Using Directed Evolution to Improve Hydrogen Production in Chimeric Hydrogenases from Algal Species

Linking protein structural and functional change to mutation using amino acid networks

(2022) Cristina Sotomayor-Vivas et al.   PLoS One

Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii

(2022) Friedrich Fauser et al.   NATURE GENETICS

Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase

(2022) Sven T. Stripp et al.   CHEMICAL REVIEWS

Current strategies and future perspectives in biological hydrogen production: A review

(2022) Balasubramani Ramprakash et al.   RENEWABLE & SUSTAINABLE ENERGY REVIEWS

A directed genome evolution method to enhance hydrogen production in Rhodobacter capsulatus

(2022) Emma Barahona et al.   Frontiers in Microbiology

De novo determination of near-surface electrostatic potentials by NMR

(2021) Binhan Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide

(2021) Miao Tian et al.   ENZYME AND MICROBIAL TECHNOLOGY

Using continuous directed evolution to improve enzymes for plant applications

(2021) Jorge D García-García et al.   PLANT PHYSIOLOGY

Opportunities toward hydrogen production biotechnologies

(2020) James Swartz   CURRENT OPINION IN BIOTECHNOLOGY

Practically useful protein-design methods combining phylogenetic and atomistic calculations

(2020) Jonathan Weinstein et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Recombination of 2Fe-2S Ferredoxins Reveals Differences in the Inheritance of Thermostability and Midpoint Potential

(2020) Ian J. Campbell et al.   ACS Synthetic Biology

Recent advancement and strategy on bio-hydrogen production from photosynthetic microalgae

(2019) Muhammad Anwar et al.   BIORESOURCE TECHNOLOGY

How [FeFe]-Hydrogenase Facilitates Bidirectional Proton Transfer

(2019) Moritz Senger et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

(2018) Hui Li et al.   Biotechnology for Biofuels

Electrostatic Interactions in Protein Structure, Folding, Binding, and Condensation

(2018) Huan-Xiang Zhou et al.   CHEMICAL REVIEWS

Insights on the Origin of Catalysis on Glycine N-Methyltransferase from Computational Modeling

(2018) Katarzyna Świderek et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms

(2018) Sudhir Kumar et al.   MOLECULAR BIOLOGY AND EVOLUTION

Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

(2018) Adam Wegelius et al.   Energy & Environmental Science

Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives

(2018) Amel Latifi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases

(2018) Jifu Duan et al.   Nature Communications

Complex Multimeric [FeFe] Hydrogenases: Biochemistry, Physiology and New Opportunities for the Hydrogen Economy

(2018) Kai Schuchmann et al.   Frontiers in Microbiology

Hydrogen production and microbial kinetics of Clostridium termitidis in mono-culture and co-culture with Clostridium beijerinckii on cellulose

(2017) Maritza Gomez-Flores et al.   AMB Express

Using directed evolution to improve hydrogen production in chimeric hydrogenases from Clostridia species

(2016) Scott M. Plummer et al.   ENZYME AND MICROBIAL TECHNOLOGY

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Enhanced activity of Rhizomucor miehei lipase by directed evolution with simultaneous evolution of the propeptide

(2012) Jue Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production

(2011) Jonathan E. Meuser et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Demonstration of hydrogenase electrode operation in a bioreactor

(2011) E.S. Shastik et al.   ENZYME AND MICROBIAL TECHNOLOGY

Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity

(2011) Alyssa S. Bingham et al.   INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

Importance of the Protein Framework for Catalytic Activity of [FeFe]-Hydrogenases

(2011) Philipp Knörzer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Photobiological production of hydrogen gas as a biofuel

(2010) James B McKinlay et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genetic Engineering of Algae for Enhanced Biofuel Production

(2010) Randor Radakovits et al.   EUKARYOTIC CELL

Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydAΔEFG

(2010) David W. Mulder et al.   NATURE

A Cell-Free Microtiter Plate Screen for Improved [FeFe] Hydrogenases

(2010) James A. Stapleton et al.   PLoS One

Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases

(2010) James A. Stapleton et al.   PLoS One

Modeling the folding and hydrogen production of Clostridium acetobutylicum and Clostridium saccharobutylium mutants using electrostatic potential surfaces and molecular dynamics

(2009) Mark A. Plummer et al.   COMPUTATIONAL BIOLOGY AND CHEMISTRY

Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production

(2009) Christine M. English et al.   DALTON TRANSACTIONS

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Photobiological hydrogen-producing systems

(2008) Maria Lucia Ghirardi et al.   CHEMICAL SOCIETY REVIEWS