Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases

Lymphoid Regeneration from Gene-Corrected SCID-X1 Subject-Derived iPSCs

(2015) Tushar Menon et al.   Cell Stem Cell

Digital PCR to assess hematopoietic chimerism after allogeneic stem cell transplantation

(2015) Tanja Stahl et al.   EXPERIMENTAL HEMATOLOGY

mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5

(2015) U. Mock et al.   NUCLEIC ACIDS RESEARCH

Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA)

(2014) M. Guell et al.   BIOINFORMATICS

Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool

(2014) J. F. Huggett et al.   CLINICAL CHEMISTRY

TALEN-mediated editing of endogenous T-cell receptors facilitates efficient reprogramming of T lymphocytes by lentiviral gene transfer

(2014) B Berdien et al.   GENE THERAPY

OutKnocker: a web tool for rapid and simple genotyping of designer nuclease edited cell lines

(2014) Jonathan L. Schmid-Burgk et al.   GENOME RESEARCH

Targeted genome editing in human repopulating haematopoietic stem cells

(2014) Pietro Genovese et al.   NATURE

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

Isolation of single-base genome-edited human iPS cells without antibiotic selection

(2014) Yuichiro Miyaoka et al.   NATURE METHODS

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

(2014) Holly R. Thomas et al.   PLoS One

Next-Generation Sequencing Platforms

(2013) Elaine R. Mardis   Annual Review of Analytical Chemistry

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

(2013) Kristie Bloom et al.   MOLECULAR THERAPY

Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP

(2013) Andrea Ablasser et al.   NATURE

TALENs and ZFNs are associated with different mutation signatures

(2013) Yongsub Kim et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

FLASH assembly of TALENs for high-throughput genome editing

(2012) Deepak Reyon et al.   NATURE BIOTECHNOLOGY

A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes

(2012) Jonathan L Schmid-Burgk et al.   NATURE BIOTECHNOLOGY

Editing T cell specificity towards leukemia by zinc finger nucleases and lentiviral gene transfer

(2012) Elena Provasi et al.   NATURE MEDICINE

Digital PCR hits its stride

(2012) Monya Baker   NATURE METHODS

A transcription activator-like effector toolbox for genome engineering

(2012) Neville E Sanjana et al.   Nature Protocols

Primer3—new capabilities and interfaces

(2012) Andreas Untergasser et al.   NUCLEIC ACIDS RESEARCH

Site-specific integration and tailoring of cassette design for sustainable gene transfer

(2011) Angelo Lombardo et al.   NATURE METHODS

Cryopreservation and banking of mammalian cell lines

(2009) Glyn N Stacey et al.   Nature Protocols

Quantification of beta-human papillomavirus DNA by real-time PCR

(2009) Sönke J Weissenborn et al.   Nature Protocols

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

(2009) Robert H Kutner et al.   Nature Protocols

Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

(2008) Elena E Perez et al.   NATURE BIOTECHNOLOGY