Article
Pharmacology & Pharmacy
Daniel Sandin, Javier Valle, Jordi Morata, David Andreu, Marc Torrent
Summary: Research has shown that antimicrobial peptides can induce tolerance in bacteria, which may be an important step in the development of antibiotic resistance. The tolerance is dependent on the type of antimicrobial peptide the bacteria are exposed to and can also affect antibiotic treatment.
Article
Biochemistry & Molecular Biology
Sathish Kumar Marimuthu, Krishnanand Nagarajan, Sathish Kumar Perumal, Selvamani Palanisamy, Latha Subbiah
Summary: Host defense peptides or antimicrobial peptides (AMPs) are short sequences of amino acids with positive or negative charges that exhibit broad-spectrum antibacterial, antiviral, and antifungal activity. AMPs rich in tryptophan, arginine, and proline are considered promising sources for the development of next-generation antibiotics. This study analyzed and classified 2924 AMPs based on their amino acid sequences and investigated their structures and stability using modeling and simulations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Vanesa Lafarga, Oleksandra Sirozh, Irene Diaz-Lopez, Antonio Galarreta, Misaru Hisaoka, Eduardo Zarzuela, Jasminka Boskovic, Bogdan Jovanovic, Rafael Fernandez-Leiro, Jaime Munoz, Georg Stoecklin, Ivan Ventoso, Oscar Fernandez-Capetillo
Summary: The presence of arginine-rich cell-penetrating peptides (CPPs) leads to displacement of RNA- and DNA-binding proteins from chromatin and mRNA, affecting nucleic acid-related reactions, potentially explaining the toxicity of these CPPs. The effects of (PR)n dipeptide repeat were found to be similar to those of protamine, a small arginine-rich protein that displaces histones during spermatogenesis.
Article
Biochemical Research Methods
Kentarou Sakamoto, Junya Michibata, Yusuke Hirai, Akiko Ide, Asuka Ikitoh, Tomoka Takatani-Nakase, Shiroh Futaki
Summary: Conjugating HAad with pyrenebutyric acid to form pBu-HAad can enhance intracellular protein delivery efficiency. Time-course and inhibitor studies suggest that anchoring HAad to the membrane with a pyrene moiety enhances peptide-membrane interaction, facilitating cytosolic translocation through membranes.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Siyuan Yu, Han Yang, Tingdong Li, Haifeng Pan, Shuling Ren, Guoxing Luo, Jinlu Jiang, Linqi Yu, Binbing Chen, Yali Zhang, Shaojuan Wang, Rui Tian, Tianying Zhang, Shiyin Zhang, Yixin Chen, Quan Yuan, Shengxiang Ge, Jun Zhang, Ningshao Xia
Summary: The authors present an intracellular protein delivery system that overcomes the limitations of ineffective endosomal escape and low serum tolerance associated with cell-penetrating peptide-mediated protein delivery. This system shows efficient delivery and therapeutic efficacy in a liver failure model, highlighting the potential of using multifunctional chimaeric peptides for developing cell-penetrating peptide-based protein delivery systems.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Giovanni Andrea Vitale, Silvia Scarpato, Alfonso Mangoni, Maria Valeria D'Auria, Gerardo Della Sala, Donatella de Pascale
Summary: Two linear proline-rich peptides (1-2) with N-terminal pyroglutamate were isolated from the marine bacterium Microbacterium sp. V1 associated with the marine sponge Petrosia ficiformis collected in Ischia Island. An integrated, untargeted MS/MS-based molecular networking and cheminformatic approach was used to detect these peptides along with others (3-8). Through extensive analysis, the planar structure and stereochemistry of the peptides were determined. Peptides 1 and 2 exhibited antioxidant properties in the FRAP assay.
Article
Biochemistry & Molecular Biology
Pramod Shah, Chien-Sheng Chen
Summary: Antimicrobial peptides (AMPs) show promise as alternatives to combat various pathogenic microorganisms, with marine AMPs such as Polyphemusin-I targeting nucleic acid-related proteins. Studies have identified intracellular protein targets of Polyphemusin-I and revealed differences in protein targets between marine and terrestrial AMPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Makoto Ozaki, Shuhei Yoshida, Takaaki Tsuruoka, Kenji Usui
Summary: The research team developed a system to directly produce gold nanoparticles in cells by intracellular mineralization at lower concentration using a peptide with cell-penetrating and gold ion-binding sequences. They were able to control the morphology of the gold nanostructures by altering the peptide structures.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yan Zhao, Haolin Jiang, Jiazhen Yu, Luyao Wang, Juanjuan Du
Summary: Currently, the clinical application of protein/peptide therapeutics is limited to the extracellular spaces, mainly due to the endosomal entrapment of internalized proteins/peptides. This study presents a strategy to design peptides that enable the delivery from endosomes to cytosol by extending the histidine switch principle. By replacing the Arg/Lys residues in cationic cell-penetrating peptides (CPPs) with histidine, the researchers obtained peptides with pH-dependent membrane-perturbation activity, mimicking the endosomal escape of CPPs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Qi Wang, Zhouxian Li, Jiahua Zhou, Yan Wang, Keyun Wang, Hongqiang Qin, Mingliang Ye
Summary: Protein methylation, especially on arginine and lysine residues, plays a critical role in cellular processes such as RNA splicing and DNA repair. A new chemical strategy has been developed to deplete histidine-containing peptides and improve the identification of low-abundance arginine/lysine methylpeptides. This method led to a 50% increase in methylation identifications, with a total of 333 forms identified in this study.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Medicinal
Rikeshwer Prasad Dewangan, Devesh Pratap Verma, Neeraj Kumar Verma, Ankit Gupta, Garima Pant, Kalyan Mitra, Saman Habib, Jimut Kanti Ghosh
Summary: In this study, a new type of proline-rich peptide mimetic was designed and synthesized. These lipopeptides exhibited significant antimicrobial activity against resistant bacteria and demonstrated resistance against trypsin digestion. They also showed synergistic antimicrobial activity when combined with tested antibiotics. The lipopeptides were found to interact with the bacterial ribosome and inhibit protein synthesis. Furthermore, they demonstrated superior clearance of bacteria compared to a conventional antibiotic.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Jing Wu, Yuanyuan Sun, Xiang Huan, Chun Cui
Summary: Some arginyl dipeptides and gamma-glutamyl peptides have been identified as salt and umami enhancers. In this study, a series of gamma-glutamyl peptides containing Arg were synthesized and evaluated for their taste-enhancing effects. The results showed that certain peptides had significant effects in increasing both saltiness and umaminess, especially at a high solid concentration of 30%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Engineering, Environmental
Nadia A. Samak, Mohamed S. Selim, Zhifeng Hao, Jianmin Xing
Summary: In this study, cyclic dodecapeptides rich in Arg-Trp were synthesized and immobilized on an RGO/MnO2 nanocomposite, showing excellent antimicrobial activity and ability to degrade biofilms.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Materials Science, Biomaterials
Si-Yi Chen, Xiao-Xue Xu, Xin Li, Ning-Bo Yi, Shi-Zhuo Li, Xing-Cheng Xiang, Dong-Bing Cheng, Taolei Sun
Summary: This article summarizes the uptake pathway of intracellular delivery vehicles for macromolecule drugs, emphasizing the importance of optimizing drug delivery systems, maintaining biological activity, improving transport efficiency, and discovering new therapies.
BIOMATERIALS SCIENCE
(2022)
Article
Cell Biology
Omar G. Klinsky, Paula A. Wetten, Emilia Zanni-Ruiz, Martin A. Pavarotti, Maria Victoria Berberian, Marcela A. Michaut
Summary: In this study, the potential of cell-penetrating peptides (CPP) as biotechnological tools for delivering molecules into oocytes was assessed, and the functionality of permeable tetanus toxin during cortical reaction was evaluated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)