PINE-TREE enables highly efficient genetic modification of human cell lines

Predicting prime editing efficiency and product purity by deep learning

(2023) Nicolas Mathis et al.   NATURE BIOTECHNOLOGY

Prime editing: advances and therapeutic applications

(2023) Zhihan Zhao et al.   TRENDS IN BIOTECHNOLOGY

Saturation variant interpretation using CRISPR prime editing

(2022) Steven Erwood et al.   NATURE BIOTECHNOLOGY

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

(2022) J. Ferreira da Silva et al.   Nature Communications

Prime Editing Permits the Introduction of Specific Mutations in the Gene Responsible for Duchenne Muscular Dystrophy

(2022) Cédric Happi Mbakam et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Mutation-specific reporter for optimization and enrichment of prime editing

(2022) I. F. Schene et al.   Nature Communications

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

(2022) Dorottya Anna Simon et al.   eLife

Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects

(2022) V. Edwin Hillary et al.   BIOESSAYS

A time-resolved, multi-symbol molecular recorder via sequential genome editing

(2022) Junhong Choi et al.   NATURE

Designing and executing prime editing experiments in mammalian cells

(2022) Jordan L. Doman et al.   Nature Protocols

Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites

(2022) Jianli Tao et al.   Nature Communications

Prime Editing: An All-Rounder for Genome Editing

(2022) Chenyu Lu et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Improving editing efficiency of prime editor in plants

(2022) Niaz Ahmad et al.   TRENDS IN PLANT SCIENCE

Highly efficient generation of isogenic pluripotent stem cell models using prime editing

(2022) Hanqin Li et al.   eLife

Prime Editing Guide RNA Design Automation Using PINE-CONE

(2021) Kylie Standage-Beier et al.   ACS Synthetic Biology

Base editing and prime editing in laboratory animals

(2021) Federico Caso et al.   LABORATORY ANIMALS

APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer’s disease

(2021) Nicholas Brookhouser et al.   MOLECULAR PSYCHIATRY

Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

(2021) Stefan J. Tekel et al.   Nature Protocols

A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing

(2021) Yaojun Tong et al.   Nature Communications

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Comprehensive analysis of prime editing outcomes in human embryonic stem cells

(2021) Omer Habib et al.   NUCLEIC ACIDS RESEARCH

BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment

(2020) Nicholas Brookhouser et al.   Stem Cell Reports

Using human induced pluripotent stem cells (hiPSCs) to investigate the mechanisms by which Apolipoprotein E (APOE) contributes to Alzheimer’s disease (AD) risk

(2020) Sreedevi Raman et al.   NEUROBIOLOGY OF DISEASE

Predicting the efficiency of prime editing guide RNAs in human cells

(2020) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

Prime editing for functional repair in patient-derived disease models

(2020) Imre F. Schene et al.   Nature Communications

A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells

(2020) Nicholas Brookhouser et al.   BMC BIOLOGY

Genome-Scale CRISPR Screens Identify Human Pluripotency-Specific Genes

(2019) Robert J. Ihry et al.   Cell Reports

A transient reporter for editing enrichment (TREE) in human cells

(2019) Kylie Standage-Beier et al.   NUCLEIC ACIDS RESEARCH

Recent advances in the CRISPR genome editing tool set

(2019) Su Bin Moon et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

(2018) Krishna Kumar Haridhasapavalan et al.   GENE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise and efficient scarless genome editing in stem cells using CORRECT

(2017) Dylan Kwart et al.   Nature Protocols

Induced Pluripotent Stem Cells Meet Genome Editing

(2016) Dirk Hockemeyer et al.   Cell Stem Cell

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

(2016) Dominik Paquet et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells

(2015) Chen Yu et al.   Cell Stem Cell

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Playing the End Game: DNA Double-Strand Break Repair Pathway Choice

(2012) J. Ross Chapman et al.   MOLECULAR CELL

Efficient, high-throughput transfection of human embryonic stem cells

(2010) Jennifer C Moore et al.   Stem Cell Research & Therapy