Recent advances in engineering nonribosomal peptide assembly lines

An efficient blue-white screening based gene inactivation system for Streptomyces

(2015) Pengwei Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides

(2015) Hajo Kries et al.   CHEMISTRY & BIOLOGY

Iterative Mechanism of Macrodiolide Formation in the Anticancer Compound Conglobatin

(2015) Yongjun Zhou et al.   CHEMISTRY & BIOLOGY

A stepwise increase in pristinamycin II biosynthesis by Streptomyces pristinaespiralis through combinatorial metabolic engineering

(2015) Lei Li et al.   METABOLIC ENGINEERING

Engineered biosynthesis of enduracidin lipoglycopeptide antibiotics using the ramoplanin mannosyltransferase Ram29

(2015) Ming-Cheng Wu et al.   MICROBIOLOGY-SGM

antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters

(2015) Tilmann Weber et al.   NUCLEIC ACIDS RESEARCH

Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes

(2015) Tilmann Weber et al.   TRENDS IN BIOTECHNOLOGY

Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

(2015) Yongxin Li et al.   Scientific Reports

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Novel fluorinated lipopeptides from Bacillus sp. CS93 via precursor-directed biosynthesis

(2014) Neil K. O’Connor et al.   AMINO ACIDS

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids

(2014) Hajo Kries et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biosynthesis of Novel Pyoverdines by Domain Substitution in a Nonribosomal Peptide Synthetase of Pseudomonas aeruginosa

(2014) Mark J. Calcott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin

(2014) Sophia Zobel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Insights into Secondary Metabolism from a Global Analysis of Prokaryotic Biosynthetic Gene Clusters

(2014) Peter Cimermancic et al.   CELL

Harnessing the Synthetic Capabilities of Glycopeptide Antibiotic Tailoring Enzymes: Characterization of the UK-68,597 Biosynthetic Cluster

(2014) Grace Yim et al.   CHEMBIOCHEM

Oritavancin: First Global Approval

(2014) Anthony Markham   DRUGS

Multiple Complexes of Long Aliphatic N-Acyltransferases Lead to Synthesis of 2,6-Diacylated/2-Acyl-Substituted Glycopeptide Antibiotics, Effectively Killing Vancomycin-Resistant Enterococcus

(2014) Syue-Yi Lyu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange

(2014) Ralf Beer et al.   Molecular BioSystems

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of the linker region joining the adenylation and carrier protein domains of the modular nonribosomal peptide synthetases

(2014) Bradley R. Miller et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes

(2014) Lorena T. Fernández-Martínez et al.   Scientific Reports

Targeted Capture and Heterologous Expression of the Pseudoalteromonas Alterochromide Gene Cluster in Escherichia coli Represents a Promising Natural Product Exploratory Platform

(2014) Avena C. Ross et al.   ACS Synthetic Biology

Rapid and Reliable DNA Assembly via Ligase Cycling Reaction

(2014) Stefan de Kok et al.   ACS Synthetic Biology

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Functional identification of the gene encoding the enzyme involved in mannosylation in ramoplanin biosynthesis in Actinoplanes sp.

(2013) Jun-Sheng Chen et al.   BIOTECHNOLOGY LETTERS

Functional dissection and module swapping of fungal cyclooligomer depsipeptide synthetases

(2013) Dayu Yu et al.   CHEMICAL COMMUNICATIONS

Engineering the Substrate Specificity of the DhbE Adenylation Domain by Yeast Cell Surface Display

(2013) Keya Zhang et al.   CHEMISTRY & BIOLOGY

GenBank

(2013) Dennis A. Benson et al.   NUCLEIC ACIDS RESEARCH

Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

(2013) Sean D. Colloms et al.   NUCLEIC ACIDS RESEARCH

Improved seamless mutagenesis by recombineering using ccdB for counterselection

(2013) Hailong Wang et al.   NUCLEIC ACIDS RESEARCH

Reconstituted Biosynthesis of the Nonribosomal Macrolactone Antibiotic Valinomycin in Escherichia coli

(2013) Jennifer Jaitzig et al.   ACS Synthetic Biology

Engineered Streptomyces avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

(2013) Mamoru Komatsu et al.   ACS Synthetic Biology

Refactoring the Silent Spectinabilin Gene Cluster Using a Plug-and-Play Scaffold

(2013) Zengyi Shao et al.   ACS Synthetic Biology

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

(2012) Jenny Thirlway et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-directed modification of the adenylation domain of the fusaricidin nonribosomal peptide synthetase for enhanced production of fusaricidin analogs

(2012) Jae Woo Han et al.   BIOTECHNOLOGY LETTERS

Glycopeptide Sulfation Evades Resistance

(2012) L. Kalan et al.   JOURNAL OF BACTERIOLOGY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains

(2012) Max Crüsemann et al.   Chemical Science

Inactivation of the Lipopeptide Antibiotic Daptomycin by Hydrolytic Mechanisms

(2011) Vanessa M. D'Costa et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Directed Evolution of a Gatekeeper Domain in Nonribosomal Peptide Synthesis

(2011) Benoit Villiers et al.   CHEMISTRY & BIOLOGY

Directed Evolution of the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives In Vivo

(2011) Bradley S. Evans et al.   CHEMISTRY & BIOLOGY

Nine Enzymes Are Required for Assembly of the Pacidamycin Group of Peptidyl Nucleoside Antibiotics

(2011) Wenjun Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NRPSpredictor2—a web server for predicting NRPS adenylation domain specificity

(2011) Marc Röttig et al.   NUCLEIC ACIDS RESEARCH

Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination

(2011) Lin Zhang et al.   Scientific Reports

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

(2010) Jeffrey H. Kim et al.   BIOPOLYMERS

Active site modification of the β-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of the CDA lipopeptides

(2010) Richard A. Lewis et al.   CHEMICAL COMMUNICATIONS

Enduracidin Analogues with Altered Halogenation Patterns Produced by Genetically Engineered Strains ofStreptomyces fungicidicus

(2010) Xihou Yin et al.   JOURNAL OF NATURAL PRODUCTS

Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs

(2010) Abhijeet Deb Roy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters

(2010) Juan Pablo Gomez-Escribano et al.   Microbial Biotechnology

The Mechanism of Domain Alternation in the Acyl-Adenylate Forming Ligase Superfamily Member 4-Chlorobenzoate: Coenzyme A Ligase,

(2009) Rui Wu et al.   BIOCHEMISTRY

Diversification of echinomycin molecular structure by way of chemoenzymatic synthesis and heterologous expression of the engineered echinomycin biosynthetic pathway

(2009) Kenji Watanabe et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

An Engineered Yeast Efficiently Secreting Penicillin

(2009) Loknath Gidijala et al.   PLoS One

Module Extension of a Non-Ribosomal Peptide Synthetase of the Glycopeptide Antibiotic Balhimycin Produced byAmycolatopsis balhimycina

(2008) Diane Butz et al.   CHEMBIOCHEM

New Pacidamycin Antibiotics Through Precursor-Directed Biosynthesis

(2008) Sabine Grüschow et al.   CHEMBIOCHEM

Non-ribosomal peptide synthetase module fusions to produce derivatives of daptomycin in Streptomyces roseosporus

(2008) S. Doekel et al.   MICROBIOLOGY-SGM

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

(2008) Natalay Kouprina et al.   Nature Protocols

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

(2008) Bagher Amir-Heidari et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Cloning and characterization of new glycopeptide gene clusters found in an environmental DNA megalibrary

(2008) J. J. Banik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA