Article
Neurosciences
Jiyeon Seo, Hongik Hwang, Yuri Choi, Sunmin Jung, Jung-Hwa Hong, Bong-June Yoon, Hyewhon Rhim, Mikyoung Park
Summary: Many psychiatric disorders are associated with deficits in cognitive functions and synaptic plasticity, which are linked to abnormal lipid modifications of neuronal proteins. Cyclin Y (CCNY), located in the postsynaptic compartment, plays an inhibitory role in synaptic plasticity and spatial learning through myristoylation and palmitoylation.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Genetics & Heredity
Elizabeth H. Finn, Tom Misteli
Summary: The organization of the human genome within the cell nucleus is non-random, with interactions between genomic loci depending on their location. These interactions are cell-type specific and correlated with local chromatin type.
Article
Multidisciplinary Sciences
Sabine M. Ulamec, Roberto Maya-Martinez, Emily J. Byrd, Katherine M. Dewison, Yong Xu, Leon F. Willis, Frank Sobott, George R. Heath, Patricija van Oosten Hawle, Vladimir L. Buchman, Sheena E. Radford, David J. Brockwell
Summary: In this study, the authors characterized the impact of amino acid substitution on alpha-synuclein aggregation. They found that residues 38 and 42 within the P1 region of alpha-synuclein influence amyloid formation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jeffrey A. Julien, Alain Rousseau, Thomas V. Perone, David M. Lagatta, Chan Hong, Kyle T. Root, Soohyung Park, Rene Fuanta, Wonpil Im, Kerney Jebrell Glover
Summary: This study describes a reliable preparation method for full-length S-alkylated caveolin-1 and investigates the effects of S-alkylation on the protein's global helicity and local regions. It is found that S-alkylation has minor effects on the global helicity but alters the helicity in certain local regions. Additionally, the study reveals that lipidation regulates the topological promiscuity of the C-terminal domain, resulting in a deeper disposition within the bilayer.
Article
Biochemistry & Molecular Biology
Jeffrey A. Julien, Alain Rousseau, Thomas V. Perone, David M. Lagatta, Chan Hong, Kyle T. Root, Soohyung Park, Rene Fuanta, Wonpil Im, Kerney Jebrell Glover
Summary: This study demonstrates a reliable method for preparing full-length S-alkylated Caveolin-1 and reveals that S-alkylation has minimal impact on the global helicity of the protein, but can alter local regions. Furthermore, the study shows that lipidation regulates the topological properties of the C-terminal domain.
Article
Virology
Thea Kristensen, Preben Normann, Graham J. Belsham
Summary: The processing of the foot-and-mouth disease virus capsid precursor by the 3C protease is influenced by the YCPRP motif, but is not affected by the absence of the VP4 sequence. This study reveals the different mechanisms of the 3C protease in processing the capsid precursor.
Review
Biochemistry & Molecular Biology
Goro Kato
Summary: This review discusses the structural and functional aspects of Src protein and its regulatory mechanism. By reviewing nuclear magnetic resonance analyses and recent studies, the authors explore new characteristics and regulatory roles of Src protein. Finally, the new regulatory roles are integrated with the canonical model to elucidate the functions of full-length Src.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Cai Zhang, Yunshan Pei, Zeting Zhang, Lingling Xu, Xiaoli Liu, Ling Jiang, Gary J. Pielak, Xin Zhou, Maili Liu, Conggang Li
Summary: In this study, nuclear magnetic resonance spectroscopy was used to show that C-terminal truncation of alpha-synuclein alters its conformation, resulting in decreased binding of the N-terminus to membranes and molecular chaperones, which affects its cytotoxicity and aggregation. These findings provide insights into the pathological role of C-terminal truncation in Parkinson's disease.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiangyi Zhang, Yi-Hsuan Pan, Ying Chen, Chenhua Pan, Ji Ma, Chonggang Yuan, Guohua Yu, Jiyan Ma
Summary: The N-terminal polybasic region of PrP greatly influences the PrPC-to-PrPSc conversion, but is not essential for the generation of PrPSc. Both charge and the identity of amino acids in the N-terminal polybasic region are important for PrP conversion, as evidenced by the significant differences between Delta N6 and Met4-1 mutants.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Daisuke Takahashi, Kento Yonezawa, Yuki Okizaki, Jose M. M. Caaveiro, Tadashi Ueda, Atsushi Shimada, Fumio Sakane, Nobutaka Shimizu
Summary: Diacylglycerol kinases (DGKs) are multi-domain lipid kinases that regulate the levels of lipid messengers. This study investigated the structural properties of DGK alpha N-terminal (RVH and EF) domains, revealing that Ca2+ binding induces conformational changes mainly in the EF domain and identified the RVH domain as a novel Ca2+-binding domain.
Article
Biochemistry & Molecular Biology
Trevor R. Sweeney, Vidya Dhote, Ewelina Guca, Christopher U. T. Hellen, Yaser Hashem, Tatyana Pestova
Summary: Translation initiation on structured mammalian mRNAs requires the protein DHX29, which contains a unique N-terminal region and a common catalytic core. DHX29 binds to 40S subunits and has essential NTPase activity for its function. Its structure includes a dsRNA-binding domain, an alpha-helix, and a four-helix bundle that are critical for DHX29's function.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Damian M. Janecki, Agata Swiatkowska, Joanna Szpotkowska, Anna Urbanowicz, Martyna Kabacinska, Kamil Szpotkowski, Jerzy Ciesiolka
Summary: The protein PCBP2 has been shown to directly bind to the 5' terminus of p53 mRNA and is involved in both stability and translation control of p53 mRNA, especially under stress conditions. This dual function factor plays a crucial role in modulating p53 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Li Zhu, Dylan Johnson, Joseph M. Chalovich
Summary: The C-terminal 14-16 residues of human troponin T are crucial for full inactivation and prevent full activation at saturating Ca2+. The basic residues within this region are essential for the function of TnT, but the mechanism of action is still unknown.
Article
Virology
Monika Bajorek, Marie Galloux, Charles-Adrien Richard, Or Szekely, Rina Rosenzweig, Christina Sizun, Jean-Francois Eleouet
Summary: This study investigates the interaction between the matrix (M) and phosphoprotein (P) during respiratory syncytial virus (RSV) assembly and budding, revealing a direct interaction between M and P and identifying novel interaction sites on P. The findings highlight the importance of the tetrameric structural organization of P and the oligomerization domain (OD) for virus-like filament formation and VLP release.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Claudia Walliser, Kyrylo Tron, Karen Clauss, Orit Gutman, Andrei Yu. Kobitski, Michael Retlich, Anja Schade, Carlheinz Roecker, Yoav I. Henis, G. Ulrich Nienhaus, Peter Gierschik
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Cell Biology
Leslie Pomeraniec, Melissa Hector-Greene, Marcelo Ehrlich, Gerard C. Blobe, Yoav I. Henis
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Cell Biology
Keren Tazat, Melissa Hector-Greene, Gerard C. Blobe, Yoav I. Henis
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Biochemistry & Molecular Biology
Pia Paarmann, Gina Doerpholz, Juliane Fiebig, Ayelet R. Amsalem, Marcelo Ehrlich, Yoav I. Henis, Thomas Mueller, Petra Knaus
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2016)
Article
Cell Biology
Ayelet R. Amsalem, Barak Marom, Keren E. Shapira, Tal Hirschhorn, Livia Preisler, Pia Paarmann, Petra Knaus, Yoav I. Henis, Marcelo Ehrlich
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Cell Biology
Archana Varadaraj, Laura M. Jenkins, Priyanka Singh, Anindya Chanda, John Snider, N. Y. Lee, Ayelet R. Amsalem-Zafran, Marcelo Ehrlich, Yoav I. Henis, Karthikeyan Mythreye
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Cell Biology
Antony W. Burgess, Yoav I. Henis, Nancy E. Hynes, Thomas Jovin, Alexander Levitzki, Ronit Pinkas-Kramarski, Yosef Yarden
Article
Cell Biology
Keren E. Shapira, Tal Hirschhorn, Lior Barzilay, Nechama I. Smorodinsky, Yoav I. Henis, Marcelo Ehrlich
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Cell Biology
Keren E. Shapira, Marcelo Ehrlich, Yoav I. Henis
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Cell Biology
Yi Lu, Tiantian Wu, Orit Gutman, Huasong Lu, Qiang Zhou, Yoav I. Henis, Kunxin Luo
NATURE CELL BIOLOGY
(2020)
Article
Cell Biology
Keren Tazat, Leslie Pomeraniec-Abudy, Melissa Hector-Greene, Szabina Szofia Szilagyi, Swati Sharma, Elise M. Cai, Armando L. Corona, Marcelo Ehrlich, Gerard C. Blobe, Yoav Henis
Summary: Research demonstrates that ALK1, endoglin, and T beta RIII form stable complexes at the cell surface and regulate the endocytosis of TGF-beta receptors. Among these complexes, ALK1 plays a major role in affecting the endocytosis of T beta RIII and endoglin. Additionally, endoglin has minimal impact on the endocytosis of ALK1, ALK5, or T beta RII, while T beta RIII slows the internalization of ALK5 and T beta RII.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Biology
Szabina Szofia Szilagyi, Ayelet R. Amsalem-Zafran, Keren E. Shapira, Marcelo Ehrlich, Yoav Henis
Summary: Activins and bone morphogenetic proteins (BMPs) can compete for common activin type II receptors, providing a novel mechanism to balance signaling between Smad2/3 and Smad1/5/8.
Article
Cell Biology
Keren E. Shapira, Guy Shapira, Eran Schmukler, Metsada Pasmanik-Chor, Noam Shomron, Ronit Pinkas-Kramarski, Yoav Henis, Marcelo Ehrlich
Summary: The study found that cholesterol depletion regulates autophagy at three different levels, including increasing the expression of autophagy-related genes, interfering with autophagic flux, and depending on ROS accumulation and JNK activity. This has important implications for therapeutic agents targeting cholesterol reduction and in cellular settings where autophagy is involved.
CELL DEATH DISCOVERY
(2021)
Article
Plant Sciences
Hasana Sternberg, Ella Buriakovsky, Daria Bloch, Orit Gutman, Yoav Henis, Shaul Yalovsky
Summary: The study demonstrates that various Rho proteins can form self-organizing domains when co-expressed with ROPGEF3 and GAP1, which is associated with ROP-ROPGEF3 binding, reduced ROP mobility, and interaction with anionic lipids. Additionally, the ability of these domains to recruit ICR1 varies among different Rho proteins.