Approaches for high-throughput quantification of periplasmic recombinant proteins

Signal Peptide Efficiency: From High-Throughput Data to Prediction and Explanation

(2023) Stefano Grasso et al.   ACS Synthetic Biology

Biopharmaceutical benchmarks 2022

(2022) Gary Walsh et al.   NATURE BIOTECHNOLOGY

The Dynamic SecYEG Translocon

(2021) Julia Oswald et al.   Frontiers in Molecular Biosciences

Engineering a Supersecreting Strain of Escherichia coli by Directed Coevolution of the Multiprotein Tat Translocation Machinery

(2021) May N. Taw et al.   ACS Synthetic Biology

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

(2021) Alexandros Karyolaimos et al.   Frontiers in Bioengineering and Biotechnology

High-Throughput Screening Technology in Industrial Biotechnology

(2020) Weizhu Zeng et al.   TRENDS IN BIOTECHNOLOGY

Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

(2020) Kiavash Mirzadeh et al.   Microbial Cell Factories

Superfolder mTurquoise2ox optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets

(2019) Nils Y. Meiresonne et al.   MOLECULAR MICROBIOLOGY

Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

(2019) Alexandros Karyolaimos et al.   Frontiers in Microbiology

Transport of Folded Proteins by the Tat System

(2019) Kelly M. Frain et al.   PROTEIN JOURNAL

Microdroplet screening and selection for improved microbial production of extracellular compounds

(2019) Rinke J van Tatenhove-Pel et al.   CURRENT OPINION IN BIOTECHNOLOGY

Signal peptides for recombinant protein secretion in bacterial expression systems

(2018) Roland Freudl   Microbial Cell Factories

Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli

(2018) Tania Selas Castiñeiras et al.   Scientific Reports

Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis

(2018) Gang Fu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Tuning recombinant protein expression to match secretion capacity

(2018) Luminita Gabriela Horga et al.   Microbial Cell Factories

Tailoring Escherichia coli for the l-Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins

(2017) Anna Hjelm et al.   ACS Synthetic Biology

Escherichia coli surface display for the selection of nanobodies

(2017) Valencio Salema et al.   Microbial Biotechnology

Enhanced secretion of recombinant proteins via signal recognition particle (SRP)-dependent secretion pathway by deletion of rrsE in Escherichia coli

(2016) Yong Jae Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag

(2016) Na Ke et al.   JOURNAL OF BACTERIOLOGY

SecA Cotranslationally Interacts with Nascent Substrate Proteins In Vivo

(2016) Damon Huber et al.   JOURNAL OF BACTERIOLOGY

Global profiling of SRP interaction with nascent polypeptides

(2016) Daniela Schibich et al.   NATURE

Crystal structure of a substrate-engaged SecY protein-translocation channel

(2016) Long Li et al.   NATURE

Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli

(2016) Dmitri Dormeshkin et al.   PROTEIN EXPRESSION AND PURIFICATION

A palette of fluorescent proteins optimized for diverse cellular environments

(2015) Lindsey M. Costantini et al.   Nature Communications

Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system

(2015) T. Noelle Lombana et al.   Scientific Reports

Proteome-wide Subcellular Topologies ofE. coliPolypeptides Database (STEPdb)

(2014) Georgia Orfanoudaki et al.   MOLECULAR & CELLULAR PROTEOMICS

Optimisation of signal peptide for recombinant protein secretion in bacterial hosts

(2013) Kheng Oon Low et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Co-translational protein targeting to the bacterial membrane

(2013) Ishu Saraogi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Structure and mechanism of Escherichia coli type I signal peptidase

(2013) Mark Paetzel   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Recombinant protein production in bacterial hosts

(2013) Tim W. Overton   DRUG DISCOVERY TODAY

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Universal Genetic Assay for Engineering Extracellular Protein Expression

(2013) Charles H. Haitjema et al.   ACS Synthetic Biology

Fusion protein linkers: Property, design and functionality

(2012) Xiaoying Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Combinatorial Mutagenesis and Selection of Improved Signal Sequences and Their Application for High-Level Production of Translocated Heterologous Proteins in Escherichia coli

(2012) Tonje Marita Bjerkan Heggeset et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The twin-arginine translocation (Tat) protein export pathway

(2012) Tracy Palmer et al.   NATURE REVIEWS MICROBIOLOGY

Development of Cysteine-Free Fluorescent Proteins for the Oxidative Environment

(2012) Takahisa Suzuki et al.   PLoS One

Characterization of the Escherichia coli SecA Signal Peptide-Binding Site

(2011) L. M. Grady et al.   JOURNAL OF BACTERIOLOGY

Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies

(2011) T. Dinh et al.   JOURNAL OF BACTERIOLOGY

SecA Interacts with Ribosomes in Order to Facilitate Posttranslational Translocation in Bacteria

(2011) Damon Huber et al.   MOLECULAR CELL

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Production of disulfide-bonded proteins in Escherichia coli

(2011) Mehmet Berkmen   PROTEIN EXPRESSION AND PURIFICATION

Superfolder GFP Is Fluorescent in Oxidizing Environments When Targeted via the Sec Translocon

(2011) Deborah E. Aronson et al.   TRAFFIC

Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

(2010) C. Degering et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide

(2010) Michael Caspers et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Antibody fragments

(2010) Aaron L. Nelson   mAbs

Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies

(2010) Tomohiro Makino et al.   METABOLIC ENGINEERING

A rapid protein folding assay for the bacterial periplasm

(2010) Thomas J. Mansell et al.   PROTEIN SCIENCE

The fluorescent protein palette: tools for cellular imaging

(2009) Richard N. Day et al.   CHEMICAL SOCIETY REVIEWS

Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high-quality recombinant proteins

(2009) Yanina Sevastsyanovich et al.   FEMS MICROBIOLOGY LETTERS

Biased codon usage in signal peptides: a role in protein export

(2009) Yaramah M. Zalucki et al.   TRENDS IN MICROBIOLOGY

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Laboratory Evolution of Fast-Folding Green Fluorescent Protein Using Secretory Pathway Quality Control

(2008) Adam C. Fisher et al.   PLoS One

Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging

(2008) M. C. Leake et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA