Article
Chemistry, Analytical
Honghong Wang, Shuhui Wang, Hui Wang, Yuanwen Liang, Zhengping Li
Summary: In this study, a reliable, amplification-free, and sensitive strategy for the detection of telomerase activity based on the self-extension of telomerase and trans-cleavage of CRISPR/Cas12a is presented. The method allows the detection of telomerase activity in very few cancer cells without complex primer design and any nucleic acid amplification, and it exhibits high reliability and specificity in distinguishing tumor cells from normal cells.
Article
Chemistry, Multidisciplinary
Siyu Chen, Rujia Wang, Shuang Peng, Shiyi Xie, Chunyang Lei, Yan Huang, Zhou Nie
Summary: In this study, the CRISPR-Cas system was engineered as a versatile live-cell biosensing system for monitoring diverse intracellular biomolecules. By leveraging the trans-cleavage activity of CRISPR-Cas12a, this system enabled sensitive sensing of various biomolecules such as telomerase, ATP, and microRNA-21.
Article
Biophysics
Tian Tian, Zhiqiang Qiu, Yongzhong Jiang, Debin Zhu, Xiaoming Zhou
Summary: This study introduces a highly efficient dual-gene detection technique based on the orthogonal DNA/RNA collateral cleavage mechanism of the Cas12a/Cas13a system. The technique allows simultaneous detection of multiple amplified products and can be implemented on a portable device for accurate infectious disease screening in resource-limited settings.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Fengqin Li, Junru Li, Weiqiang Yang, Shuai Yang, Congshuo Chen, Ling Du, Junyang Mei, Qianyun Tang, Xiaojing Chen, Chi Yao, Dayong Yang, Xiaolei Zuo, Peifeng Liu
Summary: The trans-cleavage property of CRISPR-Cas12a system is utilized for disease diagnosis. The effect of different local densities of Framework-Hotspot reporters (FHRs) on the cleavage activity of Cas12a is investigated. A modular sensing platform is constructed with target recognition by CRISPR-Cas12a and signal transduction by FHR. This platform enables sensitive and rapid detection of pathogen nucleic acids and tumor protein markers without pre-amplification.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Hailong Lv, Jian Wang, Jian Zhang, Yijian Chen, Lei Yin, Dian Jin, Dayong Gu, Huailong Zhao, Yong Xu, Jin Wang
Summary: Despite the great potential of CRISPR-Dx technology in diagnostic sensitivity, specificity, convenience, and portability, the lack of standardized definition of Cas trans-cleavage enzymatic units has impeded the development of the industry. By optimizing the reaction systems for Cas12a and defining its trans-cleavage units (transU), researchers can now have a reference point for standardization and further research in the field.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Decai Zhang, Yurong Yan, Xiaoxue Cheng, Tiantian Yang, Xingrong Li, Shijia Ding, Xiuming Zhang, Wei Cheng
Summary: This study investigated the trans-cleavage activity of CRISPR-Cas12a and revealed the influence of PAM nucleotides on its activation. The enhanced strand displacement mechanism by Cas12a to promote crRNA invasion was also demonstrated. Additionally, a modular CRISPR-Cas12a-based signal reporting system was developed for universal biosensing, enabling the detection of various analytes with high sensitivity and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Chenyu Cui, Cia-Hin Lau, Lok Ting Chu, Hoi Kwan Kwong, Chung Tin, Ting-Hsuan Chen
Summary: In this study, a multimodal detection of FEN1 activity based on CRISPR/Cas12a was developed. The method allows for efficient signal amplification and achieves a low limit of detection, making it a promising approach for cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xiaolong Chen, Gaihua Cao, Jiajin Zhang, Yuanyi Deng, Xiaogang Luo, Mei Yang, Danqun Huo, Changjun Hou
Summary: Enzymes are essential components of organisms, ensuring normal life activities. Abnormal enzyme activity can lead to dysfunction and disease. Establishing simple, sensitive, and timely detection of enzyme activity is crucial for biomedical testing and disease diagnosis and treatment. We developed a strategy for detecting T4 PNK, telomerase, or APE1 activity using enzyme extends activators to unlock CRISPR/Cas12a's ssDNase activity (EdU-CRISPR/Cas12a), achieving low detection limits through fluorescence and lateral flow assay methods. Our approach has the potential to greatly improve enzyme activity detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Ming Yi, Yao Gong, Qian Zhan, Yulian Dai, Tiantian Yang, Xiaoxue Cheng, Shijia Ding, Bing Gu, Wei Cheng, Decai Zhang
Summary: An isothermal, one-pot toolbox based on CRISPR-Cas12a collateral cleavage capability (OPT-Cas) is proposed for highly sensitive and selective determination of terminal deoxynucleotidyl transferase (TdT) activity. The toolbox allows simple but high-sensitive quantification of TdT activity with low detection limits and achieves extraordinary selectivity. Furthermore, it has been successfully used for the detection of TdT in complex matrices and accurate determination of TdT activity in acute lymphoblastic leukemia cells.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Guojun Wei, Zhibing Peng, Jingsong Liu, Kun Yang, Chenglong Zhao, Wei Xie, Tianwen Huang, Jiafeng Liu, Jin Li, Gang An
Summary: A novel CRISPR-Cas12a-based fluorescent sensing platform was proposed for sensitive and reliable analysis of telomerase activity. This platform can distinguish both the amounts of telomerase and the length of telomerase extension products. The introduction of the average telomerase activity index shows potential for accurate cancer reporting and osteosarcoma staging.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biophysics
Jinjoo Han, Jiye Shin, Eun Sung Lee, Byung Seok Cha, Seokjoon Kim, Youngjun Jang, Seokhwan Kim, Ki Soo Park
Summary: In this study, a Cas12a-based multiplex detection system was developed to simultaneously detect two genes using a single Cas protein. The system demonstrated high sensitivity and specificity in detecting high-risk human papillomavirus (HPV) genes within a short time. This system has potential applications in point-of-care testing and the detection of various nucleic acid biomarkers.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Qian Li, Zhi-Ling Song, Yuxi Zhang, Lina Zhu, Qian Yang, Xingfu Liu, Xufeng Sun, Xuxu Chen, Rongmei Kong, Gao-Chao Fan, Xiliang Luo
Summary: A synergistic activator effect is discovered, where the incorporation of two short ssDNA activators can promote the trans-cleavage of CRISPR/Cas12a. This finding provides deeper insight into CRISPR/Cas12a and may facilitate its expanded application and exploration of other CRISPR/Cas systems.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Aleksandr V. Ivanov, Irina V. Safenkova, Anatoly V. Zherdev, Yi Wan, Boris B. Dzantiev
Summary: Biosensors based on Cas12 endonuclease exhibit high specificity in pathogen detection. A comparison was conducted on single-stranded (ss) DNA probes, namely polyA, polyC, and polyT, with different lengths immobilized on streptavidin-covered magnetic particles (MPs). The most effective probe was found to be polyC, followed by polyT and polyA. These findings provide valuable insights for the detection of viruses, bacteria, and other DNA/RNA-containing analytes using Cas12a-based methods.
Article
Biophysics
Chenqi Niu, Chuyi Wang, Fan Li, Xiang Zheng, Xinhui Xing, Chong Zhang
Summary: The Molecular Radar is a versatile diagnostic strategy that allows for rapid and convenient detection of small molecules using the CRISPR Cas12a system and DNA aptamer technology. This method shows potential for on-site molecular diagnostics in point-of-care settings, providing high specificity and sensitivity for target molecule detection. Additionally, the unique characteristics of CRISPR-Cas12a demonstrated in this study contribute to a better understanding of Cas12a and offer guidance for further research on CRISPR-Cas12a-based biosensors.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Qian Wang, Yaqi Liu, Jixian Yan, Yunqing Liu, Chaomin Gao, Shenguang Ge, Jinghua Yu
Summary: This study proposed a new ultrasensitive miRNA detection method based on CRISPR/Cas12a, utilizing multiple mechanisms such as a 3D DNA walker and 3D-rGO/AuPd nanoparticles to achieve high sensitivity and specificity in detection.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Ying Zheng, Siqiao Li, Daobin Han, Liangsheng Kong, Jianmin Wang, Min Zhao, Wei Cheng, Huangxian Ju, Zhongzhu Yang, Shijia Ding
Summary: This study developed an environmentally friendly and effective erGO nanofilm with dual antimicrobial mechanisms, capable of successfully inhibiting multidrug-resistant bacteria and promoting wound healing, showing great potential for medical dressing applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Xiaolong Gou, Lulu Xu, Suqing Yang, Xiaoxue Cheng, Haiping Wu, Decai Zhang, Weicheng Shi, Shijia Ding, Yuhong Zhang, Wei Cheng
Summary: A strategy based on nanocluster beacon (NCB) fluorescence was developed to identify the two isoforms of the BCR/ABL(p210) fusion gene in one-pot, which showed distinct differences in clinical manifestation, treatment effect, and prognosis risk. The fluorescence of AgNCs can be activated when they are placed in proximity to the corresponding enhancer sequences, allowing for successful identification of the two isoforms with high sensitivity and specificity. This strategy also realized isoform identification in leukemia cells and newly diagnosed CML patients within 40 minutes, providing a powerful tool to distinguish fusion gene subtypes simultaneously.
Article
Biochemistry & Molecular Biology
Jia Li, Haiping Wu, Yurong Yan, Taixian Yuan, Yue Shu, Xin Gao, Lu Zhang, Siqiao Li, Shijia Ding, Wei Cheng
Summary: Our study introduced a novel strategy, PECA, to enhance the catalytic capacity of G4/hemin DNAzyme, resulting in the development of a highly efficient HRP mimic called Z-G4/H. This innovative approach showed promising applications in sensitive gene rearrangement detection and imaging of HER2 protein dimerization on cell surfaces, indicating universal diagnostic potential in clinical settings and biomolecule interaction research.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Decai Zhang, Yurong Yan, Xiaoxue Cheng, Tiantian Yang, Xingrong Li, Shijia Ding, Xiuming Zhang, Wei Cheng
Summary: This study investigated the trans-cleavage activity of CRISPR-Cas12a and revealed the influence of PAM nucleotides on its activation. The enhanced strand displacement mechanism by Cas12a to promote crRNA invasion was also demonstrated. Additionally, a modular CRISPR-Cas12a-based signal reporting system was developed for universal biosensing, enabling the detection of various analytes with high sensitivity and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Husun Qian, Yixin Fu, Minkang Guo, Yu Chen, Dian Zhang, Yu Wei, Fangfang Jin, Qian Zeng, Yange Wang, Chengsen Chai, Shijia Ding, Wei Cheng, Tingmei Chen
Summary: Chimeric antigen receptor T (CAR-T) cell therapy has limited efficacy in solid tumors, but a non-genetic macrophage cell engineering strategy using glycan metabolic labeling and click reaction shows promise. The engineered macrophages can effectively target tumor cells and induce cytotoxicity, while also reprogramming the immune microenvironment.
Article
Medicine, Research & Experimental
Husun Qian, Ting Zhou, Yixin Fu, Minkang Guo, Wu Yang, Dian Zhang, Wenli Fang, Mengli Yao, He Shi, Chengsen Chai, Wei Cheng, Shijia Ding, Tingmei Chen
Summary: This study developed an efficient delivery system for siRNA and CpG ODNs, which facilitated the polarization of tumor-associated macrophages (TAMs) towards the immunomodulatory M1 phenotype, resulting in superior antitumor efficacy.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Ningke Fan, Xintong Bian, Meng Li, Junman Chen, Haiping Wu, Qiling Peng, Huijie Bai, Wenqian Cheng, Liangsheng Kong, Shijia Ding, Siqiao Li, Wei Cheng
Summary: The study developed a customized RNA nanococoon, capable of tumor-specific recognition and spatial-controlled activation of Cas13a, leading to effective silencing of EGFRvIII mRNA for synergistic cancer therapy.
Article
Biophysics
Wenqian Cheng, Zi Lin, Lina Zhao, Ningke Fan, Huijie Bai, Wei Cheng, Min Zhao, Shijia Ding
Summary: An on-off nonenzymatic and ultrasensitive electrochemiluminescence biosensing platform for detecting BCR-ABL fusion gene has been developed based on CeO2/MXene heterojunction and configuration-entropy driven dual-toehold strand displacement reaction. This platform achieves wide concentration variation and low limit of detection, providing new insights for molecular diagnosis of chronic myelogenous leukemia in clinical practice.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Nini Luo, Yue Shu, Chuanlong Zhu, Xiaoxue Cheng, Lixue Chen, Jiang Wang, Rui Liu, Huangxian Ju, Wei Cheng, Qianfeng Xia
Summary: In this study, a biosensor based on UiO-66 was developed to directly detect the phosphorylation levels of target proteins. Optimization of the pH of the incubation buffer and the NaOH concentration in the washing solution enhanced the specificity and sensitivity of the method. This method can analyze the phosphorylation level of target proteins in complex samples without affecting the protein structure.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemical Research Methods
Yang Song, Ke Gao, Xiaoying Cai, Wei Cheng, Shijia Ding, Decai Zhang, Shixiong Deng
Summary: A controllable crRNA self-transcription aided dual-amplified CRISPR-Cas12a strategy, named CST-Cas12a, has been developed for highly sensitive and specific biosensing of flap endonuclease 1 (FEN1). This strategy utilizes a branched DNA probe as a hydrolysis substrate for FEN1, and generates double-stranded DNA (dsDNA) containing a T7 promoter and crRNA transcription template. With the help of T7 RNA polymerase, abundant crRNA is produced and assembled with Cas12a to form a Cas12a/crRNA complex. This complex can be activated by a dsDNA trigger and unlocks the fluorophore-quencher reporter cleavage, enabling highly efficient dual signal amplification and near-zero background. Under optimized conditions, the CST-Cas12a method allows highly sensitive biosensing of FEN1 activity with excellent specificity in the presence of other interfering enzymes. Furthermore, this strategy has been successfully applied to FEN1 biosensing in complex biological samples.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tiantian Yang, Juan Li, Decai Zhang, Xiaoxue Cheng, Jia Li, Xiaolin Huang, Shijia Ding, Ben Zhong Tang, Wei Cheng
Summary: This study demonstrates a tunable G-quadruplex (G4) structure that enhances CRISPR/Cas12a detection and explores its mechanism. Two different strategies using the G4 structure are designed to achieve ultra-sensitive visual nucleic acid detection of Group B Streptococci.
Article
Chemistry, Multidisciplinary
Mingxuan Song, Juanxia Xing, Huan Cai, Xin Gao, Chunyang Li, Changjin Liu, Xinmin Li, Xuhuai Fu, Shijia Ding, Wei Cheng, Rui Chen
Summary: In this study, a colorimetric catalytic nanocomplex (HRP@ZIF-8)(3)@PDA@HRP was synthesized using a pomegranate-inspired structure, and its potential for ultrasensitive colorimetric LFIA of cTnI was demonstrated. The nanocomplex exhibited high loading efficiency and catalytic activity, attributed to the shell-by-shell overgrowth of the porous ZIF-8 scaffold. Furthermore, the polydopamine layer on the surface of the nanocomplex enhanced the colorimetric signal brightness and acted as a flexible scaffold for HRP immobilization. The developed platform achieved an ultrasensitive colorimetric test strip assay with high sensitivity and comparable performance to chemiluminescence immunoassay.
Article
Chemistry, Analytical
Bo Shen, Li Li, Changjin Liu, Xinmin Li, Xinyu Li, Xiaoxue Cheng, Haiping Wu, Tiantian Yang, Wei Cheng, Shijia Ding
Summary: An ultrasensitive electrochemiluminescent (ECL) biosensor was constructed for accurate detection of exosomal microRNAs (exomiRNAs), using a three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI). The biosensor achieved a limit of detection down to 273.20 aM and successfully discriminated breast cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weicheng Shi, Yao Gong, Decai Zhang, Tiantian Yang, Ming Yi, Jingyi Tan, Shijia Ding, Wei Cheng
Summary: In this study, a strategy based on rolling circle amplification and CRISPR/Cas14a was developed for highly specific detection of BRAF V600E mutation in fine-needle biopsy samples. The method showed outstanding specificity and discrimination ability, and was successfully applied in clinical samples. This approach provides a novel method for the diagnosis and treatment of cancer.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
