Review
Plant Sciences
Styliani N. Chorianopoulou, Dimitris L. Bouranis
Summary: Sulfur is an essential macronutrient for plant growth and metabolism, and it plays diverse roles in their life cycles. It is connected to the homeostasis of essential micronutrients, such as iron, copper, zinc, and manganese. Sulfur is involved in the uptake, transportation, and distribution of these micronutrients, and it interacts with them in functional ways.
Article
Biology
Gilberto Garcia, Hanlin Zhang, Sophia Moreno, C. Kimberly Tsui, Brant Michael Webster, Ryo Higuchi-Sanabria, Andrew Dillin, Randy Y. Hampton
Summary: Changes in lipid metabolism are associated with aging and age-related diseases, including proteopathies. This study identified let-767 as an essential gene for both lipid and ER protein homeostasis in Caenorhabditis elegans. Knockdown of let-767 affects lipid stores, ER morphology, and the induction of the UPRER. A reduction in lipogenic pathways restores UPRER induction in animals with reduced let-767 by blocking the metabolite 3-oxoacyl.
Article
Microbiology
Tsung-Yu Chao, Yi-Ying Cheng, Zi-Yun Wang, Tien-Fang Fang, Yu-Ruei Chang, Chi-Shane Fuh, Mei-Tzu Su, Yuan-Wei Su, Pang-Hung Hsu, Yu-Chen Su, Yu-Ching Chang, Ting-Yau Lee, Wei-Han Chou, Jaap M. Middeldorp, Jaakko Saraste, Mei-Ru Chen
Summary: Upon EBV lytic reactivation, the virus-encoded DNA replication machinery functions in the nucleus, while the newly synthesized DNA is encapsidated and transported to the cytoplasm for final virus assembly. The single-stranded DNA binding protein BALF2 executing functions within the nucleus was also identified in the tegument layer of mature virions. We are interested in how BALF2 is packaged into the tegument of virions.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biochemistry & Molecular Biology
Grace Kennedy, Olivia Gibson, Daire T. O'Hare, Ian G. Mills, Emma Evergren
Summary: Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a serine/threonine protein kinase involved in regulating energy homeostasis and cell growth. Its deregulation has implications in metabolic diseases and cancer. Recent research has explored new functions of CaMKK2, such as lipogenesis and Golgi vesicle trafficking. This review discusses the role of CaMKK2 in membrane trafficking mechanisms and its implications for disease.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Yulong Ren, Yongfei Wang, Yu Zhang, Tian Pan, Erchao Duan, Xiuhao Bao, Jianping Zhu, Xuan Teng, Pengcheng Zhang, Chuanwei Gu, Hui Dong, Fan Wang, Yunlong Wang, Yiqun Bao, Yihua Wang, Jianmin Wan
Summary: Seed storage proteins (SSPs) are the major protein nutrition sources for human and livestock, and they are transported through a plant-specific endomembrane system. Recent studies have uncovered the complex network controlling SSP trafficking, involving both evolutionarily conserved molecular machineries and plant-unique regulators. This review summarizes the current knowledge of protein body biogenesis and endomembrane-mediated SSP transport, and discusses cutting-edge strategies for advancing the field.
Review
Biochemistry & Molecular Biology
Miaomiao Li, Rongrong Liu, Yaping Zhao, Pengfei Liu
Summary: GS28 is a transmembrane protein that localizes to the Golgi apparatus and plays a crucial role in regulating vesicle trafficking. It is involved in various intracellular transport events and functional signaling pathways related to diseases. However, the precise function and regulatory network of GS28 in different disease models are still unclear.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2023)
Article
Plant Sciences
Qingchen Rui, Xiaoyun Tan, Feng Liu, Yanbin Li, Xin Liu, Bingxuan Li, Junxia Wang, Huiting Yang, Lixin Qiao, Tingting Li, Shuo Fang, Rongrong Gao, Wang Wang, Sebastian Y. Bednarek, Yiqun Bao
Summary: SYP31 and SYP32 play roles in modulating Golgi morphology and pollen development by regulating protein trafficking and Golgi structure, showing partially redundant functions in pollen development.
Article
Biochemistry & Molecular Biology
Ting Ma, En Li, Lu-Shen Li, Sha Li, Yan Zhang
Summary: Gametophyte development is crucial for plant reproduction and seed yield, with the Arabidopsis YKT61 protein playing an essential role in both male and female gametogenesis. Loss of YKT61 function results in the arrest of male gametophytic development at pollen mitosis I and degeneration of female gametophytes, highlighting the importance of YKT61 in plant reproduction.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ruihua Ye, Jiaqiang Huang, Zixu Wang, Yaoxing Chen, Yulan Dong
Summary: Selenium is an essential trace element that plays a vital biological role in the body through selenoproteins. Selenoproteins are involved in various biological processes such as oxidative stress regulation, immune response, and antioxidant defense. Both deficiency and excess selenium can cause damage to the body. Selenium intake is mainly through dietary supplements. This paper reviews the synthesis and mechanism of selenoproteins, as well as their role and mechanism in disease regulation, and prospects the future development of selenium-enriched products.
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Roni Rahimi, Israel Malek, Tali Lerrer-Goldshtein, Yoav Elkis, Irit Shoval, Avi Jacob, Sally Shpungin, Uri Nir
Summary: The study investigates the regulatory role of TMF1 in GLUT4-mediated glucose uptake and insulin-directed functioning, showing that TMF1 is essential for the formation of insulin responsive, GLUT4-containing vesicles. Absence of TMF1 leads to impaired glucose uptake, resulting in hyperglycemia and decreased body weight in mice, indicating a novel effector in insulin-regulated glucose homeostasis.
Article
Cell Biology
Simona Rossi, Michela Di Salvio, Marilisa Bali, Assia De Simone, Savina Apolloni, Nadia D'Ambrosi, Ivan Arisi, Francesca Cipressa, Mauro Cozzolino, Gianluca Cestra
Summary: Compelling evidence suggests that defects in nucleocytoplasmic transport are involved in the development of amyotrophic lateral sclerosis (ALS). Hexanucleotide repeat expansions in C9orf72, the most common genetic cause of ALS, have a widespread impact on the transport machinery that controls the distribution of proteins and RNAs. This study demonstrates that C9orf72 toxic species specifically affect the membrane trafficking route regulated by ArfGAP-1.
Review
Cell Biology
Giovanna Carra, Lidia Avalle, Laura Secli, Mara Brancaccio, Alessandro Morotti
Summary: NF-kappa B is not only a transcription factor regulating gene expression within the nucleus, but also has functions within cellular organelles. The crosstalk between NF-kappa B and cellular organelles is significant for anticancer therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Ning Zhang, Olga A. Zabotina
Summary: Glycosyltransferases (GTs) play a critical role in synthesizing various glycans in cells. Glycosylation occurs in the endoplasmic reticulum and Golgi, requiring the delivery of related enzymes to the appropriate locations. This review summarizes the protein trafficking mechanism between the endoplasmic reticulum and Golgi.
Review
Cell Biology
Luoyi Zhu, Xinxia Wang, Yizhen Wang
Summary: The novel gene FAM134B plays important roles in organelle membrane morphogenesis and signaling pathway regulation, functioning in maintaining cell homeostasis through controlling Golgi morphology and cell autophagy. It is involved in various diseases and its molecular mechanisms are being uncovered, providing insights for potential therapeutic developments.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Caraline Sepich-Poore, Zhong Zheng, Emily Schmitt, Kailong Wen, Zijie Scott Zhang, Xiao-Long Cui, Qing Dai, Allen C. Zhu, Linda Zhang, Arantxa Sanchez Castillo, Haiyan Tan, Junmin Peng, Xiaoxi Zhuang, Chuan He, Sigrid Nachtergaele
Summary: Ribosomal RNAs (rRNAs) carry various chemical modifications, including m6A, which play important roles in mRNA decoding. We identified the METTL5-TRMT112 methyltransferase complex as responsible for the m6A modification on human 18S rRNA. Loss of METTL5 disrupts gene expression and causes developmental defects in humans and mice.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Douglas R. Green
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Teresa M. Buck, Aidan Porter, Wily G. Ruiz, Dennis R. Clayton, Stephanie M. Mutchler, Evan C. Ray, Allison L. Marciszyn, Lubika J. NKashama, Arohan R. Subramanya, Sebastien Gingras, Thomas Kleyman, Gerard Apodaca, Linda M. Hendershot, Jeffrey L. Brodsky
Article
Biochemistry & Molecular Biology
Swantje Liedmann, Xueyan Liu, Clifford S. Guy, Jeremy Chase Crawford, Diego A. Rodriguez, Duygu Kuzuoglu-Ozturk, Ao Guo, Katherine C. Verbist, Jamshid Temirov, Mark J. Chen, Davide Ruggero, Hui Zhang, Paul G. Thomas, Douglas R. Green
Summary: Activated CD8(+) T lymphocytes undergo heterogeneous differentiation, and this study reveals the mechanisms involved in establishing distinct T cell fate trajectories during the first division. The researchers discovered that dynein-dependent vesicular transport polarizes active TORC1 and eIF4F complexes towards the microtubule-organizing center (MTOC) at the proximal pole, leading to polarized translation of c-myc mRNA. Furthermore, the TORC1-eIF4A complex sorts preferentially to the proximal daughter cell upon division, facilitating asymmetric c-Myc synthesis. Disrupting eIF4A activity at first division skews long-term cell fate trajectories to memory-like function. These findings highlight the importance of localized translation and protein sorting in determining T cell fate.
Review
Biochemistry & Molecular Biology
R. Luke Wiseman, Jaleh S. Mesgarzadeh, Linda M. Hendershot
Summary: Cells adapt endoplasmic reticulum quality control (ERQC) to match the diverse demands encountered during normal physiology and in disease through the unfolded protein response (UPR), which plays a crucial role in maintaining ER function.
Article
Health Care Sciences & Services
Xueyan Liu, Clifford S. Guy, Emilio Boada-Romero, Douglas R. Green, Margaret E. Flanagan, Cheng Cheng, Hui Zhang
Summary: This paper presents a novel statistical method for accurate analysis of protein co-localization in high-resolution images. It addresses the limitations of traditional methods in producing false-positive errors and being restricted to 2D images. The method shows excellent performance in simulation studies and real cell analysis, and can be applied to co-localization analysis in other disciplines.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2022)
Article
Cell Biology
Douglas R. Green
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kristine F. R. Pobre-Piza, Melissa J. Mann, Ashley R. Flory, Linda M. Hendershot
Summary: The co-chaperones BiP, ERdj4, ERdj5, and GRP170 associate with aggregation-prone peptides. In this study, the impact of Interstitial Lung Disease (ILD)-associated mutants of surfactant protein C (SP-C) on protein maturation is investigated. The authors demonstrate that the ILD mutants disrupt di-sulfide bond formation and expose aggregation-prone peptides, leading to binding with ERdj4, ERdj5, and GRP170. Furthermore, the destabilized mutant BRICHOS domain affects the insertion of its transmembrane (TM) region in the ER membrane. This study provides insights into chaperone specificity, the recognition of aggregation-prone regions, and the consequences of disease-associated mutants on protein folding.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Huan Sun, Ka Yang, Xue Zhang, Yingxue Fu, Jay Yarbro, Zhiping Wu, Ping-Chung Chen, Taosheng Chen, Junmin Peng
Summary: Chemoproteomics is a crucial platform for studying the mode of action of compounds. This study presents a pooling strategy to enhance throughput and applies it to a drug library. The findings demonstrate that pooling chemoproteomics screening is an efficient method for dissecting the molecular targets of compound libraries.
Article
Biochemical Research Methods
Suresh Poudel, David Vanderwall, Zuo-Fei Yuan, Zhiping Wu, Junmin Peng, Yuxin Li
Summary: A computational pipeline JUMPptm was presented to extract PTMs from unenriched whole proteome. The deep brain proteome of Alzheimer's disease (AD) was analyzed using JUMPptm, revealing dysregulated PTM peptides during AD progression and establishing a valuable pan-PTM profile for AD research.
Review
Multidisciplinary Sciences
Joelle Magne, Douglas R. Green
Summary: LC3-associated endocytosis (LANDO) is a noncanonical function of the autophagy machinery, which plays a role in regulating receptor recycling, neuronal protection, and immune modulation. This article discusses the regulation of LANDO and its impact on endocytosis, autophagy, and phagocytosis.
Editorial Material
Multidisciplinary Sciences
Douglas R. Green, Paul G. Thomas
Editorial Material
Cell Biology
Silvia Masciarelli, Francesco Fazi, Linda M. Hendershot
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
George Daniel Grass, Dalia Ercan, Alyssa N. Obermayer, Timothy Shaw, Paul A. Stewart, Jad Chahoud, Jasreman Dhillon, Alex Lopez, Peter A. S. Johnstone, Silvia Regina Rogatto, Philippe E. Spiess, Steven A. Eschrich
Summary: This study provides the first description of the surfaceome in penile cancer and evaluates the impact of human papillomavirus (HPV) infection on surfaceome diversity. The analysis found a diverse surfaceome within patient tumors, with a high proportion of membrane proteins and transporters. The study also identified significant differences in protein classes based on HPV status and a prognostic immunoglobulin protein called BSG/CD147.
Meeting Abstract
Biochemistry & Molecular Biology
Junmin Peng
JOURNAL OF NEUROCHEMISTRY
(2022)