Article
Biochemistry & Molecular Biology
Colleen Kelly, Matthew J. Gage
Summary: This study compares the energetic barriers and folding rates of protein refolding derived from chemical, thermal, and mechanical experiments, finding that while the free energy of refolding is equivalent, there are differences in refolding rates.
Article
Biochemical Research Methods
Morgan A. Bailey, Yun Tang, Hye-Jin Park, Michael C. Fitzgerald
Summary: A new suite of mass spectrometry-based proteomic methods has been developed for evaluating protein folding stability, using chemical and thermal denaturation approaches as well as proteolysis strategies. This study compared the advantages and disadvantages of using different techniques to characterize biological phenotypes. The results showed that TPP generated the largest number of differentially stabilized protein hits, with only a quarter of the hits detected by multiple techniques.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Applied
Smriti Shrestha, Leonie van't Hag, Victoria Haritos, Sushil Dhital
Summary: Lentils and mungbean proteins have been less studied compared to pea and soybean proteins. The proteins of lentils, mungbean, and yellow pea were extracted using alkaline extraction and isoelectric precipitation, and their molecular and higher-order structures were investigated using various methods. The extracted proteins had similar protein content but higher nitrogen solubility index compared to commercial pea and soy protein isolates. The soluble proteins of lentils and yellow pea were identified as legumin-like and vicilin-like, while mungbean proteins were dominated by vicilin-like proteins. The soluble extracts showed native structural characteristics unlike the commercial extracts, and there were also differences in secondary structure between the extracts.
Article
Plant Sciences
Eliana Isabel Fernandez Sosa, Maria Guadalupe Chaves, Alejandra Viviana Quiroga, Maria Victoria Avanza
Summary: Pigeon pea protein isolates (PPI) offer the potential for high yield of quality proteins in the food industry. The study compared the physicochemical and structural properties of albumin (ALB), globulin (GLB), and PPI at different pH levels, showing that the pH variation in protein extraction can affect the functional properties of PPI.
PLANT FOODS FOR HUMAN NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Khakhanang Wijarnprecha, Philipp Fuhrmann, Christopher Gregson, Matt Sillick, Sopark Sonwai, Derick Rousseau
Summary: This study characterized the rheological behavior of back fats from pork, beef, and lamb, and established the temperature-dependent structure-function relationship governing the texture and rheology of adipose tissue. The findings provide essential insights for replicating the functionality of adipose tissue using plant-based materials.
Article
Biochemistry & Molecular Biology
Veronika Dzupponova, Natasa Tomaskova, Andrea Antosova, Erik Sedlak, Gabriel Zoldak
Summary: Thermophilic proteins and enzymes are appealing for industrial use due to their heat and denaturant resistance. Cold denaturation of thermophilic proteins, which can significantly limit their applications, is poorly understood. This study investigates the cold denaturation of thermophilic multidomain protein translation initiation factor 2 (IF2) from Thermus thermophilus. Salt can suppress cold denaturation, with the type of salt affecting this suppression. The high interdomain frustration of IF2 may be essential for its numerous diverse contacts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dongbo Shi, Virginie Jouannet, Javier Agusti, Verena Kaul, Victor Levitsky, Pablo Sanchez, Victoria V. Mironova, Thomas Greb
Summary: The study presents gene expression profiles of distinct tissues in the mature inflorescence stem of Arabidopsis thaliana using advanced techniques like fluorescence-activated nucleus sorting and RNA sequencing. They identified over 15,000 genes with differential expression in different stem tissues, revealing tissue-specific cellular signatures and potential transcriptional regulators. The datasets generated in this study provide insights into the spatial organization of physiological processes in plants at a high resolution.
Article
Biochemistry & Molecular Biology
Takamitsu Miyafusa, Hideki Watanabe, Shinya Honda
Summary: In this study, an artificial protein AF.2A1 was developed to specifically recognize non-native IgG structures, showing potential applications in quality control of biopharmaceuticals. AF.2A1 was found to recognize an exposed hydrophobic core centered on Trp417, providing molecular rationale for its usage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biology
Nimesh Shukla, Brianna Bembenek, Erika A. Taylor, Christina M. Othon
Summary: This study investigates the biopreservation and hydration effects of trehalose, maltose, and gentiobiose on proteins. It is found that despite differences only in their glycosidic bonds, these disaccharides exhibit varying degrees of stabilization against thermal denaturation due to differences in their preferential exclusion.
Article
Biodiversity Conservation
Samuel Barton, Daniel Padfield, Abigail Masterson, Angus Buckling, Nicholas Smirnoff, Gabriel Yvon-Durocher
Summary: Several experimental studies have shown that phytoplankton can adapt rapidly to warmed environments. However, these studies often use different experimental techniques, limiting our ability to compare thermal adaptation across different species. In this study, simultaneous long-term warming experiments were conducted on three phylogenetically diverse species of marine phytoplankton, revealing varying levels of thermal adaptation. Synechococcus sp. displayed the greatest improvement in fitness and thermal tolerance, Ostreococcus tauri showed some improvement but to a lesser extent, and Phaeodoactylum tricornutum showed no signs of adaptation. These findings can enhance our understanding of how phytoplankton communities may change in response to warming and the potential biogeochemical implications.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Thermodynamics
Zhi Wang, Jian Wang
Summary: This study investigated the decomposition and fire behaviors of two commonly used cables using a calorimeter. The effects of cable type, incident heat flux, and thermal aging on fire characteristics were analyzed. Differences in ignition properties and fire risk between the two cables were attributed to their compositions and structures.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Chemistry, Physical
Elham Ziaee, Behzad Shareghi, Sadegh Farhadian
Summary: BSA, as a carrier protein, plays important roles in maintaining extracellular fluid volume stability and interacting with polyamines such as spermine. The binding of spermine to BSA alters its structure and significantly affects its fluorescence properties, indicating a stabilizing effect of spermine on BSA.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Microbiology
Eric C. Keen, JooHee Choi, Meghan A. Wallace, Michelle Azar, Carlos R. Mejia-Chew, Shail B. Mehta, Thomas C. Bailey, Lindsay J. Caverly, Carey-Ann D. Burnham, Gautam Dantas
Summary: Nontuberculous mycobacteria, particularly Mycobacterium avium complex (MAC), are a growing threat to global public health, causing diverse infections that are often multidrug resistant and deadly. Through genomic analysis, distinct clinically relevant genes were identified in different MAC species and M. avium isolates from various sources, shedding light on the potential clinical implications of these pathogens. The study also revealed the presence of similar MAC strains in patients from different regions and decades, suggesting community acquisition from stable reservoirs, and the discovery of a novel MAC species.
Article
Materials Science, Ceramics
Yazhu Dong, Zhiyong Zhou, Ruihong Liang, Xianlin Dong
Summary: This study establishes a relationship between phase structures and temperature stability by preparing and studying polycrystalline ferroelectrics. The results show that the tetragonal phase is not sensitive to temperature, providing a new strategy for improving the temperature stability of ceramics. This work offers insights for the design of high-temperature piezoelectric ceramics in the future.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2022)
Article
Construction & Building Technology
Jinhua Hu, Yingdong He, Qiquan Wang, Boyang Wang, Xiaoli Hao, Nianping Li, Wei Yin, Lifang Liu
Summary: This study compared occupant thermal needs under different heating modes and explored the correlation among ambient environments, occupant responses, and occupant thermal needs. The results showed that the natural ventilation and fee-charged heating modes had similar cool indoor environments, while the fee-free heating mode had a warm indoor environment. Occupants exhibited different adaptive behaviors under different heating modes, and their needed temperatures were similar across modes. The fee-charged heating mode effectively reduced AC-use behaviors and saved energy.
JOURNAL OF BUILDING ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Sonali Rohamare, Sushama Gaikwad, Dafydd Jones, Varsha Bhavnani, Jayanta Pal, Ranu Sharma, Prathit Chatterjee
PROCESS BIOCHEMISTRY
(2015)
Article
Biophysics
Prathit Chatterjee, Neelanjana Sengupta
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2012)
Article
Chemistry, Physical
Prathit Chatterjee, Sayan Bagchi, Neelanjana Sengupta
JOURNAL OF CHEMICAL PHYSICS
(2014)
Article
Multidisciplinary Sciences
Jaya C. Jose, Prathit Chatterjee, Neelanjana Sengupta
Article
Chemistry, Medicinal
Priti Roy, Brataraj Ghosh, Prathit Chatterjee, Neelanjana Sengupta
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2019)
Article
Chemistry, Multidisciplinary
Prathit Chatterjee, Myung Keun Cho, Huong T. D. Bui, Sihyun Ham
Summary: This study reveals a two-stage mechanism of Tau43-Aβ42 and Tau43-Tau43 dimerization through atomistic molecular dynamics simulations and thermodynamics characterizations, identifying the forces driving monomers approach and formation of compact structures, as well as the roles of charged and hydrophobic residues in this process.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Prathit Chatterjee, Thi-Diem Le, Huong T. D. Bui, Myung Keun Cho, Sihyun Ham
Summary: Recent research in Alzheimer's disease has focused on understanding the mechanisms behind the formation of neurofibrillary tangles and senile plaques, particularly the role of the Tau protein. Molecular dynamics simulations have helped identify factors responsible for the aggregation propensity of Tau43, shedding light on potential drug design avenues for early AD treatment.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2021)