Article
Engineering, Chemical
Wei Gao, Feng Zhang, Sen Zhang, Jia-yuan Li, Hong-zhen Lian
Summary: High selective enrichment of low abundance phosphopeptides from complex biological samples is essential for in-depth phosphoproteomics. A magnetic material called mMOF-FBP-Ti4+, fabricated by grafting UiO-66-NH2 metal-organic framework onto the surface of Fe3O4 and immobilizing Ti(IV) with fructose 1,6-bisphosphate, exhibited favorable properties for phosphopeptide enrichment. The material demonstrated strong affinities through metal oxide affinity chromatography and immobilized metal affinity chromatography, as well as improved hydrophilicity for enhanced hydrophilic chromatography interaction. The mMOF-FBP-Ti4+ also yielded low detection limit, high selectivity, good reusability, and high postenrichment recovery towards phosphopeptides, making it a promising tool for efficient enrichment in complex biological samples.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jacob H. Forstater, Shane T. Grosser
Summary: Immobilization technology plays a significant role in commodity chemical synthesis and pharmaceutical synthesis. This study presents a data-rich approach that automates the screening and development of affinity-based enzyme immobilizations, enabling real-time process characterization and rapid decision making. The developed methodology reduces the time and resources required for immobilization process development and optimization.
REACTION CHEMISTRY & ENGINEERING
(2022)
Article
Biochemical Research Methods
Tomoto Ura, Tomoshi Kameda, Fina A. Laksmi, Matsujiro Ishibashi, Tsutomu Arakawa, Kentaro Shiraki, Atsushi Hirano
Summary: Immobilized metal ion affinity chromatography (IMAC) is a useful method for purifying histidine-tagged or histidine-rich proteins and peptides. However, phenolic compounds interfere with IMAC purification of proteins from plant-based host systems. This study investigated the affinity of phenolic compounds for transition metal ions and found that the more hydroxyl groups a compound has, the higher its affinity for transition metal ions. Importantly, phenolic compounds showed no obvious affinity for the Ni(II)-IMAC column, while imidazole and histidine-tagged proteins demonstrated binding to the Ni(II)-IMAC column.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Wenrui Wu, Ruizhi Tang, Zhouxian Li, Yehua Shen, Shujuan Ma, Junjie Ou
Summary: A Ti4+-immobilized polydispersed phosphate-rich microsphere was successfully fabricated using inverse suspension polymerization method, showing excellent sensitivity, selectivity, and adsorption capacity. This method requires only two steps to synthesize Ti4+-IMAC, offering a simpler and more efficient approach for protein phosphorylation research and potential for pilot production and commercialization.
Article
Chemistry, Analytical
Dandan Jiang, Siyu Wu, Siqi Lv, Ruixue Qi, Yangyang Li, Jinghai Liu
Summary: A new chelator, L-Alanyl-L-Glutamine, was used to prepare immobilized magnetic graphite nitride material with cerium ions. The resulting nanocomposite showed high surface area, hydrophilicity, positive electricity, and magnetic response. As an immobilized metal ion affinity chromatography sorbent, it exhibited high sensitivity, selectivity, and recyclability. The analysis of A549 cell lysate identified numerous phosphopeptides mapped to phosphoproteins.
Article
Chemistry, Applied
Qing Shen, Huanming Wu, Honghai Wang, Qiaoling Zhao, Jing Xue, Jianfeng Ma, Haixing Wang
Summary: A method using MM-IMAC material for enrichment and analysis of phospholipids in krill was developed, showing good linearity, sensitivity, and precision, suitable for phospholipid studies in food matrices.
Article
Chemistry, Analytical
Dandan Jiang, Siqi Lv, Xue Han, Limei Duan, Jinghai Liu
Summary: Exfoliated magadiite nanosheets embedded with Fe3O4 were utilized to prepare Gd3+-immobilized magnetic magadiite nanosheets as an adsorbent for phosphopeptides enrichment. The 2D morphology of the adsorbent provided high surface area and abundant Gd3+ contents, leading to efficient phosphopeptides capture with low detection limit and stable enrichment performance. Further evaluation demonstrated the capability of the nanocomposite to enrich phosphopeptides from various biological samples.
Review
Infectious Diseases
Ligang Liu, Milap C. C. Nahata
Summary: Helicobacter pylori is a common cause of infections worldwide and its resistance to antibiotics is increasing. Amoxicillin is the preferred treatment, but many patients have penicillin allergy. For patients with true allergy, Vonoprazan-Clarithromycin-Metronidazole and bismuth quadruple therapy have shown excellent eradication rates. Vonoprazan-based therapy is better tolerated and can be considered as first-line treatment. Levofloxacin or sitafloxacin-based regimens have moderate eradication rates but potential serious adverse effects. Cephalosporins like cefuroxime can be an alternative to amoxicillin. Choosing the most effective antibiotic regimen can improve clinical outcomes in H. pylori infection with penicillin allergy.
Review
Biochemistry & Molecular Biology
Ho-Yu Ng, Wai K. Leung, Ka-Shing Cheung
Summary: Despite the global decline in prevalence, efforts are needed to optimize H. pylori treatment regimens due to increasing antibiotic resistance. Advancements in sequencing technology have helped broaden our understanding of molecular mechanisms behind resistance and facilitated testing of antibiotic susceptibility. Establishing routine antibiotic susceptibility surveillance programs and enforcing antibiotic stewardship are important for managing H. pylori in a sustainable manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Kexuan Wang, Ajuan Yu, Yu Gao, Miao Chen, Hang Yuan, Shusheng Zhang, Gangfeng Ouyang
Summary: A novel immobilized metal affinity chromatography (IMAC) functional composite, mNi@N-GrT@PDA@Ti4+, was fabricated for selective enrichment and mass spectrometry identification of phosphorylated peptides. The composite showed high specificity and low detection limits in the enrichment of phosphopeptides from protein digest mixtures. It also demonstrated successful selective enrichment of phosphopeptides in complex biological samples.
Review
Chemistry, Analytical
Cigdem Kip, Kadriye Ozlem Hamaloglu, Cihan Demir, Ali Tuncel
Summary: Isolation or enrichment of biological molecules from complex biological samples is often a prerequisite in proteomics, genomics, and glycomics. Among various techniques, bioaffinity chromatography stands out as a powerful method for isolating target biological molecules through specific interactions with ligands immobilized on support materials. Recent developments in bioaffinity chromatography, including properties of supports, immobilization techniques, types of binding agents, and application methods, have been summarized in the literature.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Karina A. Baksh, Dmitry Pichugin, Robert Scott Prosser, Deborah B. Zamble
Summary: Nickel plays a crucial role in the survival of the pathogenic bacteria Helicobacter pylori in the human stomach by regulating nickel homeostasis through the transcription factor NikR. Nickel binding induces conformational changes in HpNikR, enabling it to bind specific DNA motifs and influence gene transcription. Zinc is unable to induce the same allosteric response as nickel, suggesting that the mechanism of nickel-activated DNA binding by HpNikR is driven by conformational selection.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dandan Jiang, Siqi Lv, RuiXue Qi, Xue Han, Limei Duan, Jinghai Liu
Summary: This study developed a layer-by-layer assembly strategy to prepare multilayered magnetic nanoparticles with double hydroxide/polyoxometalate shell coatings. The introduction of functional shells increased the surface area for target contact and provided abundant affinity sites. The nanomaterial showed excellent performance in capturing phosphopeptides with low detection limit, satisfactory selectivity, good recovery, high adsorption capacity, and ten times reusability.
Review
Materials Science, Biomaterials
Yuan Qin, Yeh-Hsing Lao, Haixia Wang, Jiabin Zhang, Ke Yi, Zhuanggui Chen, Jing Han, Wantong Song, Yu Tao, Mingqiang Li
Summary: H. pylori infection is the main cause of most digestive diseases, and traditional oral medication is limited in its efficacy, while nanomedicine offers efficient and effective targeted drug delivery solutions.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Analytical
Hongwei Wang, Ruizhi Tang, Shicong Jia, Shujuan Ma, Bolin Gong, Junjie Ou
Summary: This paper introduces a novel monodisperse MAR microsphere (Ti4+-Brush@MAR) prepared and modified via surface-initiated atom transfer radical polymerization (SI-ATRP), showing high enrichment specificity for phosphopeptides in beta-casein or milk digest samples.
Article
Environmental Sciences
Haowen Jiang, Ying Wang, Zhiqiang Tan, Ligang Hu, Jianbo Shi, Guangliang Liu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: This study developed an online technique that combines hollow fiber ultrafiltration (HFUF) with single particle-inductively coupled plasma mass spectrometry (SP-ICP-MS) to improve the accuracy and size detection limit of metal-containing nanoparticles (MCNs) by removing metal ions. Through systematic optimization of conditions, HFUF can completely remove metal ions while retaining the particles, allowing accurate size determination.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Environmental Sciences
Dingyi Wang, Tingting Zhang, Xudong Guo, Dayi Ling, Ligang Hu, Guibin Jiang
Summary: Reducing fossil fuel usage is crucial for achieving carbon neutrality, but current energy policies may hinder economic development and people's livelihood. Three-dimensional printing (3DP) is an environmentally friendly manufacturing technology with the potential to contribute to carbon neutrality. It can reduce greenhouse gas emissions in manufacturing and construction sectors, enable the development of novel energy-related devices and technologies, and promote the production of cultured meat.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Chemistry, Analytical
Xiangwei Tian, Haowen Jiang, Meng Wang, Wenbin Cui, Yingying Guo, Lingna Zheng, Ligang Hu, Guangbo Qu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: To meet the demand for multi-element/isotope analysis at the single nanoparticle (NP) or cell level, different types of inductively coupled plasma mass spectroscopy (ICP-MS) have been used to simultaneously monitor multiple mass-to-charge ratios in single-particle/cell ICP-MS (SP/SC-ICP-MS) analysis. Systematic evaluation and comparison of the performance of these techniques are urgently required.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Qing Yang, Mengzhu Cheng, Qing Zhou, Lihong Wang, Yunlong Jiao, Yongqiang Liu, Shuya Zhang, Li Tan, Zhenhong Gu, Hong Zhu, Hongli Luo, Daozhe Lin, Nian Liu, Xiaohua Huang, Ligang Hu
Summary: The accumulation of various pollutants in leaf cells of different plants in Nanjing was significantly higher than those grown in Haikou, especially for BaP, Cd, and REEs. The activation of endocytosis in leaf cells by REEs led to the co-accumulation of BaP, Cd, and REEs, magnifying their toxic effects on plants. Moreover, the co-accumulation of pollutants in human cells was also increased by REEs.
Article
Environmental Sciences
Yaqian Xu, Yu Hu, Xin Wang, Xianping Wei, Qingqing Zhu, Ligang Hu, Chunyang Liao, Guibin Jiang
Summary: The aim of this study was to investigate the exposure of novel high-molecular-weight synthetic antioxidants in children's urine from different regions in China. The study found that the analytes were detected in children's urine, and significant differences were observed in the concentrations of certain synthetic antioxidants between the eastern and western regions of China. The study also assessed the health risk of the synthetic antioxidants and found that the estimated daily intakes were lower than the tolerable daily intake values, indicating a low health risk to children.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Shiyu Li, Lin Yang, Jialong Wu, Linlin Yao, Deming Han, Yong Liang, Yongguang Yin, Ligang Hu, Jianbo Shi, Guibin Jiang
Summary: Developing practical and cost-effective adsorbents for mercury (Hg) remediation is crucial for protecting the aquatic environment and public health. In this study, a recyclable hierarchical MoS2/Fe3O4 nanocomposite was developed for the selective removal of Hg(II) from aquatic samples. The nanocomposite exhibited high adsorption capacity, fast kinetics, broad working pH range, excellent selectivity, and great reusability. It showed promising efficiency in removing Hg(II) from natural water and industrial effluents. The study provides insights into the mechanism of Hg(II) removal and highlights the potential of MoS2/Fe3O4 for sustainable water treatments.
Article
Multidisciplinary Sciences
Haibo Wang, Ligang Hu, Hongyan Li, Yau-Tsz Lai, Xueying Wei, Xiaohan Xu, Zhenkun Cao, Huiming Cao, Qianya Wan, Yuen-Yan Chang, Aimin Xu, Qunfang Zhou, Guibin Jiang, Ming-Liang He, Hongzhe Sun
Summary: In this study, the researchers identified the Cr(III)-proteome in cells and found that ATP synthase plays a crucial role in relieving hyperglycemia stress.
NATURE COMMUNICATIONS
(2023)
Article
Environmental Sciences
Ke Yuan, Lan Lin, Xiuqin Xie, Zhiqiang Zhao, Ligang Hu, Tiangang Luan, Baowei Chen
Summary: In this study, metagenomic approaches were used to analyze water samples collected from the Pearl River and Pearl River Estuary for the presence of pathogenic bacteria. A total of 71 putative pathogenic bacterial species were identified, with Bacteroidetes being the predominant phylum. The concentration of pathogenic bacteria ranged from 1.59 x 107 to 1.46 x 108 cells/L, with a decreasing trend from the Pearl River to the Pearl River Estuary. Pathogenic particle-attached bacteria accounted for a significant proportion of the total pathogenic bacteria, and various virulence factor genes were detected, with those related to bacterial adherence being the most abundant. This study highlights the importance of suspended particulate matters in the transport of pathogenic bacteria and suggests that particle-attached bacteria may contribute to potential health risks.
Review
Chemistry, Analytical
Junpeng Zhang, Dingyi Wang, Yingying Li, Lihong Liu, Yong Liang, Bin He, Ligang Hu, Guibin Jiang
Summary: The latest progress in 3D printing technology in the field of environmental analysis is comprehensively reviewed in this paper. By optimizing printing materials, enhancing equipment structure, and integrating multidisciplinary technology, 3D printing offers opportunities to solve the challenges faced by traditional processing techniques and promote the high performance and versatility of environmental analysis devices.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Lei Li, Anthony S. Wexler, Xue Li, Ligang Hu, Guibin Jiang
Summary: Bioaerosol transmission is an important pathway for COVID-19 and other infectious respiratory diseases caused by viral infection. In situ and real-time detection of bioaerosols and their encapsulated pathogens is crucial for early warning and monitoring of epidemics and pandemics. However, the lack of a powerful analytical tool for distinguishing between bioaerosols and nonbioaerosols, as well as identifying pathogen species, is a major challenge in this field.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Hongzhe Dong, Lihong Liu, Qinfei Zhou, Yinyin Tang, Huiling Wang, Yongguang Yin, Jianbo Shi, Bin He, Yanbin Li, Ligang Hu, Guibin Jiang
Summary: This study investigated the formation, distribution, and excretion of mercury nanoparticles in diatom Chaetoceros curvisetus, and found that these nanoparticles play crucial roles in understanding mercury fate in the ocean and its burial in sediments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Yuping Xiang, Yingying Guo, Guangliang Liu, Yanwei Liu, Maoyong Song, Jianbo Shi, Ligang Hu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: In this study, a whole-cell fluorescent biosensor based on Escherichia coli was developed to investigate the bioavailability and microbial activation process of mercury sulfide nanoparticles (HgSNPs). The results showed that HgSNPs had high bioavailability and were partially dissolved inside E. coli cells. The findings highlight the importance of microbial uptake and dissolution of HgSNPs.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Ying Wang, Guangliang Liu, Yingying Fang, Peng Liu, Yanwei Liu, Yingying Guo, Jianbo Shi, Ligang Hu, Yong Cai, Yongguang Yin, Guibin Jiang
Summary: This study verifies the importance of mercurous species (Hg(I)) in natural water contaminated by liquid elemental mercury droplet (Hg(0)l) and in simulating the dark oxidation process of Hg(0)l. The formation and transformation of Hg(I) control the overall oxidation of Hg(0)l and are influenced by environmental factors. The conversion of Hg(0) to Hg(I) is the rate-limiting step in the oxidation process, and ligands like OH-, Cl-, and natural organic matter can enhance the formation of Hg(I).
Article
Engineering, Environmental
Zelin Shen, Guangliang Liu, Yingying Guo, Tao Jiang, Yanwei Liu, Jianbo Shi, Ligang Hu, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: The aging kinetics of divalent mercury (Hg(II)) mediated by dissolved organic matter (DOM) play a significant role in controlling Hg(II) transformation and bioavailability in natural aquatic environments. However, the differential behaviors of new and aged Hg(II) in the same reaction system remain unknown. This study used multi-isotope tracing to investigate the impacts of binding site and sulfidation during DOM-mediated Hg(II) aging processes on Hg(II) reduction and microbial methylation in the same reaction system.
Article
Engineering, Environmental
Xiaoyan Zhang, Yingying Guo, Guangliang Liu, Yanwei Liu, Jianbo Shi, Ligang Hu, Lixia Zhao, Yanbin Li, Yongguang Yin, Yong Cai, Guibin Jiang
Summary: The widely distributed marine bacterium Alteromonas sp. KD01 is capable of extracellularly reducing Hg(II) through a superoxide-dependent mechanism. The generation of superoxide by Alteromonas sp. KD01 and the role of NADH in Hg(II) reduction were demonstrated. This finding provides insights into Hg(II) reduction by superoxide in various aerobic environments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
