Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus

(2020) Keren Lasker et al.   Nature Microbiology

SMAUG: Analyzing single-molecule tracks with nonparametric Bayesian statistics

(2020) Joshua D. Karslake et al.   METHODS

More than just a phase: the search for membraneless organelles in the bacterial cytoplasm

(2019) Elio A. Abbondanzieri et al.   CURRENT GENETICS

Cellular sensing by phase separation: Using the process, not just the products

(2019) Haneul Yoo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality

(2019) Sethuramasundaram Pitchiaya et al.   MOLECULAR CELL

SuhB is an integral part of the ribosomal antitermination complex and interacts with NusA

(2019) Benjamin R Dudenhoeffer et al.   NUCLEIC ACIDS RESEARCH

Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis

(2019) Yong-Heng Huang et al.   NUCLEIC ACIDS RESEARCH

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

The bacterial cell cycle, chromosome inheritance and cell growth

(2019) Rodrigo Reyes-Lamothe et al.   NATURE REVIEWS MICROBIOLOGY

Phase separation and clustering of an ABC transporter in Mycobacterium tuberculosis

(2019) Florian Heinkel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chromatin topology, condensates and gene regulation: shifting paradigms or just a phase?

(2019) Mustafa Mir et al.   DEVELOPMENT

Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus

(2019) Run-Wen Yao et al.   MOLECULAR CELL

DEAD-box ATPases are global regulators of phase-separated organelles

(2019) Maria Hondele et al.   NATURE

Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli

(2019) Xiaoli Weng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules

(2018) Benedikt Niewidok et al.   JOURNAL OF CELL BIOLOGY

Structural Basis for NusA Stabilized Transcriptional Pausing

(2018) Xieyang Guo et al.   MOLECULAR CELL

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Transcriptional Responses to ppGpp and DksA

(2018) Richard L. Gourse et al.   Annual Review of Microbiology

Global DNA Compaction in Stationary-Phase Bacteria Does Not Affect Transcription

(2018) Richard Janissen et al.   CELL

α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies

(2018) Nadra Al-Husini et al.   MOLECULAR CELL

Bacterial FtsZ protein forms phase‐separated condensates with its nucleoid‐associated inhibitor SlmA

(2018) Begoña Monterroso et al.   EMBO REPORTS

TrackMate: An open and extensible platform for single-particle tracking

(2017) Jean-Yves Tinevez et al.   METHODS

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enrichment of dynamic chromosomal crosslinks drive phase separation of the nucleolus

(2017) Caitlin Hult et al.   NUCLEIC ACIDS RESEARCH

Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation inPseudomonas aeruginosa

(2017) Lisa R. Racki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell

(2017) Markus Sutter et al.   SCIENCE

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Frequent exchange of the DNA polymerase during bacterial chromosome replication

(2017) Thomas R Beattie et al.   eLife

Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers

(2016) Yi Lin et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Colocalization of distant chromosomal loci in space in E. coli : a bacterial nucleolus

(2016) Tamas Gaal et al.   GENES & DEVELOPMENT

Transcription is regulated by NusA:NusG interaction

(2016) Martin Strauß et al.   NUCLEIC ACIDS RESEARCH

Polyhydroxyalkanoate (PHA) Granules Have no Phospholipids

(2016) Stephanie Bresan et al.   Scientific Reports

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

Inverse Size Scaling of the Nucleolus by a Concentration-Dependent Phase Transition

(2015) Stephanie C. Weber et al.   CURRENT BIOLOGY

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

RNA transcription modulates phase transition-driven nuclear body assembly

(2015) Joel Berry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Live-cell superresolution microscopy reveals the organization of RNA polymerase in the bacterial nucleoid

(2015) Mathew Stracy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

(2015) Sonja Kroschwald et al.   eLife

Endosymbiotic theory for organelle origins

(2014) Verena Zimorski et al.   CURRENT OPINION IN MICROBIOLOGY

Genome-scale quantitative characterization of bacterial protein localization dynamics throughout the cell cycle

(2014) Nathan J. Kuwada et al.   MOLECULAR MICROBIOLOGY

Spatial organization of transcription machinery and its segregation from the replisome in fast-growing bacterial cells

(2014) Cedric Cagliero et al.   NUCLEIC ACIDS RESEARCH

Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging

(2014) S. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Partitioning of RNA Polymerase Activity in Live Escherichia coli from Analysis of Single-Molecule Diffusive Trajectories

(2013) Somenath Bakshi et al.   BIOPHYSICAL JOURNAL

Multiscale Spatial Organization of RNA Polymerase in Escherichia coli

(2013) Ulrike Endesfelder et al.   BIOPHYSICAL JOURNAL

Four-Dimensional Imaging of E. coli Nucleoid Organization and Dynamics in Living Cells

(2013) Jay K. Fisher et al.   CELL

The Mitochondrial RNA-Binding Protein GRSF1 Localizes to RNA Granules and Is Required for Posttranscriptional Mitochondrial Gene Expression

(2013) Hana Antonicka et al.   Cell Metabolism

Morphology and function of membrane-bound organelles

(2013) Rebecca Heald et al.   CURRENT OPINION IN CELL BIOLOGY

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Getting RNA and Protein in Phase

(2012) Stephanie C. Weber et al.   CELL

Variation of the folding and dynamics of theEscherichia colichromosome with growth conditions

(2012) Nastaran Hadizadeh Yazdi et al.   MOLECULAR MICROBIOLOGY

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Structure of the SSB-DNA polymerase III interface and its role in DNA replication

(2011) Aimee H Marceau et al.   EMBO JOURNAL

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cardiolipin microdomains localize to negatively curved regions of Escherichia coli membranes

(2011) L. D. Renner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

NusA Interaction with the α Subunit of E. coli RNA Polymerase Is via the UP Element Site and Releases Autoinhibition

(2011) Kristian Schweimer et al.   STRUCTURE

The NusA N-Terminal Domain Is Necessary and Sufficient for Enhancement of Transcriptional Pausing via Interaction with the RNA Exit Channel of RNA Polymerase

(2010) Kook Sun Ha et al.   JOURNAL OF MOLECULAR BIOLOGY

Bacterial Chromosomal Loci Move Subdiffusively through a Viscoelastic Cytoplasm

(2010) Stephanie C. Weber et al.   PHYSICAL REVIEW LETTERS

Strong intranucleoid interactions organize the Escherichia coli chromosome into a nucleoid filament

(2010) P. A. Wiggins et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interdependence of Cell Growth and Gene Expression: Origins and Consequences

(2010) M. Scott et al.   SCIENCE

The Role of Disordered Ribosomal Protein Extensions in the Early Steps of Eubacterial 50 S Ribosomal Subunit Assembly

(2009) Youri Timsit et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

RNA-binding specificity of E. coli NusA

(2009) S. Prasch et al.   NUCLEIC ACIDS RESEARCH

Quantitative genome-scale analysis of protein localization in an asymmetric bacterium

(2009) J. N. Werner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Stress induces the assembly of RNA granules in the chloroplast ofChlamydomonas reinhardtii

(2008) James Uniacke et al.   JOURNAL OF CELL BIOLOGY

Protein Disorder Is Positively Correlated with Gene Expression inEscherichia coli

(2008) Oleg Paliy et al.   JOURNAL OF PROTEOME RESEARCH

Growth-rate-dependent partitioning of RNA polymerases in bacteria

(2008) S. Klumpp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA