco
ACS CHEMICAL NEUROSCIENCE
(2018)
Review
Pharmacology & Pharmacy
Valentina Giorgio, Federico Fogolari, Giovanna Lippe, Paolo Bernardi
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Correction
Biochemistry & Molecular Biology
Alessandra Zanon, Sreehari Kalvakuri, Aleksandar Rakovic, Luisa Foco, Marianna Guida, Christine Schwienbacher, Alice Serafin, Franziska Rudolph, Michaela Trilck, Anne Gruenewald, Nancy Stanslowsky, Florian Wegner, Valentina Giorgio, Alexandros A. Lavdas, Rolf Bodmer, Peter P. Pramstaller, Christine Klein, Andrew A. Hicks, Irene Pichler, Seibler Philip
HUMAN MOLECULAR GENETICS
(2019)
Article
Multidisciplinary Sciences
Andrea Urbani, Valentina Giorgio, Andrea Carrer, Cinzia Franchin, Giorgio Arrigoni, Chimari Jiko, Kazuhiro Abe, Shintaro Maeda, Kyoko Shinzawa-Itoh, Janna F. M. Bogers, Duncan G. G. McMillan, Christoph Gerle, Ildiko Szabo, Paolo Bernardi
NATURE COMMUNICATIONS
(2019)
Editorial Material
Cell Biology
Ulrike B. Hendgen-Cotta, Valentina Giorgio, Livia Hool
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2019)
Article
Cell Biology
Alice Rossi, Giulia Rigotto, Giulia Valente, Valentina Giorgio, Emy Basso, Riccardo Filadi, Paola Pizzo
Review
Biochemistry & Molecular Biology
Chiara Gather, Manuel Jesus Acosta, Giovanni Minervini, Valentina Giorgio
BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
Alice Rossi, Luisa Galla, Chiara Gomiero, Lorena Zentilin, Mauro Giacca, Valentina Giorgio, Tito Cali, Tullio Pozzan, Elisa Greotti, Paola Pizzo
Summary: Alzheimer's disease is a common age-related neurodegenerative disorder caused by mutations in APP, PSEN1, and PSEN2 genes. While PSEN1 familial AD mutations have been associated with a loss-of-function phenotype, further research is needed to determine if this applies to PSEN2 mutations as well.
Article
Cell Biology
Chiara Galber, Giovanni Minervini, Giuseppe Cannino, Francesco Boldrin, Valeria Petronilli, Silvio Tosatto, Giovanna Lippe, Valentina Giorgio
Summary: The f subunit plays a role in ATP synthase dimer stability, and its downregulation leads to abnormal cristae organization and decreased permeability transition pore size. Re-expression of the f subunit in knockdown cells rescues mitochondrial morphology and PTP-dependent swelling.
Review
Biology
Chiara Galber, Stefania Carissimi, Alessandra Baracca, Valentina Giorgio
Summary: Human diseases encompass a wide range of conditions, from gene-associated to gene-non-associated disorders, with mitochondria playing a significant role in the pathogenesis of many diseases, particularly affecting tissues with high-energy metabolism.
Meeting Abstract
Biochemistry & Molecular Biology
Chiara Galber, Simone Fabbian, Cristina Gatto, Martina Grandi, Stefania Carissimi, Manuel Jesus Acosta, Gianluca Sgarbi, Giancarlo Solaini, Alessandra Baracca, Massimo Bellanda, Valentina Giorgio
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Cristina Gatto, Martina Grandi, Cecilia Pucci, Giancarlo Solaini, Alessandra Baracca, Elisabetta Tabolacci, Valentina Giorgio
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Cell Biology
Chiara Galber, Simone Fabbian, Cristina Gatto, Martina Grandi, Stefania Carissimi, Manuel Jesus Acosta, Gianluca Sgarbi, Natascia Tiso, Francesco Argenton, Giancarlo Solaini, Alessandra Baracca, Massimo Bellanda, Valentina Giorgio
Summary: Mitochondrial protein IF1 binds to ATP synthase and inhibits ATP hydrolysis in ischemic tissues. It is overexpressed in tumors and acts as a pro-oncogenic protein. Study found that disruption of ATP5IF1 gene decreases colony formation and tumor mass development in HeLa cells, indicating the role of IF1 in cancer. Lack of IF1 does not affect cell proliferation or mitochondrial respiration, but sensitizes cells to opening of the permeability transition pore (PTP). IF1 binds to ATP synthase OSCP subunit in HeLa cells under oxidative phosphorylation conditions, and this interaction protects cancer cells from PTP-dependent apoptosis under normoxic conditions.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Michela Carraro, Vanessa Checchetto, Geppo Sartori, Roza Kucharczyk, Jean-Paul di Rago, Giovanni Minervini, Cinzia Franchin, Giorgio Arrigoni, Valentina Giorgio, Valeria Petronilli, Silvio C. E. Tosatto, Giovanna Lippe, Ildiko Szabo, Paolo Bernardi
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Manuela Antoniel, Kristen Jones, Salvatore Antonucci, Barbara Spolaore, Federico Fogolari, Valeria Petronilli, Valentina Giorgio, Michela Carraro, Fabio Di Lisa, Michael Forte, Ildiko Szabo, Giovanna Lippe, Paolo Bernardi