Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level

Tau liquid–liquid phase separation is modulated by the Ca 2+ ‐switched chaperone activity of the S100B protein

(2023) Guilherme G. Moreira et al.   JOURNAL OF NEUROCHEMISTRY

Elucidating the mechanism of MBD protein LLPS and its role in heterochromatin formation and transcriptional repression

(2023) Nicole Maurici et al.   BIOPHYSICAL JOURNAL

Fast synaptic vesicle docking and cluster rearrangement after short stimuli

(2023) Junxiu Duan et al.   BIOPHYSICAL JOURNAL

Initiation and modulation of Tau protein phase separation by the drug suramin

(2023) Prabhu Rajaiah Prince et al.   Scientific Reports

Nucleation and dissolution mechanism underlying amyotrophic lateral sclerosis/frontotemporal lobar dementia-linked fused in sarcoma condensates

(2023) Nathalie A. Djaja et al.   iScience

Single molecule reaction-diffusion with single photon FCS-FRET

(2022) Weiqing Xu et al.   BIOPHYSICAL JOURNAL

Dimensional reduction for single-molecule imaging of condensation events

(2022) Nils A. Benning et al.   BIOPHYSICAL JOURNAL

Next generation single-molecule techniques: Imaging, labeling, and manipulation in vitro and in cellulo

(2022) Taekjip Ha et al.   MOLECULAR CELL

Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates

(2022) Janine Hochmair et al.   EMBO JOURNAL

Receptor tyrosine kinases regulate signal transduction through a liquid-liquid phase separated state

(2022) Chi-Chuan Lin et al.   MOLECULAR CELL

Membrane surfaces regulate assembly of ribonucleoprotein condensates

(2022) Wilton T. Snead et al.   NATURE CELL BIOLOGY

Dilute phase oligomerization can oppose phase separation and modulate material properties of a ribonucleoprotein condensate

(2022) Ian Seim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses

(2022) Jonathan X. Meng et al.   Nature Communications

Structure-dependent recruitment and diffusion of guest proteins in liquid droplets of FUS

(2022) Kiyoto Kamagata et al.   Scientific Reports

Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains

(2022) Thuy P Dao et al.   EMBO REPORTS

Dynamin is primed at endocytic sites for ultrafast endocytosis

(2022) Yuuta Imoto et al.   NEURON

Phase-separating RNA-binding proteins form heterogeneous distributions of clusters in subsaturated solutions

(2022) Mrityunjoy Kar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ATP:Mg2+ shapes material properties of protein-RNA condensates and their partitioning of clients

(2022) N. Amy Yewdall et al.   BIOPHYSICAL JOURNAL

Small soluble α-synuclein aggregates are the toxic species in Parkinson’s disease

(2022) Derya Emin et al.   Nature Communications

Unravelling the microscopic characteristics of intrinsically disordered proteins upon liquid–liquid phase separation

(2022) Si Wu et al.   Essays in Biochemistry

Active microrheology of protein condensates using colloidal probe-AFM

(2022) Xiufeng Li et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

(2022) Fernando Muzzopappa et al.   Nature Communications

Different Forms of Disorder in NMDA-Sensitive Glutamate Receptor Cytoplasmic Domains Are Associated with Differences in Condensate Formation

(2022) Sujit Basak et al.   Biomolecules

Arg/Lys-containing IDRs are cryptic binding domains for ATP and nucleic acids that interplay to modulate LLPS

(2022) Mei Dang et al.   Communications Biology

A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

(2021) Joris Van Lindt et al.   Communications Biology

From Prions to Stress Granules: Defining the Compositional Features of Prion-Like Domains That Promote Different Types of Assemblies

(2021) Anastasia Fomicheva et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Single-molecule and ensemble methods to probe RNP nucleation and condensate properties

(2021) Kevin Rhine et al.   METHODS

An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors

(2021) David A. Garcia et al.   MOLECULAR CELL

Single molecule microscopy reveals key physical features of repair foci in living cells

(2021) Judith Miné-Hattab et al.   eLife

Dynamics of long-term protein aggregation on low-fouling surfaces

(2021) Mohammadhasan Hedayati et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

High-Speed Atomic Force Microscopy Reveals Spatiotemporal Dynamics of Histone Protein H2A Involution by DNA Inchworming

(2021) Goro Nishide et al.   Journal of Physical Chemistry Letters

A Close Look at Molecular Self-Assembly with the Transmission Electron Microscope

(2021) Aoon Rizvi et al.   CHEMICAL REVIEWS

Conformational Expansion of Tau in Condensates Promotes Irreversible Aggregation

(2021) Jitao Wen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A multi-step nucleation process determines the kinetics of prion-like domain phase separation

(2021) Erik W. Martin et al.   Nature Communications

Prion-Like Proteins in Phase Separation and Their Link to Disease

(2021) Macy L. Sprunger et al.   Biomolecules

Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells

(2021) Jason C. Sang et al.   Communications Biology

Single-molecule Detection of Ultrafast Biomolecular Dynamics with Nanophotonics

(2021) Mark F. Nüesch et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates

(2021) Ryanggeun Lee et al.   NUCLEIC ACIDS RESEARCH

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters

(2020) Tatjana Trcek et al.   MOLECULAR CELL

On the Aggregation and Nucleation Mechanism of the Monoclonal Antibody Anti-CD20 Near Liquid-Liquid Phase Separation (LLPS)

(2020) Elvira Pantuso et al.   Scientific Reports

Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells

(2020) Simao Coelho et al.   Science Advances

Determinants for Fusion Speed of Biomolecular Droplets

(2020) Archishman Ghosh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

(2020) Anne-Marie Ladouceur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dehydration entropy drives liquid-liquid phase separation by molecular crowding

(2020) Sohee Park et al.   Communications Chemistry

Phase separation at the nanoscale quantified by dcFCCS

(2020) Sijia Peng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The hierarchical assembly of septins revealed by high-speed AFM

(2020) Fang Jiao et al.   Nature Communications

Phase-Separated Transcriptional Condensates Accelerate Target-Search Process Revealed by Live-Cell Single-Molecule Imaging

(2020) Samantha Kent et al.   Cell Reports

Optical Tweezers Approaches for Probing Multiscale Protein Mechanics and Assembly

(2020) Kathrin Lehmann et al.   Frontiers in Molecular Biosciences

Regulatory mechanisms of tau protein fibrillation under the conditions of liquid–liquid phase separation

(2020) Solomiia Boyko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein condensates as aging Maxwell fluids

(2020) Louise Jawerth et al.   SCIENCE

Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts

(2020) Nicky Atkinson et al.   Nature Communications

Multicolour single-molecule tracking of mRNA interactions with RNP granules

(2019) Stephanie L. Moon et al.   NATURE CELL BIOLOGY

Using enhanced number and brightness to measure protein oligomerization dynamics in live cells

(2019) Francesco Cutrale et al.   Nature Protocols

The role of liquid-liquid phase separation in aggregation of the TDP-43 low complexity domain

(2019) W. Michael Babinchak et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS

(2019) Desiree M Baron et al.   HUMAN MOLECULAR GENETICS

Time-Resolved Observations of Liquid–Liquid Phase Separation at the Nanoscale Using in Situ Liquid Transmission Electron Microscopy

(2019) Hortense Le Ferrand et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

Loss of Dynamic RNA Interaction and Aberrant Phase Separation Induced by Two Distinct Types of ALS/FTD-Linked FUS Mutations

(2019) Amirhossein Ghanbari Niaki et al.   MOLECULAR CELL

Properties of Stress Granule and P-Body Proteomes

(2019) Ji-Young Youn et al.   MOLECULAR CELL

Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization

(2019) Ying Xie et al.   Nature Communications

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

(2016) Krishna C Mudumbi et al.   Nature Communications

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE