期刊
MACROMOLECULAR RAPID COMMUNICATIONS
卷 37, 期 6, 页码 551-557出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201500705
关键词
enzyme immobilization; layer growth; macroporous polymer monolith; metal-organic framework; self-assembly
资金
- National Natural Science Foundation of China [21306006, 21576017, 21436002]
- 973 programs [2014CB745103]
- 863 programs [2015BAD15B07]
- Xiamen Scientific and Technological project [3502Z20142012]
- Public Hatching Platform for Recruited Talents of Beijing University of Chemical Technology
- Fundamental Research Funds for the Central Universities [buctrc201314, YS1407, buctrc201413]
New monolithic materials comprising zeolitic imidazolate framework (ZIF-8) located on the pore surface of poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith previously functionalized with N-(3-aminopropyl)-imidazole have been prepared via a layer-by-layer self-assembly strategy. These new ZIF-8@monolith hybrids are used as solid-phase carriers for enzyme immobilization. Their performance is demonstrated with immobilization of a model proteolytic enzyme trypsin. The best of the conjugates enable very efficient digestion of proteins that can be achieved in mere 43s.
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