PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

(2021) András Tálas et al.   Nature Communications

GO: a functional reporter system to identify and enrich base editing activity

(2020) Alyna Katti et al.   NUCLEIC ACIDS RESEARCH

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

(2020) Péter István Kulcsár et al.   Nature Communications

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

(2020) Wen Xu et al.   Molecular Plant

BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity

(2020) Peipei Wang et al.   MOLECULAR THERAPY

Directed evolution of adenine base editors with increased activity and therapeutic application

(2020) Nicole M. Gaudelli et al.   NATURE BIOTECHNOLOGY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

(2020) Duran Sürün et al.   Genes

Efficient generation of mouse models with the prime editing system

(2020) Yao Liu et al.   Cell Discovery

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Glycosylase base editors enable C-to-A and C-to-G base changes

(2020) Dongdong Zhao et al.   NATURE BIOTECHNOLOGY

Predicting the efficiency of prime editing guide RNAs in human cells

(2020) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

Unbiased investigation of specificities of prime editing systems in human cells

(2020) Do Yon Kim et al.   NUCLEIC ACIDS RESEARCH

Precise Genome Modification in Tomato Using an Improved Prime Editing System

(2020) Yuming Lu et al.   PLANT BIOTECHNOLOGY JOURNAL

Prime editing for functional repair in patient-derived disease models

(2020) Imre F. Schene et al.   Nature Communications

A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes

(2019) A. St. Martin et al.   Scientific Reports

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

(2019) Tony P. Huang et al.   NATURE BIOTECHNOLOGY

A transient reporter for editing enrichment (TREE) in human cells

(2019) Kylie Standage-Beier et al.   NUCLEIC ACIDS RESEARCH

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

A Universal Surrogate Reporter for Efficient Enrichment of CRISPR/Cas9-Mediated Homology-Directed Repair in Mammalian Cells

(2019) Nana Yan et al.   Molecular Therapy-Nucleic Acids

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

Optimized base editors enable efficient editing in cells, organoids and mice

(2018) Maria Paz Zafra et al.   NATURE BIOTECHNOLOGY

A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells

(2018) Amber St. Martin et al.   NUCLEIC ACIDS RESEARCH

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

(2018) Jason M Gehrke et al.   NATURE BIOTECHNOLOGY

BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B

(2018) Matthew A. Coelho et al.   BMC BIOLOGY

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

(2017) Shengdar Q Tsai et al.   NATURE METHODS

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Open-source guideseq software for analysis of GUIDE-seq data

(2016) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

(2016) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

(2015) Daesik Kim et al.   NATURE METHODS

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations

(2014) Suresh Ramakrishna et al.   Nature Communications

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE