期刊
NATURE METHODS
卷 11, 期 3, 页码 297-U358出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2809
关键词
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资金
- Emmy-Noether program of the Deutsche Forschungsgemeinschaft (DFG)
- Darwin Trust of Edinburgh
- Boehringer Ingelheim Fonds
- EMBL interdisciplinary postdoctoral program (EIPOD)
- Programa VALi+d of the Generalitat Valenciana fellowships
We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.
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