期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 60, 期 9, 页码 2065-2075出版社
WILEY-BLACKWELL
DOI: 10.1002/mnfr.201500892
关键词
Adipogenesis; Brite adipocyte; Human adipocytes; Mitochondrial function; Polyunsaturated fatty acids
资金
- Ministerium fur Wissenschaft und Forschung des Landes Nordrhein-Westfalen (Ministry of Science and Research of the State of North Rhine-Westphalia)
- Bundesministerium fur Gesundheit (Federal Ministry of Health)
- Training and Feasibility Grant of the German Diabetes Center
- FP7 Marie Curie Intra-European fellowship [ADDIO-PIEF-2012-328793]
Scope: n-3 and n-6 PUFAs have several opposing biological effects and influence white adipose tissue (WAT) function. The recent discovery of thermogenic UCP1-expressing brite adipocytes within WAT raised the question whether n-3 and n-6 PUFAs exert differential effects on brite adipocyte formation and mitochondrial function. Methods and results: Primary human preadipocytes were treated with n-3 PUFAs (eicosapentaenoic acid, EPA; docosahexaenoic acid, DHA) or n-6 PUFA (arachidonic acid, ARA) during differentiation, and adipogenesis, white and brite gene expression markers, mitochondrial content and function were analyzed at day 12 of differentiation. Adipogenesis was equally increased by n-3 and n-6 PUFAs. The n-6 PUFA ARA increased lipid droplet size and expression of the white-specific marker TCF21 while decreased mitochondrial protein expression and respiratory function. In contrast, EPA increased expression of the brown adipocyte-related genes UCP1 and CPT1B, and improved mitochondrial function of adipocytes. The opposing effects of EPA and ARA on gene expression and mitochondrial function were also observed in cells treated from day 8 to 12 of adipocyte differentiation. Conclusion: EPA promotes brite adipogenesis and improves parameters of mitochondrial function, such as increased expression of CPTB1, citrate synthase activity and higher maximal respiratory capacity, while ARA reduced mitochondrial spare respiratory capacity in vitro.
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