Article
Chemistry, Analytical
Indra Memdi Khoris, Fahmida Nasrin, Ankan Dutta Chowdhury, Enoch Y. Park
Summary: This study proposes a dual-approach aptasensor for highly sensitive early-stage diagnosis of dengue virus NS1 protein. The developed biosensor shows high sensitivity and good selectivity with colorimetric and electrochemical detection methods.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Iveta Vilimova, Igor Chourpa, Stephanie David, Martin Souce, Katel Herve-Aubert
Summary: This study proposes a rapid and efficient protocol for the generation of magnetic nanoprobes to capture miR-155, aiming to increase its concentration. The nanoprobes were formulated by functionalizing the surface of superparamagnetic iron oxide nanoparticles with a miR sequence complementary to the target miR-155. The affinity of the nanoprobes to the target miR was demonstrated, and their stability after incubation in serum was confirmed.
Article
Chemistry, Analytical
Xiaojun Wang, Jingru Yang, Yiming Xie, Guosong Lai
Summary: This study develops a novel colorimetric biosensing method for kanamycin antibiotic detection by utilizing a target biorecognition reaction to induce the self-assembly of G-quadruplexes and the aggregation of gold nanoparticles (Au NPs). The method has a wide linear range and ultrahigh sensitivity, and shows satisfactory results in dose-recovery experiments with real samples.
Article
Biophysics
Myoungro Lee, Seong Jun Park, Gahyeon Kim, Chulhwan Park, Min-Ho Lee, Jae-Hyuk Ahn, Taek Lee
Summary: In this study, a capacitance-based electrical biosensor was developed to detect exosomes in undiluted serum samples without pretreatment. The biosensor utilized a DNA aptamer for CD63 protein detection and MoS2 nanoparticles to enhance the electrical sensitivity. The interdigitated micro-gap electrode system efficiently amplified the electrical signal for exosome detection, offering high selectivity and sensitivity. The biosensor exhibited linearity between the capacitance signal and exosome concentration in serum, with a detection limit of 2192.6 exosomes/mL.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Energy & Fuels
Carolina Martins Primo, Edervaldo Buffon, Nelson Ramos Stradiotto
Summary: The study developed a simple, sensitive, and low-cost electroanalytical method for copper determination in aviation biokerosene using an electrode modified with a composite made up of multi-walled carbon nanotubes and pectin. The proposed electrode exhibited good accuracy for copper determination and no interference from matrix effect, making it suitable for monitoring copper in aviation biokerosene. The results were validated by recovery assays and compared with flame atomic absorption spectroscopy, confirming the reliability of the proposed electrode.
Article
Chemistry, Analytical
Yu Muto, Gen Hirao, Tamotsu Zako
Summary: The study presented a AuNP-based signal-amplifying sandwich biosensor for detecting target molecules without the need for conformational changes in ligands. Thrombin was used as a model target, triggering an extension reaction via hybridization of two probes and subsequently RNA synthesis initiation. The amplified RNA drives aggregation or dispersion of the AuNPs, leading to observable color differences in the solution. This detection system can be universally applied to a wide range of ligands due to its ligand-independent design.
Article
Chemistry, Analytical
Ji Zheng, Dayong Li, Jin Jiao, Chengjie Duan, Youjing Gong, Hai Shi, Zhongyun Wang, Yang Xiang
Summary: Researchers have successfully developed a biomimetic recognition strategy inspired by jellyfish tentacles, with multivalent aptamers, to efficiently capture and release tumor cells. The strategy allows for high viability of released cells by degrading biomimetic tentacles with DNase I, simplifying the release process and reducing unnecessary costs.
Review
Biotechnology & Applied Microbiology
Chitra Padmakumari Kurup, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
Summary: Ultra-sensitive biosensors require signal enhancement strategies to achieve low detection limits, with nanotechnology emerging as a strong contender for amplifying signals. Nanomaterials, including gold, carbon, graphene, carbon nanotubes, quantum dots, and metal-organic frameworks, have been utilized as signal amplification components in aptamer-based electrochemical biosensors. These advancements contribute to the development of biosensors for scientific research.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Shaoguang Li, Hongxing Li, Xun Li, Man Zhu, Hui Li, Fan Xia
Summary: The study introduces a calibration-free O-N approach to enhance the sensitivity of electrochemical DNA sensors by using two types of redox reporters, achieving accurate nucleic acid measurement independent of variations in probe counts and lengths.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yin Bao, Kaiwei Cai, Yichen Liu, Bingling Li
Summary: The development of an accurate and practical electrochemical biosensor for the detection of kanamycin residues in water samples is described. The sensor demonstrates a simple construction method, good selectivity, and stability, and can provide rapid or real-time detection of kanamycin residues in water samples.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Minjun Yang, Christopher Batchelor-McAuley, Enno Kaetelhoen, Richard G. Compton
Summary: This study presents a new method for characterizing diffusional transport within porous layers, which is important for designing new amperometric sensors and electro-catalytic electrodes. By analyzing the current-time transients and utilizing the diffusion indicator alpha, the research reveals bottlenecks to transport caused by spherical blocks and the timescale at which this occurs. This method also provides a way to estimate the thickness of the porous layer.
APPLIED MATERIALS TODAY
(2021)
Article
Engineering, Biomedical
Xiaomin Li, Licheng Yu, Chuangnian Zhang, Xiaoyan Niu, Mengjie Sun, Zichao Yan, Wei Wang, Zhi Yuan
Summary: A pH-responsive gold nanoassembly was designed to improve the accumulation and penetration of nanomedicines in tumor, resulting in enhanced therapeutic efficacy.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, Na Li, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, Chuanlai Xu, Yang Yang, Bi-Feng Yuan, Quan Yuan, Chao Zhang, Zhi Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan, Chunhai Fan
Summary: Nucleic acids are natural biopolymers that play central roles in storing, encoding, transmitting and expressing genetic information. The field of nucleic acids analysis has rapidly advanced with technological breakthroughs, leading to the development of new analysis methods. However, there are also challenges and further research needed in this area.
SCIENCE CHINA-CHEMISTRY
(2021)
Review
Biochemical Research Methods
Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: The discovery of new molecular biomarkers of cancer and the development of diagnostic devices using these biomarkers have greatly improved the clinical analysis and outcomes of cancer. Liquid biopsy sensors, which use aptamers for the detection of cancer biomarkers in body fluids, provide fast and inexpensive non-invasive screening tools. Incorporating aptamers in electrochemical sandwich biosensors, known as ELASA assays, has shown promise in improving detection schemes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yong-Qiang He, Ying Chen, Xian-Zhu Meng, Hong -Chao Yi, Hui-Wen Gu, Xiao-Li Yin
Summary: A visual and smartphone-integrated aptasensor for rapid Staphylococcus aureus determination was developed using Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. The assay quantified the released DNAzymes via Exo III-assisted recycling reaction and analyzed them with a smartphone and UV-Vis spectrophotometer. This method exhibited good analytical performance, low cost, and portability, making it a rapid point-of-care test method with great potential in food safety control.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xiaoxue Cheng, Yurong Yan, Xueping Chen, Jiaxin Duan, Decai Zhang, Tiantian Yang, Xiaolong Gou, Min Zhao, Shijia Ding, Wei Cheng
Summary: This study explored the cleavage properties of Cas12a system on nanomaterial surfaces using gold nanoparticles and graphene oxide. The results indicated that graphene oxide exhibited better quenching ability and Cas12a-induced fluorescence recovery compared to gold nanoparticles, and double-stranded DNA probes with staggered ends led to superior cleavage efficiency.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Lina Kang, Jiaxin Duan, Fang He, Jie Teng, Jia Li, Tiantian Yang, Nini Luo, Jie Zhao, Shijia Ding, Wei Cheng
Summary: The innovative fluorescence biosensing methodology developed for detecting BCR-ABL1 mRNA shows high sensitivity and specificity, with a limit of detection at 0.5 aM and a linear range from 2 aM to 200 fM. This method was successfully used to directly detect as low as 0.001% e13a2 transcript isoforms from complex genomic RNA extraction. Compared to clinical routine, the thermostatic reaction process eliminates the need for additional reverse transcription.
Article
Chemistry, Analytical
Peihang Yu, Tiantian Yang, Decai Zhang, Lulu Xu, Xiaoxue Cheng, Shijia Ding, Wei Cheng
Summary: This new telomerase activity detection method based on CRISPR technology is fast, sensitive, and has a wide linear regression range, offering great potential for cancer diagnosis in a one-step process.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Decai Zhang, Yurong Yan, Xiaoxue Cheng, Tiantian Yang, Xingrong Li, Shijia Ding, Xiuming Zhang, Wei Cheng
Summary: This study investigated the trans-cleavage activity of CRISPR-Cas12a and revealed the influence of PAM nucleotides on its activation. The enhanced strand displacement mechanism by Cas12a to promote crRNA invasion was also demonstrated. Additionally, a modular CRISPR-Cas12a-based signal reporting system was developed for universal biosensing, enabling the detection of various analytes with high sensitivity and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Husun Qian, Yixin Fu, Minkang Guo, Yu Chen, Dian Zhang, Yu Wei, Fangfang Jin, Qian Zeng, Yange Wang, Chengsen Chai, Shijia Ding, Wei Cheng, Tingmei Chen
Summary: Chimeric antigen receptor T (CAR-T) cell therapy has limited efficacy in solid tumors, but a non-genetic macrophage cell engineering strategy using glycan metabolic labeling and click reaction shows promise. The engineered macrophages can effectively target tumor cells and induce cytotoxicity, while also reprogramming the immune microenvironment.
Article
Medicine, Research & Experimental
Husun Qian, Ting Zhou, Yixin Fu, Minkang Guo, Wu Yang, Dian Zhang, Wenli Fang, Mengli Yao, He Shi, Chengsen Chai, Wei Cheng, Shijia Ding, Tingmei Chen
Summary: This study developed an efficient delivery system for siRNA and CpG ODNs, which facilitated the polarization of tumor-associated macrophages (TAMs) towards the immunomodulatory M1 phenotype, resulting in superior antitumor efficacy.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Ningke Fan, Xintong Bian, Meng Li, Junman Chen, Haiping Wu, Qiling Peng, Huijie Bai, Wenqian Cheng, Liangsheng Kong, Shijia Ding, Siqiao Li, Wei Cheng
Summary: The study developed a customized RNA nanococoon, capable of tumor-specific recognition and spatial-controlled activation of Cas13a, leading to effective silencing of EGFRvIII mRNA for synergistic cancer therapy.
Article
Biophysics
Wenqian Cheng, Zi Lin, Lina Zhao, Ningke Fan, Huijie Bai, Wei Cheng, Min Zhao, Shijia Ding
Summary: An on-off nonenzymatic and ultrasensitive electrochemiluminescence biosensing platform for detecting BCR-ABL fusion gene has been developed based on CeO2/MXene heterojunction and configuration-entropy driven dual-toehold strand displacement reaction. This platform achieves wide concentration variation and low limit of detection, providing new insights for molecular diagnosis of chronic myelogenous leukemia in clinical practice.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Nini Luo, Yue Shu, Chuanlong Zhu, Xiaoxue Cheng, Lixue Chen, Jiang Wang, Rui Liu, Huangxian Ju, Wei Cheng, Qianfeng Xia
Summary: In this study, a biosensor based on UiO-66 was developed to directly detect the phosphorylation levels of target proteins. Optimization of the pH of the incubation buffer and the NaOH concentration in the washing solution enhanced the specificity and sensitivity of the method. This method can analyze the phosphorylation level of target proteins in complex samples without affecting the protein structure.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemical Research Methods
Yang Song, Ke Gao, Xiaoying Cai, Wei Cheng, Shijia Ding, Decai Zhang, Shixiong Deng
Summary: A controllable crRNA self-transcription aided dual-amplified CRISPR-Cas12a strategy, named CST-Cas12a, has been developed for highly sensitive and specific biosensing of flap endonuclease 1 (FEN1). This strategy utilizes a branched DNA probe as a hydrolysis substrate for FEN1, and generates double-stranded DNA (dsDNA) containing a T7 promoter and crRNA transcription template. With the help of T7 RNA polymerase, abundant crRNA is produced and assembled with Cas12a to form a Cas12a/crRNA complex. This complex can be activated by a dsDNA trigger and unlocks the fluorophore-quencher reporter cleavage, enabling highly efficient dual signal amplification and near-zero background. Under optimized conditions, the CST-Cas12a method allows highly sensitive biosensing of FEN1 activity with excellent specificity in the presence of other interfering enzymes. Furthermore, this strategy has been successfully applied to FEN1 biosensing in complex biological samples.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tiantian Yang, Juan Li, Decai Zhang, Xiaoxue Cheng, Jia Li, Xiaolin Huang, Shijia Ding, Ben Zhong Tang, Wei Cheng
Summary: This study demonstrates a tunable G-quadruplex (G4) structure that enhances CRISPR/Cas12a detection and explores its mechanism. Two different strategies using the G4 structure are designed to achieve ultra-sensitive visual nucleic acid detection of Group B Streptococci.
Article
Chemistry, Multidisciplinary
Mingxuan Song, Juanxia Xing, Huan Cai, Xin Gao, Chunyang Li, Changjin Liu, Xinmin Li, Xuhuai Fu, Shijia Ding, Wei Cheng, Rui Chen
Summary: In this study, a colorimetric catalytic nanocomplex (HRP@ZIF-8)(3)@PDA@HRP was synthesized using a pomegranate-inspired structure, and its potential for ultrasensitive colorimetric LFIA of cTnI was demonstrated. The nanocomplex exhibited high loading efficiency and catalytic activity, attributed to the shell-by-shell overgrowth of the porous ZIF-8 scaffold. Furthermore, the polydopamine layer on the surface of the nanocomplex enhanced the colorimetric signal brightness and acted as a flexible scaffold for HRP immobilization. The developed platform achieved an ultrasensitive colorimetric test strip assay with high sensitivity and comparable performance to chemiluminescence immunoassay.
Article
Chemistry, Analytical
Bo Shen, Li Li, Changjin Liu, Xinmin Li, Xinyu Li, Xiaoxue Cheng, Haiping Wu, Tiantian Yang, Wei Cheng, Shijia Ding
Summary: An ultrasensitive electrochemiluminescent (ECL) biosensor was constructed for accurate detection of exosomal microRNAs (exomiRNAs), using a three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI). The biosensor achieved a limit of detection down to 273.20 aM and successfully discriminated breast cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weicheng Shi, Yao Gong, Decai Zhang, Tiantian Yang, Ming Yi, Jingyi Tan, Shijia Ding, Wei Cheng
Summary: In this study, a strategy based on rolling circle amplification and CRISPR/Cas14a was developed for highly specific detection of BRAF V600E mutation in fine-needle biopsy samples. The method showed outstanding specificity and discrimination ability, and was successfully applied in clinical samples. This approach provides a novel method for the diagnosis and treatment of cancer.
ANALYTICAL METHODS
(2022)