Single-molecule fluorescence resonance energy transfer in molecular biology

Biological oscillations: Fluorescence monitoring by confocal microscopy

(2016) Shyamtanu Chattoraj et al.   CHEMICAL PHYSICS LETTERS

Single-molecule Spectroscopy: Exploring Heterogeneity in Chemical and Biological Systems

(2016) Shirsendu Ghosh et al.   CHEMICAL RECORD

Nanoscale devices for linkerless long-term single-molecule observation

(2016) Niccolò Banterle et al.   CURRENT OPINION IN BIOTECHNOLOGY

Single-Molecule Patch-Clamp FRET Anisotropy Microscopy Studies of NMDA Receptor Ion Channel Activation and Deactivation under Agonist Ligand Binding in Living Cells

(2016) Dibyendu Kumar Sasmal et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale

(2016) Manuel F Juette et al.   NATURE METHODS

Small molecule regulated dynamic structural changes of human G-quadruplexes

(2016) Manish Debnath et al.   Chemical Science

Combining Single-Molecule Imaging and Single-Channel Electrophysiology

(2015) Eve E. Weatherill et al.   JOURNAL OF MOLECULAR BIOLOGY

Probing Single-Molecule Protein Spontaneous Folding–Unfolding Conformational Fluctuation Dynamics: The Multiple-State and Multiple-Pathway Energy Landscape

(2015) Zijian Wang et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

(2015) Iwo König et al.   NATURE METHODS

Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer

(2015) Prasenjit Mondal et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Interrogating the activities of conformational deformed enzyme by single-molecule fluorescence-magnetic tweezers microscopy

(2015) Qing Guo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accumulation of Dynamic Catch Bonds between TCR and Agonist Peptide-MHC Triggers T Cell Signaling

(2014) Baoyu Liu et al.   CELL

High-throughput single-molecule studies of protein-DNA interactions

(2014) Aaron D. Robison et al.   FEBS LETTERS

Application of fluorescence resonance energy transfer in protein studies

(2014) Linlin Ma et al.   JOURNAL OF MOLECULAR STRUCTURE

Single-Molecule Patch-Clamp FRET Microscopy Studies of NMDA Receptor Ion Channel Dynamics in Living Cells: Revealing the Multiple Conformational States Associated with a Channel at Its Electrical Off State

(2014) Dibyendu Kumar Sasmal et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multiplex single-molecule interaction profiling of DNA-barcoded proteins

(2014) Liangcai Gu et al.   NATURE

Continuous throughput and long-term observation of single-molecule FRET without immobilization

(2014) Swati Tyagi et al.   NATURE METHODS

Single-molecule motions enable direct visualization of biomolecular interactions in solution

(2014) Quan Wang et al.   NATURE METHODS

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

(2014) Bi-Chang Chen et al.   SCIENCE

Sizing up single-molecule enzymatic conformational dynamics

(2013) H. Peter Lu   CHEMICAL SOCIETY REVIEWS

Single-molecule spectroscopy of protein folding dynamics—expanding scope and timescales

(2013) Benjamin Schuler et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

An Optical Trap Combined with Three-Color FRET

(2013) Sanghwa Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A single-molecule dissection of ligand binding to a protein with intrinsic dynamics

(2013) Eunkyung Kim et al.   Nature Chemical Biology

Single-molecule DNA digestion in various alkanethiol-functionalized gold nanopores

(2013) Seungah Lee et al.   TALANTA

Simple super-resolution live-cell imaging based on diffusion-assisted Förster resonance energy transfer

(2013) Sangyeon Cho et al.   Scientific Reports

Single-molecule FRET of protein structure and dynamics - a primer

(2013) Benjamin Schuler   JOURNAL OF NANOBIOTECHNOLOGY

Manipulating Protein Conformations by Single-Molecule AFM-FRET Nanoscopy

(2012) Yufan He et al.   ACS Nano

Measurement of the Activity of Individual Subunits of Single Molecules of the Tetrameric Enzyme β-Galactosidase

(2012) Douglas B. Craig et al.   ANALYTICAL CHEMISTRY

Optical Methods for Single Molecule Detection and Analysis

(2012) David R. Walt   ANALYTICAL CHEMISTRY

Determination of Interaction Kinetics between the T Cell Receptor and Peptide-Loaded MHC Class II via Single-Molecule Diffusion Measurements

(2012) Markus Axmann et al.   BIOPHYSICAL JOURNAL

Single molecule enzyme-linked immunosorbent assays: Theoretical considerations

(2012) Lei Chang et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

(2012) Sami Hocine et al.   NATURE METHODS

A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins

(2012) Jiajie Diao et al.   Nature Protocols

Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

(2012) Minjoung Kyoung et al.   Nature Protocols

Single-Molecule Fluorescence Experiments Determine Protein Folding Transition Path Times

(2012) H. S. Chung et al.   SCIENCE

Single-Molecule Lysozyme Dynamics Monitored by an Electronic Circuit

(2012) Y. Choi et al.   SCIENCE

Protein folding at single-molecule resolution

(2011) Allan Chris M. Ferreon et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Single Vesicle Assaying of SNARE-Synaptotagmin-Driven Fusion Reveals Fast and Slow Modes of Both Docking and Fusion and Intrasample Heterogeneity

(2011) Sune M. Christensen et al.   BIOPHYSICAL JOURNAL

Conformational Plasticity and Dynamics in the Generic Protein Folding Catalyst SlyD Unraveled by Single-Molecule FRET

(2011) Dana Kahra et al.   JOURNAL OF MOLECULAR BIOLOGY

Probing Single-Molecule Enzyme Active-Site Conformational State Intermittent Coherence

(2011) Yufan He et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Central dogma at the single-molecule level in living cells

(2011) Gene-Wei Li et al.   NATURE

Probing cellular protein complexes using single-molecule pull-down

(2011) Ankur Jain et al.   NATURE

Structural landscape of isolated agonist-binding domains from single AMPA receptors

(2011) Christy F Landes et al.   Nature Chemical Biology

Optical tweezers study life under tension

(2011) Furqan M Fazal et al.   Nature Photonics

Convex Lens-Induced Confinement for Imaging Single Molecules

(2010) Sabrina R. Leslie et al.   ANALYTICAL CHEMISTRY

Single SNARE-Mediated Vesicle Fusion Observed In Vitro by Polarized TIRFM

(2010) Volker Kiessling et al.   BIOPHYSICAL JOURNAL

Single-molecule dynamics of gating in a neurotransmitter transporter homologue

(2010) Yongfang Zhao et al.   NATURE

TCR–peptide–MHC interactions in situ show accelerated kinetics and increased affinity

(2010) Johannes B. Huppa et al.   NATURE

Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations

(2010) David M Rissin et al.   NATURE BIOTECHNOLOGY

Monitoring multiple distances within a single molecule using switchable FRET

(2010) Stephan Uphoff et al.   NATURE METHODS

Single-Molecule Three-Color FRET with Both Negligible Spectral Overlap and Long Observation Time

(2010) Sanghwa Lee et al.   PLoS One

A fast, single-vesicle fusion assay mimics physiological SNARE requirements

(2010) Erdem Karatekin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic Ca2+-Dependent Stimulation of Vesicle Fusion by Membrane-Anchored Synaptotagmin 1

(2010) H. K. Lee et al.   SCIENCE

A single-vesicle content mixing assay for SNARE-mediated membrane fusion

(2010) Jiajie Diao et al.   Nature Communications

Ultrasensitive Electrochemical DNA Assay Based on Counting of Single Magnetic Nanobeads by a Combination of DNA Amplification and Enzyme Amplification

(2009) Xiaoli Zhang et al.   ANALYTICAL CHEMISTRY

Single-Molecule Studies of the Neuronal SNARE Fusion Machinery

(2009) Axel T. Brunger et al.   Annual Review of Biochemistry

Single-Molecule Approaches to Stochastic Gene Expression

(2009) Arjun Raj et al.   Annual Review of Biophysics

Lipid Mixing and Content Release in Single-Vesicle, SNARE-Driven Fusion Assay with 1–5 ms Resolution

(2009) Tingting Wang et al.   BIOPHYSICAL JOURNAL

Mechanistic Aspects of Horseradish Peroxidase Elucidated through Single-Molecule Studies

(2009) Hans H. Gorris et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RNA folding dynamics by single-molecule fluorescence resonance energy transfer

(2009) Rui Zhao et al.   METHODS

A high-resolution magnetic tweezer for single-molecule measurements

(2009) Kipom Kim et al.   NUCLEIC ACIDS RESEARCH

Biology, one molecule at a time

(2009) Achillefs N. Kapanidis et al.   TRENDS IN BIOCHEMICAL SCIENCES

A single molecule view of gene expression

(2009) Daniel R. Larson et al.   TRENDS IN CELL BIOLOGY

Single-Molecule Studies of Protein Folding

(2008) Alessandro Borgia et al.   Annual Review of Biochemistry

Protein folding studied by single-molecule FRET

(2008) Benjamin Schuler et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Unfolded Protein and Peptide Dynamics Investigated with Single-Molecule FRET and Correlation Spectroscopy from Picoseconds to Seconds†

(2008) Daniel Nettels et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Fluorescence resonance energy transfer and anisotropy reveals both hetero- and homo-energy transfer in the pleckstrin homology-domain and the parathyroid hormone-receptor

(2008) Ralf Steinmeyer et al.   MICROSCOPY RESEARCH AND TECHNIQUE

A practical guide to single-molecule FRET

(2008) Rahul Roy et al.   NATURE METHODS

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

(2008) Keir C Neuman et al.   NATURE METHODS

Membrane fusion

(2008) William Wickner et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quantum dot-based resonance energy transfer and its growing application in biology

(2008) Igor L. Medintz et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Multiscale complex network of protein conformational fluctuations in single-molecule time series

(2008) C.-B. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Molecule DNA Sequencing of a Viral Genome

(2008) T. D. Harris et al.   SCIENCE