Article
Cell Biology
Hongchen Yan, Jun-Jie Xu, Ilyas Ali, Wei Zhang, Ming Jiang, Guiping Li, Yong Teng, Guangxun Zhu, Yafei Cai
Summary: CDK5RAP3 plays a crucial role in cell growth and interacts with RPL26 to regulate the mTOR pathway. Its deficiency can lead to instability in cell growth and cell cycle regulation.
CELL PROLIFERATION
(2022)
Article
Pharmacology & Pharmacy
Peng Cheng, Jiawei Wu, Gangfan Zong, Feihui Wang, Rui Deng, Ruizhi Tao, Cheng Qian, Yunlong Shan, Aiyun Wang, Yang Zhao, Zhonghong Wei, Yin Lu
Summary: Dietary factors play a vital role in tumorigenesis. The effect of a spicy diet on cancer development, particularly colon cancer metastasis, has been controversial. In this study, a mouse metastasis model was used to investigate the role of capsaicin in metastasis. The long-term administration of capsaicin diet (300 mg/kg) in mice promoted the formation of a liver pre-metastatic niche, facilitating the metastasis of colon cancer cells. Bacteria translocation to the liver was observed, and capsaicin altered the composition of gut microbiota, increasing intestinal barrier permeability and disrupting the gut vascular barrier. The dysregulated bile acids profile affected the recruitment of natural killer T (NKT) cells in the pre-metastatic niche, with primary bile acid alpha-Muricholic acid enhancing NKT cell recruitment and secondary bile acids Glycoursodeoxycholic acid and Taur-ohyodeoxycholic acid impairing NKT cell recruitment. These findings highlight the increased risk of cancer metastasis through gut microbiota modulation caused by long-term consumption of capsaicin.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Biology
William J. Allen, Robin A. Corey, Daniel W. Watkins, A. Sofia F. Oliveira, Kiel Hards, Gregory M. Cook, Ian Collinson
Summary: Transport of proteins across and into membranes is a fundamental process in biology. Recent studies have shown that the diffusion of arginine residues limits pre-protein transport, while lysine can be transported across membranes in its neutral form. These findings have implications for understanding the mechanism of protein secretion and suggest a potential role of proton-motive force in aiding transport.
Article
Biochemistry & Molecular Biology
Guillaume Roussel, Eric Lindner, Stephen H. White
Summary: By measuring the fluorescence intensity and wavelength shift change of the polarity-sensitive fluorophore NBD, we determined the disposition of SecA on the Escherichia coli membrane. Our results showed that SecA is primarily anchored in the membrane interface by the positive charges of the N terminus domain, and a region of the nucleotide binding domain II is also important for binding. The topology of SecA on the membrane is consistent with previous structural observations.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jin Xu, Sidi Liu, Licong Cai, Lingyu Wang, Yufei Dong, Zhenyu Qi, Jingquan Yu, Yanhong Zhou
Summary: The post-translational modification of proteins enables cells to respond promptly to dynamic stimuli by controlling protein functions. In this study, researchers found that SlSPY plays a positive regulatory role in fruit ripening in tomatoes by changing the ethylene response. They also discovered that SlSPY interacts with a central regulator of ethylene signaling, SlEIN2, promoting its stability and nuclear accumulation. These findings reveal a novel regulatory function of SlSPY in fruit ripening and provide new insights into the role of the SlSPY-SlEIN2 module in tomato fruit ripening.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou
Summary: This study reveals the role of ECD in PepT1, confirming its importance in protein function, and providing new insight into the transport mechanism of PepT1.
Article
Cell Biology
Srinath Krishnamurthy, Marios-Frantzeskos Sardis, Nikolaos Eleftheriadis, Katerina E. Chatzi, Jochem H. Smit, Konstantina Karathanou, Giorgos Gouridis, Athina G. Portaliou, Ana-Nicoleta Bondar, Spyridoula Karamanou, Anastassios Economou
Summary: Protein machines undergo conformational motions to interact with and manipulate polymeric substrates. The intrinsic dynamics of Sec translocase ATPase, SecA, and the binding of preproteins combine to achieve translocation. This universal mechanism allows any preprotein to undergo translocation and be secreted.
Article
Biochemistry & Molecular Biology
Weiwei Zhang, Lei Huang, Chunhua Zhang, Christopher J. Staiger
Summary: The study demonstrates the involvement of Arabidopsis myosin XIK in the tethering step of exocytosis through direct interactions with the exocyst complex. XIK associates with secretory vesicles earlier than exocyst and plays a role in efficient localization of exocyst complex at the plasma membrane tethering site.
Article
Oncology
Shigeo Ohba, Yongjian Tang, Tor-Christian Aase Johannessen, Joydeep Mukherjee
Summary: PKM2 interacts with and enhances the activation of the Cdk1-cyclin B complex, thereby directly controlling the progression of mitosis and the growth of brain tumor cells.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Xinfang Yu, Wei Li, Ken H. Young, Yong Li
Summary: PD-L1 is a classic immune checkpoint molecule with its expression regulated by posttranslational modifications (PTMs) that play vital roles in controlling PD-L1 expression, cellular trafficking, and antitumor immune response.
Article
Multidisciplinary Sciences
Xinchen Du, Le Wu, Hongyu Yan, Zhuyan Jiang, Shilin Li, Wen Li, Yanli Bai, Hongjun Wang, Zhaojun Cheng, Deling Kong, Lianyong Wang, Meifeng Zhu
Summary: Researchers have developed an alkylated chitosan sponge with highly interconnective microchannels, demonstrating its advantages in anti-infective, pro-coagulant, hemostatic, and wound healing capacities, making it suitable for treating noncompressible hemorrhages.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Chen Bao, Mingyi Zhu, Inna Nykonchuk, Hironao Wakabayashi, David H. Mathews, Dmitri N. Ermolenko
Summary: This study demonstrates that specific length and structure, rather than high thermodynamic stability, are crucial for regulatory mRNA stem-loops to pause translation. These findings provide insights into the identification of new regulatory mRNA stem-loops.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Hong-Yi Liu, Ying-Ying Liu, Yin-Ling Zhang, Yan Ning, Fang-Lin Zhang, Da-Qiang Li
Summary: The study found that NAT10 undergoes covalent PARylation modification following DNA damage, with PARP1 catalyzing PARylation on three conserved lysine residues within its C-terminal nucleolar localization signal. These findings reveal the key role of PARP1-mediated PARylation of NAT10 in controlling its nucleoplasmic translocation and function in response to DNA damage.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Virology
Weijie Chen, Yukun Li, Xiuling Yu, Zhenwei Wang, Wenbiao Wang, Menglan Rao, Yongkui Li, Zhen Luo, Qiwei Zhang, Jinbiao Liu, Jianguo Wu
Summary: In this study, it was found that Zika virus infection upregulated 7-dehydrocholesterol reductase (DHCR7) expression, which in turn facilitated viral infection. The nonstructural protein NS4B of Zika virus interacted with DHCR7 to induce its expression, thereby inhibiting the immune response and facilitating viral evasion. This study expands our understanding of the mechanisms by which viral nonstructural proteins antagonize innate immunity during viral infection via cholesterol metabolic enzymes and intermediates.
Article
Multidisciplinary Sciences
Kristina Krasich, Claire Simmons, Kevin O'Neill, Charles M. Giattino, Felipe De Brigard, Walter Sinnott-Armstrong, Liad Mudrik, Marty G. Woldorff
Summary: The study found that prestimulus alpha activity plays an important role in conscious perception. Attenuated prestimulus alpha power is associated with stimulus-evoked recurrent processing, but it does not necessarily result in better perception. On the other hand, elevated prestimulus alpha power at a specific phase is associated with better cue perception.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Tamar Cranford-Smith, Mohammed Jamshad, Mark Jeeves, Rachael A. Chandler, Jack Yule, Ashley Robinson, Farhana Alam, Karl A. Dunne, Edwin H. Aponte Angarita, Mashael Alanazi, Cailean Carter, Ian R. Henderson, Janet E. Lovett, Peter Winn, Timothy Knowles, Damon Huber
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Matthew L. Kraushar, Ferdinand Krupp, Dermot Harnett, Paul Turko, Mateusz C. Ambrozkiewicz, Thiemo Sprink, Koshi Imami, Manuel Guennigmann, Ulrike Zinnall, Carlos H. Vieira-Vieira, Theres Schaub, Agnieszka Muenster-Wandowski, Joerg Buerger, Ekaterina Borisova, Hiroshi Yamamoto, Mladen-Roko Rasin, Uwe Ohler, Dieter Beule, Thorsten Mielke, Victor Tarabykin, Markus Landthaler, Guenter Kramer, Imre Vida, Matthias Selbach, Christian M. T. Spahn
Summary: This study explores the architecture of ribosomes in the developing nervous system and identifies Ebp1 as a crucial factor in protein synthesis, playing a key role in cell morphology and the synthesis of membrane-targeted cell adhesion molecules. Ebp1 regulates ribosome occupancy of specific codons during different stages of protein synthesis in the neocortex, highlighting its importance in neuronal development.
Correction
Multidisciplinary Sciences
Anne S. Wentink, Nadinath B. Nillegoda, Jennifer Feufel, Gabriele Ubartaite, Carolyn P. Schneider, Paolo de los Rios, Janosch Hennig, Alessandro Barducci, Bernd Bukau
Summary: A correction to this paper has been published at https://doi.org/10.1038/s41586-020-03090-x.
Article
Multidisciplinary Sciences
Matilde Bertolini, Kai Fenzl, Ilia Kats, Florian Wruck, Frank Tippmann, Jaro Schmitt, Josef Johannes Auburger, Sander Tans, Bernd Bukau, Gunter Kramer
Summary: This study investigated whether newly synthesized proteins can form functional oligomers through co-co assembly. They found that this mechanism can lead to the co-assembly of hundreds of homomeric subunits in human cells, mainly mediated by N-terminal coiled coils among five major domain classes.
Article
Biology
Steffen Preissler, Claudia Rato, Yahui Yan, Luke A. Perera, Aron Czako, David Ron
Article
Cell Biology
Ulrike Anne Friedrich, Mostafa Zedan, Bernd Hessling, Kai Fenzl, Ludovic Gillet, Joseph Barry, Michael Knop, Guenter Kramer, Bernd Bukau
Summary: N-terminal acetylation is a common protein modification in eukaryotes, catalyzed by multiple N-terminal acetyltransferases (Nats). This study reveals that NatA is involved in systemic adaptation control, while NatB predominantly affects protein folding. These findings suggest that controlling Nat activities may lead to different cellular responses.
Article
Multidisciplinary Sciences
Luke A. Perera, Steffen Preissler, Nathan R. Zaccai, Sylvain Prevost, Juliette M. Devos, Michael Haertlein, David Ron
Summary: This study reveals the mechanism of regulation of the endoplasmic reticulum chaperone BiP by FICD-mediated AMPylation and deAMPylation, characterizing the structure of AMPylated BiP bound to FICD through X-ray crystallography and neutron scattering, shedding light on the process of BiP AMPylation and deAMPylation.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Jiri Koubek, Jaro Schmitt, Carla Veronica Galmozzi, Guenter Kramer
Summary: Growing cells dedicate a significant part of their biosynthetic capacity to producing proteins, which must undergo various processes for proper functionality. Integration of translation with protein maturation processes is crucial for efficient and accurate protein synthesis. The interplay of ribosomes, chaperones, and other factors during cotranslational protein maturation guides the sequential events and coupling with protein complex assembly.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Ajeet K. Sharma, Johannes Venezian, Ayala Shiber, Guenter Kramer, Bernd Bukau, Edward P. O'Brien
Summary: A single cluster of nonoptimal codons in Saccharomyces cerevisiae can reduce a transcript's half-life, while the impact of multiple clusters remains unknown. A kinetic model predicts that inserting a second nonoptimal cluster near the 5' end can increase mRNA half-life, which was experimentally validated. The results suggest that codon usage bias has a more nuanced role in controlling cellular protein levels than previously thought.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Federico Cerullo, Sebastian Filbeck, Pratik Rajendra Patil, Hao-Chih Hung, Haifei Xu, Julia Vornberger, Florian W. Hofer, Jaro Schmitt, Guenter Kramer, Bernd Bukau, Kay Hofmann, Stefan Pfeffer, Claudio A. P. Joazeiro
Summary: This study uncovers the function of Bacillus subtilis MutS2, a member of the conserved MutS family, in translational quality control. MutS2 acts as a ribosome-binding protein and functions in sensing collisions between stalled and translating ribosomes. It promotes ribosomal subunit dissociation, ribosome recycling, and initiation of ribosome-associated protein quality control. These findings demonstrate the conserved role of ribosome collisions in mounting a complex response to translation interruption.
Article
Multidisciplinary Sciences
Mohsin M. Naqvi, Mario J. Avellaneda, Andrew Roth, Eline J. Koers, Antoine Roland, Vanda Sunderlikova, Gunter Kramer, Hays S. Rye, Sander J. Tans
Summary: In cells, the chaperonin GroEL-ES can accelerate protein folding by strengthening the collapse of polypeptide chains. This collapse enhancement is strongest when GroEL is in its nucleotide-bound states and is aided by GroES binding and the presence of amphiphilic C-terminal tails. This finding highlights the importance of collapse modulation in protein quality control.
Article
Biochemistry & Molecular Biology
Joseph George Beton, Jim Monistrol, Anne Wentink, Erin C. Johnston, Anthony John Roberts, Bernd Gerhard Bukau, Bart W. Hoogenboom, Helen R. Saibil
Summary: Specific combinations of molecular chaperones in the human body can disassemble protein aggregates, which is important for combating disease-related fiber formation. This study reveals the mechanism of this disassembly process and the localization and activity of molecular chaperones on the fibers using techniques such as atomic force microscopy.