Review
Biochemistry & Molecular Biology
Si-Wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-Yong Huang, Jia-Bin Cai, Peng-Fei Zhang, Yue-Hong Cui, Ai-Wu Ke
Summary: The CRISPR/Cas9 gene editing technology has made significant progress in oncological research, providing a powerful tool for exploring the mechanism of cancer, establishing tumor models, and studying drug targets.
Review
Biochemistry & Molecular Biology
Chao Zhang, Shanhe Liu, Xuan Li, Ruixuan Zhang, Jun Li
Summary: CRISPR/Cas-based genome editing technologies have revolutionized plant science by allowing precise manipulation of plant genomes. Virus-induced genome editing (VIGE) has emerged as a powerful method for delivering CRISPR/Cas reagents into plant cells, with high editing efficiency and simplified process. This article provides an overview of CRISPR/Cas-based genome editing and focuses on the VIGE systems, types of viruses used for CRISPR/Cas9 cassette delivery, recent applications, and challenges and potential of VIGE in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Materials Science, Biomaterials
Qi Zheng, Weitao Wang, Yuhang Zhou, Jiayin Mo, Xinyue Chang, Zhengbao Zha, Lisha Zha
Summary: Gene editing has great potential in biomedical research, and CRISPR is the most straightforward and cost-effective method. Synthetic nanoparticles have been discovered as effective CRISPR/Cas9 delivery vehicles. This article categorized synthetic nanoparticles for CRISPR/Cas9 delivery, described their advantages and disadvantages, and discussed challenges and potential solutions for clinical applications.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Review
Genetics & Heredity
Mehmet Cengiz Baloglu, Yasemin Celik Altunoglu, Pinar Baloglu, Ali Burak Yildiz, Nil Turkolmez, Yelda Ozden ciftci
Summary: Legumes are rich in protein and phytochemicals, and their genetic traits have been improved using genome editing technologies. However, governmental regulatory restrictions present a concern.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Xinyue Lu, Miaomiao Zhang, Ge Li, Shixin Zhang, Jingbo Zhang, Xiaoge Fu, Fengying Sun
Summary: The rapid advancements in gene therapy have opened up new possibilities for treating genetic disorders. The utilization of CRISPR/Cas9 system enables precise targeting of genes and has demonstrated remarkable efficacy. However, the development of new vectors is necessary to protect nucleic acids from degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Veterinary Sciences
Mariela Lujan Tomazic, Virginia Marugan-Hernandez, Anabel Elisa Rodriguez
Summary: Parasites of the phylum Apicomplexa cause significant diseases and the development of new vaccines has been hampered. The use of omics and systems biology approaches have provided valuable insights and potential targets for vaccine development.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Chemistry, Physical
Zhaoyue Lv, Zhemian Li, Peiran Li, Chi Yao, Dayong Yang
Summary: The CRISPR/Cas system is an efficient gene editing tool, but the delivery of this system for therapeutics remains challenging. DNA nanomaterials, with their excellent molecular recognition capabilities, programmability, and biocompatibility, show promise as delivery carriers for gene agents. This review summarizes recent advances in DNA nanomaterials for CRISPR/Cas system delivery, categorizing them as either branched DNA-based nanostructures or rolling circle amplification (RCA)-based DNA nanostructures. Representative studies on the design of DNA nanomaterials as CRISPR/Cas system delivery carriers are highlighted, and the current challenges and opportunities for their development are discussed. It is anticipated that DNA nanomaterials will provide new possibilities for CRISPR/Cas-based therapeutics.
Review
Biology
Sayed Haidar Abbas Raza, Abdallah A. Hassanin, Sameer D. Pant, Sun Bing, Mahmoud Z. Sitohy, Sameh A. Abdelnour, Mashael Alhumaidi Alotaibi, Tahani Mohamed Al-Hazani, Ayman H. Abd El-Aziz, Gong Cheng, Linsen Zan
Summary: Significant progress has been made in genome editing applications in livestock production using tools such as CRISPR/Cas systems. These tools have the potential to improve animal performance and disease resistance, but also raise ethical and social concerns that must be addressed.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Gargi Bhattacharjee, Nisarg Gohil, Khushal Khambhati, Indra Mani, Rupesh Maurya, Janardhan Keshav Karapurkar, Jigresh Gohil, Dinh-Toi Chu, Hue Vu-Thi, Khalid J. Alzahrani, Pau-Loke Show, Rakesh M. Rawal, Suresh Ramakrishna, Vijai Singh
Summary: A single gene mutation can cause human diseases. The CRISPR-Cas9 system is a powerful tool for correcting these mutations and has been used in various diseases. Effective delivery of CRISPR components is crucial.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Biotechnology & Applied Microbiology
Rupali Chowdhry, Steven Z. Lu, Seungheon Lee, Shivudu Godhulayyagari, Sasha B. Ebrahimi, Devleena Samanta
Summary: Integrating nanotechnology with CRISPR/Cas systems has the potential to overcome limitations, enhance applications in disease diagnostics and therapeutics, and enable faster, more sensitive, and convenient readouts in detection platforms.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Medicine, Research & Experimental
Asma Ghaemi, Elnaz Bagheri, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi
Summary: The prokaryotic CRISPR-Cas systems serve as a revolutionary genome editing tool for modifying, visualizing, and identifying DNA and RNA sequences in live cells of various species. Besides genome editing, the DNA-targeting modifications by CRISPR-Cas offer opportunities for diagnosis, therapy, and genetic disorders investigation. Preclinical studies and CRISPR-Cas9-based therapeutics may encounter challenges, but there is potential for its development in cancer therapy.
Article
Biochemistry & Molecular Biology
Navid Rabiee, Mohammad Rabiee, Soheil Sojdeh, Yousef Fatahi, Rassoul Dinarvand, Moein Safarkhani, Sepideh Ahmadi, Hossein Daneshgar, Fatemeh Radmanesh, Saeid Maghsoudi, Mojtaba Bagherzadeh, Rajender S. Varma, Ebrahim Mostafavi
Summary: In this study, an unpredictable method using high-gravity was introduced to synthesize NH2-MIL-53 for the first time to enhance sensitivity to genetic materials and cellular protein structures. By optimizing the concept phenomenon, interactions between biomolecules and substrates were improved, achieving detection limits of 10 pM for Anti-cas9 protein, 20 pM for ssDNA, less than 10 pM for sgRNA, and around 10 nM for recombinant SARS-CoV-2 spike antigen.
Article
Chemistry, Multidisciplinary
Xiaoyu Xu, Chang Liu, Shengyi Wang, Ermei Makila, Jiali Wang, Oliver Koivisto, Junnian Zhou, Jessica M. Rosenholm, Yilai Shu, Hongbo Zhang
Summary: In this study, a microfluidic-assisted metal-organic framework (MOF) based biomineralization strategy was developed for the efficient and remote control delivery of CRISPR/Cas9 RNP gene editing. The strategy involved growing thermo-responsive EuMOFs onto a photothermal template Prussian blue (PB) and loading RNP during MOFs crystallization in microfluidic channels. The resulting nanocarrier showed high loading efficiency, protection of RNP, and NIR-stimulated release capacity. Upon laser exposure, the nanocarrier demonstrated effective endosomal escape and precise gene knockout. The gene-editing activity could be programmed by tuning exposure times, providing a more controllable editing modality compared to the control group without laser irradiation.
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)